Roberto Lazzarini

• Doctor of Philosophy
• Laboratorio Divisional de Microscopia Confocal at Metropolitan Autonomous University

En la embriogénesis, el corazón es el primer órgano que se forma y adquiere capacidad funcional. El desarrollo cardíaco en humanos sucede de la segunda a la octava semana de gestación y se considera un proceso dinámico y complejo, que involucra la coordinación espaciotemporal de diferentes poblaciones celulares, mediadas por la activación y supresi...

RESUMEN. En la actualidad, se considera que la tabicación cardíaca es resultado de una serie de eventos moleculares, celulares y tisulares que convergen espaciotemporalmente para formar un corazón con cuatro cámaras, donde la circulación arterial se mantiene separada de la circulación pulmonar por la presencia de tres tabiques cardíacos: el tabique...

Resumen. La historia evolutiva del corazón comienza con la aparición de la célula eucariota y los primeros animales triblásticos en el subreino Bilateria quienes poseían un tubo pulsátil sin cámaras ni válvulas. A lo largo de la evolución, el corazón experimentó innumerables cambios. Los vertebrados más primitivos muestran un corazón tubular, los p...

Transcription regulation in cestodes has been little studied. Here, we characterize the Taenia solium TATA-binding protein (TBP) gene. We found binding sites for transcription factors such as NF1, YY1, and AP-1 in the proximal promoter. We also identified two TATA-like elements in the promoter; however, neither could bind TBP. Additionally, we mapp...

This work describes a first attempt of palindromic design for dual compounds that act simultaneously on peroxisome proliferator‐activated receptor gamma (PPARγ) and G‐protein‐coupled receptor 40 (GPR40) for the treatment of type 2 diabetes. The compounds were synthesized by multi‐step chemical reactions and the relative mRNA expression levels of PP...

Background/objetives: Mexican people use Smilax dominguensis as a traditional medicine for diabetes control. Some reports have shown an anti-hyperglycemic effect in animal models. In the current research, a chemical bio-guided fractionation in vitro and in silico was performed to identify compounds with anti-hyperglycemic and hypolipidemic effects...

El corazón depende de señales eléctricas para su función contráctil, las cuales se propagan entre los cardiomiocitos a través de uniones comunicantes formadas por conexinas, especialmente la conexina 43 (Cx43). Esta proteína, predominante en el miocardio ventricular y localizada en los discos intercalares, facilita el paso de iones y pequeñas moléc...

RESUMEN. La tabicación cardiaca es resultado de una serie de eventos moleculares, celulares y tisulares que convergen espaciotemporalmente para formar un corazón con cuatro cámaras, donde la circulación arterial se mantiene separada de la circulación pulmonar por la presencia de tres tabiques cardíacos: el tabique interatrial, el tabique interventr...

El desarrollo embrionario es un proceso complejo y dinámico, el cual ha llamado la atención de la humanidad desde hace miles de años. A lo largo de la historia, filósofos, naturistas y científicos pioneros se dedicaron a cuestionar la formación de la vida. Curiosamente, las respuestas a muchos de sus cuestionamientos las encontraron al abrir un hue...

Development and formation of the heart, the central organ of the circulatory system in vertebrates, starts early during embryonic development (second week), reaching maturity during the first few postnatal months. Cardiogenesis is a highly complex process that requires the active and orderly participation of different cardiac and non-cardiac cell p...

Hyperglycemia during gestation can disrupt fetal heart development and increase postnatal cardiovascular disease risk. It is therefore imperative to identify early biomarkers of hyperglycemia during gestation-induced fetal heart damage and elucidate the underlying molecular pathomechanisms. Clinical investigations of diabetic adults with heart dysf...

Alcohol-induced pancreas damage remains as one of the main risk factors for pancreatitis development. This disorder is poorly understood, particularly the effect of acetaldehyde, the primary alcohol metabolite, in the endocrine pancreas. Hepatocyte growth factor (HGF) is a protective protein in many tissues, displaying antioxidant, anti-apoptotic,...

Abstract Aim: Cholestasis remains a partially characterized disease. Evidence has been gained that it is a systemic disease that begins in the liver but significantly impacts other organs and systems such as the kidney, heart, and intestine, among others. One of the primary damage mechanisms is the generation of reactive oxygen species (ROS), which...

Hepatocyte growth factor (HGF) has been proved to protect the liver against α-naphthylisothiocyanate (ANIT)-induced cholestasis by acting as an antioxidant agent and redirecting toxic biliary solutes towards blood for urinary excretion. However, this may represent an additional potential risk for kidney integrity, which is already compromised by th...

The loss of skeletal muscle mass and strength is known as sarcopenia; it is characterized as a progressive and generalized muscle disorder associated with aging. This deterioration can seriously compromise the elderly's health and reduce their quality of life. In addition to age, there are other factors that induce muscle mass loss, among which are...

Recent discoveries of at least two heart fields and dynamic nature of cardiac development as well as controversies regarding the participation of heart fields in development of different heart structures led us to investigate the dynamics of incorporation of the first and second heart fields and prospective fate of the straight heart tube by labeli...

Cholestasis is a clinical syndrome common to a large number of hepatopathies, in which either bile production or its transit through the biliary tract is impaired due to functional or obstructive causes; the consequent intracellular retention of toxic biliary constituents generates parenchyma damage, largely via oxidative stress-mediated mechanisms...

In Central and South American traditional medicine, people use Cecropia obtusifolia Bertol (Cecropiaceae) for the treatment of diabetes mellitus. However, its hypoglycemic action mechanism at pancreatic and liver level has been poorly explored. The present research aimed to establish the influence of the aqueous extract of C. obtusifolia, standardi...

Sarcopenia is a syndrome characterized by a progressive and generalized skeletal muscle mass and strength loss, as well as a poor physical performance, which as strongly been associated with aging. Sedentary lifestyle in the elderly contributes to this condition; however, physical activity improves health, reducing morbidity and mortality. Recent s...

Growth differentiation factor 11 (GDF11) has been characterized as a key regulator of differentiation in cells that retain stemness features, despite some controversies in age-related studies. GDF11 has been poorly investigated in cancer, particularly in those with stemness capacity, such as hepatocellular carcinoma (HCC), one of the most aggressiv...

Objective There is no consensus on the embryonic components or morphogenetic processes involved in mature ventricular outflow tract development. Our goal was to use in vivo labelling to investigate the prospective fate of the myocardium of each conal wall. The conal and atrioventricular cushion mesenchyme changes during transformation into mature s...

Glycine modulates inflammatory processes mediated by macrophages and adipocytes through decreasing the secretion of TNF-α, IL-6, and leptin, while increasing adiponectin. These effects have been associated with the inactivation of NF-κB in response to TNF-α, across an increase of its inhibitor IκB-α in adipocytes. However, glycine upstream mainly i...

Acute pancreatitis is a multifactorial disease associated with profound changes of the pancreas induced by release of digestive enzymes that lead to increase in proinflammatory cytokine production, excessive tissue necrosis, edema, and bleeding. Elevated levels of hepatocyte growth factor (HGF) and its receptor c-Met have been observed in different...

Las bebidas energizantes son productos que prometen mediante su consumo, aliviar la fatiga, mantener la vigilia, mejorar el rendimiento físico y estimular las capacidades cognitivas ante situaciones de estrés. Se componen básicamente de cafeína, aminoácidos, vitaminas, minerales, extractos vegetales, aditivos acidulantes, saborizantes y colorantes....

Background and aim. Growth differentiation factor 11 (GDF11) has been recently characterized as an important regulator of proliferation and differentiation in cells that retain stemness features but its role in cancer cells is not characterized yet. Here, we focused to elucidate the regulatory effects induced by GDF11 in hepatocellular carcinoma (H...

The endocannabinoid system (ECS), and agonists acting on cannabinoid receptors (CBr), are known to regulate several physiological events in the brain, including modulatory actions on excitatory events probably through N-methyl-D-aspartate receptor (NMDAr) activity. Actually, CBr agonists can be neuroprotective. The synthetic CBr agonist WIN55,212-2...

El cáncer de mama se posiciona como la neoplasia más común entre las mujeres a nivel mundial, haciendo importante la búsqueda de nuevos agentes que actúen contra esta enfermedad. La nanotecnología propone el uso de nanomateriales principalmente elaborados con oro (nanoAu). Ensayos in vitro sobre células tumorales indican que las nanoAu inducen su c...

TATA-box binding protein (TBP) is an essential regulatory transcription factor for the TATA-box and TATA-box-less gene promoters. We report the cloning and characterization of a full-length cDNA that encodes a Taenia solium TATA-box binding protein 1 (TsTBP1). Deduced amino acid composition from its nucleotide sequence revealed that encodes a prote...

Cancer chemotherapy remains one of the preferred therapeutic modalities against malignancies despite its damaging side effects. An expected outcome while utilizing chemotherapy is apoptosis induction. This is mainly regulated by a group of proteins known as the Bcl-2 family, usually found within the endoplasmic reticulum or the mitochondria. Recent...

La venta libre de productos cosméticos con nanopartículas de oro se ha incrementa-do considerablemente en los últimos años. Adicionalmente, las nanopartículas de oro se posicionan como opciones terapéuticas principalmente contra el cáncer. Sin embargo, no se conoce a fondo los posibles efectos tóxicos de estas nanopartículas en células ni organismo...

The effects petroleum hydrocarbons (PH) on the morphological and physiological responses of roots have not been well studied. This research evaluated the root hydraulic responses of Medicago sativa seedlings exposed to diesel. Seeds were germinated in test tubes with 1% water-agar contaminated with diesel (3 000, 6 000, 9 000, 12 000, 15 000, 18 00...

En el desarrollo embrionario, los procesos del desarrollo son modulados a través de la comunicación celular. Unas células sintetizan y secretan moléculas denominadas ligandos y otras células reciben estas señales por medio de receptores a los que se unen específicamente los ligandos. La unión del ligando al receptor inicia la transducción de la señ...

Tumor cells utilize inappropriate epithelial-mesenchymal transition (EMT) mechanisms during the invasive process. It is becoming increasingly clear that estradiol (E2) induces breast cancer cell progression and enhances EMT; however, the mechanisms associated with this are unclear. We investigated the role of E2 on the expression and intracellular...

Astrocytes are key players for brain physiology, protecting neurons by releasing antioxidant enzymes; however, they are also susceptible to damage by neurotoxins. Nuclear factor erythroid-derived 2-like 2 (Nrf2) is a central regulator of the antioxidant response, and therefore, pharmacologic inducers are often used to activate this transcription fa...