Roland Sanchez

Roland Sanchez
Viñedos Emiliana · enologia y sustentabilidad

PhD

About

30
Publications
8,196
Reads
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341
Citations
Citations since 2016
24 Research Items
259 Citations
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050

Publications

Publications (30)
Article
Larval dispersal affects population dynamics and thus the evolution of species; therefore, understanding how far planktonic larvae can move is key for the conservation and management of marine species. The degree to which genetic connectivity is temporally stable in marine environments is still largely unknown. Here we evaluated the temporal stabil...
Article
Full-text available
Phyllosphere bacteria have received little attention despite their important roles in shaping plant performance traits. In this study, we characterize the bacterial communities on leaves of native trees inhabiting sclerophyllous forests in central Chile, one of the world's biodiversity hotspots. Additionally, we provide profiles of bacterial commun...
Article
Full-text available
Agriculture is one of the main drivers of land conversion, and agriculture practices can impact on microbial diversity. Here we characterized the phyllosphere fungal diversity associated with Carménère grapevines under conventional and organic agricultural management. We also explored the fungal diversity present in the adjacent sclerophyllous fore...
Data
Rarefaction plots for ITS2 and D2 fungal amplicons Rarefaction plots indicating the number of OTUs for (A) ITS2 and (B) D2. Panel C shows the relationship between phylogenetic diversity and the number of D2 reads. Samples were collected from forest leaves (dark green), vine leaves (green), and grape berries (Purple).
Data
Comparison of relative abundances of species between vine and native forest leaves using ANOVA This table indicates the taxonomic assignment of each OTU, the F statistic and its P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR), and the mean relative abundance of each species in the vineyards and forest. Green row...
Data
Comparison of relative abundances of operational taxonomic units (OTUs) between vine leaves and native forest leaves This table indicates the OTU identity, the normalized mean between groups, the log2 fold change and its standard error, the Wald statistics and their P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR...
Data
Comparison of relative abundances of operational taxonomic units (OTUs) between conventional and organic vineyards This table indicates the OTU identity, the normalized mean between groups, the log2 fold change and its standard error, the Wald statistics and their P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR),...
Data
Comparison of relative abundances of species between grape berries and vine leaves using ANOVA This table indicates the taxonomic assignment of each OTU, the F statistic, and its P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR), and the mean relative abundance of each genus in vineyards and forest. Green rows ind...
Data
Comparison of relative abundances of species between conventional and organic vineyards using ANOVA This table indicates the taxonomic assignment for each OTU, the F statistic and its P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR), and the mean relative abundance of each species in the vineyards and forest. No...
Data
Comparison of relative abundances of operational taxonomic units (OTUs) between grape berries and vine leaves This table indicates the OTU identity, the normalized mean between groups, the log2 fold change and its standard error, the Wald statistics and their P-values, the P-value corrected for multiple comparisons (False Discovery Rare; FDR), and...
Preprint
Full-text available
Agriculture is one of the main drivers of land conversion and agriculture practices can impact on microbial diversity. Here we characterized the phyllosphere fungal diversity associated with Carmenere grapevines under conventional and organic agricultural management. We also explored the fungal diversity present in the adjacent sclerophyllous fores...
Preprint
Full-text available
Agriculture is one of the main drivers of land conversion and agriculture practices can impact on microbial diversity. Here we characterized the phyllosphere fungal diversity associated with Carmenere grapevines under conventional and organic agricultural management. We also explored the fungal diversity present in the adjacent sclerophyllous fores...
Article
Full-text available
The edible sea urchin Loxechinus albus (Molina, 1782) is a keystone species in the littoral benthic systems of the Pacific coast of South America. The international demand for high-quality gonads of this echinoderm has led to an extensive exploitation and decline of its natural populations. Consequently, a more thorough understanding of L. albus go...
Article
Full-text available
The northern part of the Chiloé Island (41.8°S) has been linked to an abrupt biogeographic break in the Southeastern Pacific; a break which represents the northern limit of the Magellanic Province, a large zone geographically characterized by a broken coastline as opposed to the Chilean coast further north. This study represents a first eco-biogeog...
Article
Full-text available
The Loxechinus albus is the only sea urchin exploited for commercial fisheries and is an important component of communities across southeastern Pacific coast. Despite its ecological and economic importance, limited information is known about genetic diversity and the actual connectivity among their populations. From 43,608 contigs obtained from the...
Article
Full-text available
Deciphering ecological effects of major catastrophic events such as earthquakes, tsunamis, volcanic eruptions, storms and fires, requires rapid interdisciplinary efforts often hampered by a lack of pre-event data. Using results of intertidal surveys conducted shortly before and immediately after Chile's 2010 M(w) 8.8 earthquake along the entire rup...
Data
Geographic coordinates of the rocky shore sites visited to estimate land-level changes. (DOC)
Data
Geographic coordinates of the sandy beaches studied indicating the types of sites sampled at each beach. (DOC)
Article
The marine gastropod Concholepas concholepas, locally known as the "loco", is the main target species of the benthonic Chilean fisheries. Genetic and genomic tools are necessary to study the genome of this species in order to understand the molecular basis of its development, growth, and other key traits to improve the management strategies and to...
Data
Three biogeographic areas have been recognized along the Chilean coast, with biogeographic breaks located at 30 and 42 degrees S allowing us to test the concordance between biogeographic patterns and spatial patterns of genetic and morphological diversity in marine species. We examined the marine gastropod Acanthina monodon, whose range spans the 2...

Questions

Questions (4)
Question
Hi,
I'll do my first run in Miseq Illumina. I am using this standard protocol: "Metagenomic Sequencing 16S Library Preparation ". I'll analyze 50 samples, each sample is three amplicons mixed (each approximately 350 bp), I will use a Nextera XT and  2x250 cycles kit, but in previous runs the total second read (reverse) had low quality, why does that happen? How can I avoid this in my run?
Thanks.
Question
I want make a amplicon sequencing for the first time in a Illumina MiSeq using Nextera XT kit. I have three amplicons of 300 bp average (1 prokaryotic and 2 eukaryotic). original Nextera XT protocol suggests only add the 1) primers sequence and 2) overhang adapter sequence for any amplicon. But some articles using Nextera and add 1) primers sequence, 2) overhang adapter sequence, 3) a two basepair ‘‘linker’’ sequence designed to mismatch against all major lineages immediately upstream of the gene primer and 4) “barcode sequence”, Should I do this also in Nextera XT???. Are dispensable the steps 3 and 4 ?
Question
I want make a amplicon sequencing for the first time in a Illumina MiSeq. I want to get equal coverage per sample. Do I need to standardize the initial amount of DNA in the PCR? Or I just have to standardize the amount of post-pcr product?. Thanks.

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