Rodolfo Luzbel de la Sota

• MV, MSc, PhD, Diplom ECAR
• Professor (Full) at National University of La Plata

Aquaporins are specific water channels that mediate the movement of water and cryoprotectants across the spermatozoa plasma membrane. The aim was to assess the presence and location of AQP1, AQP7 and AQP11 in fresh and frozen–thawed canine epididymal sperm. Using immunocytochemistry, aquaporins 1, 7 and 11 were detected in fresh and frozen spermato...

Estrogen-based protocols are commonly combined with a progestin-releasing intravaginal device (P4RID) and prostaglandin (PG) to synchronize follicle wave emergence before AI in Bos taurus cattle, with pregnancies per AI (P/AI) ranging from 40% to 55%. Gonadotrophin-releasing hormone (GnRH)-based protocols + P4RID + PG rely on the presence of a phys...

La resistencia antimicrobiana (RAM) representa uno de los mayores desafíos de salud del siglo XXI, por lo que su monitoreo en los entornos productivos es fundamental para entender y controlar su propagación. La metritis es una enfermedad grave del posparto que afecta el bienestar y la rentabilidad del tambo y que usualmente requiere tratamiento ant...

Antimicrobial resistance (AMR) is a critical public health challenge. AMR surveillance in production environments is crucial, as they can serve as reservoirs for resistant pathogens. Metritis is a postpartum disease that usually requires antibiotic treatment and affects animal welfare and profitability. Our study used whole genome sequencing to com...

Una respuesta inmunológica sólida después del parto es fundamental para la futura fertilidad de la vaca, ya que permite la eliminación de bacterias potencialmente dañinas del útero. Las vacas con un sistema inmunológico comprometido pueden tener una mayor susceptibilidad a desarrollar infecciones posparto. Nuestra hipótesis se basó en que la evalua...

The aim was to determine changes in clinical parameters, glucose concentration, cortisol and behavior in colony queens in no music conditions compared with exposing to different genres of music. Mixed breed clinically healthy queens ( N = 9) were used. Queens were studied under no music conditions (control=CON) and auditory enrichment: Soft Rock (M...

The aim of this study was to know the opinion of citizens about castration as a preventive practice of hormone-dependent reproductive diseases and population control. A questionnaire was developed through a Google form and distributed online to residents of the city of La Plata, Argentina, owning at least one pet. The questionnaire included multipl...

Due to the excessive use of antimicrobial drugs, many multidrug-resistant (MDR) bacteria have emerged and spread, threatening global health. Calf respiratory disease is common during calfhood, and its treatment often consists of administering antibiotics. Fluoroquinolones (FQ), a class of antibiotics critically important for human health, are used...

This study aimed to describe the antimicrobial resistance (AMR) genes of quinolone-resistant Escherichia coli isolated from dairy calves and their environment. Six dairy farms from Buenos Aires, Argentina, were enrolled. Samples were taken by rectal swabbing from healthy, untreated calves at different ages (T1= calves ≤3 days old, n= 69; T2= calves...

Control measures are being introduced globally to reduce the prevalence of antibiotic resistance (ABR) in bacteria on farms. However, little is known about the current prevalence and molecular ecology of ABR in bacterial species with the potential to be key opportunistic human pathogens, such as Escherichia coli, on South American farms. Working wi...

Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia Inhibitory Factor [LIF]), and Progesterone Receptor (PR) in clinically healthy bitches with a normal endometrium and with endometritis, without the presence of cystic endometrial hyperplasia. Forty-eight m...

The relationship between cervicitis and fertility has been poorly studied in dairy cows. Endocervical inflammation, determined by an increase of polymorphonuclear leukocytes (PMN), is linked with lower hazard of pregnancy. The objective of the present study was to evaluate if an increase in PMN in the cervical mucosa is associated with decreased fe...

A retrospective longitudinal study assessing the explanatory and predictive capacity of body condition score (BCS) in dairy cows on disease risk at the individual and herd level was carried out. Data from two commercial grazing herds from the Argentinean Pampa were gathered (Herd A = 2100 and herd B = 2600 milking cows per year) for 4 years. Logist...

La resistencia antimicrobiana (RAM) es un problema mundial que afecta la salud animal, humana y ambiental, sin embargo, la información sobre RAM en crianza de terneros en tambos es escasa. El objetivo del estudio fue describir la RAM en E. coli aislada de terneros Holando Argentino sanos y su entorno a lo largo de un año. Se visitaron seis tambos d...

Control measures are being introduced globally to reduce the prevalence of antibiotic resistant (ABR) bacteria on farms. However, little is known about the current prevalence and molecular ecology of ABR in key opportunistic human pathogens such as Escherichia coli on South American farms. Working with 30 dairy cattle farms and 40 pig farms across...

Introduction: Food animals are important reservoirs of antimicrobial resistant (AMR) bacteria. However, most AMR studies focused on poultry, swine, or feedlots production systems, with scarce attention paid to calf production. This study describes the proportion of resistant Escherichia coli (E. coli) isolated from healthy dairy calves of different...

Objective: To compare the capacity of a simplified calf health scoring chart (SIM score) with the University of Wisconsin's calf health scoring chart (WIN score) for the diagnosis of calf diarrhea and calf respiratory disease (RD). Animals and procedures: Holstein calves (N = 222) were clinically evaluated for diarrhea and RD diagnosis using the...

Intro Argentina is one of South America's leading milk and pork producers, yet the data for antimicrobial usage and resistance in these sectors is extremely limited. Here we report third-generation cephalosporin resistance (3GC-R) rates among E. coli from pigs and dairy cattle in two Argentinian regions (La Plata and Rio Cuarto) and the resistance...

The endometrium is the uterine layer wherein embryo implantation and development take place. Complete knowledge of the endometrium’s normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species, but the cause/s remains unknown. Furthermo...

The domestic cat is a highly prolific species; thus, reproductive control is crucial to reducing feral cat overpopulation. This study aimed to assess the effect of a commercially-available GnRH vaccine for swine on suppressing sperm production in male cats. Twelve sexually mature tomcats were randomly divided into two groups. Treated cats (n = 9) r...

The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultras...

Objectives: To determine antimicrobial (AM) resistance patterns of E. coli from dairy calves and their environment during the rearing period. Materials and Methods: Six commercial dairy farms located in Buenos Aires, Argentina, were each visited twice, once during winter (June-August 2021) and once during spring (September-December 2021). Rectal sw...

Objective: The aim was to determine the vertical and horizontal transmission of Mycobacterium avium subsp. paratuberculosis (Map), Bovine viral diarrhea virus (BVDV), Bovine herpes virus 1 (BHV1), and Neospora caninum (N caninum) in a dairy herd Materials and methods: This study was conducted in a commercial dairy farm located in Castelli, Buenos A...

The endometrium is the uterine layer wherein embryo implantation and development take place. Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species but the cause/s remain unknown. Furthermore...

The main aim of this study was to determine the antimicrobial resistance patterns of Escherichia coli isolated from dairy calves of different ages across winter, spring, summer and autumn. For this, six commercial dairy farms located in Buenos Aires, Argentina, were visited once per season. Rectal swabs were obtained from healthy calves (n=286) at...

Many studies have been conducted to estimate pregnancy losses between 19 and 34 d after artificial insemination (AI) in dairy cows managed under confinement-based systems, but few studies have examined embryo mortality during this interval in dairy cows managed under gazing systems. The objectives of this prospective cohort study were (1) to assess...

The endometrium is a uterine layer essential for achieving optimal embryo implantation and development. Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species; however, the cause is unknown,...

This study aimed to assess 1) the effect of high environmental temperatures on sperm production and 2) the effectiveness of a temperature-humidity index (THI) to predict the degree of thermal stress in a cat model. Semen collection was performed by electroejaculation for 18 mo in 20 tomcats maintained under controlled photoperiod. Still, temperatur...

Poor udder health status can have a detrimental effect on milk yield and reproductive performance, leading to reductions in the dairy farm profit. The objective of this retrospective longitudinal study was to assess the associations of somatic cell count (SCC) with daily milk yield and reproductive performance. A database with 1,930,376 lactations...

This work aimed to study the immunolocalization of vascular endothelial growth factor (VEGF) and its FLT‐1 receptor (VEGFR1) in systemically healthy bitches with and without endometritis (END). Thirty‐one uterine samples from mixed‐breed, systemically healthy diestrus bitches, were included in the study. The bitches were divided into three groups a...

In dairy herds, cattle experience different health disorders, and their occurrence during lactation may cause important economic losses. The systematic analysis of productive and health records allows us to identify the risk factors of greatest negative incidence on profitability in commercial dairy farms. The aim of this work was to estimate the e...

In dairy herds, cattle experience different health disorders, and their occurrence during lactation may cause important economic losses. The systematic analysis of productive and health records allows us to identify the risk factors of greatest negative incidence on profitability in commercial dairy farms. The aim of this work was to estimate the e...

p>Los dispositivos intravaginales (DIV) con 300 mg de progesterona (P4) pueden reutilizarse en los protocolos de sincronización en ovinos con una duración de hasta 7 días. La reutilización de DIV con menor concentración hormonal, así como estudios far- maco-cinéticos a través del análisis no compartimental de las concentraciones hormonales luego de...

The main aim of this study was to assess the associations between the timing of lameness clinical case occurrence in lactation with productive and reproductive performances in grazing Holstein cows. A cohort study was carried out on a dataset with records from a commercial dairy herd (Buenos Aires, Argentina) for cows that calved and were dried off...

The present study aimed to determine the effect of cat seminal plasma and purified llama ovulation-inducing factor (β-NGF) on ovarian activity in queens. Queens (n=6) were used for all the treatments in a crossover design with an interval time between treatments of three interestrus intervals. Forty-eight hours after the detection of an estrus vagi...

The aim of the present study was to compare the endometrial gene expression of epidermal growth factor receptor (EGFR), nodal growth differentiation factor (NODAL), prostaglandin‐endoperoxide synthase 2 (PTGS2), oestrogen receptor 1 (ESR1) and progesterone receptor (PGR) in repeat breeder cows (RBC) and non‐RBC during diestrus. Endometrial samples...

The aim was to study number, volume, apoptosis of corpora lutea (CL), and serum P4 concentrations in early, middle, and late diestrus of dogs. Thirty-six bitches were ovari-hysterectomized (OVX): Early Diestrus (Group [G]1; OVX 20 days after end of estrus [DEH]); Mid-diestrus (GII; OVX between 21 and 40 days after DEH), and Late-diestrus (GIII; OVX...

Una línea de investigación activa en el INIRIA es el estudio de la teoría de programación fetal y en particular en el ganado bovino de los tambos de la provincia de Buenos Aires. Los datos que son analizados en estos estudios están compuestos de registros de producción de leche individuales realizados en entidades de control lechero oficial nuclead...

Objective To assess the efficacy of antibiotic usage for the treatment of puerperal metritis (PM) and its association with reproductive performance, a retrospective cohort study including a total of 9168 records of cows from a dairy farm in Argentina was run. Material and methods Cows having a PM3 (metricheck, scale 0–3) and treated with ceftiofur...

Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bi...

The objective of this study was to evaluate the association between progesterone concentration on days 4 and 9 of the estrous cycle and endometrial transcriptome at day 9 in lactating grazing dairy cows. Blood samples were obtained on days 0, 4, and 9 for progesterone measurement by chemiluminescence. Cows were assigned to one of the following grou...

The main objective of this study was to evaluate the risk factors for late embryonic loss (LEL) in supplemented grazing dairy cows. Additional objectives were to assess the incidence of LEL and its association with the reproductive performance of cows. A data set containing productive, reproductive, and health records of 13,551 lactations was used....

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components –- cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations -– and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen tom...

During the last years buffalo reproductive researchers have been tried to develop and apply fixed timed artificial insemination protocols to increase pregnancy rates to maximize improvement. The objective of this paper is to evaluate the pregnancy rates of different protocols of synchronization (Ovsynch and progesterone implants) and resynchronizat...

The main objective of this study was to evaluate the risk factors for late embryonic loss (LEL) in supplemented grazing dairy cows. Additional objectives were to assess the incidence of LEL and its association with the reproductive performance of cows. A data set containing productive, reproductive, and health records of 13,551 lactations was used....

The objective of the present study was to assess the relationship between individual cow milk yield and fertility, accounting for the contextual effect of the herd. A dataset including 657,968 lactations from 677 dairy herds located in Buenos Aires province, Argentina from 2001 to 2012 was used. The odds of pregnancy by 100 DIMwere assessed by a mu...

Different therapeutic strategies have been used with the objective of improve luteal function to reduce embryonic losses. The objective of this work was to study the effect of the administration of GnRH or hCG at Day 4 post fixed time artificial insemination (FTAI) on reproductive efficiency in Merino sheep during the breeding season in North Patag...

The main objective was to assess the associations of subclinical hypocalcemia (SCH), diagnosed at parturition (SCH-0) and 7 d in milk (SCH-7), with fertility in a herd of grazing dairy cows. Additional objectives were to characterize Ca concentration on 0 and 7 d in milk (DIM), assessing the risk factors for SCH-0 and SCH-7 and also the relationshi...

Se probó si la administración de dos inyecciones de PGF2α en las primeras 48 h posparto disminuye la incidencia de retención de las membranas fetales (RMF) y de patologías uterinas en vacas lecheras. Se utilizaron 800 vacas de diferente número de partos, las cuales se asignaron aleatoriamente a dos tratamientos; PGF2α (n= 400) recibieron una inyecc...

The objective of the present study was to assess the relationship between individual cow milk yield and fertility, accounting for the contextual effect of the herd. A data set including 657,968 lactations from 677 dairy herds in Argentina from 2001 to 2012 was used. The odds of pregnancy by 100 d in milk (DIM) were assessed by a multilevel logistic...

Contents The aim of the study was to identify beta‐haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty‐one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) inclu...

In order to determine its effect on incidence of retained fetal membranes (RFM) and postpartum uterine pathologies in dairy cows, the administration of two injections of PGF2α in the first 48 h after parturition was tested. In this study, 800 cows with different parities were randomly assigned to two treatment groups: PGF2α (n= 400), receiving two...

En el presente estudio se determinó la prevalencia de endometritis subclínica y su efecto en la tasa de gestación en vacas lecheras en manejo intensivo. Entre los días 35 y 45 posparto se seleccionaron 237 vacas sin patologías uterinas (vacas con útero involucionado y sin secreciones vaginales anormales); a todas ellas se les tomó una muestra citol...

We determined the effect of GnRH or hCG treatment on day 4 post-time artificial insemination (FTAI) on the formation of accessory corpora lutea (acc-CL) and on the concentration of serum progesterone (P4) in sheep. Multiparous adult Merino ewes (n = 36) were synchronized for estrus using double injection of PGF2α agonist (125 μg Cloprostenol) with...

Contents Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin ( PG ) synthesis enzymes (cyclooxygenase [ COX 2], PTGES ‐1 and PGFS ) in the endometrium of bitches wi...

The objectives of this study were to assess the association of a 4-point scale of vaginal discharge score (VDS) with time to pregnancy to define criteria for a practical case of purulent vaginal discharge (PVD) in dairy cows, to test the risk factors for PVD, and, finally, the effect of a dose of PGF2α on cure and reproductive performance. In exper...

The objective of the present study was to test the association between the presence of fluid in uterine lumen and the fertility of repeat breeder cows (RBC). Grazing Holstein cows that were clinically normal and were not pregnant after at least 3 consecutive inseminations were defined as RBC and included in the study (n = 358). A cut-point ≥2 mm of...

The objective of this study was to assess the effect of a selective antibiotic treatment strategy based on a quick bacteriological on-farm test (Petrifilm, 3M Corp., St. Paul, MN) compared with the conventional antibiotic treatment of all cows having clinical endometritis (CE) defined by the presence of purulent vaginal discharge on both clinical c...

Ultrasonography was used to study follicular dynamics from the beginning of estrus to ovulation in pubertal gilts and post-weaning sows. Ultrasound turned out to be a useful tool to determine patterns of growth of preovulatory follicles, to predict ovulation time, and to design protocols for fixed time insemination.

Background Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could s...

Progesterone (P4) is a requirement for pregnancy development. Previous reports observed a maximal value of serum P4 concentration on 21 days after the first mating after which it slowly declines throughout the rest of pregnancy. Ultrasound examination should be performed to ensure that pregnancy interruption is complete. Limited information is avai...

La utilización de dispositivos intravaginales (DIV) liberadores de progesterona (P4) para la sincronización de celos en ovinos tiene su origen en la década de los ‘80 bajo el nombre comercial de CIDR-S®, con una concentración de 370mg de P4 (InterAg, Hamilton, New Zealand)2, como alternativa a la utilización de esponjas intravaginales impregnadas c...

Reproductive efficiency in sheep can be affected by losses during pregnancy which is one of the factors affecting production parameters of this species. Among prenatal losses, embryonic mortality is considered as the most important, and its main cause would be an inadequate luteal function. Therefore, different hormonal therapies have been used in...

p>El objetivo del trabajo fue determinar el grado de acuerdo entre dos métodos de identificación bacteriana, marcha bacteriológica convencional y espectroscopía infrarroja transformada de Fourier (TF-IR), así como determinar si la base de datos construida a partir de bacterias aisladas de úteros de vaca lecheras de Europa podía ser utilizada para i...

The objectives of this study were to determine the onset of puberty in yearling Angus bulls on pasture and to compare the use of scrotal perimeter and spermiogram to determine the onset of puberty under commercial conditions. A total of 286 yearling has puberty (scrotal circumference 27.9 ±0.2 cm; sperm concentration 50 x 106/mL, 10% motile). For o...

Our objective was to evaluate histological and ultrastructural changes in canine epididymis (n = 22) stored at 4°C in two media [physiological solution (PS) or egg yolk tris (TEY)]. The hypothesis was that the epididymides stored in TEY autolysis delays phenomena at the cellular level. Four epididymal tails were fixed and processed immediately and...

The objectives of the present study were to test the agreement between routine methods of bacteriological testing and the Fourier transformed infrared spectroscopy (FTIR) method for uterine bacterial identification, and if a database built with uterine bacteria from European dairy cows can be used to identify bacteria from Argentinean dairy cows. P...

Contents The oestrus cycle in the domestic bitch, a monoestrous species, differs considerably from that of other veterinary domestic animals species. In the bitch the combined use of e CG and h CG is effective to induce oestrus predictably and safely (Stornelli et al., Theriogenology, 78, 2012 and 1056). Although several studies were done to descri...

Ultrasonography has been used to diagnose and monitor pregnancy. However, in the queen, most of ultrasonographic and endocrinological studies have been performed using small number of observations during limited periods of pregnancy. The aim of this study was to derive equations to predict the gestation age and parturition time using ultrasonograph...

Repeat breeder cows (RBC) are a frequent problem in dairy farms that produce important economic losses. These cows are described as clinically normal but they fail to conceive after at least three consecutive insemination. The objective of the present review is to describe causes and proposed treatments to repeat breeder cows during the last years....

The objectives of this study were to assess the risk factors for clinical endometritis (CE), and its effects on reproduction in Holstein cows (n = 303). Cows had their vaginal discharges (VD) checked for pus 21, 31 and 41 days postpartum. Plasma blood samples were analyzed for metabolites and metabolic hormones. Data were analyzed with PROC MIXED,...

The objectives of this study were to assess the agreement between endometrial cytology and uterine biopsy for the diagnosis of subclinical endometritis (SEND) in grazing dairy cows, the interobserver agreement of the biopsy's readings, and the bacterial population isolated from the uterus of cows having SEND. In experiment 1, lactating Holstein cow...

The objectives of this study were to evaluate the efficacy of (1) administering ceftiofur hydrochloride in dairy cows with calving-related disorders to prevent metritis and (2) a combination of GnRH and PGF2α for the treatment of clinical endometritis, under Argentinean dairy farming conditions. Cows at high risk (HRC) for metritis (dystocia, RFM >...

Treatment with cloprostenol, a prostaglandin synthetic analogue, was evaluated in five queens with open-cervix pyometra. Cloprostenol was administered (5 μg/kg body weight SC) on three consecutive days and amoxicillin (20 mg/kg body weight IM) on seven consecutive days. Transient post-injection reactions caused by cloprostenol administration includ...

The objectives of this study were to assess the effect of the stage of estrous cycle on the percentage of endometrial polymorphonuclear cells (PMN) obtained by cytobrush to determine cutoff values for the diagnosis of subclinical endometritis under pastoral conditions, to measure the prevalence of subclinical endometritis 21 to 62 d in milk (DIM),...

The objectives of this study were to assess the risk factors for metritis, its effects on milk yield and on reproductive performance, and the efficacy of ceftiofur therapy in Holstein dairy cows. Cows (n = 303) from a commercial dairy herd in Argentina were studied. Cows were scored for body condition, and blood samples were collected on d -14, 7,...

The objectives of this study were to evaluate the efficacy of (1) administering ceftiofur hydrochloride in dairy cows with calving-related disorders to prevent metritis and (2) a combination of GnRH and PGF 2α for the treatment of clinical endometritis, under Argentinean dairy farming conditions. Cows at high risk (HRC) for metritis (dystocia, RFM...

Contents The objective of this study was to evaluate the efficacy of cloprostenol ( CLO ) or aglepristone ( ALI ) for pregnancy termination in queens at 21–22 and 35–38 days of gestation. Two experiments ( EXP ) were carried out to accomplish this aim. Thirty‐seven 12‐ to 14‐month‐old mixed breed queens were used in a randomized design. At oestrus,...

Contents The aim of this study was to assess the histological and ultrastructural changes in cat epididymides (n = 22) stored at 4°C in two different media [saline solution ( SAL ) or tris‐egg yolk ( TEY )]. Our hypothesis was that epididymides stored in TEY would have delayed epithelial cell autolysis. Four epididymides were fixed and processed im...

Contents The aim of this study was to assess whether refractoriness to long photoperiod ( LP ) could be reversed by subjecting tomcats to a period of short days. Our hypothesis was that photoperiod changes can avoid refractoriness and restore sperm quality and production to that before refractoriness. Tomcats (n = 6) were housed in a conditioned ro...

The aim of this study was to assess epididymal sperm characteristics and serum testosterone concentration in cats under natural photoperiod. The hypothesis was that natural photoperiod induces seasonal changes in spermatozoal quality and serum testosterone concentration. Mixed breed tomcats (n = 43) that underwent bilateral orchiectomy at a municip...

The objectives of this study were to assess the clinical and metabolic risk factors for clinical endometritis, the likelihood for having a normal vaginal discharge during postpartum, and the effects of endometritis on milk yield, reproductive efficiency, and metabolic status in Holstein cows. The study was conducted in a commercial dairy herd (Cord...

Different methods may be used for pregnancy diagnosis of the feline. Ultrasonography allows early diagnosis of pregnancy and gives information about embryo-fetus development. The aim of the study was to assess the relationship between ultrasonographic measurements and gestational age. Ten mixed breed queens, aged between 24 and 36 month were used i...

Introducción Si bien existe un reporte previo sobre los cambios estacionales de la morfología, producción espermática y concentraciones hormonales del elefante marino (Mirounga leonina) en Australia, no tenemos conocimiento de que exista un reporte similar en Argentina. Objetivo Describir morfológicamente testículo y epidídimo de un macho adulto de...

The cytobrush (CB) had proved to be the best technique for obtaining uterine cytology for the diagnosis of subclinical endometritis (SE) in postpartum dairy cows. The diagnosis is based on the percentage of neutrophil cells (PPMN) in an endometrial cytology smear according to days postpartum. Several authors have described a physiological increase...