Rina Chakrabarti

Rina Chakrabarti
University of Delhi | DU · Department of Zoology (Faculty of Science)

Senior Professor University of Delhi

About

147
Publications
38,203
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2,569
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Introduction
Recirculating aquaculture: optimization of stocking density of larvae & characterization of ammonia oxidizing microbes. Abiotic stressors: Impact of temperature, dissolved oxygen, visible light & UV-B on the physiology of fishes and its mitigation through enriched diets. Evaluation of immunostimulatory and disease resistance properties of seeds & leaves of Achyranthes aspera in fish in ponds. Mass production of zooplankton using organic manures. Digestive enzyme profiles of carps during ontogenic development. Purification & characterization of trypsin from digestive systems of carps. Large scale production of duckeed Lemna minor & Spirodela polyrhiza in ponds. Formulation of pelleted diets with duckweeds using Twin-Screw Extruder & In vitro digestibility study of prepared diets.

Publications

Publications (147)
Article
Full-text available
The immunostimularory properties of Achyranthes aspera leaves and seeds supplemented feeds were evaluated in Asian catfish, Clarias batrachus (0.352±0.008 g). The experimental feeds contained 0.25% leaves (EFL1), 0.5% leaves (EFL2), 0.5% seeds (EFS) and 0% leaves or seeds (control feed; CF). In CF, leaves and seeds were absent. Fish were immunized...
Article
Full-text available
Ultraviolet B (UV-B) radiation is a potent environmental stressor and it severely affects the survival, growth, and physiology of aquatic organisms. The UV-B protective properties of three herbal ingredients enriched diets were evaluated in pre-exposed Catla catla. Fish (70.38 ± 1.18 g) were divided into five sets with three replicates each. Four s...
Article
The ultraviolet–B (UV–B) radiation is harmful to the aquatic organisms. The UV–B protective properties of leaves and seeds of herb Achyranthes aspera were evaluated in Clarias batrachus. Fish were fed with four diets—EFL1, EFL2 containing 0.25 and 0.5% leaves, EFS containing 0.5% seeds and control, CF. After 83 days of feeding, fish were exposed to...
Article
Full-text available
The freshwater macrophytes are abundant in tropical and subtropical climates. These macrophytes may be used as feed ingredients for fish and other animals. The nutritional value of twelve freshwater-cultured macrophytes was evaluated in the present study. Significantly higher crude protein (36.94-36.65%) and lipid (8.13-7.62%) were found in Lemna m...
Article
Full-text available
The potential nutritional value of duckweed Lemna minor (Lemnaceae) was evaluated for common carp Cyprinus carpio fry. Fish were fed diets containing five graded levels of duckweed: 0% (LM0, control), 5% (LM5), 10% (LM10), 15% (LM15) and 20% (LM20). The final weight and specific growth rate were significantly higher in LM15 and LM20 diets fed fish...
Article
Full-text available
The greater duckweed Spirodela polyrhiza (Lemnaceae) is a free-floating freshwater macrophyte. The effect of dietary inclusion of duckweed in the feed of common carp Cyprinus carpio fry was evaluated. The control feed (SP0) contained soybean meal as the primary protein source. In four experimental feeds, greater duckweed was incorporated at levels...
Article
Full-text available
The greater duckweed Spirodela polyrhiza (Lemnaceae) is a free-floating freshwater macrophyte. The effect of dietary inclusion of duckweed in the feed of common carp Cyprinus carpio fry was evaluated. The control feed (SP0) contained soybean meal as the primary protein source. In four experimental feeds, greater duckweed was incorporated at levels...
Article
Snow trout, Schizothorax richardsonii (Gray, 1832), is an economically important fish in the Himalayan region. Snow trout fry (118.53 ± 5.42 mg) were cultured in flow-through systems for 60 days. Fry were fed with four different diets containing: 0.08% vitamin C (D1), 0.5% Achyranthes aspera seeds (D2), both vitamin C and seeds (D3), and a control...
Article
Full-text available
A software tool named Interactive Fish Feed Designer (IFFD) has been developed using Java programming language for the formulation of fish feed. IFFD allows formulating fish feed with desirable protein and lipid levels using multiple ingredients. In the first step, ingredients are selected from a list. Then the ratio of the ingredients is adjusted...
Article
The present study was conducted to evaluate the effect of dietary inclusion of Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. Magur fry (0.51 ± 0.032 g) were cultured in hapas set inside a pond and were fed with three feeds. Two experimental feeds FS1 and...
Article
Full-text available
The nutritional value of seeds and leaves of Achyranthes aspera as fish feed ingredients was evaluated. The protein and lipid contents were higher in seeds and ash content was higher in leaves. In seeds, total 18 essential and non-essential amino acids were present; total 17 amino acids were found in leaves, cysteine was absent. The essential and n...
Poster
The effect of Immunostimulatory properties and their remedial measures have been studied in catfish Clarias batrachus (magur) fry (352.4 ± 7.91 mg). Fish fry were acclimatized for 10 days and then fed with four different diets: 0.5% seeds (D1), 0.5% leaves (D2), 0.25% leaves (D3) of Achyranthes aspera and control (D4). After 60 days, all groups wer...
Article
Full-text available
The impact of water temperature on the physiology of Channa punctata (Bloch, 1793) was evaluated in the present study. Fish were acclimated at 25 ± 1 °C and then exposed at six different temperatures: 10, 15, 20, 25, 30, and 35 °C. C. punctata exposed at 10, 15, and 20 °C showed 30, 21, and 11% reduced food consumption, respectively compared to 25...
Article
Full-text available
The effect of dietary supplementation of leaves and seeds of Achyranthes aspera on rohu, Labeo rohita fingerlings was evaluated. Three experimental diets containing 0.25 (D1) and 0.5% (D2) leaves and 0.5% (D3) seeds of A. aspera and control diet (D4) without plant ingredients were formulated. After 45 days of feeding, rohu were immunized with 20% c...
Article
Full-text available
The impact of plant-based diets on the digestive physiology of rohu Labeo rohita fingerlings (10.66 ± 0.53 g) was evaluated. A diet with all protein supplied by fishmeal was included as a control (F). Four test diets containing 300 g/kg protein were formulated using the following plant ingredients and fishmeal in a 1:1 blend: almond oil-cake Termin...
Article
Full-text available
Purpose: Light intensity is one of the important environmental factors that have strong influence on larviculture. Culture of fish at high intensity of light generates harmful radicals in the body that can compromise their health and production. The present study aims to evaluate the effect of various light intensities on the physiology of rohu Lab...
Article
The culture technique of greater duckweed Spirodela polyrhiza (L. Schleiden) was standardized in outdoor tanks using three different manures: manure 1 - cattle manure, poultry droppings and mustard oil cake, manure 2 - urea, potash and triple superphosphate and manure 3 - cattle manure, urea, potash and triple superphosphate. Significantly (p < .05...
Article
Full-text available
The stress ameliorating effect of dietary supplementation of vitamin C, vitamin E, and tryptophan on rohu Labeo rohita fry was evaluated. Rohu fry (1.1 ± 0.03 g) were cultured under five different feeding regimes: enriched with 0.08% vitamin C (D1), 0.02% vitamin E (D2), 1.42% tryptophan (D3), a combination of these three ingredients at similar dos...
Article
Full-text available
The immunostimulatory and disease-resistance properties of Achyranthes aspera were evaluated in rohu (Labeo rohita) challenged with Aeromonas hydrophila. Experimental diets were enriched with leaves at 0.25% (D1) and 0.5% (D2) and seeds at 0.5% (D3); the control diet (D4) was without any enrichment. Rohu (2.02 ± 0.23 g) were cultured for 75 days an...
Article
The immunostimulatory properties of seeds and leaves of Achyranthes aspera (Linn.) was evaluated in rohu Labeo rohita in the pond conditions. Rohu fry (1.9 ± 0.08 g) were introduced in nine hapas (25 hapa−1) set inside a pond. Two test diets enriched with 0.5% seeds (D1) and leaves (D2) of A. aspera and control diet (D3) were fed for 60 days. Then...
Article
Full-text available
The immunostimulatory and disease resistance properties of herb Achyranthes aspera L. (Amaranthaceae) were evaluated in rohu Labeo rohita in pond. Rohu fry (1.9 AE 0.08 g) were cultured in hapas (25 hapa À1) set inside a pond and were fed with two experimental diets containing 0.5% seeds (D1) and leaves (D2) of A. aspera and control diet (D3). Fish...
Poster
Abstract Fish and fishery products represent a very valuable source of protein and essential micronutrients for good health. Diseases and various parasitic infections are responsible for major losses to aquaculture industry. Immunostimulation is one of the useful tools to prevent infectious diseases. The use of herbal compounds as immunostimulants...
Article
Full-text available
The development of UV-B protective mechanisms in aquacultural species is essential for the sustainable production of healthy aqua crop. Freshwater carp Catla catla larvae (13.5 ± 1.12 mg) were fed with a diet containing 0.5% vitamin C (D1) and a control diet (D2) for 40 days. Each group was exposed to two doses of UV-B irradiation: 360 (5 min, D15...
Article
Development of UV-B protective mechanism in aquacultural species is essential for sustainable production of healthy aqua crop. Freshwater carp Catla catla larvae (13.5 ± 1.12 mg) were fed with diet containing 0.5% vitamin C (D1) and control diet (D2) for 40 days. Each group was exposed at two doses of UV-B irradiation: 360 (5 min, D15min, D25min) a...
Article
The serine protease trypsin was isolated and purified from the digestive system of carp Labeo rohita rohu by ammonium sulphate precipitation, ion exchange, and affinity chromatography. The purified enzyme showed high activity between pH 7.0 and 9.0. The activity was maximum at 40°C. Incubation of the purified enzyme with CaCl2 (2 mM) stabilized the...
Article
Full-text available
The surface floating duckweed Lemna minor (Lemnaceae) is a potential ingredient to replace the application of fish-meal in the aqua-feed. The culture technique of the duckweed was standardized in outdoor tanks and then applied in the pond. Three consecutive experiments were conducted in tanks (1.2 × 0.35 × 0.3 m). In experiment 1, four different ma...
Article
Full-text available
Temperature plays significant role in the oxidation of ammonia in filtration units of recirculating aquaculture system. The impact of temperature on the abundance of ammonia oxidizing archaea and bacteria, and the expression of ammonia oxidizing gene (amoA) at specific temperature was evaluated. The broken earthen pot pieces used as filter bed mate...
Article
Full-text available
The role of ammonia-oxidizing bacteria and archaea was evaluated using amoA gene in a freshwater recirculating system. Broken earthen pot pieces (BEP) were used as filter bed material. Five archaeal and four bacterial operational taxonomic units were retrieved from amoA genes. Shannon-Weiner and Simpson indices were higher in archaeal amoA sequence...
Article
Fish face the challenge of diurnal and seasonal fluctuations of temperature in culture and it is essential to understand their physiological responses to thermal stress. Indian major carp Catla catla, catla (7.52 ± 0.22 g) were acclimated at 28 ± 1 °C for 21 days and then exposed at six different temperatures: 10, 15, 20, 25, 28 and 35 °C. Blood an...
Article
Full-text available
Nutrition plays significant role in the survival, growth and physiology of fish. Snow trout Schizothorax richardsonii larvae were cultured under three feeding regimes: control diet (diet D1) and test diets, supplemented with vitamin C (800 mg/kg diet, diet D2) and Achyranthes aspera seeds (5 g/kg diet, diet D3). There was no significant (P > 0.05)...
Article
Full-text available
Aquaculture, as a promising food industry, is expected to meet the demand for quality food from the increasing human population. As the diet is critical for feeding farm fish, such a faster growth in the industry is destined to create stress in the fishmeal market to supply diets to the tune. In this context, here, we studied the protein content of...
Article
Full-text available
Catla catla catla (2.28 ± 0.1 g) were exposed to six different levels of dissolved oxygen: 1 (DO-1), 3 (DO-3), 5 (DO-5), 7 (DO-7), 9 (DO-9) and 11 (DO-11) mg/L. DO-5 served as control. In DO-1 and DO-3, the number of red blood cells (RBC), lysozyme, respiratory burst activity and nitric oxide synthase were significantly (p < 0.05) lower compared to...
Article
Full-text available
Digestive enzyme profile is a good indicator of the nutritional and health status of the fish. The present investigation aims to evaluate the effect of exogenous bath treatment of hormones, cortisol and triiodothyronine, on the digestive enzyme activities and growth of carp Catla catla (Ham.) during ontogenic development. Catla larvae (4 days old)...
Article
The present investigation was carried out to study gut content and accompanying digestive enzyme profiles of the three carp species, golden mahseer Tor putitora, snow trout Schizothorax richardsonii and hill trout Barilius barila. The fishes were captured from the River Ladhiya, Champawat district, Uttarakhand, northern India. The digestive tract l...
Article
The damage-associated molecular patterns (DAMPs) released from the damaged tissue/cells are recently reported as endogenous ligands to activate toll-like receptors (TLRs) and nucleotide binding and oligomerization domain (NOD) receptors signaling pathways. In the aquatic environment, reduction in dissolved oxygen (DO) concentration causes hypoxic s...
Article
Full-text available
Toll-like receptors (TLRs) and nucleotide binding and oligomerization domain (NOD) receptors are pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs) and play crucial role in innate immunity. In addition to PAMPs, PRRs recognize endogenous molecules released from damaged tissue or dead cells [damage-ass...
Article
Trypsin was purified 35.64-fold with 4.97% recovery from the viscera of carp Cirrhinus mrigala (mrigal) by ammonium sulfate precipitation, ion exchange and affinity chromatography. The purified enzyme was active at a wide range of pH (7.0–9.2) and temperature (10–50 °C). The purified enzyme exhibited high thermal stability up to 50 °C for 1 h. The...
Article
Full-text available
UV-B radiation is a potential stressor to the aquacultural species. Catla catla, catla larvae (1.08±0.065g) were exposed to different doses of UV-B radiation, 0 (control), 504, 1008, 1512 and 2016mJ/cm(2) at a mean radiant energy of 80μW/cm(2) for 21days. The dose of UV-B radiation was selected on the basis of the field study conducted in Lake Nain...
Article
Full-text available
The antioxidant and growth stimulating properties of seeds of Achyranthes aspera were evaluated on UV-B irradiated Catla catla (catla) larvae. Catla larvae (initial weight: 1.2 ± 0.01 mg) were fed with four different diets--D1, D2 and D3 containing 0.1, 0.25 and 0.5% seeds of A. aspera and D4, control diet for 35 days. The larvae were then exposed...
Article
Full-text available
Abstract The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, followed by the capacity...
Article
Full-text available
Catla catla (Family: Cyprinidae) were exposed to 10, 15, 20, 25, 30, 33 and 35 �C following 28 �C acclimation temperature. Temperature change rate was 2 �C/day. Mortality rate of fish was recorded. In 10 �C temperature group, 17 and 65 % mortality was recorded at 14 and 10 �C, respectively. Significantly (P\0.05) higher mortality was recorded in fi...
Article
Full-text available
The present investigation aimed to evaluate the effect of feeding regimes composed of various combinations of live food (LF), artificial food (AF) and mixed food (MF: LF and AF 50% each) on the digestive physiology of first feeding Catla catla (catla) larvae. Larvae (0.92 ± 0.21 mg) were cultured for 30 days under six different feeding regimes of...
Article
The effect of temperature on the food consumption rate and the digestive enzyme activities of Clarias batrachus (80.60 +/- 5.34g) were evaluated. Fish were exposed to six different temperatures of 10, 15, 20, 25, 30 and 35 degrees C following an acclimation temperature of 25 degrees C. The rate of temperature change was 2 degrees Cday(-1). Highest...
Article
Full-text available
___________________________________________________________________________________The present study aimed to evaluate the inhibitory effect of embelin against UVB induced oxidative stress using in vivo (Catla catla) fish model. Larve [Catla catla (1.2+0.01 mg)] were fed with three types of diets control diet (Group I & Group II with and without UV...
Article
Full-text available
Background. Ultraviolet (UV-B) radiation affects the immune system of fish. Dietary supplementation of natural immunostimulants may enhance the immunity of fish. The presently reported investigation evaluates the UV-B protective property of Achyranthes aspera seed in larvae of rohu, Labeo rohita (Hamilton, 1822). Materials and methods. Larvae (1.19...
Article
Full-text available
Evaluation of functional mechanism of immunostimulant in fish is essential for realizing their therapeutic potential. The impact of dietary supplementation of seeds of Achyranthes aspera on the immune system of Catla catla was studied. Catla (37.73 ± 6.7 g) were fed with test diets containing 0.5 (D1) and 1.0% (D2) seeds of Achyranthes aspera and c...
Article
Full-text available
Effect of harmful UV-B radiation and their remedial measures have been studied in Labeo rohita (rohu) larvae. Larvae were fed with four different diets: D1 and D2 contained 0.1 and 0.5 % Achyranthes aspera seeds, respectively; D3 contained 800 mg vitamin C/kg diet, and D4 contained control diet. After 50 days, all groups were exposed to UV-B radiat...
Article
Full-text available
Temperature plays significant roles in the life of poikilothermic aquatic animal fish. The physiological responses of Clarias batrachus (Linnaeus 1758), magur (80.60±5.34 g) at different temperatures were observed. Fish were acclimated at 25 °C for 21 days and were exposed at 10, 15, 20, 25, 30 and 35 °C temperatures. Blood and muscle samples were...
Article
Full-text available
Abstract Larval rohu Labeo rohita were fed four different diets: three of the diets contained Achyranthes aspera (prickly chaff-flower) seeds at 0.10% (D1), 0.25% (D2), or 0.50% (D3); the fourth diet was a control diet (D4; no A. aspera supplementation). After 70 d, the rohu were injected intraperitoneally with live Aeromonas hydrophila. Mortality...
Article
Full-text available
Trypsin was purified from the digestive system of carp Catla catla (Hamilton) by ammonium sulfate fractionation, diethylaminoethyl-cellulose column chromatography, and Benzamidine Sepharose 4 fast flow column affinity chromatography. Trypsin was purified 26.2-fold with an 11.1% yield. The purified enzyme was active between pH 7.0 and 9.8, and maxim...