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April 2014 - October 2020
Publications
Publications (50)
Diffuse large B-cell lymphoma (DLBCL) is a malignancy of immense biological and clinical heterogeneity. Based on the transcriptomic or genomic approach, several different classification schemes have evolved over the years to subdivide DLBCL into clinically (prognostically) relevant subsets, but each leaves unclassified samples. Herein, we outline t...
Simple Summary
Diffuse large B-cell lymphoma (DLBCL) is the predominant type of non-Hodgkin lymphoma—the most common haematological malignancy worldwide. These two cancers are cancers of the immune system and dyslipidemia is a hallmark of both cancer and inflammation. Cancer needs specific lipid species to sustain growth and survival. The global pl...
We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co⁶⁰ at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological s...
Herein we present the potential of four machine learning (ML) algorithms: Partial Least Squares – Discriminant Analysis (PLS-DA), Random Forests (RF), Support Vector Machines (SVM) and Decision Trees (DT) to classify human plasma samples into cancer or control group, using a lipidomic dataset retrieved from the research article „Lipidomic profiling...
Structural changes of glycans are observed in different (patho)physiological conditions. Human placental membrane (glyco)proteins were isolated from the first and third trimester placentas of mothers at different ages. By using lectin microarray, we demonstrated that the placental membrane N-glycome contains several N-glycan groups: high mannose, a...
Glycosylation of cell receptors influences their function and development of tumour induces changes in glycosylation. Cell growth depends on the activation of receptors which bind growth factors and the insulin-like growth factor (IGF) receptors are among the most important ones. Usually, only small quantities of isolated receptors are available th...
The two new heterometallic Ru(II)-tpy/ferrocene complexes [Ru(tpy)Cl2(mtefc)] (1) and [Ru(tpy)Cl2(mtpfc)] (2) (where tpy = 2,2′:6′,2′′-terpyridine, mtefc = (2-(methylthio)ethyl)ferrocene, and mtpfc = (3-(methylthio)propyl)ferrocene) have been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV–vi...
With the aim of assessing whether Au(III) compounds with pincer type ligands might be utilized as potential antitumor agents, three new monofunctional Au(III) complexes of the general formula [Au(N-N'-N)Cl]Cl2, where N-N'-N = 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine (H2LtBu, 1), 2,6-bis(5-tert-butyl-1-methyl-1H-pyrazol-3-yl)pyridine (Me2LtBu,...
Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individu...
Three coordination compounds of ruthenium(II), belonging to a recently synthesised series of water-soluble compounds of general formula mer-[Ru(L3)(N-N)Cl]Cl, where L3 = 4'-chloro-2,2':6',2''-terpyridine (Cl-tpy), N-N = ethylenediamine (en), 1,2-diaminocyclohexane (dach) or 2,2'-bipyridine (bpy), have shown strong binding to calf thymus DNA and mod...
Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques...
Introduction:
Insulin receptor (IR) and type 1 and type 2 insulin-like growth factor receptors (IGF1R and IGF2R) play important roles in regulation of placental and foetal growth. All three receptors are abundantly glycosylated. N-glycosylation significantly affects protein conformation and may alter its function. We have recently found that the N...
Placental insulin receptor (IR) and insulin-like growth factor receptors (IGFRs) are essential for fetal growth. We investigated structural changes of these receptors exposed to increased oxidative stress in mothers diagnosed with diabetes mellitus (DM) or preeclampsia (PE) complicated with intrauterine growth restriction. Increased amount of IR an...
Alterations in the glycosylation of few membrane proteins from human placenta during gestation have been documented, but data on N-glycome of placental membrane proteins is still missing. The primary goal of this study was to obtain N-glycan profiles of human placental membrane proteins using a reliable, simple and high-throughput method. The secon...
Structural and ligand-binding properties of the insulin-like growth factor-binding protein (IGFBP)-3 in patients with poorly controlled diabetes mellitus type 2 were investigated using boronic acid- and lectin-affinity chromatography. IGFBP-3 species separated by chromatography were analyzed by immunoblotting and surface-enhanced laser desorption/i...
N-Glycosylation Pattern of Human Placental Insulin-Like Growth Factor and Insulin Receptors in Well-Controlled Pregestational Diabetes Mellitus
Diabetes mellitus is a complex disease that leads to alterations in the glycosylation of proteins. Insulin-like growth factor and insulin receptors are involved in the regulation of fetal and placental grow...
Placenta is a source of carbohydrate-binding proteins that function as molecular scavengers, but they could also be involved in interactions that assist in metabolic control. Mannose/N-acetyl-glucosamine (Man/GlcNAc)-binding proteins from placenta were isolated and their reactivity towards placental insulin and insulin-like growth factor receptors...
The aim of this study was to examine changes in some hormones concentrations in calves during the first 32 hours of neonatal life and to estimate their association with glycemia. Thyrty two Holstein breed calves were selected for the study. Blood samples were taken at 30, 60 and 90 minutes postnatal. Calves received pooled colostrum: primary colost...
Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. Th is is mainly because antibodies specifi cally raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate...
Little is known on the possible association between impaired glucose/insulin metabolism, the pattern of IGFBP-1 phosphorylation and the complex formation with other serum proteins. In this study, the concentration, isoform, multimer and complex pattern of IGFBP-1 was compared in healthy persons and patients with type 2 diabetes mellitus or with hyp...
The insulin-like growth factor-binding protein 1 (IGFBP-1) is a member of a family of six homologous proteins that regulate the action of the insulin-like growth factors. IGFBP-1 is a 25 kDa protein that, in addition to its native form, may exist in several phosphoforms (30 kDa), which are predominant in the circulation of humans. Phosphorylation o...
Insulin-like growth factor binding protein-3 (IGFBP-3) is an important modulator of development and progression of breast cancer as it regulates the amount of free, physiologically active IGF-I and IGF-II. Changes in the glycosylation pattern within IGFBP-3 may affect its interaction with ligands. The aim of this study was to investigate whether su...
Multiple insulin-like growth factor binding proteins (IGFBPs) are found in sera of various species. This study was conducted to investigate whether IGFBPs in porcine serum could be detected using antibodies and antisera raised against human IGFBP-1, -2, -3 and -4. Western ligand blot procedure was used for the identification of c in porcine serum....
Human IGFBP-3 contains three potential N-linked glycosylation sites. Published data concerning the type and saccharide composition of the N-glycans is scarce. The aim of this study was to characterise N-glycans covalently attached to IGFBP-3 from sera of healthy adults (men and women). In order to do that a panel of eight lectins covering broad sac...
Fetal growth in utero depends on the proper development and function of the placenta. Insulin-like growth factors (IGFs) are critically involved in placental development. During pregnancy, an IGF-binding protein, IGFBP-1, which is produced by maternal decidua, plays an important role in the control of the bioavailability of IGFs. It has recently be...
Association of IGFBP-1, IGFBP-2 and IGFBP-3 with other proteins in human serum and placental cell membranes was investigated using affinity chromatography matrix with immobilized antibodies. Circulating IGFBP-1 was found to be predominantly bound to alpha(2)-macroglobulin and not in the binary complex with its ligand, IGFBP-2 complexes and/or polym...
Insulin and insulin-like growth factors (IGFs) bind to their cognate receptors with high affinities, but due to their homology they may cross-react with each other's receptors. We performed a series of binding studies to reanalyze the cross-reactivity of insulin, IGF-I, and IGF-II to affinity-purified insulin (IR) and type 2 IGF receptors (IGF-2R)...
In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three...
The ability of plant lectins to modify the interactions of the insulin receptor (IR) and insulin-like growth factor (IGF)
receptors (IGFRs) with their ligands was investigated. The lectins profoundly affected the competition-binding curves for
125I-labelled IGF-I and insulin, causing an increase in the affinity of placental IGF1R and IR towards the...
There is a tight connection between insulin-like growth factor binding protein 1 (IGFBP-1) and nutrient/energy supply, suggesting modulation of the short-term insulin-like activity and glucose homeostasis by IGFBP-1. Differential phosphorylation of IGFBP-1 alters its affinity for insulin-like growth factors (IGFs) and its capacity to modulate cellu...
There is disagreement in the literature about the ability of neonatal calves to absorb perorally administered insulin. This study evaluated the absorption of a bolus of insulin administered alone or with an energy souce and its effects on the circulating insulin-like growth factor system and thyroid hormones in newborn Holstein-Friesian calves. Wit...
Insulin and insulin-like growth factor receptors (IR, IGF-IR, IGF-IIR) from human placental cell membranes were solubilised and their glycoprotein properties were studied in terms of their interaction with five lectins: wheat germ agglutinin (WGA), banana lectin (BanLec), phytohaemagglutinin (PHA), concanavalin A (Con A), and Sambucus nigra aggluti...
Serial measurements of blood glucose concentration before and after giving a specific amount of glucose orally provide a standard method to evaluate glucose metabolism. Although the reference ranges for glucose concentration in various disease states that are based on impaired glucose homeostasis have been established, the reference values are not...
Izvod Ura|en je ogled prema protokolu hormonalne sinhronizacije estrusa i stimulacije razvojàutog tela pomo}u gonadorilizing hormona GnRH i prostaglandina PGF2á na 18 krava hol{tajn-frizijske rase, 50-70 dana posle teljenja. Klini~ki je odre|ivana veli~ina ja-jnika i merena koncentracija progesterona u uzorcima mleka EIA postupkom i u uzorci-ma krv...
The biological activities of IGF-I and II are mediated mainly by the type 1 IGF receptor (IGF 1R) and controlled by their interaction with soluble proteins, the IGF binding proteins (IGFBPs). Although there is a growing body of evidence that some IGFBPs may be cell surface-bound, published data concerning cell association of IGFBP-1 are scarce and...
The purpose of this investigation was to compare peripheral circulating concentrations of the insulin-like growth factors (IGF) and thyroid hormones (T3 and T4) during the periparturient period in dairy cows in different situations. In Study 1 from an average of about 4 days prepartum to 4 days postpartum the decreases in serum T3, T4 and IGF-I wer...
The protein synthetic activity of the liver is diminished in cirrhosis. The aim of this study was to investigate possible changes in the serum IGF-IGFBP system among patients with alcoholic liver cirrhosis (ALC). The results obtained demonstrated that serum IGF-I and IGF-II concentrations were significantly lower in patients with ALC than in health...
The aim of this investigation was to determine the total concentrations of the insulin-like growth factors (IGF-I and IGF-II) in the blood serum of patients with liver cirrhosis and to evaluate their association with the condition. Cirrhosis was alcohol induced (n=27), of viral origin (n=17) or due to combined or other causes (n=21) and was moderat...
Bovine milk is an important ingredient in human nutrition as well as in the diet of the young calf. In view of the role of insulin-like growth factors (IGFs) in growth and development attempts were made to determine the concentrations of both IGF-I and IGF-II in colostrum and milk. Colostrum was collected on the day of calving from six dairy cows o...
Insulin-like growth factor-II (IGF-II) is a polypeptide mitogen present at relatively high concentrations in the human peripheral circulation. The aim of the present investigation was to develop a reliable assay system for determining total IGF-Il, for use in studies of the IGF system. A competitive binding assay was set up using a specific monoclo...
Serum insulin-like growth factor-I (IGF-I) was determined by radioimmunoassay at different periods of the menstrual cycle in order to detect possible alterations associated with the regular changes in gonadotropin and steroid hormone concentrations. In the first study (n=19) no differences were found between the values on days 7, 14 and 21 in the t...
