Robert Miller

Robert Miller
United States Department of Agriculture | USDA · Agricultural Research Service (ARS)

Doctor of Philosophy

About

119
Publications
26,808
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4,779
Citations
Citations since 2017
32 Research Items
2306 Citations
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20172018201920202021202220230100200300400
Introduction
National Program Leader for Veterinary, Medical, and Urban Entomology (National Program 104) The mission of National Program 104 (NP 104) is to improve the protection of humans and livestock from blood-sucking arthropods, and from stinging, or otherwise damaging insects. NP 104 research is divided into three components: (1) Medical entomology for the public and military; (2) Veterinary entomology; and (3) Fire ants and other invasive ant pests.

Publications

Publications (119)
Article
The southern cattle fever tick (SCFT) Rhipicephalus (Boophilus) microplus, is considered the most important ectoparasite of livestock in the world because of high financial losses associated with direct feeding and transmission of the hemoparasites Babesia bovis, B. bigemina, and Anaplasma marginale. Unfortunately, SCFT in many parts of the world h...
Article
Full-text available
The aim of the present study was to evaluate in vitro and in vivo the acaricidal activity of two forms of geranium (Pelargonium graveolens) (PG). These two forms were the P. graveolens essential oil nanoemulsion (PGN), and the PG in combination with the sesame oil (SO), PGSO). These forms were first evaluated in vitro for their adulticidal, ovicida...
Article
Ectoparasitism result in annual losses to livestock producers estimated to reach billions of dollars in tropical and subtropical parts of the world where the southern cattle tick, Rhipicephalus (Boophilus) microplus, is established because this invasive pest is also a vector of pathogens causing bovine babesiosis and anaplasmosis. Reintroduction of...
Article
Full-text available
Ticks from the genus Rhipicephalus have enormous global economic impact as ectoparasites of cattle. Rhipicephalus microplus and Rhipicephalus annulatus are known to harbor infectious pathogens such as Babesia bovis, Babesia bigemina, and Anaplasma marginale. Having reference quality genomes of these ticks would advance research to identify druggabl...
Article
Full-text available
The invasive cattle fever tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae), occurs in the United States only along the Texas border as incursions from Mexico. Intensive acaricide treatment of cattle herds in Mexico to reduce tick populations has resulted in the development of resistance to an array of acaricides. Resistance...
Article
Pyrethroid pesticides are extensively used to manage animal and human disease vectors including the southern cattle tick Rhipicephalus microplus (Canestrini). The indiscriminate and incorrect use of pyrethroids has led to the almost ubiquitous development of resistance to this pesticide class for this tick species. Voltage-gated sodium channels (Na...
Article
The cattle tick, Rhipicephalus annulatus (Say) is a vector of bovine babesiosis and responsible for direct and indirect losses to cattle producing areas located in temperate and subtropical dry regions. Resistance against pyrethroids has been reported for this species in Asia and Africa, but never before in North America. An outbreak strain, Rio La...
Article
Full-text available
The objectives of this study were to evaluate the resistance of Amblyomma mixtum and Rhipicephalus microplus ticks from co-infested bovines from the Veracruz region in Mexico to different acaricide families and to demonstrate the viability of the packet test on different A. mixtum instars. The following acaricide families were used: a combination (...
Article
Intensive use of chemical acaricides for the control of cattle ticks (Rhipicephalus microplus) has led to the development of multiple acaricide resistance in Colombia. The present study aimed to characterize, using toxicological bioassays and molecular biology techniques, the resistance profile of a tick strain isolated from the Arauca state, North...
Article
Full-text available
The cattle tick, Rhipicephalus microplus, is a serious pest of cattle, with significant economic consequences to the livestock industries of tropical and semitropical countries. Rhipicephalus microplus belongs to the Metastriata group of the Ixodidae family known as hard ticks. When adult hard ticks feed, mating has not yet occurred and an initial...
Article
Full-text available
Leptospirosis, an emerging infectious disease caused by bacteria of the genus Leptospira, is thought to be the most widespread zoonotic disease in the world. A first step in preventing the spread of Leptospira is delineating the animal reservoirs that maintain and disperse the bacteria. Quantitative PCR (qPCR) methods targeting the LipL32 gene were...
Article
Full-text available
Background The southern cattle fever tick (SCFT), Rhipicephalus (Boophilus) microplus, remains endemic in Puerto Rico. Systematic treatment programmes greatly reduced and even eradicated temporarily this tick from the island. However, a systemic treatment programme that includes integrated management practices for livestock against SCFT remains to...
Article
Full-text available
The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, is the most economically important ectoparasite of cattle worldwide. A limitation for sustainable control and eradication is the emergence of acaricide resistance among tick populations. Molecular diagnostic tools offer the opportunity to detect resistance rapidly, which can be co...
Article
Comparative efficacy of infective juveniles of Steinernema riobrave Cabanillas, Poinar, and Raulston (355 strain), S. carpocapsae (Weiser) (All strain), S. feltiae Filipjev (SN strain), Heterorhabditis bacteriophora Poinar (VS strain), H. indica Poinar, Karunakar, and David (HOM1 strain), and H. floridensis Nguyen, Gozel, Koppenhöfer, and Adams (K2...
Chapter
Full-text available
Rhipicephalus (Boophilus) microplus and Rhipicephalus annulatus are invasive tick species and vectors of microbes causing bovine babesiosis and anaplasmosis that were declared eradicated from the USA in 1943 through efforts of the Cattle Fever Tick Eradication Program. These tick disease vectors remain established and affect livestock health and pr...
Article
Entomopathogenic nematodes are a promising alternative for tick control, especially to protect wildlife. Two entomopathogenic nematode species Steinernema riobrave Cabanillas, Poinar, and Raulston and Heterorhabditis floridensis Nguyen, Gozel, Koppenhöfer, and Adams were tested for in-vivo efficacy against southern cattle fever tick, Rhipicephalus...
Poster
Full-text available
The southern cattle tick, Rhipicephalus microplus, is one of the most damaging pests that parasites cattle in tropical and subtropical regions of the world. For its control, several chemical groups have been used, including fipronil. Fipronil and cyclodienes exert action by blocking GABA-gated chloride channels (GABA-Cl) present in the nervous syst...
Article
The southern cattle fever tick, Rhipicephalus microplus Canestrini, is widely distributed in tropical and subtropical regions, causing huge economic losses to milk and meat production. Application of acaricides is the most widely used control strategy, but because of increasing evidence of resistance to acaricide and environmental concerns, novel s...
Article
Immersion was used to test adults of six species of entomopathogenic nematodes for their effects on virulence and reproduction of engorged females of southern cattle fever tick, Rhipicephalus microplus (Canestrini) (Deutch strain). The nematodes were in the genera Steinernema (Rhabditida: Steinernematidae)-Steinernema carpocapsae (Weiser) (All stra...
Conference Paper
The southern cattle fever tick (CFT), Rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock, principally cattle, worldwide. The U.S. officially declared the eradication of this invasive tick species in 1943. However, outbreaks still occur in the Permanent Quarantine Zone spanning a region in south Texas t...
Article
Full-text available
The brown dog tick, Rhipicephalus sanguineus sensu lato (Latreille), is a cosmopolitan ectoparasite and vector of pathogens that kill humans and animals. Pyrethroids represent a class of synthetic acaricides that have been used intensely to try to control the brown dog tick and mitigate the risk of tick-borne disease transmission. However, acaricid...
Article
Full-text available
The brown dog tick, Rhipicephalus sanguineus (Latrielle) sensu lato, is an important ectoparasite of dogs and occasionally humans, capable of transmitting several pathogens, such as Rickettsia and Ehrlichia, which are of veterinary and medical importance. The brown dog tick is distributed worldwide and has an affinity for human habitations in much...
Article
Full-text available
Rhipicephalus sanguineus (Latreille) (Ixodida: Ixodidae) is a three-host dog tick found worldwide that is able to complete its' entire lifecycle indoors. Options for the management of R. sanguineus are limited and its' control relies largely on only a few acaricidal active ingredients. Previous studies have confirmed permethrin resistance and fipro...
Article
The genome of the cattle tick Rhipicephalus microplus, an ectoparasite with global distribution, is estimated to be 7.1 Gbp in length and consists of approximately 70% repetitive DNA. We report the draft assembly of a tick genome that utilized a hybrid sequencing and assembly approach to capture the repetitive fractions of the genome. Our hybrid ap...
Poster
Full-text available
The southern cattle fever tick (CFT), Rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock, principally cattle, worldwide. The U.S. officially declared the eradication of this invasive tick species in 1943. However, outbreaks still occur in the Permanent Quarantine Zone spanning a region in south Texas t...
Article
Full-text available
The effect of lemon oil (Citrus limon) on Sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. The mite samples were collected from naturally infected rabbits. The lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro application was done in five replicates for each co...
Article
Plant-derived natural products can serve as an alternative to synthetic compounds for control of ticks of veterinary and medical importance. Lippia gracilis is an aromatic plant that produces essential oil with high content of carvacrol and thymol monoterpenes. These monoterpenes have high acaricidal activity against Rhipicephalus (Boophilus) micro...
Conference Paper
Full-text available
A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14–15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC).
Article
Rhipicephalus microplus, the cattle fever tick, is a global economic problem to the cattle industry due to direct infestation of cattle and pathogens transmitted during feeding. Cattle fever tick outbreaks continue to occur along the Mexico-US border even though the tick has been eradicated from the USA. The organophosphate (OP) coumaphos targets a...
Article
Permethrin is a commonly used acaricide for tick control on domestic animals and in residential environments, while fipronil use is restricted to on-animal treatment. Following widespread reports of permethrin and fipronil application failures to control indoor infestations of Rhipicephalus sanguineus (Latreille), the brown dog tick, 31 tick popula...
Article
Full-text available
Two distant Antioquian cattle farms where systemic and topical acaricides had previously failed to control infestations by Rhipicephalus (Boophilus) microplus were studied. An initial in vivo study was conducted using single subcutaneous injections with a long-acting formulation of ivermectin (630 μg/kg). Injections were made at 3-month intervals o...
Conference Paper
Rhipicephalus microplus is an invasive tick that vectors Babesia bovis and B. bigemina, the protozoans that cause cattle fever. Acaricide resistant R. microplus populations are a major problem for many cattle producing areas of the world. Pyrethroid resistance in arthropods is typically based on mutations in domains I, II, and III of voltage-gated...
Conference Paper
Bovine babesiosis and anaplasmosis are deadly cattle diseases caused by microorganisms transmitted by the southern cattle fever tick (SCFT), Rhipicephalus (Boophilus) microplus, which is considered the most economically important ectoparasite of livestock worldwide. Humans brought animals infested with the SCFT to Puerto Rico (PR). Eradication effo...
Article
Full-text available
Puerto Rico (PR) is infested with the southern cattle fever tick (CFT), Rhipicephalus (Boophilus) microplus, which is considered the most economically important external parasite of livestock worldwide. A research coalition involving the livestock industry in PR, the PR Department of Agriculture (PR-DA), and the United States Department of Agricult...
Article
Full-text available
Background Vaccination as a control method against the cattle tick, Rhipicephalus (Boophilus) microplus has been practiced since the introduction of two products in the mid-1990s. There is a need for a vaccine that could provide effective control of R. microplus in a more consistent fashion than existing products. During our transcriptome studies o...
Article
Full-text available
Background Acaricide resistant Rhipicephalus microplus populations have become a major problem for many cattle producing areas of the world. Pyrethroid resistance in arthropods is typically associated with mutations in domains I, II, III, and IV of voltage-gated sodium channel genes. In R. microplus, known resistance mutations include a domain II c...
Conference Paper
Full-text available
BACKGROUND: Ticks and the diseases they transmit cause great economic losses to livestock in tropical countries. Non-chemical control alternatives include the use of resistant cattle breeds, biological control, farm management, and anti-tick vaccines. Chemical acaricides have played an essential role, and are used traditionally to control infestati...
Conference Paper
Full-text available
Dialelic crosses and backcrosses of pyrethroid resistant (RR) and susceptible (SS) Rhipicephalus (Boophilus) microplus tick strains were carried out and the substitution (Phe-Ile) within the sodium channel gene was monitored in order to analyze the effects of the genotype on the pyrethroid resistance phenotype as measured by the larval packet test...
Conference Paper
Full-text available
BACKGROUND: The southern cattle fever tick (SCFT), Rhipicephalus microplus, is considered the most economically important external parasite of livestock worldwide. SCFT populations resistant to acaricides complicate efforts to enhance the productivity of livestock. Here, acaricide resistance is summarized and integrated approaches in the Americas t...
Article
Full-text available
Rhipicephalus (Boophilus) microplus is a highly-invasive tick that transmits the cattle parasites (Babesia bovis and B. bigemina) that cause cattle fever. R. microplus and Babesia are endemic in Mexico and ticks persist in the United States inside a narrow tick eradication quarantine area (TEQA) along the Rio Grande. This containment area is threat...
Article
Full-text available
Ticks and the diseases they transmit cause great economic losses to livestock in tropical countries. Non-chemical control alternatives include the use of resistant cattle breeds, biological control and vaccines. However, the most widely used method is the application of different chemical classes of acaricides and macrocyclic lactones. Populations...
Article
In the Neotropics the control of tick infestations in red deer (Cervus elaphus) is achieved primarily through the use of acaricides and macrocyclic lactones. In Mexico, resistance to one or multiple classes of acaricides has been reported in Rhipicephalus microplus infesting cattle, but information on acaricide susceptibility in R. microplus infest...
Conference Paper
Cattle Fever Ticks Rhipicephalus (Boophilus) microplus and R.(B). annulatus, were eradicated from the U.S. but regularly make incursions along the border with Mexico. The USDA maintains a quarantine buffer zone with surveillance for stray Mexican cattle and inspection of herds in the counties along the Rio Grande. The year 2008, a peak El Nino osci...
Conference Paper
Full-text available
Acaricide resistance mechanisms in Rhipicephalus sanguineus Amanda L. Eiden1, Phillip E. Kaufman1, Faith M. Oi1, Michael J. Dark2,3 and Robert Miller4 1Entomology and Nematology Dept., University of Florida, Gainesville, FL 2College of Veterinary Medicine, University of Florida, Gainesville, FL 3Emerging Pathogens Institute, University of F...
Conference Paper
The southern cattle tick (Rhipicephalus (Boophilus) microplus) is a hematophagous external parasite that vectors the causative agents of bovine babesiosis or cattle tick fever, Babesia bovis and B. bigemina, and anaplasmosis, Anaplasma marginale. The southern cattle tick is a threat to the livestock industry in many locations throughout the world....
Article
Efficacy of an organophosphate (OP) mixture acaricide, Ravap®(Bayer, Shawnee, KS) was evaluated as a spray at 0.15 and 0.3% active ingredient (Al) on cattle infested with all parasitic stages of OP-resistant Rhipicephalus (Boophilus) microplus (Canestrini). Laboratory bioassays showed ticks were 18.7X more resistant to OP acaricides than a suscepti...
Article
Full-text available
Resistance to synthetic pyrethroids (SP) in the cattle tick Rhipicephalus (Boophilus) microplus is widespread throughout its distribution area. Three single nucleotide substitutions identified in Domains II and III of the sodium channel gene of R. (B.) microplus are known to be associated with target site pyrethroid resistance. We developed a multi...
Conference Paper
Determination of acaricide resistance in Rhipicephalus sanguineus and evaluation of resistance mechanisms Amanda L. Eiden1, Phillip E. Kaufman1, Faith M. Oi1, Michael J. Dark2,3 and Robert Miller4 1Entomology and Nematology Dept., University of Florida, Gainesville, FL 2College of Veterinary Medicine, University of Florida, Gainesville, FL 3Emergin...
Article
Five strains of Rhipicephalus (Boophilus) microplus collected from Tamaulipas Mexico were tested for resistance against several classes of acaricides commonly used. All were resistant to fipronil. Four of five were co-resistant to permethrin and coumaphos in addition to being resistant to fipronil. One strain, El Zamora was found multi-resistant to...
Article
Full-text available
The ticks Rhipicephalus (Boophilus) annulatus and R. (B.) microplus, commonly known as cattle and southern cattle tick, respectively, impede the development and sustainability of livestock industries throughout tropical and other world regions. They affect animal productivity and wellbeing directly through their obligate blood-feeding habit and ind...
Article
Bovine babesiosis, also known as cattle fever, is a tick-borne protozoal disease foreign to the United States. It was eradicated by eliminating the vector species, Rhipicephalus (Boophilus) annulatus and Rhipicephalus (Boophilus) microplus, through the efforts of the Cattle Fever Tick Eradication Program (CFTEP), with the exception of a permanent q...
Article
The cattle tick, Rhipicephalus microplus, is arguably the world's most economically important external parasite of cattle. Sustainable cattle tick control strategies are required to maximise the productivity of cattle in both large production operations and small family farms. Commercially available synthetic acaricides are commonly used in control...
Article
The Rhipicephalus microplus genome is large and complex in structure, making it difficult to assemble a genome sequence and costly to resource the required bioinformatics. In light of this, a consortium of international collaborators was formed to pool resources to begin sequencing this genome. We have acquired and assembled genomic DNA into contig...
Article
Full-text available
This study was conducted to obtain an epidemiological view of acaricide resistance in populations of Rhipicephalus (Boophilus) microplus in the State of Mato Grosso do Sul. Twenty-four tick samples were collected from municipalities in the State where farmers had reported concerns about resistance to or failure of tick control. These ticks were sub...
Article
Full-text available
Dialelic crosses and backcrosses of pyrethroid resistant (RR) and susceptible (SS) Rhipicephalus (Boophilus) microplus tick strains were carried out and the substitution (Phe-Ile) within the sodium channel gene was monitored in order to analyze the effects of the genotype on the pyrethroid resistance phenotype as measured by the larval packet test...
Article
Full-text available
The therapeutic and persistent efficacy of a single subcutaneous injection of a long-acting formulation of moxidectin at a concentration of 1 mg/kg body weight was determined against Rhipicephalus (Boophilus) microplus (Canestrini), along with the concentration-time blood sera profile in treated cattle. The therapeutic efficacy against ticks of all...
Article
Full-text available
Various acaricide-resistant strains of Rhipicephalus (Boophilus) microplus, representative of the major resistance mechanisms found in Mexico and Brazil, were exposed to spinosad using the Food and Agriculture Organization of the United Nations larval packet test and adult immersion test (AIT). Larvae of all strains tested were found to be suscepti...
Article
Full-text available
Synergistic effects of verbutin, a member of aryl alkynyl derivatives, to three commonly used acaricides were evaluated with the modified Food and Agricultural Organization Larval Packet Test (FAO-LPT) against both susceptible and resistant strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae). Thes...
Article
A farmlet study was conducted over 4 years in which three treatments were applied to six groups of Holstein dairy calves. Calves in each group were infested with equal numbers of N-strain (susceptible) and Ultimo strain (amitraz and synthetic pyrethroid resistant) tick larvae to establish self-sustaining populations with an initial, measurable leve...