About
228
Publications
76,576
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
4,075
Citations
Introduction
Microbiologia
Produtos Naturais
Enzimas
Propriedades Biológicas
Publications
Publications (228)
Sausage is an emulsified meat product that, due to its composition, undergoes physicochemical changes during its shelf life, which makes the use of additives for its conservation necessary. The goal of the present study was to evaluate the antioxidant capacity of rosemary extract, acerola extract, and a mixture of tocopherols applied to industriali...
The objective of this study was to evaluate the chemical composition and antifungal activity of free and encapsulated Cinnamomum cassia essential oil (EO) against Penicillium crustosum, Alternaria alternata, and Aspergillus flavus, and the aroma persistence in maize flour. Trans-cinnamaldehyde (TC) was identified as the major compound (86 %) in the...
Hop essential oil and hop extract using carbon dioxide (CO2) are products with high added value because they have bioactive and sensory properties. In this context, the objective of this study was to obtain and characterize essential oil and extracts from pelleted hops of El Dorado, Polaris, Hallertau Blanc and Callista varieties using hydrodistill...
Microencapsulation is an alternative to increase the survival capacity of microorganisms, including Yarrowia lipolytica, a widely studied yeast that produces high-value metabolites, such as lipids, aromatic compounds, biomass, lipases, and organic acids. Thus, the present study sought to investigate the effectiveness of different wall materials and...
Background: Yerba-mate (Ilex paraguariensis) is a perennial native tree from South America. Improper management of yerba-mate crops results in low yield. This work evaluated the recovery and the vegetative capacity of the crop after coppicing. Methods: A 2-year field observation approach was used and the study area was monitored from 2019 to 2021 i...
This study evaluated the action of commercial and non-commercial cellulases and pectinases in the hydrolysis of soybean hulls (SH) and corn stover and cobs (CSC), the effect of temperature and agitation on the lignocellulosic substrate hydrolysis and the bromatological characteristics of hydrolyzed substrates. The effect of pretreatment on the hydr...
This work aimed to estimate the mass transfer parameters of guabiju pulp under different temperatures of drying, modeling the kinetic curves using Fick's second law and the Lewis, Henderson–Pabis, Page, Modified Page, Logarithmic and Midilli models and to evaluate their biological properties and color. Guabiju pulp were dried at 313.15–373.15 K. Th...
The waste consisting of banana peel represents a significant fraction of the processing industry of this fruit, characterizing an environmental problem. On the other hand, banana peels contain several minerals and nutrients that are beneficial to health. In this sense, the objective of this work was to study the thin layer drying of green banana pe...
The use of pesticides has grown over the years and their effects on ecosystems, especially aquatic ones, have been increasingly observed. Agricultural pesticides are responsible for a large part of pollution in aquatic environments, and can reach non-target organisms, harming biodiversity. In this research, the effects of common pesticides on morta...
Key message
The mechanisms of morpho-physiological adaptation to different conditions of light and flooding are species-specific; however, light is more responsive than flooding for two species of the genus Eugenia.
Abstract
The frequency of flooding in subtropical riverine forests is responsible for the occurrence of the tree species, being assoc...
O objetivo do trabalho foi avaliar a atividade antioxidante, o teor de compostos fenólicos e flavonoides e a atividade antibacteriana de diferentes extratos de semente de Eugenia involucrata obtidos por extrações sucessivas em Soxhlet e por maceração, utilizando os solventes hexano e álcool etílico. A atividade antioxidante foi fundamentada na medi...
Pereskia aculeata Mill., popularmente conhecida como ora-pro-nobis e “carne-de-pobre”, devido ao teor de proteína das folhas, é considerada planta alimentícia não-convencional (PANC). O trabalho teve como objetivo a caracterização físico-química das folhas de P. aculeata e a avaliação da atividade antioxidante e conteúdo de compostos fenólicos em s...
The objective of this work was to develop, characterize and evaluate the application of active edible films based on gelatin and green tea extract in coating of fresh sausages. The green tea extract showed IC50 of 0.088 mg/mL and minimum inhibitory concentrations of 0.05 mg/mL for Listeria monocytogenes, 0.025 mg/mL for Staphylococcus aureus, 0.04...
Compounds that reduce or neutralize free radicals have been evaluated for use as nutraceutical or antioxidant additives in processed foods. This study aimed to enzymatically produce ascorbyl oleate and assess its biological properties. The synthesis was performed under previously maximized conditions (L-ascorbic acid/oleic acid 1:9 molar ratio, 70...
The increasing incidence of foodborne illnesses worldwide has become an economic and public health problem. In order to reduce the risks associated with food safety, the Normative Instruction n° 79, 2018, modified the inspection of jowl in pork slaughterhouses in Brazil and requires the submission to validated treatments such as heat or other metho...
This work aimed to produce collagens and hydrolysates with antimicrobial and antioxidant activity from sheep slaughter by-products. The by-products (sheep and lamb) were treated and extracted. The collagens were hydrolyzed with the enzyme Alcalase®. The spectra of collagens and hydrolysates were similar (amide bands I, II, III, A, B). The bands pre...
Bioactive compounds from plant extracts can be sensitive to extraction conditions, especially at high temperatures, so extraction techniques that use milder processing conditions are intensively studied and applied. Among these techniques, extraction with pressurized liquids (PLE) stands out as an efficient method widely used to obtain biocompounds...
Em fragmentos florestais subtropicais, a espécie Hovenis dulcis apresenta alto grau de invasibilidade. A alelopatia pode causar efeitos negativos sobre a germinação e desenvolvimento de espécies nativas, como a redução da germinação e no do crescimento radicular. O objetivo deste trabalho foi avaliar o efeito fitotóxico de extratos foliares e de ps...
Introdução:
as indústrias de processamento de frutas geram grande quantidade de biomassa residual que poderia ser reaproveitada. Considerando o elevado volume de resíduos produzidos pelo descarte das sementes do abacate e destacando o alto teor de compostos bioativos, é um produto interessante para ser avaliado.
Objetivo:
avaliar a composição cent...
Este estudo investigou a recuperação de atividades fitoquímicas e biológicas em extratos de sementes de Plantago major e Plantago tomentosa utilizando diferentes solventes. Por meio da extração sequencial e fracionada a frio, utilizando solventes com polaridade crescente (n-hexano, diclorometano e etanol) na recuperação de polifenóis totais, flavon...
Kaolin is a clay mineral with several applications, especially as filler in the paper industry. Brazil has the largest kaolin processing plant in the world located in the Amazon region. Kaolin mining activities generate high volumes of waste, resulting in additional costs and environmental impacts, and therefore, new approaches for their reduction...
Blueberry by-products are rich in bioactive compounds with antioxidant properties, such as phenolic compounds and anthocyanins. Pressurized liquid extraction (PLE) is an alternative to traditional methods in the obtention of bioactive compounds and can adjust pressure and temperature conditions to regulate the solubility of target compounds in the...
Polyethylene glycol (PEG) is an ingredient for approved drug products by the Food & Drug Administration. PEG is mostly used as a pharmaceutical adjuvant, ophthalmic and oral acts as an encapsulating agent, plasticizing agent in food packages, modified and/or functionalized materials, among other applications. It is important to know the molecular m...
A Kombucha é uma bebida obtida pela fermentação de chá verde e/ou preto adicionada de uma fonte de açúcar, submetida a uma fermentação simbiótica entre leveduras e bactérias presentes nos botânicos agregados ou de cultura previamente selecionada e ou armazenada. A crescente popularidade da kombucha deve-se as propriedades antioxidantes, vitaminas d...
This study proposes a review of biological potential of cinnamon (Cinnamomum cassia) essential oil with a focus on microencapsulation as an alternative to control the occurrence of pests in stored maize grains. Due to the demands on corn productivity, there is the need to improve grain storage processes and conditions, since that in this stage ther...
The aim of this work was to evaluate the effects of the addition of swine skin on the technological characteristics of mortadella formulations produced on industrial scale. The effects of concentrations of swine skin (1.5 to 5.5 %) and sodium chloride (2 to 3 %) on total protein, total fat, starch, moisture, water activity, sodium, pH and texture p...
A restrição do uso de antibióticos como melhoradores de desempenho na avicultura de corte, levou as indústrias de nutrição animal a direcionar suas pesquisas por substâncias que possam ser alternativas a esses aditivos. A própolis, substância resinosa e balsâmica coletada pelas abelhas a partir de plantas, tem sido foco de estudos como aditivo natu...
Abstract Given possible temperature variations in the cold chain during retail display of chilled food, this work evaluated the growth of Salmonella choleraesuis and Staphylococcus aureus inoculated in chicken breast fillet submitted to different temperature abuses. The bacterial growth was evaluated in Luria–Bertani broth and previously inoculated...
The objective of this work was to evaluate the influence of temperature on the content of bioactive compounds of fruits of guabiju (Myrcianthes pungens (O. Berg) D. Legrand). The peel, pulp and seed of fresh guabiju were analyzed in relation to physical-chemical composition, metals, color, phenolic compounds, flavonoids, anthocyanins, vitamin C and...
The essential oil of citronella (Cymbopogon winterianus) has several biological activities, among them the insect repellent action. Some studies showed that cinnamic acid esters can be applied as natural pesticides, insecticides and fungicides. In this context, the objective of the present work was to evaluate the production of esters from citronel...
The packaging protects food from actions of external agents, from alterations and contaminations, in addition to adulteration. Edible films are structures produced from biopolymers, which can replace non-biodegradable packaging. These films have been formulated with naturally polymers of polysaccharides, lipids and proteins, isolated or combined wi...
O armazenamento de grãos de milho apresenta suscetibilidade à contaminação por fungos, que além de ocasionarem redução no rendimento, qualidade e perdas econômicas, podem produzir micotoxinas, que apresentam risco a saúde. O uso dos óleos essenciais (OE) apresenta-se como uma alternativa em potencial para a inibição do crescimento de fungos. Diante...
A cúrcuma (Curcuma longa) é usada como aditivo e corante na indústria de alimentos, por possuir excelentes propriedades biológicas. O objetivo do trabalho foi comparar as atividades antioxidante, antimicrobiana e teor de compostos fenólicos totais dos extratos obtidos de cúrcuma em pó (comercializada) e do rizoma da cúrcuma in natura. A extração fo...
Metals and agrochemicals are among the main aquatic contaminants, being able to trigger oxidative stress in exposed organisms. The objective of this work was to evaluate the correlation between the level of oxidative stress biomarkers in Aegla crabs (Crustacea, Anomura) with (i) the set of metals present in the streams sediment and (ii) with land u...
Sheep´s milk whey is an important source of protein and present functional properties. Studies related to the concentration of sheep´s milk whey proteins are still scarce in the literature. In order to improve not only the chemical, physical and functional properties of foods, but also the absorption characteristics of the proteins, without impairi...
In this work was optimized the production of benzyl cinnamate by enzymatic catalysis using the immobilized lipase NS88011 and to evaluate its biological properties. The optimized condition for this system was 1:3 (acid:alcohol) molar ratio, 59 °C, biocatalyst concentration 4.4 mg.mL⁻¹ for 32 h, with a yield of 97.6 %. The enzyme stability study sho...
Purpose
The objective of this study was to elaborate and characterize formulations of type mortadella bologna in an industrial unit.
Design/methodology/approach
Two formulations (Formulation 1 – F1 and Formulation 2 – F2) were characterized at a storage stability of 22 ºC for physicochemical (pH, a w , moisture, protein, fat and lipid oxidation: T...
This study aims to evaluate the influence of combined use of native cassava starch (CS) and modified cassava starches (MCS) on the quality of cheese bread formulations processed and frozen in an industrial system. The techno‐functional properties are evaluated on the dough and pieces of bread after baking. The formulation with the highest amylose c...
A new enzymatic-colorimetric time-temperature integrator (ECTTI) to evaluate temperature abuse in refrigerated products was developed with Tributyrin, pH indicator, and lipase Calb. The kinetic behavior of the ECTTI (0.01 µL.L⁻¹ enzyme) was assessed at 5, 10, 15, 20, and 25ºC, evaluating changes in pH (≤7.2) and color (ΔE ≥12). Storage at 5°C provi...
Food packaging materials compounded with antimicrobial additives can substantially diminish the incidence of foodborne diseases. Here, poly(L-lactic acid) (PLA) films containing sodium metabisulfite (NaM) were produced by melt extrusion as an attempt to develop a new biodegradable material with antimicrobial properties for packaging. Life cycle ass...
The present study seeks to evaluate different matrixes (chitosan, gelatin – bloom 189, gelatin – bloom 246, gum Arabic, and maltodextrin) to encapsulate Lactobacillus helveticus (LH 091), as well as optimal spray-drying conditions. L. helveticus displayed a survival rate close to 89%, with their viability above the minimum level of 10⁷ CFU/g at poi...
In this study, bisubstrate kinetics mechanism models were correlated with the experimental data of ascorbyl oleate using the new lipase preparation, Candida antarctica NS 88011, and kinetic parameters (Vmax, Km, and Ki) were estimated. The highest conversion (49.42 %) was observed at 1 h of reaction using the ascorbic acid/oleic acid molar ratio of...
Food with functional properties have sparked consumer interest due to health benefits. In this work, mint and blueberry were chosen to be added to chocolate as a source of bioactive compounds because they have functional properties. The aim of this study was to evaluate the physical-chemical characteristics (color, moisture content, water activity,...
The residue from chicken mechanically separated meat (MSM) is a potential source for the extraction of collagen. However, this process requires the removal of many covalent crosslinks, which makes it quite complex. Ultrasound has been successfully used to extract collagen; it reduces the process time and increases the yield. However, information re...
The chemical composition and biological properties of citronella essential oil were modified by the enzymatic esterification reaction of the major monoterpenic alcohols with cinnamic acid. The almost complete conversion of geraniol and citronellol present in the citronella (Cymbopogon winterianus) essential oil, into geranyl (99%) and citronellyl (...
This review compiles and discusses the use of genetic markers applied in the study of the fish genus Hypostomus Lacépède, 1803 (Siluriformes: Loricariidae). The database comprises 51 peer-review articles that were published in the last 52 years (1968–2020) and that approach analysis based on different classes of genetic markers. The use of cytogene...
(1) Background:
The aim of this study was to evaluate the production and partial characterization of xylanase and avicelase by a newly isolated Penicillium sp. in solid-state fermentation, using soybean hulls as substrate.
(2) Methods:
Temperature, time, number of spores, and substrate moisture on xylanase and avicelase bioproduction were evaluate...
A planta medicinal Ruta graveolens L. (RG), popularmente conhecida como arruda, já foi sugerida na literatura com potencial para o desenvolvimento de medicamentos para o tratamento de demência e da doença de Alzheimer. O objetivo deste trabalho foi investigar os efeitos da administração aguda do óleo essencial de RG (100 mg/kg) sobre a memória de c...
The aim of this work was to evaluate different concentrations of ethanol on antioxidant (phenolic and flavonoid) compounds extraction of Mentha x Piperita aiming at applying the extract in whey-based dairy beverages. The extracts were obtained by cold maceration using water and ethanol as solvents. The extract using water and 70% ethanol had a 13.8...
The aim of this study was to evaluate the synthesis of pectinase from Penicillium brasilianum in shake flasks and address their potential for industrial applications. A Plackett-Burman design followed by a complete second order design were used for the screening of most important factors and to maximize the polygalacturonase activity, respectively....
Glutathione Reductase (GR) plays a key role in the maintenance of GSH homeostasis, variations in its activity may indicate damage from environmental contaminants. Experimental planning is a strategy to reduces time and cost in enzymatic assays. In this work, DCCR experimental planning was used to standardize and to validate the measure of GR in Aeg...
The present study aimed to maximize the conventional extraction and compare it with the ultrasound-assisted method for extracting bioactive compounds obtained from the red araçá peel. The behavior of anthocyanins related to the pre-treatment of the vegetal matrix, employed solvent, extraction kinetics of both methods, the levels of total phenolic c...
Obtaining collagen and hydrolysates from mechanically separated chicken meat residue is an excellent way to add value to this waste. The aims of this study were to obtain collagen hydrolysates from chicken MSM residue using Alcalase® and Flavourzyme® enzymes, as well characterizing the functional, structural and thermal stability properties. The hi...
The perception of a basic education of the importance of agroecological agricultural systems for human and environmental health is fundamental for changes in consumption habits, the conservation of local biodiversity and long-term social transformation. We analyzed, by utilizing a questionnaire consisting of open and closed questions, the perceptio...
The principle and the techniques applied in DNA extraction play a pivotal role in the obtention of a purified genetic material. The present study investigates the efficiency of eight protocols in the DNA extraction of Hypostomus commersoni, an essential component of South American freshwater ichthyofauna. The quality of samples was assessed through...
Este trabalho tem como objetivo avaliar as características sensoriais, utilizando a análise descritiva quantitativa (QDA), de diferentes formulações de azeitonas verdes fermentadas por 120 dias com Lactobacillus plantarum ATCC 8014 ou culturas espontâneas (microbiota indígena). A fermentação foi realizada com solução salina em diferentes concentraç...
Nos últimos anos, a crescente demanda por produtos naturais na indústria de alimentos incentivou esforços notáveis no desenvolvimento de processos biotecnológicos para a produção de compostos aromatizantes. O presente estudo relata a maximização da produção de acetato de isoamila por esterificação de álcool isoamílico e ácido acético em um sistema...
There is a growing search for alternative raw materials to obtain collagen and hydrolysates and processes that do not threaten the environment or human health. Thus, sheep slaughter residue, which doesn't yet have an adequate and sustainable destination, is an excellent source of study. The objective of this study was to investigate the technologic...
The processing steps as scalding and de-feathering in the poultry industry have a great impact in meat quality. In this sense, the aim of this work is to evaluate the temperature effect (52, 57 and 62ºC), broiler weight (2.4-2.6, 2.8-3.0 and 3.2- 3.4 kg) and broiler processing line speed (4.000, 6.500 and 9000 broilers/h) of the scalding and de-fea...
The ultrasound effect on functional and structural properties of hydrolysates of different bovine collagens (fibers, gelatines and hydrolysates) was evaluated. The enzymatically hydrolyzed samples (enzyme Alcalase®) with previous or simultaneous ultrasound treatment presented higher number of bands with shorter wavelengths (mid-FTIR). The ultrasoun...
The objective of this work is to characterize two types of bovine collagen (fibre and powder), evaluating its application in mixed hamburger formulations, as well as the quality characteristics of the products. The collagen fibre had a fibrillar structure, molecular mass 100 kDa and greater gel strength (146 315 Pa) and protein content (97.81 %) th...
The principle and techniques applied in DNA extraction play a pivotal role in the obtention of a considerable and purified yield of genetic material. In this work, we evaluate the efficiency of seven protocols chosen from literature for the DNA extraction of Hypostomus commersoni (Valenciennes, 1836), an important component of South American freshw...
The clove essential oil is a natural antioxidant and antimicrobial with potential applications in many fields of the industry. Nanoemulsions (NEs) with Dp ~135 nm loaded with clove oil (CO) and crodamol were produced by ultrasound. The presence of crodamol in formulation increased droplet size homogeneity and prevented the diffusional degradation w...
This study evaluates the in vitro effect of commercial β-glucanase and chitinase in controlling the growth of undesirable fungal contaminants on salami surface, which can cause both changes in sensory aspects of the sausage and production of mycotoxins and antibiotics. Hence, we isolated and identified four filamentous fungi, Penicillium simpliciss...
In this study, the extraction yield, the mathematical modeling of pressurized liquid extraction (PLE) kinetics with sub- and supercritical carbon dioxide (SC-CO2) of olive leaves (Olea europaea) and the biological activity of the extracts were evaluated. The extraction with PLE was conducted isobarically (10.3 MPa), varying the temperature (20, 40...
The present study aims to use commercial antioxidants rosemary in different concentrations in mixed hamburger processing (beef, chicken, and turkey) and to evaluate its influence on the physical‐chemical, microbiological, sensory, and structural properties during shelf life. The results showed no significant difference for pH and acidity between th...
The main objective was to increase the applicability of palygorskite by palygorskite sheets using a tape casting method. The stability of the suspension was investigated and the tapes were characterized by TGA/DTA, XRD, and SEM‐FEG. The antimicrobial activity was analyzed in order to test the applicability of a newly modified drug release system th...
Neste trabalho, analisamos a diversidade e estrutura genética de diferentes populações de Ocotea odorifera em remanescentes florestais com diferentes condições edafoclimáticas, utilizando-se o marcador molecular RAPD (Random Amplified Polymorphic DNA) e marcador isoenzimático, visando fornecer informações para programas de conservação da espécie. O...
The aim of this study was to evaluate the action of commercial and non-commercial cellulase and pectinase on rice husk and Tifton 85 hay hydrolyses. The hydrolysis kinetics of the substrates with commercial cellulase and pectinase were evaluated and the hydrolysis at different temperature and agitation conditions was maximized using experimental de...
The aim of this work was to maximize the production and the partial characterization of carboxymethylcellulase (CMCase) and filter paper enzyme activity (FPase) using a new isolate by solid state fermentation with agro-industrial substrates and potential application of hydrolytic cellulosic substrates. Four isolates were initially evaluated, and th...
Cooling of carcasses using water immersion is the most used process in the poultry industry. In this stage, besides the reduction of temperature, water absorption in the carcasses occurs and, consequently, in the chicken cuts, which are monitored by the companies and supervised by the Ministry of Agriculture, Livestock, and Supply of Brazil, which...
Background
Liquid smokes treatment eliminated most problems associated to the traditional method, besides providing uniformity of flavour and colour in meat products.
Objective
This work evaluate the effects of liquid smoke treatment in the oxidative stability and content benzo(a)pyrenes, compared to the traditional smoking treatment in Sausage ty...
Different solvents and extraction solutions (distilled water, NaCl, Tween 80, citrate buffer, and acetate buffer) were evaluated for enzymes recovery. The independent variables evaluated were agitation (12–348 rpm), time (4.8–55.2 min), and temperature (13.2–46.8°C) and 10 extraction cycles, using an experimental design (Central composite rotatable...
The Italian-Type Sausage produced in Brazil is predominantly obtained from swine meat, with maturation approximately 30 days, reaching pH around 5.3. The use of Lactobacillus bacteria as starter cultures provide fermentation processes and products more uniform and safe, reducing the maturation time due to the rapid formation of lactic acid, obtaini...
The aim of this study was to evaluate the mycotoxicological quality of wheat flours used by bakeries from the North Region in Rio Grande do Sul state, Brazil, regarding the presence of mycotoxins. On collecting type-1 refined wheat flour, a conglomerate sampling from 13 cities and 3 bakeries per city (n = 39), selected from the defined region was p...
The present work introduces new functional bionanocomposite materials based on layered montmorillonite and fibrous sepiolite clays and two biopolymers (carboxymethylcellulose polysaccharide and zein protein) to produce drug-loaded bionanocomposite films for antibiotic topical delivery. Neomycin, an antibiotic indicated for wound infections, was emp...
This work aimed at studying methods for Listeria monocytogenes attached cells removal from food industry equipment. In order to verify possible contamination points, a tracking was carried out in food products and equipment surfaces (brine injection needles) by swab. Brine injection needles were defined as a study due to their higher contamination...
Xanthan gum, a biopolymer produced by the bacteria Xanthomonas campestris, with varied applications in many industrial branches. Cell immobilization is an alternative to enhance xanthan gum production, reducing both time and costs, while increasing yield. The present study sought to immobilize the microorganism Xanthomonas campestris in situ in pol...
This work aims at characterizing linseed oil obtained using different extraction methods (hexane, subcritical propane and pressurized ethanol), and comparing the results with commercial linseed oil extracted by cold mechanical press method. An experimental design helped to evaluate temperature and pressure effects on the oil extraction using propan...
The subtropical riverine forests present a variation in soil water availability throughout the year, following precipitation seasonality. The objective of this work was to evaluate the responses of Cordia americana to different light intensities combined with soil flooding. Seedlings were acclimated to light treatments, with full sun and shade cond...
This study aimed to evaluate the parameters that influence the water absorption and drip of chicken carcasses due to the processing and pre-cooling of the meat in an industrial chiller. A total of 1,179 chickens were sampled during industrial processing to evaluate the influence of variables, validate the parameters, and conduct histological analys...
This study reports the optimization of geranyl butanoate production by esterification of geraniol and butanoic acid in a solvent-free system using two immobilized lipases as catalyst. The operating conditions that optimized geranyl butanoate production were determined to be 40 °C, a geraniol to butanoic acid molar ratio of 3:1, 150 rpm, 5 wt% of en...
The sensory, physical-chemical and microbiological characteristics of boneless chicken thighs and drumsticks stored at different temperatures were evaluated. Samples stored at −6 and −12 °C, in natura and cooked, presented changes in sensory characteristics from the sixth and fifth month of storage, respectively. In natura samples kept at −18 °C pr...