Rob Keller

Rob Keller
Charlemagne College, Nijmegen, The Netherlands · Chemistry

PhD MSc MEd

About

52
Publications
198,925
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488
Citations
Introduction
After years working as product manager in the field of ingredients, Rob Keller currently works at the Section Chemistry, Charlemagne College, Nijmegen, The Netherlands. Rob does research in Physical Chemistry, Materials Chemistry and Biochemistry. The most recent publication is 'Identification of Possible Lipid Binding Regions in Food Proteins and Peptides and Additional In Silico Analysis' in Food Biophysics.
Research Experience
September 1995 - May 1998
Wageningen University & Research
Position
  • Senior Researcher
Description
  • At the former ATO-DLO institute, currently part of Wageningen University. Department of Agricultural ingredients.
August 1994 - August 1995
TNO
Position
  • Researcher
Description
  • TNO Food in Zeist. Working at the Department of Cereal Science.
August 1993 - April 1994
Max Planck Institute for Biophysical Chemistry
Position
  • PhD Student
Description
  • Frequent visits for 1-2 months, in total round half a year. Used the state-of-the-art ESR equipment and expertise of the group. As part of the PhD thesis research.
Education
February 2010 - September 2012
Hogeschool Fontys
Field of study
  • Chemistry
August 1984 - December 1989
Utrecht University
Field of study
  • Chemistry

Publications

Publications (52)
Article
Full-text available
The conformational consequences of the interaction of the PhoE signal peptide with bilayers of different types of phospholipids was investigated using circular dichroism. It was found that interaction of the signal peptide with anionic phospholipid vesicles of dioleoylphosphatidylglycerol and dioleoylphosphatidylserine results in induction of high...
Article
Full-text available
Protein translocation is an important cellular process. SecA is an essential protein component in the Sec system, as it contains the molecular motor that facilitates protein translocation. In this study, a bioinformatics approach was applied in the search for possible lipid-binding helix regions in protein translocation motor proteins. Novel lipid-...
Article
Full-text available
The Eisenberg plot or hydrophobic moment plot methodology is one of the most frequently used methods of bioinformatics. Bioinformatics is more and more recognized as a helpful tool in Life Sciences in general, and recent developments in approaches recognizing lipid binding regions in proteins are promising in this respect. In this study a bioinform...
Article
Full-text available
This study aimed to characterise the competitive adsorption behaviour of the different components present in wheat flour and emulsifiers used in breadmaking at an air–water interface using surface balance measurements. It was found that the proteins could be arranged in increasing order of surface activity: albumin < globulin < glutenin < gliadin....
Article
Full-text available
The role of protein-lipid interactions is increasingly recognized to be of importance in numerous biological processes. Bioinformatics is being increasingly used as a helpful tool in studying protein-lipid interactions. Especially recently developed approaches recognizing lipid binding regions in proteins can be implemented. In this study one of th...
Article
Full-text available
The phenomenon of hijacked journals can be seen as the ultimate example of predatory publishing. Though there are some initiatives to list such sad examples of fraudulent practices it is out of personal experience that from time-to-time new examples 'pop-up'. On numerous occasions, while participating on ResearchGate unfortunate experiences are sha...
Article
Full-text available
In an attempt to estimate the quality of an open access journal the indexing in the Scopus database is often perceived as a positive indicator. While the inclusion in the Beall's list is anticipated as at least a serious warning sign that the journal (or the publisher behind is) might be predatory. On numerous occasions it has been noticed that the...
Data
The data presented here are based on the complete list of predatory journals identified by: Macháček, V., Srholec, M. Predatory publishing in Scopus: evidence on cross-country differences. Scientometrics (2021). https://doi.org/10.1007/s11192-020-03852-4 as was found in 2017. Find enclosed a copy of the supplementary material belonging to the Machá...
Method
Full-text available
Examples of the use of bioinformatics with a focus on biochemistry.
Method
Full-text available
Some thoughts on whether the Beall's list tells you the 'whole' and 'indisputable' story when it comes to alleged predatory journals and publishers. Including a call for more consideration for researchers in this matter.
Method
Full-text available
Possible help on your way through the internet and all its search engines/databases and so on.
Data
This is the supplement as part of the publication: Keller, R. C. (2015). The role and significance of potential lipid-binding regions in the mitochondrial protein import motor: an in-depth in silico study. 3 Biotech, 5(6), 1041-1051. Included are: additional data and a brief overview of various hydrophobicity scales (see for discussion the indicate...
Article
Full-text available
The results of the search for potential helical lipid binding regions in a number of well-known food proteins is described. All selected food proteins have either well-described or strong indications of protein-lipid interaction features. The results are obtained with the aid of a number of selected bioinformatics tools. The identified potential li...
Article
In an era where sustainability becomes increasingly prominent it is clear that agricultural sources are playing an important role in this goal. Cereal science is a well-established research area when it comes to agricultural and food science related topics. Throughout the years a wealth of information has been gathered when it comes to cereals. Rec...
Article
Full-text available
Over the last two decades, an impressive progress has been made in the identification of novel factors in the translocation machineries of the mitochondrial protein import and their possible roles. The role of lipids and possible protein–lipids interactions remains a relatively unexplored territory. Investigating the role of potential lipid-binding...
Article
Full-text available
The presence of possible lipid-binding regions in the cytoplasmic or extracellular loops of membrane proteins with an emphasis on protein translocation membrane proteins was investigated in this study using bioinformatics. Recent developments in approaches recognizing lipid-binding regions in proteins were found to be promising. In this study a tot...
Thesis
Full-text available
Thesis as part of obtaining Master of Education degree. At Fontys University Applied Sciences, Tilburg, The Netherlands
Method
Full-text available
Project as part of the program in order to obtain a Master of Education degree. Performed at Applied Sciences University Fontys, Tilburg, the Netherlands.
Data
Full-text available
Chapter
Full-text available
Reversible physical crosslinks (junction zones) in gelatin gels or films are thought to consist of triple helical collagen-like helices. Using circular dichroism (CD) the structural elements present in gelatin have been studied. The recognition and quantification of the most important conformations in the preparations have been investigated using a...
Poster
Full-text available
Poster presentation at various conferences etc.
Article
Full-text available
The testing of new gelatin batches for photographic purposes is a frequently recurring and tedious task that would benefit from efficient new methods for quality assessment. One of the quality parameters is the efficiency of gelation and the stability and strength of the gel and final photographic matrix. The objective of the present IOP-Industrial...
Presentation
Full-text available
Lecture: An overview was presented of 2-2.5 years of research dedicated to understanding more about the quality parameters of gelatines.
Poster
Full-text available
Poster used on various conferences and meetings
Presentation
Full-text available
Presentation at the Polymer Networks 96 organized by the International Union of Pure and Applied Chemistry Polymer Networks Group in Doorn, the Netherlands, september 2-6, 1996.
Poster
Full-text available
Poster presentation at various conferences etc.
Article
Full-text available
The interactions between a bacterial precursor protein and phospholipids in bilayer-based model membrane systems is addressed in this study. The precursor-lipid interactions were assessed from the side of the lipid phase by fluorescence and electron spin resonance spectroscopy, using the precursor of the Escherichia coli outer membrane protein PhoE...
Article
Full-text available
To investigate in a direct way the interaction between a precursor protein and phospholipids, monolayer studies were performed using the purified precursor of Escherichia coli outer-membrane protein PhoE. It was demonstrated that prePhoE can insert efficiently into monolayers of dioleoylglycerophosphoglycerol (Ole2GroP Gro) and dioleoylglycerophosp...
Chapter
Full-text available
Reversible physical crosslinks (junction zones) in gelatin gels and films are thought to consist of triple helical collagen-like structures, however other structures may also occur. In an attempt to understand the relation between the material properties and the various junction structures and their abundance, we characterized these structures usin...
Article
Full-text available
In our search for suitable resonance energy transfer (RET) couples for studying protein-lipid interactions, the promising couple coumarin-BODIPY was found and characterized. We characterized the RET from the donor coumarin to two different dipyrrometheneboron difluoride (BODIPY)-labeled phospholipid analogs both experimentally and theoretically. Ca...
Article
Full-text available
The effects of SecA-lipid interactions on lipid mobility were studied by electron spin resonance (ESR) spectroscopy in bilayer systems containing phospholipids spin-labeled at different positions along the acyl chain. The SecA protein, which functions in protein translocation at the cytosolic side of the E. coli inner membrane, was found to decreas...
Article
Full-text available
Preliminary results of a study aimed to characterise the competitive adsorption behaviour of components present in wheat flour as used in breadmaking. Investigations at an air–water interface as studied by surface balance measurements. Final results are presented in: Keller, R. C. A., Orsel, R., & Hamer, R. J. (1997). Competitive adsorption behavio...
Thesis
Full-text available
Some details (and links to searchable and readable parts) of this thesis about the role of protein-lipid interactions in protein translocation.
Poster
Full-text available
Poster presented at various conferences.
Presentation
Full-text available
Lecture: Data were presented dealing with the role of the signal sequence in protein-lipid interactions and its possible meaning in protein translocation.
Article
Full-text available
SecA which is an overall acidic protein was found to induce an increase in the turbidity of a solution of vesicles consisting of negatively charged phospholipids. This increase was found to be due to an aggregation of the vesicles mediated by SecA. The SecA-mediated vesicle aggregation was not found for zwitterionic 1,2-dioleoyl-sn-glycero-3-phosph...
Poster
Full-text available
Poster presentation at various conferences etc.
Presentation
Full-text available
(Poster) Presentation used for various purposes like: The international symposium on cellular and molecular biology of phosphate and phosphorylated compounds in microorganisms, Wood Hole, Massachusetts, 12-17 September 1993.
Chapter
Full-text available
In membrane biogenesis and protein targeting, polypeptide chains very often insert into and move across biological membranes. Correct membrane assembly and functioning critically depends on the spatial and temporal interactions between the two main membrane components, i.e. proteins and lipids. It can therefore a priori be reasoned that protein-lip...
Method
Full-text available
Proteins which have to be translocated from the cytosol to another destination in the cell are synthesized with a N-terminal extension, the signal sequence. Although the signal sequence is essential for translocation of a great deal of proteins, its exact role is still unclear. The signal peptide-lipid interaction may be of great importance for the...
Article
Full-text available
The interaction of the signal peptide of the Escherichia coli outer membrane protein PhoE with different phospholipid vesicles was investigated by fluorescence techniques, using a synthetic mutant signal peptide in which valine at position -8 in the hydrophobic sequence was replaced by tryptophan. First it was established that this mutation in the...
Article
Full-text available
The luminescence of nominally pure BaLn2(MoO4)4 (Ln = La,Gd) is reported. Below 300 K molybdate emission is observed. The following activators were investigated: Eu3+, Tb3+, Pb2+ and Bi3+. The two lanthanide ions show their characteristic emission, the two s2 configuration ions give an emission in which the host-lattice group as well as the activat...

Questions

Questions (4)

Network

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Projects

Projects (8)
Archived project
Main subject: Fluorescence approach in protein translocation (Lab of prof. dr. B. de Kruijff). Secondary subject: Luminescence approach in solid state Chemistry as part of a module Physical Chemistry (Lab of prof. dr. G. Blasse)