Roberto Pinton

Roberto Pinton
University of Udine | UNIUD · Department of Agricultural, Food, Environmental and Animal Sciences

PhD

About

156
Publications
30,878
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4,765
Citations
Additional affiliations
October 1986 - present
University of Udine
Position
  • Professor (Full)
Description
  • Research activity is focussed on mechanisms of plant nutrition, with special regard to iron and nitrogen, and on soil-plant relationships with emphasis to the rhizosphere processes, root exudates release and the role of natural organic matter fractions.
Education
January 1983 - December 1985
University of Padova
Field of study
  • Crop physiology and productivity
October 1975 - March 1981
University of Padova
Field of study
  • Agricultural science

Publications

Publications (156)
Article
Full-text available
The wide use of copper (Cu)-based fungicide has caused a stepwise accumulation of Cu in the environment increasing the occurrence of phytotoxicity in crops. To understand and alleviate this abiotic stress, maize seedlings were grown in hydroponic solution with different combinations of Cu and iron (Fe) forms. Results showed that maize Cu sensitivit...
Article
Full-text available
The low bioavailability of nutrients, especially nitrogen (N) and phosphorus (P), is one of the most limiting factors for crop production. In this study, under N- and P-free nutrient solution (-N-P), nodulating white lupin plants developed some nodules and analogous cluster root structures characterized by different morphological, physiological, an...
Article
Full-text available
Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobi...
Preprint
Full-text available
Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by e.g., forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobial symbi...
Article
Full-text available
The simultaneous presence of different N-forms in the rhizosphere leads to beneficial effects on nitrogen (N) nutrition in plants. Although widely used as fertilizers, the occurrence of cross connection between urea and ammonium nutrition has been scarcely studied in plants. Maize fed with a mixture of urea and ammonium displayed a better N-uptake...
Article
Full-text available
To date urea and ammonium are two nitrogen (N) forms widely used in agriculture. Due to a low production cost, urea is the N form most applied in agriculture. However, its stability in the soil depends on the activity of microbial urease, an enzyme that operates urea hydrolyses into ammonium. In the soil ammonium is subjected to fast volatilization...
Article
Despite the wide use of urea and ammonium as N-fertilizers, no information is available about the proper ratio useful to maximize the efficiency of their acquisition by crops. Ionomic analyses of maize seedlings fed with five different mixes of urea and ammonium indicated that after 7 days of treatment, the elemental composition of plant tissues wa...
Article
Full-text available
Conversion of conventional farming (CF) to organic farming (OF) is claimed to allow a sustainable management of soil resources, but information on changes induced on dissolved organic matter (DOM) are scarce. Among DOM components, dissolved humic substances (DHS) were shown to possess stimulatory effects on plant growth. DHS were isolated from CF a...
Article
Full-text available
Nitrogen (N) deficiency is one of the major stresses that crops are exposed to. It is plausible to suppose that a stress condition can induce a memory in plants that might prime the following generations. Here, an experimental setup that considered four successive generations of N-sufficient and N-limited Arabidopsis was used to evaluate the existe...
Article
Full-text available
Iron and phosphorus are abundant elements in soils but poorly available for plant nutrition. The availability of these two nutrients represents a major constraint for fruit tree cultivation such as apple (Malus × domestica) leading very often to a decrease of fruit productivity and quality worsening. Aim of this study was to characterize common and...
Article
Full-text available
Improvement of plant iron nutrition as a consequence of metal complexation by humic substances (HS) extracted from different sources has been widely reported. The presence of humified fractions of the organic matter in soil sediments and solutions would contribute, depending on the solubility and the molecular size of HS, to build up a reservoir of...
Article
In agricultural soil, the bioavailability of iron (Fe) and phosphorus (P) is often below the plant's requirement causing nutritional deficiency in crops. Under P-limiting conditions, white lupin (Lupinus albus L.) activates mechanisms that promote P solubility in the soil through morphological, physiological and molecular adaptations. Similar chang...
Article
Full-text available
This DIB article provides details about transcriptional and physiological response of Fe- and P-deficient white lupin roots, an extensive and complete description of plant response is shown in the research article "Physiological and transcriptomic data highlight common features between iron and phosphorus acquisition mechanisms in white lupin roots...
Article
Full-text available
Aim The rate of nitrate (NO3⁻) uptake and changes in rhizosphere properties were studied growing seedlings of two maize inbred lines differing in nitrogen use efficiency (NUE) in rhizoboxes. Results Changes in NO3⁻ uptake rates occurred in response to anion addition (induction) in seedlings grown both in hydroponic culture and in soil in rhizoboxe...
Article
Humic substances are known to positively influence plant growth and nutrition. In particular, the water-extractable fraction of humic substances (WEHS) has been shown to enhance nitrate acquisition, increasing the activity of high affinity nitrate uptake system. However, molecular bases of this physiological response are not clarified so far. Thus,...
Article
Full-text available
Background Under limited iron (Fe) availability maize, a Strategy II plant, improves Fe acquisition through the release of phytosiderophores (PS) into the rhizosphere and the subsequent uptake of Fe-PS complexes into root cells. Occurrence of Strategy-I-like components and interactions with phosphorous (P) nutrition has been hypothesized based on m...
Article
Full-text available
In this study, we provide experimental evidences that in calcareous soils microbial degradation/decomposition of citrate can promote Al-(hydr)oxide precipitation concurrently decreasing copper (Cu) solubility by a coprecipitation process. Citrate is an organic acid anion commonly released by roots to increase nutrient availability or to limit Al to...
Article
Full-text available
To limit nitrogen (N) losses from the soil, it has been suggested to provide urea to crops in conjunction with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). However, recent studies reported that NBPT affects urea uptake and urease activity in plants. To shed light on these latter aspects, the effects of NBPT were studied analysin...
Data
MapMan Mapping File. Transcript mapping file for the pathway of urea assimilation and primary metabolism.
Data
MapMan Mapping File. Transcript mapping file for the pathway of phenylalanine/tyrosine derivatives.
Data
MapMan Pathway file. Image file for the pathway of urea assimilation and primary metabolism without transcript bins.
Data
Overview of up- (red) and down- (green) modulated transcripts in Urea+NBPT vs. Urea using MapMan-bincode classification. Numbers refer to the MapMan bincode: 1, PS; 2, major CHO metabolism; 3, minor CHO metabolism; 4, glycolysis; 5, fermentation; 6, gluconeogenesis/glyoxylate cycle; 7, OPP; 8, TCA/org transformation; 9, mitochondrial electron trans...
Data
Real-time RT-PCR validation of a set of genes differentially expressed in microarray analysis Urea+NBPT vs. Urea. In the table are shown (starting from left column): Number of transcript according to Tables 2–4, Supplementary Table 4, transcript ID, description, gene symbol, microarray value (fold change and p. value adj.), real-time RT-PCR values...
Data
Differentially expressed transcripts resulted by the comparison of root transcriptional profiles of Urea+NBPT plants with root transcriptional profile of Urea plants (Urea+NBPT vs. Urea comparison). In the table are shown (starting from left column): transcript ID, fold change (FC) of Urea+NBPT vs. Urea, adjusted P-value of Urea+NBPT vs. Urea, gene...
Data
MapMan Pathway file. Image file for the pathway of phenylalanine/tyrosine derivatives without transcript bins, image adapted from Tohge et al. (2013).
Article
Full-text available
Background: It is well known that in the rhizosphere soluble Fe sources available for plants are mainly represented by a mixture of complexes between the micronutrient and organic ligands such as carboxylates and phytosiderophores (PS) released by roots, as well as fractions of humified organic matter. The use by roots of these three natural Fe sou...
Article
Full-text available
Despite the widespread use of urease inhibitors in agriculture, little information is available on their effect on nitrogen (N) uptake and assimilation. Aim of this work was to study, at physiological and transcriptional level, the effects of N-(n-butyl) thiophosphoric triamide (NBPT) on urea nutrition in hydroponically grown maize plants. Presence...
Article
While nitrate acquisition has been extensively studied, less information is available on transport systems of urea. Furthermore, the reciprocal influence of the two sources has not been clarified, so far. In this review, we will discuss recent developments on plant response to urea and nitrate nutrition. Experimental evidence suggests that, when ur...
Article
Soilless cultivation systems represent an alternative to traditional agriculture, as they offer the possibility to reduce water use and to design nutrient formulations of the hydroponic solutions in order to maximize yield and quality of the products. Reduction in accumulation of undesired compounds, like e.g. nitrate, and enhanced nutritional valu...
Article
Combinations of different molecules usually co-exuded by plant roots in soil can significantly affect the mobilization of mineral elements from soil. The flavonoids rutin and quercetin appeared to be highly efficient in Fe and Mn mobilization from soil, being rutin about 25 times more effective than citrate in extracting Fe from an alkaline calcare...
Article
Background and AimsNitrate is a major form of inorganic nitrogen present in cultivated soils; however, information on the mechanisms responsible for uptake of the anion in grapevines is scarce.Methods and ResultsThe response to external nitrate was studied in two clones of the cultivars Chardonnay and Sauvignon Blanc. Grapevines, own-rooted or graf...
Article
There is increasing interest in the hydroponic technology to produce leafy vegetables for ready-to-eat salads. Optimisation of the growing system can lead to higher yield and/or improved nutritional value of the product. Selenium (Se) is an essential element for animal and humans, with quite a narrow range between deficiency and toxicity, whereas i...
Article
Even if urea and nitrate are the two major nitrogen (N) forms applied as fertilizers in agriculture and occur concomitantly in soils, the reciprocal influence of these two N sources on the mechanisms of their acquisition are poorly understood. Therefore, molecular and physiological aspects of urea and nitrate uptake were investigated in maize (Zea...
Article
Full-text available
Water temperature plays a relevant role in the growth and development of plants in floating system cultivation, thus affecting both productivity and quality. A model of a greenhouse which hosts ponds is described, in order to highlight the components of the energy balance and estimate the refrigerating power required to maintain the nutrient soluti...
Article
Full-text available
Background Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in few plant species highlighting the importance of a high-affinity transport system. Wit...
Article
Plants mainly rely on a mixture of Fe complexes with different organic ligands, like carboxylates and soluble fractions of water-extractable humic substances (WEHSs), to sustain the supply of this micronutrient. It has been demonstrated that the Fe-WEHS complex is more efficiently acquired by plant roots as it enhances functionality of the mechanis...
Article
Full-text available
Poor iron (Fe) availability in soil represents one of the most important limiting factors of agricultural production and is closely linked to physical, chemical and biological processes within the rhizosphere as a result of soil–microorganism–plant interactions. Iron shortage induces several mechanisms in soil organisms, resulting in an enhanced re...
Article
Full-text available
This study addresses the question of the interference between Fe nutrition and Cd toxicity at the level of growth performance, phytosiderophores (PS) release, micronutrient accumulation and expression of genes involved in Fe homeostasis in barley seedlings, a plant with Strategy II-based response to Fe shortage. Cd exposure induced responses simila...
Article
Plants differ in their response to high aluminium (Al) concentrations which typically cause toxicity in plants grown on acidic soils. The response depends on plant species and environmental conditions such as substrate and cultivation system. The present study aimed to assess Al-phosphate (P) dynamics in the rhizosphere of two bean species, Phaseol...
Article
Full-text available
The rationale of this paper is to review the state of the art regarding the biotic and abiotic reactions that can influence Fe availability in soils. In soil, the management-induced change from oxic to anoxic environment results in temporal and spatial variations of redox reactions, which, in turn, affect the Fe dynamics and Fe mineral constituents...
Article
Full-text available
The development of highly brilliant synchrotron facilities all around the world is opening the way to new research in biological sciences including speciation studies of trace elements in plants. In this paper, for the first time, iron (Fe) speciation in xylem sap has been assessed by X-ray absorption near-edge structure (XANES) spectroscopy at the...
Article
Full-text available
Iron (Fe) is an essential element for plant growth and development; hence determining Fe distribution and concentration inside plant organs at the microscopic level is of great relevance to better understand its metabolism and bioavailability through the food chain. Among the available microanalytical techniques, synchrotron μ-XRF methods can provi...
Article
Cadmium (Cd) detoxification involves glutathione and phytochelatins biosynthesis: the higher need of nitrogen should require increased nitrate (NO(3) (-)) uptake and metabolism. We investigated inducible high-affinity NO(3) (-) uptake across the plasma membrane (PM) in maize seedlings roots upon short exposure (10 min to 24 h) to low Cd concentrati...
Article
Humic fractions were shown to be closely involved in gene expression and promotion of different PM H+-ATPase isoforms, as well as in lateral root development, indicating an enhanced nutrient absorption capacity of the plant root system. HPLC-SEC confirmed that water-soluble humic substances (WSHS) correspond to a subfraction of the fulvic fraction...
Article
Full-text available
In the present study, five insoluble Fe-humic substance (HS) complexes (Fe-IHS) extracted from Leonardite were synthesized and characterized by Infrared (IR) spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The efficiency of Fe-IHS complexes in curing Fe chlorosis was compared with two soluble Fe sources (Fe com...
Article
Full-text available
The mechanisms by which nitrate is transported into the roots have been characterized both at physiological and molecular levels. It has been demonstrated that nitrate is taken up in an energy-dependent way by a four-component uptake machinery involving high- and low- affinity transport systems. In contrast very little is known about the physiology...
Article
Soil-less cultivation of horticultural crops represents a fairly recent innovation to traditional agriculture which has several advantages including higher water-use efficiency. When plants are grown with this system, their roots come in contact with nutrients solely via the hydroponic solution. Although its beneficial effects have been widely demo...
Article
Plants release significant amounts of high and low molecular weight organic compounds into the rhizosphere. Among these exudates organic acids (e.g. citric acid, malic acid, oxalic acid), phenolic compounds (e.g. flavonoids), amino acids and siderophores of microbial and/or plant origin strongly influence and modify the biogeochemical cycles of sev...
Article
Full-text available
Plants react to iron deficiency stress adopting different kind of adaptive responses. Tomato, a Strategy I plant, improves iron uptake through acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells. Large-scale transcriptional analyses of roots under iron deficiency are only available for a very limited number...
Data
Primer sequences of Real-time RT-PCR experiment. TC ID, description and sequences of forward and reverse primers are reported.
Data
Functional annotation of 97 differentially expressed transcripts. ProbeID. Fold change. adjusted p-value. reference Tentative Consensus sequence in DFCI Tomato Gene Index (Release 12.0) http://compbio.dfci.harvard.edu/cgi-bin/tgi/gimain.pl?gudb=tomato. description. UniProtID http://www.uniprot.org/. Biological process GO term and E-value are report...
Article
Full-text available
Plants produce and release in the surrounding soil, the so-called rhizosphere, a vast variety of secondary metabolites. Among them, flavonoids are the most studied, mainly for their role in the establishment of rhizobium-legume symbiosis; on the other hand, some studies highlight that they are also important in the plant strategies to acquire nutri...
Article
The aim of this work is to evaluate the capability of tomato plants to use different Fe sources, such as Fe citrate, Fe phytosiderophores, and Fe complexed by a water-extractable humic substances (Fe-WEHS) also in relation to physiological and molecular adaptations induced by these complexes at the root level. Tomato plants acquired higher amounts...
Article
Full-text available
Soil temperature has a crucial impact on physiological processes and growth of plants with important consequences for plant productivity and food safety including nitrate accumulation in leaf blades of leaf vegetables. Consumer demand for high-quality, fresh-cut vegetables has increased rapidly in the last decades, and temperaturemodulation can hel...
Article
Full-text available
Both Fe deficiency and Cd exposure induce rapid changes in the S nutritional requirement of plants. The aim of this work was to characterize the strategies adopted by plants to cope with both Fe deficiency (release of phytosiderophores) and Cd contamination [production of glutathione (GSH) and phytochelatins] when grown under conditions of limited...
Article
Full-text available
The estimate of world population growth and the extent of malnutrition problems due to lack of food or to deficit of specific micronutrients bring to light the importance of plant nutrition in the context of a sustainable development. Beside these aspects, which force to use fertilizers, the topic of nutrient use efficiency of by plants is far from...
Article
Full-text available
Unbalanced nutrient availability causes disequilibrated plant growth, which can result in a worsening of harvested product quality, such as high nitrate content in edible tissues. To cope with this problem, improved knowledge of the mechanisms involved in nutrient acquisition and regulation is necessary. For this purpose the responses of acquisitio...
Article
Main components of Strategy II mechanism for Fe uptake are secretion of chelating compounds, phytosiderophores, and specific uptake of Fe(III)-phytosiderophores complex. Since the amount of phytosiderophores secreted correlates positively with plant ability to cope with Fe shortage, a role of root hairs in enhancing root capability to store phytosi...
Article
Plants release a multitude of organic compounds into the rhizosphere, some of which are flavonoids. These products of secondary metabolism are mainly studied for their antioxidant properties and for their role in the establishment of rhizobium-legume symbiosis; however, it has been recently demonstrated that flavonoids can also affect nutrient avai...