Robert Miller

Robert Miller
University of Brasília | UnB · Department of Cell Biology

About

177
Publications
21,602
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1,654
Citations
Citations since 2016
45 Research Items
1018 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
Introduction

Publications

Publications (177)
Article
Full-text available
Lignocellulosic residues represent a low cost, high quality, and abundantly available alternative carbon sources that offer potential solutions to challenges linked to the growing demand for sustainability. In this study, the potential for the production of holocellulolytic enzymes induced by Paecilomyces formosus with agro-industrial coffee residu...
Article
Full-text available
Banana (Musa spp.), which is one of the world’s most popular and most traded fruits, is highly susceptible to pests and diseases. Pseudocercospora musae, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of Musa spp., resulting in serious economic damage to cultivars in the Cavendish subgroup. The aim of this study was to c...
Article
Jatropha curcas is a model for biorefinery applications. Although J. curcas cake (JCC), a product of oil extraction, shows potential in animal feed, inactivation of toxic phorbol esters (PE) in residues is necessary. Biological detoxification offers promise for animal feed, as well as for edible mushroom and enzyme production. Here, strains of Aura...
Article
Full-text available
Lignocellulosic biomass is a raw material appropriate for obtaining a wide variety of value-added products through different technologies. In the oil palm agroindustry, only 10% of the total products are oils. The remaining 90% is represented by lignocellulosic biomass and effluents. As these residual materials have enormous potential to produce bi...
Article
Full-text available
Spent mushroom Substrate is the by-product generated at the end of the mushroom growing cycle. It can be used in agriculture for different purposes, including seedling production, soil conditioning or application as an organic fertilizer. Tomato is one of the world́s most important crops, requiring considerable care, in terms of both nutrition and...
Article
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Oilseed plants such as cotton (Gossypium sp.) generate abundant biomass residues which contain significant levels of edible oil, crude proteins and other desirable biomolecules for the animal nutrition industry. The application of cottonseed cake in animal feed, a by-product of the cotton industry, is limited due to the natural presence of toxic fr...
Preprint
Full-text available
Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield across all growing regions. In 2017-18 and 2018-19 growing seasons, ca. 300 fungal samples were collected from seven Brazilian states. Hyphal tip cultures were obtained for DNA extraction and amplification of partial sequences of...
Article
Full-text available
Bananas are an important staple food crop in tropical and subtropical regions in Asia, sub-Saharan Africa, and Central and South America. The plant is affected by numerous diseases, with the fungal leaf disease black Sigatoka, caused by Mycosphaerella fijiensis Morelet [anamorph: Pseudocercospora fijiensis (Morelet) Deighton], considered one of the...
Article
Full-text available
Plant-parasitic nematodes cause extensive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. Chemical methods that have been deployed to control nematodes have largely been banned from use due to their p...
Preprint
Full-text available
Plant-parasitic nematodes cause expressive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. The chemical methods that have been deployed to control nematodes were largely banned from use due to their p...
Article
Full-text available
Given the global abundance of plant biomass residues, potential exists in biorefinery-based applications with lignocellulolytic fungi. Frequently isolated from agricultural cellulosic materials, Aspergillus terreus is a fungus efficient in secretion of commercial enzymes such as cellulases, xylanases and phytases. In the context of biomass sacchari...
Article
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Aspergillus tamarii grows abundantly in naturally composting waste fibers of the textile industry and has a great potential in biomass decomposition. Amongst the key (hemi)cellulose-active enzymes in the secretomes of biomass-degrading fungi are the lytic polysaccharide monooxygenases (LPMOs). By catalyzing oxidative cleavage of glycoside bonds, LP...
Article
Full-text available
Pseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grand...
Article
Arachis stenosperma is a wild peanut relative exclusive to South America that harbors high levels of resistance against several pathogens, including the peanut root-knot nematode (RKN) Meloidogyne arenaria. In this study, a proteomic survey of A. stenosperma-M. arenaria interaction using 2-DE and LC-MS/MS identified approximately 1400 proteins, out...
Article
Full-text available
Leaf pathogens are limiting factors in banana (Musa spp.) production, with Pseudocercospora spp. responsible for the important Sigatoka disease complex. In order to investigate cellular processes and genes involved in host defence responses, quantitative real-time PCR (RT-qPCR) is an analytical technique for gene expression quantifcation. Reliable...
Article
Species of Phaeochorella are biotrophic leaf parasites with a tropical distribution, traditionally accepted in the family Phyllachoraceae, Phyllachorales in classifications based on morphological characters. Phylogenetic evidence presented here resolves the relationship of Phaeochorella within the Sordariomycetes, based on a multilocus analysis of...
Chapter
Full-text available
Jatropha curcas, a tropical and subtropical shrub/tree, has been emerging as a promising biodiesel crop because of its high oil content and ability to grow in marginal lands. The kernel cake is the major by-product of the Jatropha biodiesel chain, rich in protein and has potential to be used in livestock feed; however, the presence of anti-nutritio...
Article
Full-text available
Prior phylogenetic studies of rust fungi have shown the Phakopsoraceae as polyphyletic. However, most of the ca. 13 genera currently placed in Phakopsoraceae s.l. have not been the subject of phylogenetic analyses. In this study we examine the placement of several species of Crossopsora (Phakopsoraceae) from newly generated nuc 28S rDNA (28S) seque...
Article
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The production of bioethanol from non-food agricultural residues represents an alternative energy source to fossil fuels for incorporation into the world's economy. Within the context of bioconversion of plant biomass into renewable energy using improved enzymatic cocktails, Illumina RNA-seq transcriptome profiling was conducted on a strain of Aspe...
Article
Peanut wild relatives (Arachis spp.) have high genetic diversity and are important sources of resistance to biotic and abiotic stresses. In this study, proteins were analyzed in root tissues of A. duranensis submitted to a progressive water deficit in soil and the differential abundance was compared to transcript expression profiles obtained by RNA...
Article
Full-text available
Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonsee...
Article
Apiosphaeria guaranitica, the causal agent of brown crust disease of several bignoniaceous hosts, among them Handroanthus and Tabebuia species, has been traditionally placed in Phyllachoraceae, based exclusively on morphological studies, without supporting molecular evidence. Here we provide molecular data for the link between sexual and asexual st...
Article
Full-text available
Jatropha curcas is an important oilseed plant, with considerable potential in the development of biodiesel. Although Jatropha seed cake, the byproduct of oil extraction, is a residue rich in nitrogen, phosphorus, potassium, and carbon, with high protein content suitable for application in animal feed, the presence of toxic phorbol esters limits its...
Article
Full-text available
The commonweed Euphorbia hirta (Euphorbiaceae) grows widely across tropical and subtropical regions. In this study, plants infected by an Uromyces species displayed charac- teristic rust pustules delimited by a necrotic band with a reddish- brown border. Observation of leaf tissues revealed 74% of le- sions encircled by a row of dark brown setose a...
Chapter
Mycotoxins are toxic secondary metabolites produced by filamentous fungi, which are widespread and are common contaminants of a large variety of important agricultural commodities. In order to improve food safety and reduce economical losses caused by mycotoxin contamination, detection and identification of mycotoxigenic fungi are essential at crit...
Preprint
Thissimple and rapid chromatographic detection method was developed and validated in order to accurately quantify trace levels of free gossypol in different cotton materials, including cottonseed, cottonseed cake and cottonseed cake treated with macrofungi.
Article
Full-text available
The common weed Euphorbia hirta (Euphorbiaceae) grows widely across tropical and subtropical regions. In this study, plants infected by an Uromyces species displayed characteristic rust pustules delimited by a necrotic band with a reddish-brown border. Observation of leaf tissues revealed 74% of lesions encircled by a row of dark brown setose acerv...
Conference Paper
Full-text available
Oil palm (Elaeis guineensis) is responsible for the majority of the world's vegetable oil supply, with applicaton in cosmetic, pharmaceutical, food and biofuel industries. The susceptibility of this crop to a number of diseases represents a considerable constraint to production. The introgression of resistance genes into elite genotypes is an impor...
Article
Multienzymatic complexes with plant lignocellulose-degrading activities have recently been identified in filamentous fungi secretomes. Such complexes have potential biotechnological applications in the degradation of agro-industrial residues. Fungal species from the Clonostachys genus have been intensively investigated as biocontrol agents; however...
Article
Full-text available
Aflatoxins (AFs) are natural toxins produced as secondary fungal metabolites. Common in diverse commodities, the consumption of aflatoxin-contaminated foodstuffs have harmful effects on human health mainly due to its carcinogenic effects. There is considerable demand for the development of rapid, reliable and cost-effective screening tools, once re...
Article
Colletotrichum species are usually well characterized on commercial fruit, but understudied on exotic fruits. Syzygium jambos (Myrtaceae) is an ornamental and fruit tree from Southeast Asia that is now widespread in Brazil (†). In 2016, S. jambos fruits with anthracnose symptoms were observed distributed randomly on several individual trees on the...
Chapter
Lignocellulose represents one of the main sources of renewable energy for biofuel production. Within this context, sugarcane bagasse, which is a discarded by-product of sugarcane processing, is a rich source of lignin, hemicellulose, and cellulose. Since sugarcane bagasse is a complex lignocellulosic structure, its complete enzymatic hydrolysis req...
Article
Background: Plants are constantly exposed to evolving pathogens and pests, with crop losses representing a considerable threat to global food security. As pathogen evolution can overcome disease resistance that is conferred by individual plant resistance genes, an enhanced understanding of the plant immune system is necessary for the long-term dev...
Article
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We identified Botryosphaeriaceae species associated with gummosis on . Anacardium in Brazil. Isolates were sampled and identified on the basis morphology and phylogeny, through analysis of a partial translation elongation factor 1-α sequence, ribosomal DNA internal transcribed spacers, and β-tubulin gene sequence. Ten taxa were identified, namely,...
Article
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Background and aims: Endoparasitic root-knot nematodes (RKNs) (Meloidogyne spp.) cause considerable losses in banana (Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early...
Article
Aspergillus tamarii was cultivated in different textile wastes. Xylanases with high levels of enzymatic activity were produced from the second day of cultivation, with constant production for up to seven days. Two xylanases, Xyl-1 and Xyl-2, with a molecular mass of 35.5 and 22 kDa respectively, were isolated from the crude extract and purified by...
Article
Plant cell wall represents an important source of fermentable sugars for second generation bioethanol production. However, cellulosic biomass hydrolysis still is a bottleneck to bioethanol production in an efficient and low cost process. Thermophilic bacteria have been studied as a source of cellulolytic enzymes for cellulosic biomass deconstructio...
Article
Full-text available
The cultivation of genetically modified (GM) crops has grown rapidly worldwide. This has led to regulatory authorities implementing strict procedures to monitor and verify the presence and abundance of GM varieties in agricultural crops. Immunochromatographic strip tests have been employed for detection of transgenic proteins expressed in GM crops...
Article
Full-text available
Among the diseases affecting banana (Musa sp), yellow Sigatoka, caused by the fungal pathogen Mycosphaerella musicola Leach, is considered one of the most important in Brazil, causing losses throughout the year. Understanding the genetic structure of pathogen populations will provide insight into the life history of pathogens, including the evoluti...
Article
Full-text available
An endo-β-1,4-xylanase (X22) was purified from crude extract of Emericella nidulans when cultivated on submerged fermentation using sugarcane bagasse as the carbon source. The purified protein was identified by mass spectrometry and was most active at pH and temperature intervals of 5.0-6.5 and 50-60°C, respectively. The enzyme showed half-lives of...
Article
Full-text available
Banana (Musa spp.) is one of the world?s most important monocotyledonous crops, cultivated in over 100 tropical and subtropical countries. As the world's most consumed fruit, it can represent a source of livelihood in countries of the African continent, where the annual per capita consumption may reach 400 kg. Brazil is responsible for approximatel...
Chapter
Myxobacteria are a group of eubacteria classified in the order Myxococcales, further subdivided into two suborders and eight families. These unique microorganisms exhibit distinctive social behavior and morphogenetic characteristics, such as the ability to form myxospores and fruiting bodies. Cellular aggregation is an important feature that allows...
Article
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Background Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this st...
Article
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The species of T. harzianum are well known for their biocontrol activity against plant pathogens. However, few studies have been conducted to further our understanding of its role as a biological control agent against S. sclerotiorum, a pathogen involved in several crop diseases around the world. In this study, we have used RNA-seq and quantitative...
Article
Filamentous fungi secrete a large amount of cellulose-degrading enzymes. Among them, fungi from Trichoderma genus are described as producers and secretors of a wide spectrum of cellulose-degrading enzymes. The biological deconstruction of cellulose within the biomass up to glucose monom is achieved through the synergistic action of multiple enzymes...
Article
The genus Trichoderma was first described in 1794 and presents a link to the sexual state Hypocrea. Whilst classification in the genus Trichoderma or Hypocrea using morphological characters is limited, an online identification tool was developed for accurate identification of all species within the genus Trichoderma. A group of Trichoderma species...
Article
Massively parallel pyrosequencing-based transcriptome analysis is an efficient approach for large-scale, functionally relevant, gene-derived SSR discovery. The objectives of this study were to identify SSR loci in unigene sequences generated using 454 transcriptome pyrosequencing, and to verify predicted gene function and in silico-derived differen...
Article
Sigatoka leaf spot, caused by the fungus Mycosphaerella musicola, significantly reduces leaf area and causes premature fruit ripening in banana. It is thus fundamentally important to develop genotypes resistant to fungal pathogens via crop improvement. In this context, transcriptional activity of NBS-LRR Resistance Gene Analogs (RGAs) was compared...
Article
The development of novel approaches for crop protection requires continued advances in our understanding of the molecular mechanisms controlling plant immunity. Molecular and genomics tools have advanced our understanding, with two key branches currently recognized. In one, plant innate defense is governed by PAMP-triggered immunity (PTI), followin...
Article
Full-text available
Background Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acum...
Article
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This paper presents results of two experiments for cultivation of Pleurotus ostreatus, Pleurotus pulmonarius and Pleurotus eryngii grown with different formulations of grass and straw mixtures derived from agro-industrial residues. Cultivation was prepared through a number of approaches, such as short composting/pasteurization and axenic culture. I...
Article
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In Rio de Janeiro State (Brazil), guava decline is a complex disease in which parasitism by Meloidogyne enterolobii predisposes Fusarium solani-immune trees to extensive root decay caused by this fungus. On the shoot, the symptoms include chlorosis, wilting, scorching of leaf margins and leaf drop, yield reduction, and plant death within months. Si...
Article
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Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly info...
Chapter
Full-text available
Banana is one of the most important subtropical crops. Its genetic system is relatively unknown and complicated by inter- and intraspecifi c hybridization, heterozygosity, and polyploidy. Thus molecular markers have been widely used among Musa germplasm to detect genetic variation and relationships, duplicate accessions identifi cation, monitoring...