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Publications (22)
Agrobacterium-mediated transformation and particle bombardment are the two common approaches for genome editing in plant species using CRISPR/Cas9 system. Both methods require careful manipulations of undifferentiated cells and tissue culture to regenerate the potentially edited plants. However, tissue culture techniques are laborious and time-cons...
Malaysia was one of the top producers and exporters of papaya (Carica papaya L.) in the world prior to the major collapse of the industry due to papaya dieback disease. Erwinia mallotivora has been identified as the pathogen causing the disease and in order to revitalise the papaya industry, both conventional and genetic engineering approaches have...
Callogenesis and embryogenesis are integral parts of many tissue culture procedures for genetic manipulation in rice. However, the efficiency of both processes is largely dependent on the media constituent especially the plant growth regulators (PGRs) due to the genotype-dependent nature of in vitro culture protocols. Therefore, this study investig...
Abstract: Callogenesis and embryogenesis are integral parts of many tissue culture procedures for genetic manipulation in rice. However, the efficiency of both processes is largely dependent on the media constituent especially the plant growth regulators (PGRs) due to the genotype-dependent nature of in vitro culture protocols. Therefore, this stud...
Sheath blight disease is one of the major rice diseases caused by a soilborne pathogenic fungus known as Rhizoctonia solani and the search for resistant rice varieties has been extensively conducted in the conventional breeding programme. As an alternative approach, genetic engineering has been employed to develop transgenic rice with increased res...
Pluronic F-68 (PF-68) is a non-ionic surfactant used in plant tissue culture as a growth additive. Despite its usage as a plant growth enhancer, the mechanism underlying the growth-promoting effects of PF-68 remains largely unknown. Hence, this study was undertaken to elucidate the growth-promoting mechanism of PF-68 using recalcitrant MR 219 callu...
This study was undertaken to evaluate growth-promoting effects of Pluronic F-68 (PF-68) on recalcitrant MR 219 rice callus. Our study shows that calli grown on Murashige and Skoog medium supplemented with 0.04% PF-68 significantly increased callus proliferation by 58.80% (fresh weight) and 23.98% (dry weight) while root formation from callus was en...
Heterotrigona itama is a species of stingless bee recently domesticated (or reared) for honey production in a few Southeast Asian countries namely Malaysia and Indonesia. Being categorized in the clade Corbiculata together with the honeybees (Apis spp.) and bumble bees (Bombus spp.), the stingless bees are highly social in which the colony members...
The main objective of this study was to develop transgenic Malaysian Eksotika papaya with enhanced resistance to papaya dieback disease (PDD). Papaya dieback disease, which is caused by a Gram-negative bacterium, Erwinia mallotivora, has adversely affected Malaysia papaya industry and resulted in major production declines and economical losses. To...
In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood in...
Carica papaya L. or commonly known as papaya, is a major tropical crop consumed worldwide either as a vegetable or fresh fruit or processed products. In Malaysia, papaya was initially planted as a smallholder crop throughout the country. Eventually after 15 years of breeding and selection, a new variety, named C. papaya L. var. Eksotika, was releas...
Eksotika papaya (Carica papaya L. cv. Eksotika) is one of the important varieties grown in Malaysia. However, the industry is facing a threat of papaya dieback disease which has been adversely affected the domestic papaya production and supply. Therefore, to control the disease, an alternative strategy to disrupt the communication or quorum sensing...
Pecah Beling (Strobilanthes crispus) is a shrub that is often used in traditional medicine. It contains various essential bioactives such as phenolics, flavonoids and terpenoids, that contributes to its biological activities such as anticancer, antidiabetic and anti-inflammatory properties. Due to the potential this plant possesses in various healt...
In this study, we attempted to genetically modify Arabidopsis thaliana to increase its resistance against a bacterial infection using lactonase genes isolated from local bacterial isolates. Arabidopsis thaliana was chosen to be the target organism for genetic modification because the transformation approach of this model plant is relatively simple...
Graphical abstract Abstract The main objective of this study is to develop marker-free transgenic papaya plants via positive selection using phosphomannose isomerase (pmi) gene. Phosphomannose isomerase (PMI) is an enzyme that converts mannose-6-phosphate to fructose-6-phosphate, a glycolysis intermediate that supports the growth of plant cells. To...
The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearin...
The Malaysian Eksotika papaya (Carica papaya L.) has poor keeping quality due to its fast ripening attribute, which leads to post-harvest losses. This study is aimed at extending the shelf life of this perishable Eksotika fruit using antisense technology. A total of 6,000 Eksotika somatic embryogenic calli was transformed with the antisense 1-amino...
A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryo...
Chitinase catalyses the hydrolysis of β-1,4-N-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin and is involved in the inducible defenses of plants. The aim of this research was to isolate and clone chitinase cDNAs from the seed of winged bean. Chitinase gene fragments were isolated from a winged bean seed cDNA library using two...