Roman Kostyuchenko

Roman Kostyuchenko
Saint Petersburg State University | SPBU · Department of Embryology

PhD

About

42
Publications
13,823
Reads
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608
Citations
Introduction
Roman Kostyuchenko currently works at the Department of Embryology, Saint Petersburg State University. Roman does research in Developmental Biology. Their current projects are 'Spiralia: ooplasmic segregation, specification of cell lineages, and establishment of body plan in development' and 'Cellular and molecular mechanisms of regeneration and asexual reproduction in annelids'.
Additional affiliations
September 2006 - November 2006
European Molecular Biology Laboratory
Position
  • Scientific Collaborator
October 1999 - present
Saint Petersburg State University
Position
  • Professor (Associate)
Education
May 1999 - May 1999
Saint Petersburg State University
Field of study
  • Developmental biology, Embryology

Publications

Publications (42)
Article
Full-text available
Nanos is a translational regulator that is involved in germline development in a number of diverse animals and is also involved in somatic patterning in several model organisms, including insects. Neither germline development nor somatic stem cell lines/undifferentiated multipotent cells have been characterized in the development of the annelid Ali...
Article
Full-text available
The question of why animals vary in their ability to regenerate remains one of the most intriguing questions in biology. Annelids are a large and diverse phylum, many members of which are capable of extensive regeneration such as regrowth of a complete head or tail and whole-body regeneration, even from few segments. On the other hand, some represe...
Article
Full-text available
Epimorphic regeneration of lost body segments is a widespread phenomenon across annelids. However, the molecular inducers of the cell sources for this reparative morphogenesis have not been identified. In this study, we focused on the role of fibroblast growth factor (FGF) signaling in the posterior regeneration of Alitta virens. For the first time...
Article
Full-text available
Проведен сравнительный анализ событий морфаллаксиса и эпиморфоза при регенерации и бесполом размножении у аннелид. Показаны перестройки на молекулярном, клеточном и системном уровнях уже на самых ранних этапах восстановительных морфогенезов. Отмечена принципиальная необходимость морфаллаксиса для инициации, прогресса и завершения событий по типу эп...
Article
Full-text available
Недавние работы показали, что, несмотря на стереотипное дробление, аннелиды демонстрируют способность к эмбриональным регуляциям, в том числе при формировании линии половых клеток. Вместе с тем наиболее широко процессы регуляции представлены в ходе постларвального развития аннелид. Способность к регенерации, являющейся, вероятно, анцестральным приз...
Article
Full-text available
This article addresses the question of the role of morphallactic and epimorphic events in regeneration and asexual reproduction in annelids. It was shown that tissue reorganization and changes at the molecular level occur already at the very early stages of regenerative processes. These data indicate that morphallaxis is required for all progressiv...
Article
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Recent studies have shown that, despite stereotypical cleavage, annelids show the ability for embryonic regulation, including the formation of germline cells. However, the widest variety of regulative processes is presented in the postlarval development of annelids. The ability to regenerate, which is probably an ancestral feature, manifests itself...
Article
Full-text available
In this paper, the role of the Wnt/β-catenin pathway in the establishment of the body plan in multicellular animals has been analyzed. We demonstrated that at different stages of development the Wnt signaling cascade is involved in several directly unrelated processes such as inner germ layer specification, anterior–posterior axis patterning, and t...
Article
Full-text available
Kisspeptins (KPs) and their receptor (KISS1R) play an important role in many physiological processes in the body, such as sexual maturation, reproductive system functioning, placentation, insulin secretion, and vasoconstriction. The highest level of kisspeptins and their receptor is observed in the organs of hypothalamic-pituitary-gonadal and hypot...
Article
Full-text available
Regenerating segments in polychaetes offer a vivid example of epimorphic recovery of the lost organs and tissues. It is also a promising object for studying positional information and the mechanisms maintaining the body integrity. With the aim to develop a convenient standardized model, we described the dynamics of recovery of the major anatomical...
Article
Full-text available
The ability of regulation, including restoration of the missing parts, to normalize the development, is a fundamental characteristic of living systems. However, until recently, representatives of the group Spiralia (animals with spiralian development) have been considered a typical example of determinative development. Meanwhile, studies in recent...
Article
Full-text available
Петербургская эмбриологическая школа зародилась еще в 18–19 веке и известна работами К.Ф. Вольфа и К. Бэра, А.О. Ковалевского, И.И. Мечникова и многих других. Однако как структурная единица Санкт-Петербургского (Петроградского–Ленинградского) университета она появилась лишь в начале 20-х годов 20 столетия, когда была сформирована лаборатории эмбрио...
Article
Full-text available
A comparative study of regeneration and asexual reproduction across annelids is presented. The importance and universality of early stages of the restoration morphogeneses, which, however, vary in details, is shown. Here, we concern on the conserved elements of genetic regulatory programs involved in both processes, responsible for cell fate plasti...
Article
Full-text available
An analysis of the comparative-morphological, molecular-genetic, and evolutionary aspects of the teloblastic mesoderm formation in the Spiralia representatives was performed. The conservative and the most expressive varying features of morphogenesis and genetic developmental programs of the mesodermal germ layer were considered. Using nereid polych...
Article
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Mesoderm represents the evolutionary youngest germ layer and forms numerous novel tissues in bilaterian animals. Despite the established conservation of the gene regulatory networks that drive mesoderm differentiation (e.g. myogenesis), mechanisms of mesoderm specification are highly variable in distant model species. Thus, broader phylogenetic sam...
Article
Full-text available
Polychaetes are famous for their outstanding ability to regenerate lost body parts. Moreover, these worms possess a number of ancestral features in anatomy, development, and genetics, making them particularly suitable for comparative studies. Thus, fundamental as well as new undisclosed so far features of regenerative processes may be revealed, usi...
Article
Full-text available
The analysis of comparative-embryological and molecular-biological data leads to the conclusion that universal basic mechanisms of morphogenesis occurred first in the evolution of animals in the ancestors of modern sponges and eumetazoans, which served as a basis of different evolution of individual development in Parazoa and Eumetazoa lines. In th...
Article
Full-text available
In spiralians, the specification of cell lines in development is provided by maternal factors. However, recent studies demonstrated the importance of inductive processes whose significant element is cellular signaling. Our data argue the conditional specification of a number of cell lines at the early stages of development of the mollusk Testudinal...
Article
Full-text available
SUMMARY Annelids and arthropods, despite their distinct classification as Lophotrochozoa and Ecdysozoa, present a morphologically similar, segmented body plan. To elucidate the evolution of segmentation and, ultimately, to align segments across remote phyla, we undertook a refined expression analysis to precisely register the expression of conserve...
Data
Supplementary methods. Steinmetz_SupplMethods.xls is an Excel Spreadsheet containing primer sequences, template source and PCR programs used to clone six3 and otx genes presented in the paper.
Data
Supplementary figures and figure legends. Steinmetz_Suppl_Figs.pdf contains two supplementary figures and legends showing multiple sequence alignments of six3 and otx genes, and supporting whole mount in situ hybridisation data of Platynereis, Strigamia, and Euperipatoides larva.
Article
Full-text available
The heads of annelids (earthworms, polychaetes, and others) and arthropods (insects, myriapods, spiders, and others) and the arthropod-related onychophorans (velvet worms) show similar brain architecture and for this reason have long been considered homologous. However, this view is challenged by the 'new phylogeny' placing arthropods and annelids...
Article
Full-text available
The bilaterian animals are divided into three great branches: the Deuterostomia, Ecdysozoa, and Lophotrochozoa. The evolution of developmental mechanisms is less studied in the Lophotrochozoa than in the other two clades. We have studied the expression of Hox genes during larval development of two lophotrochozoans, the polychaete annelids Nereis vi...
Article
Full-text available
Oligochaetes Nais communis and Pristina longiseta are capable of paratomy, i.e., asexual reproduction of the cross division type, when division proceeds across the long body axis and daughter organisms retain the maternal axes. Paratomy is represented by two forms: slow and rapid. Slow paratomy is accompanied by the formation of chains from no more...
Article
Full-text available
Prototroch formation was studied in the polychaete Nereis virens using light, scanning electron, and confocal laser microscopy. Cell lineage of trochoblasts was followed and chronology of their appearance was determined. The prototroch ciliary ring is formed by twelve descendants of micromere 1m 2. The remaining four primary trochoblasts have no ci...
Article
Full-text available
We have studied the posterior Hox gene Nvi-Post1 expression in the early development of the polychaete Nereis virens. This is the first evidence of the posterior group Hox genes expression during the larval development of a Lophotrochozoan. The expression begins in the trochophore hyposphere at the prospective sites of larval parapodia. As the larv...
Article
Full-text available
Ooplasmic segregation is of great importance in the development of Annelida. The mechanisms of this process are very diverse in different groups of polychaetes, oligochaetes, and leeches (Fernandezet al., 1998). Ooplasmic segregation in Nereis virens is connected with the first meiotic spindle formation and animal-vegetative axis appearance. Spheri...
Article
Full-text available
Ooplasmic segregation is of great importance in the development of Annelida. The mechanisms of this process are very diverse in different groups of polychaetes, oligochaetes, and leeches (Fernandez et al., 1998). Ooplasmic segregation in Nereis virens is connected with the first meiotic spindle formation and animal-vegetative axis appearance. Spher...
Article
Full-text available
The ooplasmic segregation in the Nereis virens egg starts after completion of cortical reaction. During ooplasmic segregation the radial symmetrical pattern of the oocyte transforms into polarized stratified structure with different kinds of cytoplasm regularly distributed along the animal-vegetal axis. The beginning of oocyte polarization is assoc...
Article
The differentiation of trochoblasts in Nereis virens and Platynereis dumerlii embryos was investigated during different periods of time after fertilization by means of the DNA replication block. It was shown that the quantal cycle of ciliogenesis is not connected directly with the time of primary trochoblast founder cell formation. Development of t...
Article
Full-text available
The differentiation of trochoblasts in Nereis virens and Platynereis dumerilii embryos was investigated during different periods of time after fertilization by means of the DNA replication block. It was shown that the quantal cycle of ciliogenesis is not connected directly with the time of primary trochoblast founder cell formation. Development of...
Article
We have studied the posterior Hox gene Nvi-Post1 expression in the early development of the polychaete Nereis virens. This is the first evidence of the posterior group Hox genes expression during the larval development of a Lophotrochozoan. The expression begins in the trochophore hyposphere at the prospective sites of larval parapodia. As the larv...

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Projects

Projects (2)
Project
Regeneration and asexual reproduction in annelids are similar (but not identical!) processes of post-embryonic development in which entire body parts are formed de novo. This fascinating phenomenon of natural reactivation of developmental programs in adulthood is of great importance for fundamental and applied biomedical sciences. We are focused on molecular profiling, tissue-reorganizing morphogeneses, cellular origin of blastema, and organogeneses, involved in establishment of the lacking segments. Using phylogenetically distant species of nereidid polychaetes, naidid and enchytraeid oligochaetes, our investigations indicate profound variability of developmental mechanisms leading to the same outcome.
Project
Spiral development, being common for many Lophotrochozoans, is considered as highly determinative (mosaic) and invariant. However, its driving forces (ooplasmic determinants, signal-inducing and mediating molecules, cytoskeleton-orchestrating mechanisms) remain to be enigmatic. Working on annelid and mollusk embryos we aim to unravel conservative and specialized traits of their developmental programs. Our research utilizes complex descriptive and experimental methods, and comparative approaches on several model organisms, allowing wide-range speculations and interpretations of the evolutionary events, which gave rise to remarkable biodiversity within Spiralia.