Robert Lenartowski

Robert Lenartowski
Nicolaus Copernicus University | umk · Departament of Cellular and Molecular Biology

Ph.D.

About

30
Publications
5,779
Reads
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234
Citations
Citations since 2016
12 Research Items
153 Citations
201620172018201920202021202205101520253035
201620172018201920202021202205101520253035
201620172018201920202021202205101520253035
201620172018201920202021202205101520253035
Introduction
Robert Lenartowski currently works in Department of Cellular and Molecular Biology, Nicolaus Copernicus University. Robert does research in Molecular Biology, Genetics and Cell Biology. Their most recent publication is 'Myosin VI in the nucleus of neurosecretory PC12 Cells : Stimulation-dependent nuclear translocation and interaction with nuclear proteins'.
Additional affiliations
September 2019 - present
Nicolaus Copernicus University
Position
  • Professor
Education
September 1995 - October 2001
Faculty of Biology and Earth Sciences, NCU in Toruń
Field of study
  • molecular biology
September 1990 - June 1995
Faculty of Biology and Earth Sciences, Nicolaus Copernicus University (NCU) in Toruń
Field of study
  • molecular biology

Publications

Publications (30)
Article
Full-text available
Pollen tube growth depends on several complex processes, including exo/endocytosis, cell wall biogenesis, intracellular transport, and cell signaling. Our previous results provided evidence that calreticulin (CRT)—a prominent calcium (Ca2+)-buffering molecular chaperone in the endoplasmic reticulum (ER) lumen—is involved in pollen tube formation an...
Article
Full-text available
Background Pollen development in the anther in angiosperms depends on complicated cellular interactions associated with the expression of gametophytic and sporophytic genes which control fundamental processes during microsporo/gametogenesis, such as exo/endocytosis, intracellular transport, cell signaling, chromatin remodeling, and cell division. M...
Article
Full-text available
The transcriptional and posttranscriptional AGO-mediated control of gene expression may play important roles during male monocot gametophyte development. In this report, we demonstrated dynamic changes in the spatiotemporal distribution of AGO1 and AGO4, which are key proteins of the RNA-induced silencing complex (RISC) in Hyacinthus orientalis mal...
Article
Full-text available
Myosin VI (MYO6) is an actin-based motor that has been implicated in a wide range of cellular processes, including endocytosis and the regulation of actin dynamics. MYO6 is crucial for actin/membrane remodeling during the final step of Drosophila spermatogenesis, and MYO6-deficient males are sterile. This protein also localizes to actin-rich struct...
Article
Calreticulin (CRT) is a multifunctional resident endoplasmic reticulum (ER) luminal protein implicated in regulating a variety of cellular processes, including Ca⁠2+ storage/mobilization and protein folding. These multiple functions may be carried out by different CRT genes and protein isoforms. The plant CRT family consist of three genes: CRT1 and...
Article
Full-text available
Myosin VI (MVI) is a unique actin-based motor protein moving towards the minus end of actin filaments, in the opposite direction than other known myosins. Besides well described functions of MVI in endocytosis and maintenance of Golgi apparatus, there are few reports showing its involvement in transcription. We previously demonstrated that in neuro...
Article
Full-text available
Calcium (Ca(2+)) plays essential roles in generative reproduction of angiosperms, but the sites and mechanisms of Ca(2+) storage and mobilization during pollen-pistil interactions have not been fully defined. Both external and internal Ca(2+) stores are likely important during male gametophyte communication with the sporophytic and gametophytic cel...
Article
Full-text available
Myosin VI (MVI) is a versatile actin-based motor protein that has been implicated in a variety of different cellular processes, including endo- and exocytic vesicle trafficking, Golgi morphology, and actin structure stabilization. A role for MVI in crucial actin-based processes involved in sperm maturation was demonstrated in Drosophila. Because of...
Article
Full-text available
Main conclusion: Calreticulin is involved in stabilization of the tip-focused Ca (2+) gradient and the actin cytoskeleton arrangement and function that is required for several key processes driving Petunia pollen tube tip growth. Although the precise mechanism is unclear, stabilization of a tip-focused calcium (Ca(2+)) gradient seems to be critica...
Article
Full-text available
The enzyme DOPA decarboxylase (aromatic-L-amino-acid decarboxylase, DDC) plays an important role in the dopaminergic system and participates in the uptake and decarboxylation of amine precursors in the peripheral tissues. Apart from catecholamines, DDC catalyses the biosynthesis of serotonin and trace amines. It has been shown that the DDC amino ac...
Article
Full-text available
The nuclear matrix (NM), or nuclear skeleton, is the non-chromatin, ribonucleoproteinaceous framework that is resistant to high ionic strength buffers, nonionic detergents, and nucleolytic enzymes. The NM fulfills a structural role in eukaryotic cells and is responsible for maintaining the shape of the nucleus and the spatial organization of chroma...
Article
Full-text available
Key message: Calreticulin expression is upregulated during sexual reproduction of Hyacinthus orientalis , and the protein is localized both in the cytoplasm and a highly specialized cell wall within the female gametophyte. Several evidences indicate calreticulin (CRT) as an important calcium (Ca(2+))-binding protein that is involved in the generat...
Article
Full-text available
Key message In germinating pollen grains and growing pollen tubes, CRT is translated on ER membrane-bound ribosomes in the regions where its activity is required for stabilization of tip-focused Ca 2+ gradient. Abstract Pollen tube growth requires coordination of signaling, exocytosis, and actin cytoskeletal organization. Many of these processes ar...
Article
Full-text available
Calcium (Ca(2+)) plays essential roles in plant sexual reproduction, but the sites and the mechanism of Ca(2+) mobile storage during pollen-pistil interactions have not been fully defined. Because the Ca(2+)-buffering protein calreticulin (CRT) is able to bind and sequester Ca(2+), it can serve as a mobile intracellular store of easily releasable C...
Conference Paper
Full-text available
We report the discovery of a new mechanical transition in stretched and topologically constrained DNA. We used a well established technique of stretching single molecules with an untreated Atomic Force Microscope tip to extend DNA. We explored the DNA mechanics under previously untested high stretching force regime, up to 1.8 nN of possible limit o...
Article
Full-text available
We show results of our high force (up to 1.8 nN) atomic force microscopy force spectroscopy measurements of a double stranded DNA. We have found that the force spectra of torsionally constrained molecules display a small plateau occurring at a force of approximately 1 nN. This transition is absent in molecules with rotational freedom. Based on all-...
Article
Full-text available
Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca(2+)-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca(2+) storage and release, protein synthesis, and molecular chaperoning in both animals and plants. CRT has long been suggest...
Article
Full-text available
The activity of tyrosine hydroxylase (TH, EC 1.14.16.2) gene and protein determines the catecholamine level, which, in turn, is crucial for the organism homeostasis. The TH gene expression is regulated by near all possible regulatory mechanisms on epigenetic, transcriptional and posttranscriptional levels. Ongoing molecular characteristic of the TH...
Article
In this report, the distributions of calreticulin (CRT) and its transcripts in Haemanthus pollen, pollen tubes, and somatic cells of the hollow pistil were studied. Immunoblot analysis of protein extracts from mature anthers, dry and germinated pollen, growing pollen tubes, and unpollinated/pollinated pistils revealed a strong expression of CRT. Bo...
Article
Full-text available
Calreticulin (CRT) is an ubiquitously expressed Ca2+ binding protein in endoplasmic reticulum (ER) of eukaryotic cells. A highly conserved structure between CRTs from different species of animals and plants confirms an important role of the protein in living cells. CRT has been found in different cellular compartments, suggesting to play a role in...
Article
Full-text available
Selective protein degradation depends on their quality being controlled by the cellular system, which includes chaperones involved in protein folding and two degradation systems, the proteasomal and lysosomal. CHIP (carboxyl terminus of Hsp70-interacting protein), an E3 ubiquitin ligase and co-chaperone, serves as a chaperone-degradation system int...
Article
Full-text available
Recently we have shown that in vitro binding of the proximal part of the human tyrosine hydroxylase gene to the nuclear matrix is correlated with its transcriptional activity. The strongest binding potential was predicted by computing for the first intron sequence (Lenartowski & Goc, 2002, Neurosci Lett.; 330: 151-154). In this study a 16 kb fragme...
Article
Full-text available
Association of the human tyrosine hydroxylase (TH) gene with the nuclear matrix was studied using bovine matrices from tissues differing in expression of the gene. An in vitro binding assay was performed with fragments encompassing the -2300/+2300 region of the human TH gene. All fragments bound to the nuclear matrix from the adrenal medulla but no...
Article
Full-text available
The tyrosine hydroxylase gene is subject to very precise regulation which aim is to adjust the level of the catecholamines to current stimuli disturbing the homeostasis. The fine tuning of the TH gene activity is realized by the "cross-talk" between the complexes of transcriptional factors with their appropriate regulatory sequences. The transcript...
Article
Full-text available
The tyrosine hydroxylase plays a key role in the catecholamine synthesis responding to many stimuli disturbing homeostasis. In this part we described the posttranscriptional levels of the gene and the protein regulation including: alternative splicing, isoform specificity, feedback inhibition, protein phosphorylation and dephosphorylation, protein...
Article
The objective of our research on Petunia hybrida is to understand the role of calreticulin in the growth of pollen tubes in the pistil. The aim of this study was the first step: finding out whether CRT gene expression takes place in unpollinated and pollinated styles. It was revealed by in situ hybridization that the transcription of the calreticul...

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