Rolish Singh

• Doctor of Philosophy
• Scientist at Fera Science Ltd. York

Four criconematid populations were uncovered in three avocado orchards in Homestead region, Miami-Dade County, Florida, USA, with three of them in relatively high densities. Two of the populations were identified as Criconema mutabile (Taylor, 1936) Raski & Luc, 1985 and Mesocriconema basili (Jairajpuri, 1964) Loof & De Grisse, 1989, whereas the ot...

A survey for slug- and snail-associated nematodes was conducted in forests, parks, botanical gardens, and nature reserves at 13 localities in Belgium to uncover more diversity of gastropod mollusc-associated nematodes and to characterise Pellioditis populations found in the country. A total of 319 slugs and snails belonging to nine species were exa...

Paratylenchus cacao n. sp., a pin nematode associated with cocoa (Theobroma cacao L.) from Ghana, is described herein based on morphology, morphometrics and phylogenetic relationships with other species. Morphologically, P. cacao n. sp. is characterised by a female body length of 174-265 μm, body assuming a ‘C’ or ‘J’ shape after heat relaxation, a...

The stunt nematode species, Tylenchorhynchus annulatus (Cassidy, 1930) Golden, 1971, is a widely distributed plant parasite found associated with various major agricultural crops. In the current work, a population of T. annulatus detected from a sugarcane field in Everglades, Florida, USA, was morphologically and molecularly characterised. A compar...

A new root-knot nematode (RKN) species, Meloidogyne karsseni n. sp., associated with sweet pepper from Mexico, and a population of M. paranaensis from Guatemala, are described using data from morphological, biochemical (isozyme enzymes), molecular, and phylogenetic analyses. Meloidogyne karsseni n. sp. can be morphologically diagnosed using the com...

Guava is a nutritious and profitable high-yield crop. Guava root-knot nematode (GRKN), M. enterolobii Yang & Eisenback, 1983 (= M. mayaguensis Rammah & Hirschmann, 1988), cause detrimental damage in guava orchards in more than 15 countries. Fifteen guava orchards in Homestead, Florida, were randomly sampled to check for potential plant-parasitic ne...

Rotylenchus bunae n. sp. was discovered in the coffee rhizosphere in Gera district of Jimma, Ethiopia, and has been morphologically and molecularly characterised. The new species is identified by a female body length of 725-876 μm, hemispherical lip region with 4-5 lip annuli, stylet length of 28-30 μm, lateral field areolation only in the pharynge...

The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus , are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC a...

Analysis of a soil sample from Aizawl district of Mizoram, India, led to the discovery of Mulveyellus aizawlensis sp. n. The new species is presented with female morphological data and molecular data of the D2-D3 expansion segment of 28S and 18S of rDNA, and phylogenetic analysis data based on these sequences. Mulveyellus aizawlensis sp. n. is morp...

Nematological investigation of the order Tylenchida, super-family Criconematoidea and sub-family Macroposthoniinae revealed the presence of two species of the genus Mesocriconema i.e., Mesocriconema mamitensis n. sp. and M. medani, in the rhizospheres of lemongrass and pineapple, respectively, from Mamit district of Mizoram, India. Mesocriconema ma...

Passion fruit (Passiflora edulis) is one of the tropical fruits well-adapted to Florida’s hot and humid climate. A high population of root-knot nematode, Meloidogyne incognita was identified morphologically from soil samples taken from declined passion fruit vines in an organic field in North Florida. Species identification was also confirmed based...

A study of coffee rhizospheres collected from six coffee fields in the Gera and Gomma districts of Jimma Zone, southwest Ethiopia, revealed the presence of 12 genera of plant-parasitic nematodes, including Belonolaimus, Criconemoides, Cryphodera, Discocriconemella, Helicotylenchus, Longidorus, Ogma, Paratylenchus, Rotylenchus, Scutellonema, Tylench...

A study of coffee rhizospheres collected from six coffee fields in the Gera and Gomma districts of Jimma Zone, southwest Ethiopia, revealed the presence of 12 genera of plant-Xiphinema were the most prevalent, detected in all soil samples with up to 1590 individuals 100 ml −1 soil for Cryphodera. Morphological and molecular analysis of three genera...

Paratylenchus ilicis n. sp. was found associated with holly in the Netherlands and was described based on morphology, morphometrics, rRNA and mitochondrial cox1 genes, phylogenetic relationships with other Paratylenchus species, host information and geographical distribution. This species can be morphologically diagnosed based on its light brown, s...

A population consisting of only second-stage juveniles of Cryphodera kalesari (Nematoda: Heteroderidae) was detected in the rhizosphere of bamboo in a forest in Mizoram state of India. New taxonomical information is provided for the juveniles, including the first light microscopy and scanning electron microscopy images. The juveniles are characteri...

The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora, is an important biological control agent worldwide. Industrially produced EPN need to meet the climatic requirements for the control of pests in field agriculture in autumn and spring when temperatures are low. For this trait (virulence at low temperature), previous EPN improvement...

Plant-parasitic nematodes (PPN) surveys have been conducted in both agricultural and natural habitats, including samples from sugarcane (Kilimanjaro region of Tanzania), coffee (Jimma region of Ethiopia), finger millet (Eldoret region of Kenya), corn (eastern province of Rwanda), sand dunes (coastal beach of Gran Canaria) and other selected places...

Gastropods are extremely diverse taxa comprising nearly 60,000 species, of which, the giant African land snail, Achatina fulica is a well-known member. It is an invasive species, and it serves as vector and host for several free-living and parasitic nematodes. This makes studies on the diversity of nematodes isolated from gastropods be more emphasi...

This datasheet on Tylenchorhynchus crassicaudatus covers Identity, Overview, Distribution, Dispersal, Hosts/Species Affected, Diagnosis, Biology & Ecology, Impacts, Prevention/Control, Further Information.

Rotylenchus is a widely-distributed economically important plant-parasitic nematode group whose species-level identification relies largely on limited morphological characters including character-based tabular keys and molecular data of ribosomal and mitochondrial genes. In this study, a combined morphological and molecular analysis of three popula...

Rotylenchus wimbii n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and COI of mitochondrial DNA. This new species is characterized by a moderate female body size...

Pin nematodes of the genus Paratylenchus are obligate ectoparasites of a wide variety of plants that are distributed worldwide. In this study, individual morphologically vouchered nematode specimens of fourteen Paratylenchus species, including P. aculentus, P. elachistus, P. goodeyi, P. holdemani, P. idalimus, P. microdorus, P. nanus, P. neoamblyce...

Heterodera dunensis n. sp. from the coastal dunes of Gran Canaria, Canary Islands, is described. This new species belongs to the Schachtii group of Heterodera with ambifenestrate fenestration, presence of prominent bullae, and a strong underbridge of cysts. It is characterized by vermiform second-stage juveniles having a slightly offset, dome-shape...

Using morphological, morphometric and molecular analysis, eleven valid nematode species from nine genera: Amplimerlinius icarus, Criconema annuliferum, Criconemoides informis, Helicotylenchus varicaudatus, Hemicriconemoides pseudobrachyurus, Hemicycliophora thienemanni, Mesocriconema xenoplax, Paratylenchus bukowinensis, P. nanus, Rotylenchus monta...

Morphological and molecular analyses of plant - parasitic nematodes (PPN) from 12 sugarcane plantation sites of Tanganyika Planting Company (TPC) Limited in Kilimanjaro region of Tanzania revealed the presence of six PPN genera, i.e. Helicotylenchus , Hemicycliophora , Pratylenchus , Rotylenchulus , Scutellonema , and Tylenchorhynchus . The genera...

A study of plant-parasitic nematodes in the Botanical garden at Ghent University in Belgium revealed the presence of two tropical nematode species, i.e. Scutellonema brachyurus and Meloidogyne incognita. Scutellonema brachyurus was recovered only once for the first time in Belgium from Musa basjoo and is morphologically characterized. M. incognita,...

During a survey in the Botanical garden of Ghent University, a new species Rotylenchus rhomboides n. sp. and a population of Rotylenchus buxophilus were found. Rotylenchus rhomboides n. sp. is characterized by the presence of a rhomboid-like widening of the midridge of lateral field at the level of vulva, a feature previously unknown within the ge...

Root-lesion nematodes, Pratylenchus spp., are one of the most important nematode groups in economic terms. The combination of morphological analyses and molecular analyses based on D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA regions supported the establishment of a new Pratylenchus species, making a total of 103 valid Pratylenchus species. The femal...

A survey for slug-associated nematodes in five locations of East and West Flanders in Belgium revealed the presence of one new and six known slug-parasitic nematodes, Agfa flexilis (Dujardin, 1845), Alloionema appendiculatum (Schneider, 1859), Angiostoma dentiferum (Mengert, 1953), Angiostoma limacis (Dujardin, 1845), Angiostoma norvegicum (Ross et...

Pratylenchus rwandae n. sp., a root-lesion nematode associated with maize (Zea mays) from Rwanda, is described. This new species is characterised by females of medium to large size (469-600 μm) having an offset lip region with three annuli, stylet of 13-14.6 μm long with prominent rounded or anteriorly concave knobs, short to long pharyngeal gland...