Rita Sachse

Rita Sachse
Roche

Dr.

About

10
Publications
3,277
Reads
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311
Citations
Additional affiliations
November 2016 - November 2016
Roche
Position
  • Expert Start Up Scientist - System Software Integration

Publications

Publications (10)
Article
Full-text available
Membrane proteins are key elements in cell-mediated processes. In particular, G protein-coupled receptors (GPCRs) have attracted increasing interest since they affect cellular signaling. Furthermore, mutations in GPCRs can cause acquired and inheritable diseases. Up to date, there still exist a number of GPCRs that has not been structurally and fun...
Article
Full-text available
Difficult to express membrane proteins represent an increasing amount of therapeutic molecules. Considerable optimization is often required for downstream applications such as assay development and functional characterization. Cell-free systems emerged as powerful tools for the synthesis of structurally and functionally divergent membrane proteins....
Article
Full-text available
When taking up the gauntlet of studying membrane protein functionality, scientists are provided with a plethora of advantages, which can be exploited for the synthesis of these difficult-to-express proteins by utilizing cell-free protein synthesis systems. Due to their hydrophobicity, membrane proteins have exceptional demands regarding their envir...
Article
Full-text available
In this study, we present a novel technique for the synthesis of complex prokaryotic and eukaryotic proteins by using a continuous-exchange cell-free (CECF) protein synthesis system based on extracts from cultured insect cells. Our approach consists of two basic elements: First, protein synthesis is performed in insect cell lysates which harbor end...
Article
Full-text available
Incorporation of proteins in biomimetic giant unilamellar vesicles (GUVs) is one of the hallmarks towards cell models in which we strive to obtain a better mechanistic understanding of the manifold cellular processes. The reconstruction of transmembrane proteins, like receptors or channels, into GUVs is a special challenge. This procedure is essent...
Article
Full-text available
The main goal of cell-free protein synthesis is to produce correctly folded and functional proteins in reasonable amounts for further downstream applications. Especially for eukaryotic proteins, functionality is often directly linked to the presence of posttranslational modifications. Thus, it is of highest interest to develop novel cell-free expre...
Article
Full-text available
Cell-free protein synthesis (CFPS) is a valuable method for the fast expression of difficult-to-express proteins as well as posttranslationally modified proteins. Since cell-free systems circumvent possible cytotoxic effects caused by protein overexpres- sion in living cells, they significantly enlarge the scale and variety of proteins that can be cha...
Article
In this project we address the challenge of incorporation of transmembrane proteins with defined orientation in biomimetic giant unilamellar vesicles (GUVs). The reconstitution of biologically relevant transmembrane proteins, like receptors or channel proteins, into GUVs makes them easily accessible to biophysical investigation using patch-clamp or...

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