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Rocío Núñez Calonge

Rocío Núñez Calonge
International Reproduction Unit · Research

PhD

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96
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Publications

Publications (96)
Article
Study question How many frozen embryos are stored in Spain destined for donation to other couples, research, destruction, own use or abandoned? Summary answer In Spain, 5% of frozen embryos are destined for donation to other patients, 17% are fated for research, 17,7% for destruction and 12% are abandoned What is known already According to the la...
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Purpose There is a considerable literature supporting the role of lipids in fertility. However, little is known about their impact on male and female gametes. Our study aimed to investigate the relationships between lipids levels in serum, follicular fluid and seminal plasma with ovarian response and sperm concentration regardless of age and body m...
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RESUMEN Objetivo: El objetivo de este trabajo es analizar y comparar las fluctuaciones del índice de fragmenta-ción en dos poblaciones de varones: donantes de semen de fertilidad probada, y pacientes que acuden para realizar estudio de fertilidad. En ambos casos se determinan las variaciones intra e inter-individuo a lo largo del tiempo. Material y...
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The plasma glycoprotein afamin has been previously identified as an alternative carrier protein for vitamin E in extravascular fluids such as plasma and cerebrospinal, ovarian follicular, and seminal fluids. However, to date, no study has established a relationship between afamin levels and infertility in women or men. The purposes of our study wer...
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Purpose To determine the consequences of an altered sperm fluorescence in situ hybridization (FISH) result for ART outcomes and the indications for a sperm FISH analysis. Methods Data from 439 infertile men were collected. Bivariate analyses were performed to determine the association of men’s age, seminal alterations, and sperm FISH indication, w...
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Nuñez-Calonge R, Rancan L, Cortes S, et al. Apoptotic markers and antioxidant enzymes have altered expression in cumulus and granulosa cells of young women with poor response to ovarian stimulation. J Reprod Biol Endocrinol. 2019;3(1):1-6. Background: The objective of this study was to evaluate apoptosis and oxidative stress markers in Granulose (G...
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RESUMEN La influencia de la edad paterna avanzada en los resultados reproductivos tras la realización de ciclos de FIV/ICSI es un tema controvertido. Existen trabajos que demuestran la influencia negativa de la edad paterna avanzada en la tasa de gestación y un aumento de la tasa de abortos, mientras que otros estudios no encuentran diferencias sig...
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tIn this article several members of diverse scientific associations and reproduction experts from Spain have updated different genetic and immunological procedure recommendations in couples affected by reproductive dysfunction with the goal of providing a set of useful guidelines for the clinic. The laboratory test has been considered as highly rec...
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Resumen Varios miembros de diferentes asociaciones científicas y expertos de la reproducción han actualizado las recomendaciones de estudio genético e inmunológico en las parejas con disfunción en la reproducción con el fin de mejorar la asistencia sanitaria. El estudio se ha considerado altamente recomendable cuando la prueba diagnóstica es releva...
Article
In this article several members of diverse scientific associations and reproduction experts from Spain have updated different genetic and immunological procedure recommendations in couples affected by reproductive dysfunction with the goal of providing a set of useful guidelines for the clinic. The laboratory test has been considered as highly reco...
Article
Full-text available
There is a considerable literature supporting the role of lipids in male and female fertility, but little is known about their impact on male and female gametes. The aim of this study is to obtain, through principal component analysis (PCA), a lipid profile in plasma and common for men and women, and also specific lipid profiles in seminal plasma a...
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Disclosure: The authors have declared no conflicts of interest. Citation: EMJ Repro Health. 2017;3[1]:XX-XX. Abstract Review No. ARXX.
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La Reproducción Asistida ha experimentado progresos médicos y tecnológicos enormes e impensables. El número de tratamientos que se realizan en todo el mundo aumenta cada vez más, y son millones los niños nacidos por estos procedimientos. Desde el punto de vista médico, clásicamente, la solución a los problemas morales surgidos en el ejercicio profe...
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Introduction One of the additional tests for the diagnosis of male infertility is the study of sperm DNA fragmentation. One reason that may explain the damage of the DNA molecule is oxidative stress. This is caused by free oxygen radicals, among which is the superoxide anion (O2.−). Objective To measure the oxidative stress excess in semen samples...
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The aim of this study was to determine the concentrations of oxidative stress markers, antioxidant enzymes and cytokines in the follicular fluid of young women with low response in ovarian stimulation cycles compared with high responders and fertile oocyte donors of the same age, to assess the impact of oxidative stress on ovarian reserve. The acti...
Article
In most assisted reproduction laboratories, cultivation of human embryos is performed using atmospheric concentrations of oxygen (20 % O2), when the true physiological concentrations are lower. So far it is unclear whether these differences in the concentration of O2 represent a benefit for embryonic development or not. The benefit or harm can occu...
Conference Paper
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COGI Budapest 2015 - Abstract Submission Topic: ART/IVF COGIBudapest15-ABS-1118 mRNA EXPRESSION OF APOPTOTIC MARKERS AND ANTIOXIDANT ENZYMES IN CUMULUS AND GRANULOSE CELLS FROM PERIOVULATORY FOLLICLES OF YOUNG WOMEN WITH LOW OVARIAN RESERVE R. Nuñez-Calonge 1,*, S. Cortes 1, L. Rancan 2, E. Vara 2, L. Ortega 1, P. Caballero Peregrin 1, J. A. Fernán...
Article
Varicocele is a frequent cause of impaired testicular function that has been associated with increased levels of sperm DNA fragmentation (SDF). Sperm with degraded DNA (DDS), as observed using the sperm chromatin dispersion (SCD) test, represent a subpopulation of spermatozoa with extensive DNA and nuclear protein damage. The aim of this work was t...
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RESUMEN Existen evidencias crecientes en la literatura que demuestran que la estimulación ovárica, la cual produce niveles suprafisiológicos de hormonas, puede disminuir la tasa de gestación frente a los ciclos de crio-transferencias. Además, el desarrollo endometrial puede controlarse de forma más precisa en los ciclos con embriones congelados y d...
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Review of the social-demographic profile of gamete donors in a local sample in Spain: Motivation for donating, origin, and occupation Abstract The main objective of this study is to carry out a descriptive analysis of current data from men and women candidates to become gamete donors in Spain, in order to establish a social-demographic profile for...
Article
Growing evidence in the literature shows that controlled ovarian stimulation, with its supraphysiologic hormonal levels, may decrease pregnancy rate against frozen-thawed cycles. Moreover, endometrial development can be controlled more precisely during its priming for frozen-thawed embryo transfer vs. for controlled ovarian stimulation. Therefore,...
Conference Paper
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OXIDATIVE STRESS MARKERS IN FOLLICULAR FLUID OF YOUNG WOMEN WITH LOW RESPONSE IN OVARIAN STIMULATION DURING IN VITRO FERTILIZATION COMPARED TO FERTILE OOCYTE DONORS. R. NUÑEZ-CALONGE1, S.CORTES1, P.CABALLERO1, E.VARA2, R. KIREEV2, J.FERNANDEZ-TRESGUERRES2. 1 Unidad de Reproducción. Clínica Tambre; Madrid-Spain 2 Departamento de Fisiología, Facult...
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This study compared the potential of assessing sperm DNA fragmentation (SDF) from neat semen and the subsequent swim-up (SU) procedure to predict pregnancy when conducting ICSI of fertile donor oocytes. Infertile females (n=81) were transferred embryos resulting from intracytoplasmic sperm injection (ICSI) of their partner's spermatozoa and proven...
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Esta revisión está enfocada en la interacción entre las células del cúmulo y el espermatozoide. A pesar de la importancia de la penetración espermática en las células del cúmulo en el mecanismo de la fecundación, el proceso molecular sigue siendo controvertido. Además, el concepto general, que se ha mantenido durante mucho tiempo, se desprende sobr...
Conference Paper
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Luteal hormonal profile of the same oocyte donors stimulated with either GnRH antagonist or agonist compared with natural cycles. Study question The aim of the present study was to characterize hormonal profile during the early and the mid-secretory phase of unsupplemented in vitro fertilization cycles (IVF) in the same egg donors, stimulated in...
Article
Introduction: A certain level of damaged DNA is present in mature sperm cells after a natural ejaculation. Additionally, sperm management for artificial reproduction techniques (ART) may increase the basal rate of DNA damage. Unfortunately, the mechanisms and processes involved in Sperm DNA Fragmentation (SDF) are not fully illuminated. The main ai...
Conference Paper
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Conclusions Our results demonstrate that different levels of OS markers, oxidant enzymes and cytokines in low responder patients as compared to oocyte donors may have a negative impact on ovarian response. This is the first study analyzing the relationship between oxidative stress and low response in young patients undergoing controlled ovarian sti...
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13 Introducción En 1983, Trounson y Mohr 1 publicaron el pri-mer embarazo conseguido a partir de un em-brión humano criopreservado tras fecundación in vitro (FIV), y el primer nacimiento de un niño con embriones congelados tuvo lugar en 1984 en Australia 2 . Desde entonces, la criopreservación embriona-ria se ha convertido en una práctica cotidiana...
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Using donor oocytes of proven fertility, the effect of sperm DNA fragmentation (SDF) and motility on reproductive success was examined in 70 couples undergoing ICSI. Both SDF and sperm motility were assessed at the time of sperm injection and using the same sperm sample that was processed for ICSI. While there was no difference in the fertilization...
Article
The objective of this study was to identify the endometrial gene expression profile in receptive phase, which could represent a useful prognostic tool for selecting IVF patients. Endometrial expression of 47 selected genes biopsied during the window of implantation in natural cycles was compared between patients who achieved a successful pregnancy...
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Introduction Assisted Reproductive Technologies (ARTs) can be very helpful for certain patients, but ethical concerns have been raised about the inherent nature of specific techniques and the contexts in which many techniques are used. Actual and alleged unethical conduct in ART has generated public debate. All of this makes patients seeking ART pa...
Conference Paper
Background and Aim: Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. However, it has been noted that SDI can fluctuate over time within certain individuals. The aim of this study was to analyze and compare both within-and between-subject sperm DNA fragmentation (SDF) fluctuations in men presenting to an infertili...
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Objective. To optimise the use of freeze/thaw testicular immotile spermatozoa from nonobstructive azoospermia patients and to analyse the outcome of intracytoplasmic sperm injection (ICSI) of such spermatozoa. Methods. Testicular specimens were retrieved and cryopreserved from forty patients with nonobstructive azoospermia and underwent one cycle w...
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IntroductionHigh levels of oxidative stress can explain the presence of high levels of damage in the DNA molecule. The impact of high levels of oxidative stress in 2 clinical circumstances affecting the male germ line has been well established: leukocytospermia and varicocele.Objective To assess sperm DNA fragmentation in patients diagnosed with le...
Article
Introduction: High levels of oxidative stress can explain the presence of high levels of damage in the DNA molecule. The impact of high levels of oxidative stress in 2 clinical circumstances affecting the male germ line has been well established: leukocytospermia and varicocele. Objective: To assess sperm DNA fragmentation in patients diagnosed wit...
Article
Introduction To insure reproducibility of all methods used in a Reproduction Centre and competence in all duties performed by the staff it is of primordial importance to implement a quality control system. Although much focus is placed upon pregnancy rates, the use of pregnancy rates as the most important criteria for the measure of quality in repr...
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The rate of increase of sperm DNA fragmentation (rsDF) in fresh and frozen-thawed and processed sperm samples after a density gradient for sperm selection was analysed after 0, 0.5, 1.5, 4.5, 6, 24, 48 and 72 h of incubation at 37 °C, in five donors with proven fertility. The results showed that: (i) sperm DNA fragmentation (sDF) at baseline in fre...
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For abstracts see the DOI resource. https://doi.org/10.1093/humrep/26.s1.75
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Introduction While sperm DNA Fragmentation (SDF) is a significant factor in the prognosis of male fertility, it has been noted that baseline levels of SDF sometimes vary when repeated ejaculates of the same individual are compared. The aim of this study was to analyze and compare both within and between individual baseline SDF fluctuations in two d...
Article
IntroductionSperm DNA integrity is one of the most important factors currently analyzed in the study of male fertility. There are numerous publications on the utility of diverse methods analyzing sperm chromatin status. One of the simplest is the ethidium bromide/acridine orange (AO) test, which assesses the degree of sperm DNA denaturation accordi...
Article
Introduction: Sperm DNA integrity is one of the most important factors currently analyzed in the study of male fertility. There are numerous publications on the utility of diverse methods analyzing sperm chromatin status. One of the simplest is the ethidium bromide/acridine orange (AO) test, which assesses the degree of sperm DNA denaturation accor...
Article
Equilibrium thawing was performed using Cook Freezing and thawing kits. During this period, there were 97 warming cycles with vitrified embryos and 88 thaw cycles with embryos frozen using the equilibrium method. Data was compared from the two groups using survival rate and pregnancy rate as the primary outcome measures. Results: We compared 88 emb...
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Introduction Flow cytometry (FCM) has developed into a highly reliable technology that can serve the andrology laboratory and infertility practices. New fluorescent stains and techniques that have potential application to the FCM evaluation of sperm DNAchromatin integrity have continuously being developed taking into consideration the pivotal role...
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Single- and double-strand sperm DNA breaks was assessed in a Kartagener's syndrome with four failures of fertilization after ISCI in TESA samples. It is concluded that in addition to failure of sperm motility, this patient was infertile because a high level of non-reparable sperm DNA damage was present. Although four cycles of insemination were per...
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The aim of this study was to compare the rate of sperm DNA fragmentation (rSDF; increase of SDF over time) in fresh and gradient isolated frozen-thawed semen samples from male sperm donors of proven fertility. SDF was assessed in the two samples obtained from the same fifteen male donors after 0.5, 1.5, 4.5, 6, 24, 48, and 72 h of incubation in a h...
Article
The immotile cilia syndrome is an autosomal recessive disease characterized by defective cilial ultraustructure in ciliated cells. Men with simultaneous presence of situs inversus and bronchiectasis are referred to as having Kartagener's syndrome. The objective of this report is to describe a case of a male with Kartagener's syndrome with four fail...
Article
The immotile cilia syndrome is an autosomal recessive disease characterized by defective cilial ultraustructure in ciliated cells. Men with simultaneous presence of situs inversus and bronchiectasis are referred to as having Kartagener's syndrome. The objective of this report is to describe a case of a male with Kartagener's syndrome with four fail...
Conference Paper
Full-text available
The aim of this study was to optimise the use of freeze/thaw testicular immotile spermatozoa from nonobstructive azoospermia patients. Testicular specimens were retrieved and cryopreserved from twenty-five patients with nonobstructive azoospermia and underwent one cycle with thawed spermatozoa (Group I) that led to pregnancy in ten cases. Fifteen p...
Article
To analyze any possible dynamic correlation between sperm DNA fragmentation and sperm viability. The rate of viability loss and the rate of increase of the frequency of sperm cells with fragmented DNA were determined at 0, 1.5, 4.5, and 24.0 hours after thawing samples from donors with proven fertility. Academic biology and reproductive medicine ce...
Article
To study the velocity of sperm DNA fragmentation in frozen-thawed sperm samples from male sperm donors of proved fertility. Sperm DNA fragmentation assessment with use of sperm chromatin dispersion methodology after 0, 4, 8, and 24 hours of incubation in IVF medium. Academic biology and reproductive medicine centers. Twenty male fertility donors wi...
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IntroductionFluorescent in situ hybridization (FISH) in decondensed sperm nuclei has proven to be a good method for determining the chromosome content of spermatozoa. Even among infertile men with a normal karyotype, there is a considerable frequency of chromosomal abnormalities limited to the germ line.In the present study, we have retrospectively...
Article
IntroductionThe dynamics of sperm DNA fragmentation (SDF) using the semen samples before and after capacitation have been investigated in a prospective study. All samples were processed with Halosperm for the SDF analysis.
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Sperm DNA fragmentation and its relationship to fertility is a topic of great interest, not only in humans but in all species, given the implicit role of the DNA molecule in producing a successful offspring with a genetic resemblances to the parental genomes. However, sperm DNA fragmentation is not easy, mainly because the evolutionary trends have...
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Objectives: The aim of this study was to demonstrate that the sperm DNA in humans and other mammals, when manipulated for assisted reproduction techniques, is stressed and causes a progre s s ive increase in the basal level of DNA fragmentation. Materials and methods: 10 human donors with proven fertility and 10 stallions, 10 rams, and 10 bulls wer...