Roger Fuoco

Roger Fuoco
Università di Pisa | UNIPI · Department of Chemistry and Industrial Chemistry

Full Professor of Analytical Chemistry

About

146
Publications
23,073
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,982
Citations
Citations since 2016
39 Research Items
1441 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
Additional affiliations
November 1994 - May 2017
Università di Pisa
Position
  • Professor (Full)

Publications

Publications (146)
Article
Full-text available
Heart failure (HF) is the main cause of mortality worldwide, particularly in the elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) is the gold standard biomarker for HF diagnosis and therapy monitoring. It is determined in blood samples by the immunochemical methods generally adopted by most laboratories. Saliva analysis is a powerful t...
Article
Full-text available
The aim of this study was to perform a systematic review on the potential value of saliva biomarkers in the diagnosis, management and prognosis of heart failure (HF). The correlation between saliva and plasma values of these biomarkers was also studied. PubMed was searched to collect relevant literature, i.e., case-control, cross-sectional studies...
Article
Full-text available
COVID-19 is a highly transmissible respiratory illness that has rapidly spread all over the world causing more than 115 million of cases and 2.5 million of deaths. Most epidemiological projections estimate that the SARS-CoV-2 virus causing the infection will circulate in the next few years and raise enormous economic and social issues. COVID-19 has...
Article
Breath analysis is an alternative approach for disease diagnosis and for monitoring therapy. The lack of standardized procedures for collecting and analysing breath samples currently limits its use in clinical practice. In order to overcome this limitation, the "Peppermint Consortium" was established within the breath community to carry out breath...
Article
Pollution from microplastics (MPs) has become one of the most relevant topics in environmental chemistry. The risks related to MPs include their capability to adsorb toxic and harmful molecular species, and to release additives and degradation products into ecosystems. Their role as a primary source of a broad range of harmful volatile organic comp...
Article
Full-text available
Heart failure (HF) is a cardiovascular disease affecting about 26 million people worldwide costing about $100 billons per year. HF activates several compensatory mechanisms and neurohormonal systems, so we hypothesized that the concomitant monitoring of a panel of potential biomarkers related to such conditions might help predicting HF evolution. S...
Article
Environmental pollution associated to plastic debris is gaining increasing relevance not only as a threat to ecosystems but also for its possible harmful effects on biota and human health. The release of toxic volatile organic compounds (VOCs) is a potential hazard associated with the environmental weathering of plastic debris. Artificial aging of...
Article
Biomarkers of oxidative stress are generally measured in blood and its derivatives. However, the invasiveness of blood collection makes the monitoring of such chemicals during exercise not feasible. Saliva analysis is an interesting approach in sport medicine because the collection procedure is easy-to-use and does not require specially-trained per...
Article
The continuous exchange of chemicals with blood and the non-invasive collection make saliva an interesting specimen for clinical applications, from the detection of biomarkers to the new –omic sciences in medicine. However, saliva sampling is challenging because the suitability of the collection method for the analyte of interest is either poorly i...
Article
This work presents a reliable analytical procedure combining micro-extraction by packed sorbent (MEPS) and ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry to determine 8-iso prostaglandin F2α, 8-iso prostaglandin E2 and prostaglandin E2 in dried blood spots (DBSs). To reach this goal, we optimized a fas...
Conference Paper
Exhaled air and breath condensate contain a large number of health biomarkers, such as volatile and semi-volatile organic compounds, proteins and lipids. Nowadays, the collection of breath samples is carried out by commercial or lab-made sampling systems that collect only one type of sample (e.g. gaseous or condensate phase), thus limiting the diag...
Article
Two third of our planet is covered by water, and lakes and rivers are the most important freshwater resources. But the amount of fresh water represents only 2.5% of the earth's water. Of the earth's fresh water, about 70% is locked up in the continental ice, 30% in underground aquifers and only 0.3% in rivers and lakes. In many water ecosystems aro...
Article
Full-text available
This paper deals with both the transport processes of several classes of organic pollutants in a large area of Victoria Land and Ross Sea (Antarctica) and the role played by marine aerosol which involves the exchange process between the sea surface and the atmosphere. The mechanisms of transfer process from the seawater to the atmosphere and aeroso...
Article
When working with humid gaseous samples, the amount of water vapour collected in a needle trap along with volatile analytes may vary from sample to sample and decrease during the storage. This has a major impact on desorption efficiency and recovery. We propose the addition of a labelled internal standards to nullify the effect of variable humidity...
Article
A reliable method for the determination of carbonyl compounds in exhaled breath based on on-sorbent derivatization coupled with thermal desorption and gas chromatography-tandem mass spectrometry is described. The analytical performances were optimized for a mixture of C2-C9 aldehydes and C3-C9 ketones, particularly interesting for clinical applicat...
Article
pH measuring and monitoring is fundamental to understand or control many chemical processes in biological, industrial or environmental fields. Potentiometric measurements by a glass electrode is the most common method to measure pH, although single-use paper strips are also widely used. Other methods include the use of hydrogen, quinhydron, and ant...
Poster
B-lines evaluated by lung ultrasound are a simple tool used to evaluate pulmonary congestion. Exhaled acetone concentration (EAC) reflects altered blood ketone body metabolism and increased acetone exhaust because of pulmonary congestion in heart failure (HF). Previous studies demonstrated a positive correlation of EAC and hemodynamic parameters in...
Article
Full-text available
The analytical performances of needle trap micro-extraction (NTME) coupled with gas chromatography tandem mass spectrometry were evaluated by analyzing a mixture of twenty-two representative breath VOCs belonging to different chemical classes (i.e. hydrocarbons, ketones, aldehydes, aromatics and sulfurs). NTME is an emerging technique that guarante...
Conference Paper
The aim of this work is to present a computational approach for the estimation of the severity of heart failure (HF) in terms of New York Heart Association (NYHA) class and the characterization of the status of the HF patients, during hospitalization, as acute, progressive or stable. The proposed method employs feature selection and classification...
Article
In the last decades, the possibility to assess physiological states, monitor disease onset and progression, as well as monitor post-treatment therapeutic outcomes through a non-invasive approach became one of the most desirable goals for healthcare research. In this respect, saliva analysis can represent a very interesting biological matrix which h...
Article
Nicotiana is a well characterized genus of Solanaceae. It is widely studied in literature in different research fields, such as biology, environmental chemistry, etc. In this study Nicotiana langsdorffii wilde type plants and transgenic for the rat glucocorticoid receptor gene (GR) and Agrobacterium rhizogenes rolC gene were used to value the effec...
Article
A method for the simultaneous determination of warfarin and its active metabolites (warfarin alcohols) in dried blood spots samples was developed and validated. The procedure consisted of the collection of blood sample spots on Whatman 903 filter paper and the liquid extraction of the analytes by a 3:1 v/v methanol/acetonitrile mixture from 6 mm di...
Article
The study was aimed to investigate the response in psoriatic patients of the two primary neuroendocrine systems, namely the Autonomic Nervous System and the Hypothalamic-Pituitary-Adrenal (HPA) axis, whose main biomarkers of activation are salivary α-amylase and cortisol, respectively. Reliable analytical procedures for the determination of salivar...
Article
This study was aimed at evaluating the influence of sampling procedure on the determination of uric acid and lactate in oral fluid. Samples of non-stimulated and stimulated oral fluid were collected from 22 healthy volunteers. Different frequencies of stimulation were obtained by moving a polyester swab within the mouth at 50, 100 and 150 min− 1. T...
Article
The aim of this study was to investigate the relationship between warfarin dosage, international normalized ratio, plasma and oral fluid concentrations of warfarin, and its metabolites, namely RR/SS- and RS/SR-warfarin alcohols. Nine patients on long-term warfarin therapy (4 with stable and 5 with unstable international normalized ratio values) wer...
Article
Point-of-care applications and patients’ real-time monitoring outside a clinical setting would require disposable and durable sensors to provide better therapies and quality of life for patients. This paper describes the fabrication and performances of a temperature and a pH sensor on a biocompatible and wearable board for healthcare applications....
Article
In this paper we investigated the presence of Polycyclic Aromatic Hydrocarbons and Polychlorobiphenyls in a 50-m deep snow/firn core collected at the peripheral site GV7 in East Antarctica during the 2013–2014 XXIX Italian expedition. The concentration depth profile was obtained on the basis of the total concentration of fourteen PAHs and seven PCB...
Conference Paper
This article describes the fabrication and characterization of a pH sensor for monitoring the wound status. The pH sensitive layer consists of a graphene oxide (GO) layer obtained by drop-casting 5 μL of GO dispersion onto the working electrode of a screen-printed substrate. Sensitivity was 31.8 mV/pH with an accuracy of 0.3 unit of pH. Open-circui...
Conference Paper
Full-text available
This work shows how saliva and breath analysis can be used for the non-invasive monitoring of drugs and patients' health status. Saliva was sampled to determine both warfarin, an anti-coagulant that is presently measured in blood by expensive and invasive procedures, and several biomarkers possibly related to heart failure. Breath analysis was used...
Article
Nalophan, Tedlar and Cali-5-Bond polymeric bags were compared to determine the most suitable type for breath sampling and storage when volatile organic compounds are to be determined. Analyses were performed by thermal desorption gas chromatography mass spectrometry. For each bag, the release of contaminants and the chemical stability of a gaseous...
Conference Paper
Full-text available
Heart Failure is a rapidly increasing cardiovascular chronic disease that affects millions of people globally. Lack of proper management of HF patients increases the risk of frailty and other undesirable effects and contributes to loss of independence. The engagement of the HF patient and all actors related to his/her disease management, including...
Conference Paper
This work presents a computerized system to monitor mouth pressure, tidal volume, exhaled airflow, respiration rate and end-tidal partial pressure of CO2 during breath collection. The system was used to investigate the effect of different respiratory rates on the volatile organic compounds (VOCs) concentrations in exhaled breath. For this purpose,...
Article
Full-text available
The determination of warfarin, RS/SR- and RR/SS-warfarin alcohols in oral fluid may offer additional information to the INR assay. This study aimed to establish an optimized sampling technique providing the best correlation between the oral fluid and the unbound plasma concentrations of these compounds. Samples of non-stimulated and stimulated oral...
Article
Background Ultraviolet (UV) absorbance of spent dialysate has been proposed as a method for monitoring hemodialysis efficiency. The contribution of the various uremic toxins to UV absorption, however, needs clarifying. Methods Urea, creatinine and uric acid were measured in blood and dialysate before and during dialysis in 22 maintenance hemodialy...
Conference Paper
Methods for the determination of warfarin and warfarin alcohols in plasma and oral fluid were developed as a first step towards a minimally invasive monitoring of warfarin therapy and to clarify the role of warfarin alcohols in the anticoagulation process. Warfarin is the most common anticoagulant drug prescribed for the treatment of many diseases...
Conference Paper
Methods for the determination of warfarin and warfarin alcohols in oral fluid and plasma were developed in order to establish the best oral fluid sampling that provide the highest correlation between the oral fluid and unbound plasmatic concentration of warfarin and warfarin alcohols. Warfarin is essential and not replaceable in a large number of c...
Conference Paper
An HPLC method with spectrofluorimetric detection is presented for the determination of warfarin (WAR) and some of its metabolites (warfarin alcohols, WAROHs) in saliva and human plasma (total content and fraction not bound to albumin). The aim of research is to develop a minimally invasive monitoring technique for this oral anticoagulant and clari...
Article
Full-text available
Sevoflurane (SEV), a commonly used anesthetic agent for invasive surgery, is directly eliminated via exhaled breath and indirectly by metabolic conversion to inorganic fluoride and hexafluoroisopropanol (HFIP), which is also eliminated in the breath. We studied the post-operative elimination of SEV and HFIP of six patients that had undergone a vari...
Article
Full-text available
The evolution of breath composition during oral glucose tolerance tests (OGTTs) was analysed by thermal desorption/gas chromatography/mass spectrometry in 16 subjects and correlated to blood glucose levels. The glucose tolerance tests classified five of the subjects as diabetics, eight as affected by impaired glucose tolerance and three as normogly...
Article
Full-text available
Recently our findings have shown that the integration of the gene coding for the rat gluco-corticoid receptor (GR receptor) in Nicotiana langsdorffii plants induced morphophysiological effects in transgenic plants through the modification of their hormonal pattern. Phytohormones play a key role in plant responses to many different biotic and abioti...
Conference Paper
Background Warfarin is an essential and not replaceable drug in a large number of clinical conditions. Therapeutic Drug Monitoring (TDM) is necessary because several factors may interact with the therapy due to the narrow therapeutic range of the drug. Saliva is suitable for TDM because the salivary concentration only mirrors the free plasma concen...
Conference Paper
Warfarin is the most used anticoagulant for the prevention of thrombosis and thromboembolism. It is a weakly acid drug (pKa = 5.19, at 25 °C), highly bound to plasma albumin (> 99%). Only the unbound fraction can carry out a therapeutic action and cross the biological membranes (e.g. salivary glands). The drug is metabolized by the CYP450 syste...
Chapter
IntroductionSampling Methods Analytical TechniquesApplication of Breath AnalysisExposure AssessmentExhaled Breath CondensateConclusions References
Article
A four-century record of fourteen polycyclic aromatic hydrocarbons (PAHs) and seven polychlorobiphenyls (PCBs) was obtained from two snow/firn cores gathered at Talos Dome (Antarctica) in order to assess the con-tribution of human activities to global environmental pollution. The total concentrations of ΣPAH 14 and ΣPCB 7 were 0.7 ng/l and 0.06 ng/...
Data
Distribution of pH values in patient oral fluid samples. (TIF)
Data
Chromatograms of representative sweat samples. A) a blank sweat sample from a volunteer not taking the drug; B) a patient sweat sample with an estimated warfarin concentration of about 4 ng/mL (tr = 10.2 min). (TIF)
Article
Full-text available
Patients on warfarin therapy undergo invasive and expensive checks for the coagulability of their blood. No information on coagulation levels is currently available between two controls. A method was developed to determine warfarin in oral fluid by HPLC and fluorimetric detection. The chromatographic separation was performed at room temperature on...
Conference Paper
Oral anticoagulants are essential and not replaceable in a large number of long- lasting clinical conditions which require an accurate control of the coagulation of blood, such as thrombotic diseases and vascular pathologies. Oral anticoagulants have such a narrow therapeutic index that small changes in plasma concentration may have serious consequ...
Article
Microcystins (MCs) are dangerous toxins produced by cyanobacteria in eutrophic waters, that are increasingly used worldwide for human consumption after potabilization. In this study, we present the results of laboratory and pilot-plant experiments, aimed at deepening the knowledge of the mechanisms governing the equilibrium of dissolved and particu...