Rona Chandrawati

Rona Chandrawati
UNSW Sydney | UNSW · School of Chemical Engineering

PhD (Bionanotechnology), BSc (Biotechnology)

About

91
Publications
16,137
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
3,820
Citations

Publications

Publications (91)
Article
Full-text available
Autoimmune diseases and in particular type 1 diabetes rely heavily on treatments that target the symptoms rather than prevent the underlying disease. One of the barriers to better therapeutic strategies is the inability to detect and efficiently target rare autoreactive T-cell populations that are major drivers of these conditions. Here, we develop...
Article
Full-text available
Gallium (Ga) compounds, as the source of Ga ions (Ga3+), have been historically used as anti-inflammatories. Currently, the widely accepted mechanisms of the anti-inflammatory effects for Ga3+ are rationalized on the basis of their similarities to ferric ions (Fe3+), which permits Ga3+ to bind with Fe-binding proteins and subsequently disturbs the...
Article
Polydiacetylene (PDA) sensors have been developed as food management tools due to their convenient colorimetric signal, low cost, versatility, and ability to be incorporated into food packaging materials and devices. In this review, we discuss methods of PDA sensor fabrication, their use as food quality and food contaminant sensors, and their incor...
Article
Full-text available
Hydrogen sulfide (H2S) is a gaseous molecule involved in multiple biological and physiological processes, including various diseases such as cancer and neurodegenerative disorders. This has led to the development of various analytical methods to monitor H2S in biological settings. Among these, fluorescence‐based assays, specifically organic small‐m...
Article
Ceria nanoparticles (NPs) are widely reported to scavenge nitric oxide (NO) radicals. This study reveals evidence that an opposite effect of ceria NPs exists, that is, to induce NO generation. Herein, S-nitrosoglutathione (GSNO), one of the most biologically abundant NO donors, is catalytically decomposed by ceria NPs to produce NO. Ceria NPs maint...
Article
Hydrogen sulfide (H2S) is a gasotransmitter known to regulate physiological and pathological processes. Abnormal H2S levels have been associated with a range of conditions, including Parkinson's and Alzheimer's diseases, cardiovascular and renal diseases, bacterial and viral infections, as well as cancer. Therefore, fast and sensitive H2S detection...
Article
Zinc oxide (ZnO) has emerged as a promising material for nitric oxide (NO) delivery owing to its intrinsic enzyme-mimicking activities to catalyze NO prodrugs S-nitrosoglutathione (GSNO) and β-gal-NONOate for NO generation. The catalytic performance of enzyme mimics is strongly dependent on their size, shape, and surface chemistry; however, no stud...
Article
Neurodegenerative disorders (NDDs) are progressive, incurable health conditions that primarily affect brain cells, and result in loss of brain mass and impaired function. Current sensing technologies for NDD detection are limited by high cost, long sample preparation, and/or require skilled personnel. To overcome these limitations optical sensors,...
Book
Recent developments in novel materials, sensing principles and signal processing are paving the way for new types of analytical sensors, offering an improved sensing performance and practicality. Such advancements will provide accurate and real-time analytical information in the environmental, food, and healthcare sectors to better guide decision-m...
Article
Full-text available
Coronavirus disease 2019 (COVID‐19) is an infectious disease caused by the severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) that has quickly and deeply affected the world, with over 60 million confirmed cases. There has been a great effort worldwide to contain the virus and to search for an effective treatment for patients who become cr...
Article
Polydiacetylenes (PDAs) have been widely used for sensing chemical and biological targets. The solvent injection (SI) method has been proposed as a viable alternative to the traditional thin film hydration method for large-scale PDA particle production, however the synthesis of PDA particles by SI remains understudied. The aim of the present study...
Article
Food sensors have been developed for quality monitoring and contaminant detection to improve food management. Despite the alarming rates of food waste and food‐related illness, few food sensor technologies are utilized and translated into commercial products. This review aims to explore challenges and opportunities relating to sensor translation by...
Article
Nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S) are gaseous signaling molecules (gasotransmitters) that regulate both physiological and pathological processes and offer therapeutic potential for the treatment of many diseases, such as cancer, cardiovascular disease, renal disease, bacterial and viral infections. However, the inh...
Article
A vast range of biomedical applications relies on the specificity of interactions between an antigen and its cognate receptor or antibody. This specificity can be highest when said antigen is a non-natural (synthetic) molecule introduced into a biological setting as a bio-orthogonal ligand. This review aims to present the development of this method...
Article
Membrane fusion is a highly evolved and significant physiological process involving the mixing of two initially distinct lipid bilayer membranes. It is concerned with communication between and within cells and plays a key role in various processes such as exocytosis, endocytosis, fertilization, viral infection, and carcinogenesis. Due to its signif...
Article
The incorporation of colorimetric sensors as quality indicators in food packaging is an exciting new area of research that could improve food management. The standard approach, however, demands a reliable interface between the sensor and the food and risks food contamination which is a significant consumer concern. To overcome this challenge, herei...
Preprint
Autoimmune diseases and in particular type 1 diabetes rely heavily on treatments that target the symptoms rather than prevent the underlying disease. One of the barriers to better therapeutic strategies is the inability to detect and efficiently target rare autoreactive T-cell populations that are major drivers of these conditions. Here, we develop...
Article
Nitric oxide (NO) is an essential signaling molecule with a number of biological functions and holds great promise in biomedical applications. However, NO delivery technologies have been complicated due to the inherent properties of NO which include short half-life and limited transport distance in human tissues. In addition, the biofunctionality o...
Article
Early diagnosis of mastitis is essential to reduce the expenses it imparts on the dairy industry. Existing techniques fail to provide rapid and pathogen-specific information to direct the prescription of antibiotics. Polydiacetylene (PDA)-based colorimetric sensors have emerged as promising bacterial screening tools that show potential for applicat...
Article
Polydiacetylenes (PDAs) are a class of conjugated polymers with unique optical properties that have practical applications in a diverse range of fields. PDA vesicular particles are typically constructed using a thin film hydration method which suffers from a lack of scalability and requires techniques such as sonication and extrusion to control par...
Article
Ethylene is a hormone that plays a critical role in many phases of plant growth and fruit ripening. Currently, detection of ethylene relies heavily on sophisticated and time-consuming conventional assays such as chromatography, spectroscopy, and electrochemical methods. Herein, we develop a polydiacetylene-based sensor for the detection of ethylene...
Article
Listeria monocytogenes is an important foodborne pathogen responsible for foodborne listeriosis, a significant issue for high risk groups such as pregnant women. Currently available detection techniques for this bacterium, primarily nucleic acid-based methods, can achieve low detection limits, however the assays are generally complex and lengthy. T...
Article
Existing date marking tools, such as use‐by and sell‐by dates, fail to inform decision‐making throughout food distribution because they do not adapt to storage conditions such as temperature. Polymer‐based sensors can be incorporated into food packaging to provide an indication of food quality in real time, which can greatly reduce waste. This work...
Article
Active use-by date (AUBD) or freshness indicators hold great potential to reduce food waste. Herein, we develop an anthocyanin AUBD indicator that is capable of discriminating between fresh, spoiling, and spoiled milk. The sensor undergoes a visible blue to purple to pink color change in response to lactic acid, which is an indicator of microbial s...
Article
The highly diverse biological roles of nitric oxide (NO) in both physiological and pathophysiological processes have prompted great interest in the use of NO as a therapeutic agent in various biomedical applications. NO can exert either protective or deleterious effects depending on its concentration and the location where it is delivered or genera...
Chapter
This chapter discusses recent biosensor developments for the monitoring of food spoilage using polydiacetylene (PDA) and liposome‐based systems. Consequently, PDA is gaining attention as a colorimetric indicator of food spoilage throughout processing, distribution, and consumption. Typical food contaminants include bacteria, pesticides, allergens,...
Article
Early detection of cancer is likely to be one of the most effective means of reducing the cancer mortality rate. Hence, simple and ultra‐quick methods for noninvasive detection of early‐stage tumors are highly sought‐after. In this study, a nanobiosensing platform with a rapid response time of nearly 30 s is introduced for the detection of matrilys...
Article
Current food date marking tools fail to take into account variables that impact shelf life, leading to food waste and posing human health risks. Herein, we develop a polydiacetylene/zinc oxide (PDA/ZnO) colorimetric sensor that can indicate milk freshness in real time. The PDA/ZnO system is engineered to indicate the freshness of full cream milk by...
Article
Nitric oxide (NO) is a potent biological molecule that contributes to a wide spectrum of physiological processes. However, the full potential of NO as a therapeutic agent is significantly complicated by its short half‐life and limited diffusion distance in human tissues. Current strategies for NO delivery focus on encapsulation of NO donors into pr...
Article
Synthetic micromotors are evaluated extensively in a range of biomedical, microscale transport and environmental applications. Fundamental insight into micromotors that exhibit locomotion due to triggered disintegration of their associated liposomes is provided. Directed self-propulsion is observed when the lipid vesicles are solubilized using Trit...
Article
Full-text available
Enzyme-linked immunosorbent assay (ELISA) is a technique designed for the detection and quantification of (bio)molecules in a liquid sample. It is a powerful tool in clinical diagnostics, food safety and environmental monitoring. However, the main limitation of conventional ELISA is its low sensitivity, which cannot meet the demand of analyte analy...
Article
Polydiacetylenes (PDAs) are a class of conjugated polymers with unique optical properties that make them excellent materials for the construction of colorimetric sensors. Self-assembly of amphiphilic diacetylene monomers in solution and photopolymerization of neighboring diacetylene groups yield blue-phase PDAs. PDAs can be functionalized with a wi...
Chapter
Optical sensors have emerged as a suitable platform to monitor health parameters in a personalized fashion and they have the potential to revolutionize the way cancer is diagnosed and treated. Fluorescence readouts are particularly interesting for sensing applications as the changes in intensity or wavelength of light emitted after excitation with...
Article
The inclusion of nanomaterials into biopolymeric matrices followed by three-dimensional printing is poised to open new horizons in tissue engineering by generating customized 3D structures with optimized properties and multifunctionality. In this review, we present an overview on nanostructured ceramic, metal, carbon and polymer materials integrate...
Article
Nanozymes, which are functional nanomaterials with enzyme-like characteristics, have emerged as a highly-stable and low-cost alternative to natural enzymes. Apart from overcoming the limitations of natural enzymes (e.g., high cost, low stability or complex production), nanozymes are also equipped with the unique intrinsic properties of nanomaterial...
Article
Nanocellulose market is estimated to be worth $660 million by 2023. Rapid advances in nanotechnology and material sciences have facilitated investigation of nanocellulose, giving rise to their emergence as desirable biomaterials. There has been substantial research undertaken on the applications of nanocellulose in various fields such as renewable...
Article
Full-text available
Despite the promise of metal-organic frameworks (MOFs) as functional matrices for enzyme stabilization, the development of a stimulus-responsive approach to induce multi-enzyme cascade reaction in MOFs remains a critical challenge. Here, a novel method using peptide-induced super-assembly of MOFs is developed for programmed enzyme cascade reactions...
Article
In article number 1800330, Rona Chandrawati and Federico Mazur report the fusion of fluorophore‐containing liposomes mediated by complementary coiled‐coil‐forming peptides, which forms the basis of a detection assay for matrix metalloproteinases (MMPs). Fusion events are detected by an emission of light upon excitation of the donor fluorophores. In...
Article
Zinc oxide (ZnO) particles with different shapes and sizes have been previously reported to possess unique optical, electrical, photocatalytic, and antimicrobial properties. Capping agents are routinely used to control particle morphologies; however, few studies have evaluated the influence of capping agents on the growth kinetics of ZnO particles...
Article
Biological cells continue to inspire the development of technologies toward rapid, sensitive, and selective detection of analytes. Membrane fusion is a key biological event in living cells that involves a highly selective recognition mechanism controlled by different functional proteins. Herein, liposome–liposome fusion mediated by coiled‐coil form...
Article
Full-text available
Colorimetric gas sensors that detect early release of gases from food spoilage are of great importance in food safety and food conservation. Yet, such sensors are not broadly implemented as they are incompatible with food packaging and non-functional at the low temperatures at which food is stored. Here we report a low cost, highly sensitive ammoni...
Article
Full-text available
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a...
Article
Multi-compartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based microreactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA producti...
Article
Stimuli-responsive polymer materials are powerful tools in drug delivery and tissue engineering. Because of the large variations in physiological conditions between normal microenvironments and diseased sites, polymer materials with single responsiveness may not achieve the desired goals in a complex physiological microenvironment. Instead, polymer...
Article
Full-text available
Nitric oxide (NO) is a highly potent radical with a wide spectrum of physiological activities. Depending on the concentration, it can enhance endothelial cell proliferation in a growth factor‐free medium, mediate angiogenesis, accelerate wound healing, but may also lead to tumor progression or induce inflammation. Due to its multifaceted role, NO m...
Chapter
Polymer capsules fabricated via layer-by-layer (LbL) assembly have emerged as promising carriers for therapeutic delivery. The versatile assembly technique allows an extensive choice of materials to be incorporated as constituents of the multilayers, which therefore endow capsules with specific properties and functionalities. This chapter describes...
Article
Elevated cobalt levels in the bloodstream due to wear debris from metal-on-metal hip implants can cause adverse health effects. Herein, we report a highly sensitive and specific label-free assay for cobalt detection using cysteine-linked core-satellite gold nanoparticles. We demonstrate the detection of clinically relevant concentrations of cobalt...
Article
In article number 1706616, Molly M. Stevens and co‐workers present the incorporation of extracellular vesicles into biocompatible poly(vinyl alcohol)‐based hydrogels to advance the concept of enzyme‐prodrug therapy. Hydrogels are imaged by density‐dependent color scanning electron microscopy taking advantage of the backscattered signal of the label...
Article
Full-text available
Nitric oxide (NO) is a highly potent but short-lived endogenous radical with a wide spectrum of physiological activities. In this work, we developed an enzymatic approach to the site-specific synthesis of NO mediated by biocatalytic surface coatings. Multilayered polyelectrolyte films were optimized as host compartments for the immobilized β-galact...
Article
Full-text available
Extracellular vesicles (EVs) have recently gained significant attention as important mediators of intercellular communication, potential drug carriers, and disease biomarkers. These natural cell-derived nanoparticles are postulated to be biocompatible, stable under physiological conditions, and to show reduced immunogenicity as compared to other sy...
Article
The cover feature picture shows the fusion of fluorophore-containing liposomes, driven by the self-assembly of complementary DNA strands. Here, the DNA complexes are designed to respond to the presence of a specific microRNA sequence. In the presence of the target microRNA, the hairpin DNA (orange) is displaced to reveal a sticky end in double-stra...
Article
Full-text available
Membrane fusion is a process of fundamental importance in biological systems, involving highly selective recognition mechanisms for trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection, since relatively few highly discriminating...
Article
Full-text available
Quantitative detection of angiogenic biomarkers provides a powerful tool to diagnose cancers in early stages and to follow its progression during therapy. Conventional tests require trained personnel, dedicated laboratory equipment and are generally time-consuming. Herein, we propose our developed biosensing platform as a useful tool for a rapid de...
Article
Full-text available
In the past few years, magnetoelectronics has emerged as a promising new platform technology in various biosensors for detection, identification, localisation and manipulation of a wide spectrum of biological, physical and chemical agents. The methods are based on the exposure of the magnetic field of a magnetically labelled biomolecule interacting...
Article
We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection ar...
Article
Full-text available
Enzyme prodrug therapy (EPT) enables localized conversion of inert prodrugs to active drugs by enzymes. Performance of EPT necessitates that the enzyme remains active throughout the time frame of the envisioned therapeutic application. β-glucuronidase is an enzyme with historically validated performance in EPT, however it retains its activity in bi...
Article
Biosensors for the rapid, specific, and sensitive detection of analytes play a vital role in healthcare, drug discovery, food safety, and environmental monitoring. Although a number of sensing concepts and devices have been developed, many longstanding challenges to obtain inexpensive, easy-to-use, and reliable sensor platforms remain largely unmet...
Article
Full-text available
Nitric oxide (NO) is able to lower intraocular pressure (IOP); however, its therapeutic effects on outflow physiology are location- and dose-dependent. An NO delivery platform that directly targets the resistance-generating region of the conventional outflow pathway and locally liberates a controlled dose of NO is reported. An increase in outflow f...
Article
Full-text available
Statement of significance: Zirconia-toughened alumina (ZTA) is the current gold standard for the fabrication of orthopedic ceramic components. In the present work, we propose an innovative strategy to provide both osseointegrative and antibacterial properties to ZTA surfaces: we demonstrate that injection molding allows a flexible design of surfac...
Article
Enzymes play a central role in a spectrum of fundamental physiological processes and their altered expression level has been associated with many diseases and pathological disorders. Enzymes therefore can be exploited as a pristine biological trigger to tune material responses and to achieve controlled release of biomolecules at desired sites. This...
Article
The increasing demand for early detection of diseases drives the efforts to develop more and more sensitive techniques to detect biomarkers in extremely low concentrations. Electromagnetic modes at the surface of one dimensional photonic crystals, usually called Bloch surface waves, were demonstrated to enhance the resolution and constitute an attr...
Article
The layer-by-layer (LbL) technique is a simple and robust process for fabricating functional multilayer thin films. Here, we report the use of de novo designed polypeptides that self-assemble into coiled-coil structures (four-helix bundles) as a driving force for specific multilayer assembly. These pH- (sensitive between pH 4 and 7) and enzyme-resp...
Conference Paper
We describe the design and fabrication of biochips based on one dimensional photonic crystals supporting Bloch surface waves for label-free optical bio-sensing. The planar stacks of the biochips are composed of silica, tantala and titania that were deposited using plasma ion assisted evaporation under high vacuum conditions. The biochip surfaces we...
Article
We report the preparation of polymer capsules containing liposomal subcompartments, termed capsosomes, and their ability for the sustained delivery of protein therapeutics. Capsosomes were formed through the layer-by-layer (LbL) assembly of polymers and protein-loaded liposomes, followed by the formation of a capsule membrane based on disulfide cro...
Article
Full-text available
We describe the design and fabrication of biochips based on one dimensional photonic crystals supporting Bloch surface waves for label-free optical bio-sensing. The optical properties of Bloch surface waves are studied in relation to the geometry of the photonic crystals and on the properties of the dielectric materials used for the fabrication. Th...
Article
Full-text available
Polyvinylpyrrolidone (PVP) is a biocompatible, water-soluble polymer with unique physicochemical properties and attractive biological features that has found widespread use in several industries. Owing to advances in controlled polymerisation techniques, PVP can be easily synthesised with robust control over its architecture. However, the synthesis...
Article
Full-text available
Bloch surface waves (BSW) propagating at the boundary of truncated photonic crystals (1D-PC) have emerged as an attractive approach for label-free sensing in plasmon-like sensor configurations. Due to the very low losses in such dielectric thin film stacks, BSW feature very low angular resonance widths compared to the surface plasmon resonance (SPR...
Article
Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Rec...
Article
A highly sensitive label-free assay for the determination of blood coagulation Factor XIII activity is demonstrated through the controlled assembly of peptide-functionalized gold nanoparticles (AuNPs). Activated Factor XIII catalyzes the formation of covalent crosslinking between peptide chains through ε-(γ-glutamyl)-lysine bonds leading to the agg...
Article
We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on biomaterials were obtained by the formation of multicompositional, layered films via simple...
Article
Liposomes and polymersomes have attracted significant attention and have emerged as versatile materials for therapeutic delivery and in the design of artificial cells and organelles. Through the judicious choice of building blocks, these synthetic carriers can be readily engineered with tailored interfacial properties, offering new possibilities fo...