Rodrigo Rezende

• PhD, MSc in Chemical Engineering
• Researcher at Centro de Tecnologia da Informação Renato Archer

Background: Hyaluronic acid (HA) is a natural polymer widely used as a vehicle in injectable cell therapy for the treatment of arthropathies. Objective: To estimate, through computational simulations and in vitro validation, the influence of HA’s physicochemical properties and administration speed on the shear stress generated in the syringe/needle...

Com base no conceito de economia circular, resíduos de fontes vegetais, entre eles manga, são reaproveitados e podem ser extraídas algumas biomoléculas como pectina e celulose para serem utilizadas em aplicações biotecnológicas, também para a indústria cosmética, até mesmo como biomateriais. Neste contexto, foi realizada a caracterização térmica, m...

O Instituto de Pesquisa com Células-tronco (IPCT) oferece o Curso de Medicina Regenerativa Beneficente (SOS RS). O curso, de 12 horas de duração, será realizado de 06 à 08 de Junho no formato online e conta com a participação de renomados profissionais, do Brasil e exterior. Ao inscrever-se no curso você, além de adquirir conhecimento científico,...

O 2nd International Digital Congress on 3D Biofabrication and Bioprinting (3DBB) é um evento Científico de Pesquisa e Inovação Tecnológica, sendo esta edição realizada por uma iniciativa do Programa de Pós-Graduação em Biotecnologia da Universidade de Araraquara – Uniara, com coorganização do Núcleo de Tecnologias Estratégias em Saúde da Universida...

Photobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured in standard, two-dimensional conditions are well established. Conversely, the characteristics of this effect in three-dimensional (3D) cultures, which are currently recommended due to the greater similarity with cellular behavior in vivo, have not yet been widely i...

Three-dimensional cell culture has tremendous advantages to closely mimic the in vivo architecture and microenvironment of healthy tissue and organs, as well as of solid tumors. Spheroids are currently the most attractive 3D model to produce uniform reproducible cell structures as well as a potential basis for engineering large tissues and complex...

Biofabrication is an incipient and fast-growing field of research that continues to develop groundbreaking innovations. Considerable progress has also been made in additive manufacturing technologies, which are used for the production of complex geometry using biomaterials, such as scaffolds and, most recently, bioink-based structures. Some of the...

Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo . The biomodulatory effect of low‐intensity la...

In recent research developed by our group, it was characterized and described the technical acquisition parameters of magnetic resonance imaging (MRI), with magnetic field intensity (MFi) of 1.5T, and its processing for printing three‐dimensional (3D) models of articular cartilage of patella. Such methodology should significantly contribute to the...

Polyetheretherketone (PEEK) has been prominent in orthopedic implants; however, it is inert, preventing interaction between the implant and adjacent bone tissues. One way to overcome this characteristic is physical modification its on surface by particle leaching promoting greater osseointegration. The objective of this research was to develop and...

Abstract Computer Simulation for biological and bioengineering purposes is being referred today also as In silico as a first approach for the In Vitro and In Vivo expensive and time-consuming tests. It is a necessary and fast-growing field of interdisciplinary knowledge to fulfill today's and future demands, ranging from bioengineering modeling and...

This review proposes to present how materials at nanolevel scale can contribute to the development of three-dimensional (3D) structures, human tissues, and organs which have macrolevel organization. Specific nanomaterials such as nanofibers and nanoparticles are presented and discussed in their application for biofabricating 3D human tissues and or...

The purpose of this work is to present different approaches to predict the development and behavior of several biological processes, such as molecular networks, gene interactions, diffusion, cell differentiation, tissue and organ development, beyond to provide new perspectives and strategies in the biofabrication of tissues and organs. Results and...

Statement of Purpose:3D bioprinting process can be adapted to produce tissues in a variety of formats, structural complexities, such as material types, cell types, growth factors and differentiation, extracellular matrix composition, mechanical properties, macro and microvasculatureand technical challenges associated with the creation of biomodelst...

A very complex design (blueprint) will be fundamental to reach a 3D bioprinted organ in the future. Since many biological and mechanical aspects are involved the project of an organ encompasses an over wide range of variables. In vitro and in vivo experiments require the investment of large sums of money and time, besides being specific and complex

O que é Biofabricação de órgãos? A construção automatizada de produtos biologicamente funcionais com organização estrutural de células vivas, biomoléculas, biomateriais e agregados celulares, por meio da bioimpressão 3D e subsequente maturação em biorreatores. Objetivos • Produzir microtecidos e organoides para testes e desenvolvimento de drogas; •...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach, has been practiced in several fields, and offers new opportunities for medical investigation and discovery, help in and improving the generation of new data and the storage, organization, and classification...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach has been prac­ticed in several fields, and offers new opportunities for medical investigation and discovery, helping and improving the generation of new data and the storage, organization, and classification...

Este trabalho propõe um blueprint interdisciplinar para a biofabricação de órgãos que utiliza as ideias comuns de simulações - como métodos probabilísticos preditivos e cálculos de energia - aplicados a dois frameworks: o mecânico e o biológico. Em resumo, o mecânico utilizaria a análise de elementos finitos para observar comportamentos fluidodinâ...

The biofabrication line comprehends mainly of the following equipment: cell sorters, microfluidic devices, tissue spheroid fabricator and encapsulator, bioprinter, bioreactor among others, as can be seen in this video. YpuTube --> https://www.youtube.com/watch?v=-xPAcX5yVgk&list=PLs_Es7-NwvIwlyt0T0ueJFJE_hJIcZj6O

The bioprinting step is an emerging variant of the biomedical application of additive manufacturing or 3D printing. This step depends on the other aspects, such as the design of the organ (BioCAD) and the biomaterial for processing. YouTube --> https://www.youtube.com/watch?v=ok5EjiFZ5_Q&list=PLs_Es7-NwvIzpxcrHw3VprEzMQQZalVoB&index=3 - http://www...

Introduction: Nowadays, 3D bioprinting processes can be used to produce biocompatible products in a variety of formats, structural complexities, based on different properties and materials, cell types, growth factors, and differentiation stage. Moreover, the extracellular matrix composition, mechanical properties, macro and microvasculature, and th...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach has been prac­ticed in several fields, and offers new opportunities for medical investigation and discovery, helping and improving the generation of new data and the storage, organization, and classification...

Materials, such as biopolymers, can be applied to produce scaffolds as mechanical support for cell growth in regenerative medicine. Two examples are polycaprolactone (PCL) and poly (lactic-coglycolic acid) (PLGA), both used in this study to evaluate the behavior of umbilical cord-derived mesenchymal stem cells. The scaffolds were produced by the 3D...

Biofabrication as an interdisciplinary area is fostering new knowledge and integration of areas like nanotechnology, chemistry, biology, physics, materials science, control systems, among many others, necessary to accomplish the challenge of bioengineering functional complex tissues. The emergence of integrated platforms and systems biology to unde...

La fabricación por manufactura aditiva (MA) ha sido investigada y desarrollada desde los últimos 20 años. Esto ha conllevado también al avance de otras áreas como la ingeniería de tejidos. La novedad y la ventaja de estas técnicas recaen en el hecho de que en lugar de fundamentar el proceso en la eliminación de material, ésta crea piezas tridimensi...

Review Information technology (IT) is ubiquitous in recent human existence. The aim of this article is to present some basic concepts and specific demands that biofabrication may place on IT. Some of these technologies are already available, with a need for improvement, while others will need to be newly developed. Technologies that clearly, precis...

Technological advances in medical imaging have provided healthcare professionals with powerful resources for storing, analyzing, and visualizing three-dimensional images in a variety of diagnostic tasks. Equipments for acquiring high-quality images and computer-aided tools for image interpretation play an important role in surgical planning, diseas...

Organ printing is defined as a layer-by-layer, additive, robotic, and computer-aided biofabrication of functional three-dimensional (3D) organ constructs using self-assembling tissue spheroids according to a digital model. Information technology and computer-aided design software are instrumental in the transformation of virtual 3D bioimaging infor...

Adipose stem cells (ASCs) spheroids show enhanced regenerative effects compared to single cells. Also, spheroids have been recently introduced as building blocks in directed self-assembly strategy. Recent efforts aim to improve long-term cell retention and integration by the use of microencapsulation delivery systems that can rapidly integrate in t...

Aggregates of cells form structures called tissue spheroids (TS) that have been widely used in the field of tissue engineering. The contact of the cells with the culture medium is related to cell viability and to the increase of proliferation rate. Nutrition, organization and growth in cells are largely determined by diffusion mechanisms. Due to th...

Cardiovascular disease remains as one of the main problems in contemporary health care worldwide. Several studies of the cardiac prostheses have been held since 60s with the advent of cardiopulmonary bypass. The mechanical properties of blood vessels, arteries and valves depend on collagen and elastic fibers, as well as on smooth muscle cells and g...

Nutrition, organization, growth and signal transduction in cells are largely determined by diffusion mechanisms. The complex three-dimensional shapes of cellular environment complicate the experimental analysis and computational simulation of diffusion in live cells. Three-dimensional cell aggregates are called tissue spheroids and they are widely...

Tissue spheroids (chondrospheres) are already used in clinical practice for treatment of cartilage defects. However, potential washing out of some non-attached tissue spheroids strongly suggests that preliminary attachment and spreading of tissue spheroids on electrospun matrices could potentially eliminate this undesirable effect. In order to esti...

The main goal of this proposal was the development of a 3D design/model for computer simulation of a heart valve and its comparison with a valve made by polyurethane (PU) in silico.

Introduction: Tissue engineering is a field which is currently under a great deal of investigation for the development and/or restoration of tissue and organs, through the combination of cell therapy with biomaterials. Rapid prototyping or additive manufacturing is a versatile technology which makes possible the fabrication of three dimensional (3...

About 30 years ago, the 3D printing technique appeared. From that time on, engineers in medical science field started to look at 3D printing as a partner. Firstly, biocompatible and biodegradable 3D structures for cell seeding called "scaffolds" were fabricated for in vitro and in vivo animal trials. The advances proved to be of great importance, b...

Tissue spheroids (chondrospheres) are already used in clinical practice for treatment of cartilage defects. However, potential washing out of some non-attached tissue spheroids strongly suggests that preliminary attachment and spreading of tissue spheroids on electrospun matrices could potentially eliminate this undesarable effect. In order to esti...

Organ printing is defined as a layer by layer additive robotic computer-aided biofabrication of functional 3D organ constructs with using self-assembling tissue spheroids according to digital model. Information technology and computer-aided design softwares are instrumental in the transformation of virtual 3D bioimaging information about human tiss...

The ultimate goal of bioprinting is the production of living and functional tissue and organs for transplantation in a reasonable time scale. To achieve this goal, the best process would be organ printing. It enables creation of tissue with a high level of cell density; can solve the problem of vascularization in thick tissues; organ printing can b...

Recent advances in additive manufacturing have allowed, even in a preliminary stage, 3D printing of biomaterials and/or cells. Each day, biomanufacturing process has been adapted to produce, in the future, different kinds of human tissues in a wide variety of shapes and structural complexities, which have specific biomechanical properties such as t...

Organ printing is a main challenge of rapidly emerging 3D bioprinting technology. The bioprinted organ must be i) bioprinted according to specially developed digital model; ii) consist of authentic and histotypical structural-functional units of desirable organ; iii) well vascularized before implantation into recipient organism; iv) structurally in...

About 30 years ago, the 3D printing technique appeared. From that time on, engineers in medical science field started to look at 3D printing as a partner. First, biocompatible and biodegradable 3D structures for cell seeding called “scaffolds” were fabricated for in vitro and in vivo animal trials. The advances proved to be of great importance, but...

Biopolímeros podem ser usados na produção de matrizes como suporte mecânico no crescimento celular na medicina regenerativa. Dois exemplos são a policaprolactona (PCL) e o poli(ácido láctico-co-ácido glicólico) (PLGA) usados no presente estudo para avaliar as células-tronco mesenquimais em matrizes de PCL produzidos por impressão 3D e cobertos com...

The study of how cells interact and the principles of biological self-assembly during the development of 3D tissue engineered constructs are very important during the biofabrication of organs. Several multicellular spheroids or cellular aggregates recovered by hydrogel (bio-ink particles) have been used as ‘building blocks’ for tissue and organ pri...

Aggregates of pre-sorted cells form structures called tissue spheroids that have been widely used in the field of tissue engineering. The greater contact of the cells with the culture medium is directly related to cell viability and to the increase of proliferation rate. Due to the characteristics of a 3D environment, at some zones within the tissu...

The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic–inorganic zirconium silicate. Their mechanical properties are theoretically modeled using finite elements analysis...

Organ printing or Bioprinting is a 10 years-old technology. It is an interdisciplinary field involving many sciences and technologies among life sciences, biology, computer sciences, physics and engineering. The modern world mainly at urban areas has generated a high number of patients waiting for organs because of diseases, congenital problems and...

Tissue Engineering, is also known as a field of Regenerative Medicine. Condrocytes 3D culture is necessary to obtain a sufficient number of cells for cartilage bioengineered in Bioprinter system. However, the current evidences demonstrate that cellular microenvironment direct cell behavior through influences in signaling pathways. Cells behave more...

Organ printing technology could be defined as an automated, robotic and computer-aided layer by layer additive biofabrication of functional 3D tissue and organ constructs using living tissue spheroids as building blocks. The concept of organ printing has been introduced a decade ago as a potentially superior alternative to conventional solid scaffo...

Thermosensitive interpenetrating gels were prepared by physically blending poly(N-isopropylacrylamide) (PNIPA) as the matrix and the following polysaccharides as interpenetrating phases: chitosan oligosaccharides (identified as QNAD and QNED) and soluble starch (STARCH). The molecular weight of the dispersed phase, the free water/bound water ratio...

Organ printing technology or robotic additive biofabrication of 3D functional tissue and organ constructs is based on using tissue spheroids as building blocks. In order to bioprint human organs it is necessary to develop technology for scalable production of millions tissue spheroids. Ideally, these tissue spheroids must have standard size and sha...

Organ printing is a variant of the biomedical application of additive manufacturing (rapid prototyping) technology or layer-by-layer additive biofabrication of 3D tissue and organ constructs using self-assembled tissue spheroids as building blocks. Bioengineering of perfusable intraorgan branched vascular trees incorporated into 3D tissue construct...

The first part of this review was published in Biomedical Engineering, No. 3, 2013. This second part discusses development and application of tissue spheroid encapsulators, robotics bioprinters, bioreactors, and problems of computer design of biofabrication lines.

Polylactic (PLA), Polycaprolactone (PCL) and blend of Polylactid/ Polycaprolactone (PLA/PCL) scaffolds constructs composed by layers of microsized filaments (0/90° lay-down pattern), with a diameter of around 400μm and interfilament distance of around 200 μm and 10 layers, were produced using a melt extrusion-based additive manufacturing technique....

The growing interest in tissue engineering has stimulated the research of biomaterials that can be used as cellular supports and/or scaffolds to subsequently stimulate and/or regenerate tissues. Based on this premise, biodegradable polyesters: amorphous Poly (Lactic-acid) (PLA) and semi-crystalline Poly(-caprolactone) (PCL), were used for manufact...

Organ printing is defined as a computer-aided layer-by-layer additive robotic biofabrication of functional human 3D tissue and organ constructs using self-assembling tissue spheroids as building blocks. During last decade organ printing has rapidly emerged as a potentially superior alternative to the classic solid scaffold-based approach in tissue...

Bioprinting of tissues and organs can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks. The microtissues and tissue spheroids are living materials with certain measurable, evolving and potentially controllable composition,...

Directed tissue self-assembly or bottom-up modular approach in tissue biofabrication is an attractive and potentially superior alternative to a classic top-down solid scaffold-based approach in tissue engineering. For example, rapidly emerging organ printing technology using self-assembling tissue spheroids as building blocks is enabling computer-a...

Nanotechnology is a rapidly emerging technology dealing with so-called nanomaterials which at least in one dimension have size smaller than 100 nm. One of the most potentially promising applications of nanotechnology is in the area of tissue engineering, including biofabrication of 3D human tissues and organs. This paper focused on demonstrating ho...

Organ printing is defined as the layer by layer additive biofabrication of three-dimensional (3D) tissue and organ constructs using tissue spheroids as building blocks. Ultimately, successful bioprinting of human organ constructs is dependent on a 'built in' vascular tree to perfuse and maintain the viability of the organ constructs. Thus, the desi...