Rômulo Batista Rodrigues

• PhD Animal Science
• PostDoc Position at Federal University of Rio Grande do Sul

Fish sperm cryopreservation is an important technique for optimizing juvenile production in aquaculture stations and laboratories and contributing to the conservation of endangered species. Despite its benefits, the cryopreservation process can cause cellular damage, affecting spermatozoa quality and offspring viability. This study aimed to evaluat...

Aims: The use of anesthetics in fish ensures that they can undergo common handling in fish farming without causing damage to production. The aim of the present study was to determine the induction and recovery times of Piaractus mesopotamicus submitted to eugenol anesthesia. Study Design: 100 P. mesopotamicus were used, divided into two weight clas...

The agricultural production of the Argentine Pampa is based on intensive cultures, and the most widely used insecticides correspond to cypermethrin (pyrethroid) and chlorpyrifos (organophosphate), among others. Sperm motility and fertility assessments are essential topics for biology and population dynamics studies on fish species. In this study th...

Corpo do resumo: O sistema automatizado de ordenha (AMS) tem ganhado espaço nas propriedades brasileiras nos últimos anos. Os principais fatores que fazem os produtores adquirirem AMSs é a diminuição da mão de obra, gerenciamento de dados e aumento da produção de leite. Porém, os AMSs modificaram o comportamento das vacas durante seu ciclo de produ...

Introduction Electronarcosis is the most commonly used stunning method for large animals, but its consequences in tilapia still need to be evaluated. The aim of the study was to evaluate the application of electronarcosis in the pre-slaughter stunning of Nile tilapia (Oreochromis ni-loticus) and verify its effects on dynamic physiological balance a...

Phractocephalus hemioliopterus, Hemisorubim platyrhynchos, and Zungaro zungaro are native of South American rivers whose population is being threatened by the damming of rivers to build hydroelectric dams, fishing pressure and environmental degradation. Basic information on these species' reproductive characteristics is necessary to enable producti...

This study aimed to evaluate the viability of reproductive gametes in zebrafish ( Danio rerio ), at different rigor mortis stages. Viability assessments were conducted on oocytes at various developmental stages using LIVE/DEAD and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. For sperm evaluation, both kine...

Southern black drum ( Pogonias courbina ) is a species distributed along the western Atlantic Ocean, and it is the largest Sciaenidae observed in the coast of Rio Grande do Sul state, Brazil. However, it is listed as a vulnerable species at The IUCN Red List of Threatened Species™, and their fishing is prohibited. The objective of this study was to...

In brief The containers used in cell cryopreservation are essential to maintain cell integrity and viability after thawing. This paper reveals the methodology of using biodegradable containers for fish sperm cryopreservation. Cryopreserved sperm in biodegradable containers showed high fertility capability. Biodegradable capsules could be alternativ...

Free article at https://authors.elsevier.com/c/1h90CACeYFYnR until 15/06/23.

INTRODUÇÃO Rã-Touro (Lithobates catesbeianus) é uma espécie explorada a nível mundial por sua facilidade de cultivo e excelente qualidade da carne [1]. Os lipídeos for-necem altos valores de energia bruta e ácidos graxos para os animais[2, 3]. Os ácidos graxos são necessários para o crescimento, reprodução e ainda melhora a conversão alimentar para...

This systematic review provides an overview of the history and current status of cryopreservation of fish sperm and a detailed evaluation of cryoprotocols using powdered milk. A literature search was performed in PubMed, Scopus, Web of Science, and SciELO databases. Twenty-nine articles were selected after excluding duplicate articles or articles t...

Zebrafish is an important animal model, thousands lines have been developed, thus having a great need for their preservation. However, the cryopreservation of fish oocytes is still limited and needs improvement. The sodium alginate hydrogel, in addition to providing support for the cells, has been shown to be a potential cryoprotectant. Therefore,...

In this study, we assessed the effect of temperature on gonadal development and sex ratio in Brycon orbignyanus. The fish were kept at three different temperatures (20 °C, 23 °C, and 26 °C) from the beginning of exogenous feeding. At 420 days after fertilization, 10 individuals were collected per temperature regimen for histological and hormonal ev...

Brycon hilarii is a neotropical fish whose population in rivers is being threatened by overfishing, construction of hydroelectric dams, transposition of riverbeds, and pollution. Fundamental knowledge of the reproductive characteristics of this species is necessary to enable its breeding in captivity, for aquaculture as well as for biological conse...

This study suggests that pirarucu is a fish capable to have competitive products in the national premium fish market and analyze the opportunities and constraints of this supply chain in Brazil. To achieve that, a semi-structured interview was conducted with 22 players of the cultivated pirarucu supply chain in Brazil, resulting in more than 13 hou...

The aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg-1 ; pH 7.6; 24°C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at-170°C for 18h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% met...

This study aimed to analyze the sperm quality of Colossoma macropomum, during the reproductive period. A total of 23 males of C. macropomum in the breeding season were used. Male gametes were collected after the hormone induction protocol using volumetric syringes for quantitative and qualitative analyses throughout the reproductive season, the fis...

The use of technologies in assisted reproduction, such as the use of centrifugation, has been reported as a tool that allows separating the best portion of sperm from cryopreserved milt. The main of this work was to compare two separation techniques, differential and density gradient, on thawed Rhamdia quelen milt, based on sperm functionality and...

Although Brycon orbignyanus has traditionally been extensively fished in the Paraná and Uruguay basins, anthropic events and fishing pressure have substantially diminished natural stocks. Restocking programmes have, nevertheless, aided efforts to restore the stocks of this fish in its former ranges. The objective of this study was to characterize t...

This study aim was to verify whether milt quality of male Leiarius marmoratus is maintained among successive samples collected during the same reproductive period. Ten reproductively mature males were used to evaluate four successive sperm samples collected at 10-day intervals. For these collections, seven males were injected with carp pituitary ho...

The aim of this study was to analyze the physicochemical and microbiological characteristics of the water used in fish processing and tilapia fillets in slaughterhouses. The study analyzed the processing water from nine slaughterhouses. The water samples for analysis were collected at three points: process water (PW), it is the water used inside th...

Piracanjunba (Brycon orbignyanus) is an endangered South American fish, and ovarian tissue cryopreservation is an alternative method for preserving maternal germplasm and genetic diversity. Therefore, our aim was to test a vitrification protocol for ovarian tissue containing primary growth (PG) oocytes of B. orbignyanus as a strategy to avoid the t...

Contamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen male...

Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm. The proposed method seeks to reduce the presence of cryoprotectant in the cryopreserved milt of the Rhamdia qh...

Although gamete cryopreservation has facilitated advancement of reproduction research by allowing the storage of cells over prolonged periods of time, during freezing-thawing cycles, cells inevitably suffer from cryoinjuries. Here, we evaluate oxidative stress and DNA damage of zebrafish sperm at different stages of the cryopreservation process. It...

The aim of this study was to investigate the effects of different concentrations of glutamine (experiment 1) and glutamine + cysteine (experiment 2) on post-thaw sperm quality of South American silver catfish (Rhamdia quelen). The sperm was cryopreserved in cryoprotectant solution (fructose 50 g/L, powdered milk 50 g/L and methanol 100 ml/L) contai...

Anesthesia is a common practice used in fish research and aquaculture. It is important to understand anesthetic effects on the animal and tissues of interest to ensure validity of data and to improve animal welfare in research and fish production endeavors. The production of some captive fish species is only possible by imposing artificial reproduc...

Cryoprotectants play a vital role in the cryopreservation process, protecting biological samples from freezing damage. Here, we evaluate the effects of the combination and interaction of different extenders with permeable and non-permeable cryoprotectants, on the cryopreservation of Danio rerio sperm, analyzing the effects of cryopreservation throu...

The present study aimed to evaluate the morphometric and yield characteristics and the chemical composition of Pimelodus britskii in two seasons (winter or summer) to provide information for its industrialization and commercial use. For this trial, 72 individuals were collected from either winter or summer. The fish were distributed to three length...

For fish farming, water is the limiting factor for good productivity. Limnological characteristics determine the health and the productivity of the fish and can indicate possible problems arising in the ecosystem. The present study aimed to verify the nictemeral and vertical dynamics of limnological characteristics in a tilapia farming tank coated...

Technologies in the industrialization of fish are being increasingly exploited; as a result, the aquaculture sector has increased the number of residues derived from the fillet, increasing in this way the interest in research on the use of this waste. Therefore, researches are being carried out aiming to characterize the residues according to their...

The aim of the present study was to evaluate the effect of cryopreservation on the morphology of zebrafish sperm (Danio rerio). Sperm from 30 males were collected and divided in two treatments: fresh and cryopreserved semen. The following were measured sperm morphology, motility and membrane integrity. Cryopreservation reduced motility, the number...

The objective of the study was to determine the physical-chemical composition of protein hydrolysates from meat by-products from poultry and swine processing, as well as to evaluate the apparent digestibility coefficients (ADC) of crude protein (ADCCB), crude energy (ADCCE), essential (ADCEAA), and non-essential (ADCNEAA) amino acids for juvenile N...

O pólen apícola é um produto natural constituído de vários nutrientes, rico em aminoácidos livres e com baixo teor de gordura, além de propriedades profiláticas e terapêuticas, enquanto que a própolis é um produto natural utilizado de diversas formas como apiterápico na medicina popular e agente anti-inflamatório. Pesquisas estão sendo realizadas v...

The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the best cryoprotectant solution for each cryopreserv...

Anesthesia is a common practice used in fish research and aquaculture. For both applications, it is important to understand anesthetics effects on the animal and tissues of interest to ensure the validity of data and to improve animal welfare. Captive fish production is only possible with artificial reproduction, and it is known that manipulation i...

The aim of this study was to evaluate different levels of soybean oil inclusion in diets for Nile tilapia. 270 tilapia, with an initial weight of 425.33 ± 32.37 g and initial length of 25.53 ± 2.00 cm, were randomly distributed in 15 tanks. The diets were formulated to be isonitrogenous (30%) using five increasing levels of soybean oil (0.00; 15.00...

Valine belongs to the group of branched-chain amino acids, has an important structural role and is primarily deposited as body protein. The present study evaluated the effects of valine in diets of juvenile Nile tilapia. A total of 216 juveniles with weight of 21.40 ± 0.42 g and length of 10.07 ± 1.00 cm were distributed into 18 aquarium in a compl...

The cryopreservation protocols are species‐specific owing to variable sperm sensitivity towards temperature reduction and contact with cryoprotectant solutions. However, little is known about spermatic pathologies, especially after the cryopreservation process. Thus, the objective was to evaluate the effect of cryopreservation on morphological chan...

Many microorganisms' potential remains unexploited due to the current inability to preserve them at an industrial scale. Lactic acid bacteria (LAB), are a group of microorganisms widely used for producing a wide diversity of fermented foods. The market of concentrated cultures (starters) is continuously growing due to the development of health bene...

The production of captive fish is only possible through artificial reproduction, but manipulation is a known stressor stimulus. Thus, the objective of the present study was to evaluate the effects of different eugenol concentrations (0, 30, 40, 50 and 60 mg/L) during reproductive management of Rhamdia quelen. Seventy-five mature male R. quelen were...

RESUMO: O pólen apícola é um produto natural constituído por vários nutrientes, rico em aminoácidos livres e com baixo teor de gordura, além de propriedades profiláticas e terapêuticas, desta maneira é uma excelente opção na nutrição animal e pode oferecer benefícios ao desempenho e saúde dos animais de produção. Este trabalho foi realizado para av...

The golden mussel (Limnoperna fortunei) possesses high fouling potential and has caused damage to various productive sectors. These individuals do not present known natural enemies, but they may be part of the feeding of one or more species of fish, which would help in their control. The main species of fish cultivated in cages in the Itaipu reserv...

ABSTRACT This study verified the effect of valine on the zootechnical performance, percentage composition and growth of skeletal muscle fibre in fingerlings of Nile tilapia. A total of 270 fish (average initial weight 1.57 ± 0.05 g and total initial length 4.16 ± 0.46 cm) were used, distributed in 18 tanks (250 litres), in a completely randomised d...

Vitrification of ovarian tissue containing immature oocytes provides an important tool for protecting the endangered species and genetic diversity in aquatic species. Therefore, the main objective was to assess primary growth (PG) oocytes viability following ovarian tissue vitrification using histological analysis, two staining protocols (trypan bl...

Resumo: O estudo foi realizado com o objetivo de avaliar a farinha de ossos de peixe em substituição ao fosfato bicálcico em dietas para pós-larvas de tilápia do Nilo. Foram utilizadas 400 pós-larvas de tilápia do Nilo com peso médio inicial de 0,03 g distribuídas em 20 aquários com volume de 30 L e alimentadas com as dietas experimentais por 26 di...

Resumo O avanço da aqüicultura e a crescente demanda por alimentos de origem animal, aliado à preocupação com a segurança alimentar dos consumidores, vêm fazendo com que se aumentem os cuidados com a produção animal, contaminação do ambiente e suas complicações. Com isso, esta revisão foi realizada com o objetivo de debater os principais fundamento...

A farinha de peixe é tradicionalmente o alimento de origem animal mais utilizado como fonte proteica na nutrição de peixes. Contudo, a estabilização da produção pesqueira re-sulta na diminuição da produção de farinha de peixes, tornando necessária a utilização de fontes proteicas alternativas. Dentre as alternativas, tem-se utilizado os alimentos d...

Este trabalho foi realizado com o objetivo de determinar a digestibilidade aparente da proteína bruta, extrato etéreo, energia bruta, cálcio e fósforo das farinhas de carne e ossos de tilápia do Nilo (Oreochromis niloticus), do catfish (Ictalurus punctatus), do pintado (Pseudoplatystoma corruscans) e do bagre africano (Clarias gariepinus) para a ti...

This paper was aimed to determine the apparent digestibility of crude protein, crude fat, gross energy, calcium and phosphorus of tilapia meat and bone meal (Oreochromis niloticus) (TMBM), the catfish meat and bone meal (Ictalurus punctatus) (CMBM), pintado cathfish meat and bone meal (Pseudoplatystoma corruscans) (PCMBM) and the African catfish me...

The piava (Leporinus obtusidens) is an omnivorous native fish of southeastern and southern Brazil and has great potential for national fish farming. In this study, were evaluated growth, digestive enzyme activity and body composition of piavas fed diets containing fruit waste: grape, orange, guava and fig. They were used 180 fish (initial weight =...

El avance de la acuicultura y la creciente demanda de alimentos de origen animal, junto con la preocupación por la seguridad alimentaria de los consumidores, están ocasionando que aumente la atención de la producción ganadera, la contaminación ambiental y sus complicaciones. Por lo tanto, esta revisión se llevó a cabo para discutir los fundamentos...

A piava (Leporinus obtusidens) é um peixe nativo das regiões sudeste e sul do Brasil, de hábito alimentar oní­­voro e com grande potencial para a piscicultura nacional. Neste estudo foi avaliado o crescimento, atividade de enzimas digestivas e composição corporal de piavas alimentadas com dietas contendo resí­­duos de frutas: uva, laranja, goiaba e...

RESUMO Dentre os sistemas de cultivo utilizados na aquicultura, os sistemas com uso limitado de água são alternativas para o desenvolvimento sustentável do setor produtivo, em especial em locais com baixa disponibilidade de água. O sistema de cultivo em bioflocos é uma alternativa de criação em sistema fechado, que vem mostrando resultados positivo...