Romain Larbat

Romain Larbat
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Department of Environment and Agronomy

About

55
Publications
21,642
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2,090
Citations
Citations since 2017
26 Research Items
1447 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
Introduction
Now developping a new research project on PRI x climate condition for apple protection

Publications

Publications (55)
Article
Full-text available
Resistance to conventional treatments renders urgent the discovery of new therapeutic molecules. Plant specialized metabolites such as phenolamides, a subclass of phenolic compounds, whose accumulation in tomato plants is mediated by the biotic and abiotic environment, constitute a source of natural molecules endowed with potential antioxidant, ant...
Preprint
Full-text available
Phenolamides are specialized metabolites widely distributed in the plant kingdom. Their structure is composed by the association of hydroxycinnamic acid derivatives to mono-/poly-amine. This association is catalyzed by N-hydroxycinnamoyltransferases enzymes. Tomato plants are accumulating putrescine-derived phenolamides in their vegetative parts. R...
Article
Full-text available
Tomato plants are attacked by a variety of herbivore pests and among them, the leafminer Tuta absoluta, which is currently a major threat to global tomato production. Although the commercial tomato is susceptible to T. absoluta attacks, a better understanding of the defensive plant responses to this pest will help in defining plant resistance trait...
Article
Plants developed a series of defence mechanisms to counteract the attack of herbi-vores. These can impact on food-webs at various trophic levels, in both natural and managed ecosystems, such as crops. The biochemical and ecological bases behind these processes are reviewed here by highlighting the differences in direct and indirect , constitutive a...
Article
Full-text available
During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For this, leaves were collected at different position...
Article
Furanocoumarins are phytoalexins often cited as an example to illustrate the arms race between plants and herbivorous insects. They are distributed in a limited number of phylogenetically distant plant lineages, but synthesized through a similar pathway, which raised the question of a unique or multiple emergence in higher plants. The furanocoumari...
Article
Full-text available
Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems...
Preprint
Full-text available
Phagnalon saxatile subsp. saxatileest une espèce sauvage très répandue en Algérie qui est utilisée à des fins médicinales comme analgésique et anticholestérolémique. Cependant, les informations sur son contenu phytochimique sont encore rares. L'objectif de cette étude était d'identifier et de quantifier les composés phénoliques de différents extrai...
Preprint
Full-text available
Phagnalon saxatile subsp. saxatile is a wild species very widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, informations are still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leaf...
Preprint
Full-text available
Over recent years, major progress in experimental approaches have bring insights about the ecological functions of volatile organic compounds (VOCs) released by plants. However, deciphering volatile emissions in a methodologically robust and ecologically relevant manner remains a challenging issue. A surge in interest is required to characterize po...
Article
Phenolamides represent a family of specialized metabolites, consisting of the association of hydroxycinnamic acid derivatives with aliphatic or aromatic amines. Since the discovery of the first phenolamide in the late 1940’s, decades of phytochemical analyses have revealed a high structural diversity for this family and a wide distribution in the p...
Article
Full-text available
Phenolamides constitute a family of metabolites, widely represented in the plant kingdom, that can be found in all plant organs with a predominance in flowers and pollen grains. They represent a large and structurally diverse family, resulting from the association of phenolic acids with aliphatic or aromatic amines. Initially revealed as active com...
Article
1.Density‐ and trait‐mediated indirect interactions (DMIIs and TMIIs, respectively) are important drivers of community dynamics but how plant nutrients (bottom‐up effects) affect the magnitude of such indirect interactions has been scarcely addressed. 2.To assess the impact of bottom‐up forces on indirect interactions, we carried out lab and greenh...
Article
Full-text available
Soil abiotic factors and plant traits are able to trigger bottom-up effects along the tri-trophic plant–herbivore–natural enemy interactions. The consequences could be useful for controlling the insect herbivores. The South American tomato pinworm, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), a devastating invasive leaf-mining pest on tomato a...
Article
Full-text available
In the original publication of the article, the article type was incorrectly published as “Original Paper”. However, the correct article type is “Review Paper”.
Article
Full-text available
Stresses such as wounding or atmospheric pollutant exposure have a significant impact on plant fitness. Since it has been widely described that the metabolome directly reflects plant physiological status, a way to assess this impact is to perform a global metabolomic analysis. In this study, we investigated the effect of two abiotic stresses (mecha...
Article
Full-text available
Ficus species have adapted to diverse environments and pests by developing physical or chemical protection strategies. Physical defences are based on the accumulation of minerals such as calcium oxalate crystals, amorphous calcium carbonates and silica that lead to tougher plants. Additional cellular structures such as non-glandular trichomes or la...
Chapter
Root polyphenols have emerged through evolutionary processes to contribute to the specific roles assigned to the root compartment, i.e. plant nutrition, communication with symbionts, defence against soil‐borne aggressors and other allelopathic mechanisms like plant–plant interactions. Compared to aerial plant parts, roots often exhibit unusual poly...
Article
Full-text available
Foliar pathogens face heterogeneous environments depending on the maturity of leaves they interact with. In particular, nutrient availability as well as defense levels may vary significantly, with opposing effects on the success of infection. The present study tested which of these factors have a dominant effect on the pathogen's development. Popla...
Technical Report
Full-text available
Summarizing the SUNNIVA project
Presentation
Full-text available
Overview of project Sunniva - Sustainable food production through quality optimized rawmaterial production and processing technologies for premium quality vegetable products and generated by-products - SusFood Era-net FP7
Conference Paper
SUNNIVA addressed the entire food supply chain for tomatoes, Brassica and their derived products. The work packages focussed sustainability by using high-throughput non-destructive optical tools to optimize harvest time and determine the effects of elicitor treatments during pre-processing storage (WP1); effect of subsequent innovative processing o...
Article
Industrial processing of fruit and vegetables can have detrimental effects on health-promoting phytochemicals. Here, a novel pilot-scale process using an innovative spiral-filter press followed by a thermal treatment was evaluated for the production of tomato juice. Three-month storage of the resulting juice was also evaluated. The process impact o...
Article
The leafminer Tuta absoluta (Meyrick) is a major pest of the tomato crop and its development rate is known to decline when nitrogen availability for crop growth is limited. Because N limitation reduces plant primary metabolism but enhances secondary metabolism, one can infer that the slow larval development arises from lower leaf nutritive value an...
Article
This study aimed to assess the biological activities and secondary compound composition from Algerian Crithmum maritimum and therefore to better characterize local medicinal plants. The aerial parts were investigated regarding their mineral, antioxidant and essential oil content and composition. The C. maritimum aerial parts contained high levels o...
Article
Full-text available
Comparative phylogenetic analyses of the R2R3-MYB transcription factor family revealed that five subgroups were preferentially found in woody species and were totally absent from Brassicaceae and monocots (Soler et al., 2015). Here, we analyzed one of these subgroups (WPS-I) for which no gene had been yet characterized. Most Eucalyptus members of W...
Article
Wood, also called secondary xylem, is a specialized vascular tissue constituted by different cell types that undergo a differentiation process involving deposition of thick, lignified secondary cell walls. The mechanisms needed to control the extent of lignin deposition depending on the cell type and the differentiation stage are far from being ful...
Article
In plants, the partitioning of carbon resources between growth and defense is detrimental for their development. From a metabolic viewpoint, growth is mainly related to primary metabolism including protein, amino acid and lipid synthesis, whereas defense is based notably on the biosynthesis of a myriad of secondary metabolites. Environmental factor...
Article
Wild olive trees, also called oleasters, are largely distributed all around the Mediterranean basin. The objective of this study was to evaluate the quality, the composition and the antioxidant activity of four Algerian oleaster oils in comparison to an extra virgin olive oil (EVOO) reference. Three of the four oleaster oils exhibited a composition...
Article
Induced chemical defence is a cost-efficient protective strategy, whereby plants induce the biosynthesis of defence-related compounds only in the case of pest attack. Plant responses that are pathogen specific lower the cost of defence, compared to constitutive defence. As nitrogen availability (N) in the root zone is one of the levers mediating th...
Article
Plant-insect interactions are strongly modified by environmental factors. This study evaluates the influence of nitrogen fertilisation on the tomato plants (Solanum lycopersicum L.) cv. Santa clara and the leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Greenhouse grown tomato plants were fed hydroponically on a complete nutrient solu...
Article
Keywords: Juniperus oxycedrus Root bark Antioxidant activity Haemolysis activity HPLC–MS n a b s t r a c t This study investigates the phenolic compound content antioxidant and haemolytic activities in four extracts (methanol, water, hexane and dichloromethane) of Juniperus oxycedrus subsp. oxycedrus root bark. The methanol extract was the most con...
Book
he SUNNIVA project aims to increase the overall sustainability of vegetable processing by providing valorisation strategies to reduce waste and limiting environmental impact, while improving the nutritional properties of vegetable food products. Results obtained during the first project year indicate that; (i) The waste and by-product fractions of...
Article
Full-text available
Comparative genomics analysis unravels lineage-specific bursts of gene duplications related to the emergence of specialized pathways. The CYP76C subfamily of cytochrome P450 enzymes is specific to Brassicaceae. Two of its members were recently associated with monoterpenol metabolism. This prompted us to investigate the CYP76C subfamily genetic and...
Article
Phenolics are implicated in the defence strategies of many plant species rendering their concentration increase of putative practical interest in the field of crop protection. Little attention has been given to the nature, concentration and distribution of phenolics within vegetative organs of tomato (Solanum lycopersicum. L) as compared to fruits....
Article
The seed composition of four varieties of Opuntia ficus-indica growing in Algeria was investigated. Seeds ground into a fine powder were first, subjected to oil extraction and fatty acids analysis. The phenolic compounds were then extracted from the defatted powder of seeds in order to be quantified and characterised by liquid chromatography couple...
Article
Plant growth and defence are both fuelled by compounds synthesized from a common pool of carbon and nitrogen, implying the existence of a competition for carbon and nitrogen allocation to both metabolisms. The ratio of carbon to nitrogen (C:N) of an organ is often regarded as a convenient indicator of growth and quality. The purpose of this work wa...
Article
Coumarins are important compounds that contribute to the adaptation of plants to biotic or abiotic stresses. Among coumarins, umbelliferone occupies a pivotal position in the plant phenylpropanoid network. Previous studies indicated that umbelliferone is derived from the ortho-hydroxylation of p-coumaric acid by an unknown biochemical step to yield...
Article
High concentrations of phenolics have been shown to play a role in plant resistance to pathogens. One way to obtain increased phenolic concentrations in plant tissues is to limit mineral nitrogen (N) availability; however, over long periods, this treatment will have a negative effect on plant growth. The aim of our study was to determine the effect...
Article
Phenolic compounds are secondary metabolites involved in plant innate chemical defence against pests and diseases. Their concentration varies depending on plant tissue and also on genetic and environmental factors, e.g. availability of nutrient resources. This study examines specific effects of low (LN) and high (HN) nitrogen supply on organ (root,...
Article
Coumarins are important compounds that contribute to the adaptation of plants to biotic or abiotic stresses. Among coumarins, umbelliferone occupies a pivotal position in the plant phenylpropanoid network. Previous studies indicated that umbelliferone is derived from the ortho-hydroxylation of p-coumaric acid by an unknown biochemical step to yield...
Article
Full-text available
Understanding the regulation of the flavonoid pathway is important for maximising the nutritional value of crop plants and possibly enhancing their resistance towards pathogens. The flavonoid 3'5'-hydroxylase (F3'5'H) enzyme functions at an important branch point between flavonol and anthocyanin synthesis, as is evident from studies in petunia (Pet...
Data
Absorption maximum for substrates and products. HPLC absorption maximum for substrates and products used.
Data
Structures of substrates and products. Structures for substrates and products.
Article
Full-text available
The biosynthesis of linear and angular furanocoumarins is still poorly understood at the molecular level, with only psoralen synthase (CYP71AJ1) identified from Ammi majus. Using cDNA probes inferred from CYP71AJ1, three orthologs were isolated from Apium graveolens (CYP71AJ2) and Pastinaca sativa (CYP71AJ3 and -4) and functionally expressed in yea...
Article
Full-text available
Cynara cardunculus L. is an edible plant of pharmaceutical interest, in particular with respect to the polyphenolic content of its leaves. It includes three taxa: globe artichoke, cultivated cardoon, and wild cardoon. The dominating phenolics are the di-caffeoylquinic acids (such as cynarin), which are largely restricted to Cynara species, along wi...
Article
Full-text available
Ammi majus L. accumulates linear furanocoumarins by cytochrome P450 (CYP)-dependent conversion of 6-prenylumbelliferone via (+)-marmesin to psoralen. Relevant activities, i.e. psoralen synthase, are induced rapidly from negligible background levels upon elicitation of A. majus cultures with transient maxima at 9-10 h and were recovered in labile mi...
Article
Full-text available
Coumarins (1,2-benzopyrones) are ubiquitously found in higher plants where they originate from the phenylpropanoid pathway. They contribute essentially to the persistence of plants being involved in processes such as defense against phytopathogens, response to abiotic stresses, regulation of oxidative stress, and probably hormonal regulation. Despi...
Article
Full-text available
Coumarins (1,2-benzopyrones) are ubiquitously found in higher plants where they originate from the phenylpropanoid pathway. They contribute essentially to the persistence of plants being involved in processes such as defense against phytopathogens, response to abiotic stresses, regulation of oxidative stress, and probably hormonal regulation. Despi...
Article
Cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) complete cDNA was cloned from the leaves of Ruta graveolens, a psoralen producing plant. The recombinant enzyme (classified CYP73A32) was expressed in Saccharomyces cerevisiae. Mechanism-based inactivation was investigated using various psoralen derivatives. Only psoralen and 8-methoxypsoralen were found...

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Projects

Project (1)
Archived project
We aim at the development of a sustainable food system from production to consumption, addressing the entire food supply chain for the vegetables tomato and Brassica (white cabbage, cauliflower and broccoli), and their derived products. The project will increase consumers’ access to safe, healthy and convenient food through novel processing techniques, and improve utilization of raw material, by-products and waste, for which valorization strategies will be developed. Brassica and tomatoes represent a major part of the human diet. They are consumed world-wide and are renowned for their health beneficial effects. In Europe, tomatoes and Brassica are among the most important vegetables cultivated; the areas for Brassica and tomato production cover 400 000 and 250 000 hectares, respectively, yielding 6 and 15 million tons/year. Preservation of health-beneficial phytochemicals (HBPC) is central in the vegetable processing part of the project. It is technologically challenging to preserve the nutritional and sensory quality of vegetables in processed foods, e. g. provide for acceptable texture, color and health-promoting compounds. Process optimization by modeling, via knowing the temperature distribution of the products during processing, will contribute to preserve HBPC and thus food quality. Tomato and Brassica have a high intrinsic health-promoting value and technologies will be developed to preserve the high HBPC level in the derived food products while also developing novel products with beneficial nutritional and sensory attributes. The initial properties of the vegetable raw material before processing is a limiting factor for the quality of the food products derived thereof. Optimal harvest time and post-harvest elicitor treatments will further increase HBPC in the raw material. We will also explore the use of well-balanced organic N-fertilizer, based on unused vegetable biomass, and enhanced with bio-control microorganisms. Promoting plant health and growth will result in benefits for the consumer, and for the plant (higher phytochemical content is associated with higher disease resistance). This convergence of benefits includes lower cost and reduced environmental pollution. Non-destructive, high throughput optical indices will be used to monitor phytochemicals, reducing the use of toxic chemicals for wet chemistry analysis. The global volume of vegetable food wastage, not including agricultural waste, is estimated to 400 million tons/year. Valorization of unused biomass after processing thus enhances food production sustainability and contributes to a lower ecological impact. We will reduce waste in the food supply chain using two strategies. First, processing and stabilization for recycling into the food chain will be investigated through the use of the novel technologies spiral press filtering and refractance window drying. Second, the value of unused vegetable biomass as component in the production of organic fertilizer will be evaluated. The development of innovative processing technologies is essential for improving competitiveness and economic growth for the European food industry. The novel food processing technologies microwave heating and agitated retorting will be utilized to demonstrate the potential for combining savings in energy and water consumption.