Richard J Miron

Richard J Miron
  • DDS, BMSC, MSc, PhD, Dr. med. dent.
  • Nova Southeastern University

About

373
Publications
357,980
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
13,230
Citations
Current institution
Nova Southeastern University
Additional affiliations
September 2011 - August 2015
Université Laval
Position
  • Research Associate
September 2012 - August 2015
Université Laval
Position
  • PostDoc Position
December 2011 - present
Wuhan University
Description
  • Post-doctoral Research Assistant
Education
September 2011 - May 2015
Université Laval
Field of study
  • Dentistry
November 2009 - August 2012
University of Bern
Field of study
  • Periodontology and Cell Biology
August 2007 - August 2009
Western University
Field of study
  • Cell Biology

Publications

Publications (373)
Article
Full-text available
Since the discovery of osteoinduction in the early 20th century, innovative biomaterials with osteoinductive potential have emerged. Over the last 50 years, however, our ability to describe biological phenomena accurately has been improved dramatically by advancements in cell and molecular biology. The aim of this review is to divide the osteoinduc...
Article
Full-text available
Objectives The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF). Materials and m...
Article
Full-text available
Aims: This review article aims to: 1) provide the biological background necessary to understand the rational for the use of EMD for periodontal regeneration, 2) present animal and human histological evidence of periodontal regeneration following EMD application, 3) provide clinically relevant indications for the use of EMD and 4) discuss future av...
Article
Full-text available
The health of peri-implant soft tissues is one of the most important aspects of osseointegration necessary for the long-term survival of dental implants. To review the process of soft tissue healing around osseointegrated implants and discuss the maintenance requirements as well as the possible short-comings of peri-implant soft tissue integration....
Article
Type 2 diabetes mellitus (T2DM) affects millions globally and is strongly associated with oral health issues, particularly periodontitis. The bidirectional relationship between T2DM and periodontitis is well established, with poorly managed T2DM increasing the risk of inflammation, tissue damage, and dental implant failure. Advances in treatment, s...
Article
This clinical narrative review aimed to comprehensively evaluate the effects of platelet-rich fibrin (PRF) on clinically-related and patient-reported outcomes in palatal wound healing following autologous palatal graft harvesting. A systematic and thorough electronic search was performed in the PubMed, Scopus, and Web of Science databases up to Nov...
Article
Mesenchymal stem cells are highly regarded for their potential in tissue repair and regenerative medicine due to their multipotency and self-renewal abilities. Recently, mesenchymal stem cells have been redefined as “medical signaling cells,” with their primary biological effects mediated through exosome secretion. These exosomes, which contain lip...
Article
Full-text available
This systematic review aimed to evaluate all available evidence across all fields of medicine regarding the comparative effectiveness of platelet‐rich plasma (PRP) versus injectable platelet‐rich fibrin (i‐PRF). A comprehensive search was conducted in PubMed, Scopus, and Web of Science up to September 30, 2024. Following a thorough screening proces...
Article
Aim: The aim of this comprehensive review was to evaluate comparative studies on horizontal and fixed-angle centrifugation methods for preparing platelet-rich fibrin (PRF). Furthermore, additional studies utilizing horizontal PRF (H-PRF) were systematically investigated. This overview review articles offers deeper insights into the advantages of H-...
Article
Full-text available
In recognizing the critical role of vitamin D in bone metabolism and osseointegration, research aims to identify whether preoperative vitamin D deficiency serves as a risk factor for early implant failure. By analyzing patient outcomes and their serum vitamin D levels, studies seek to establish evidence‐based recommendations for vitamin D assessmen...
Article
Objectives: Exosomes are essential mediators/communicators of tissue repair via well-established mechanisms of action that include stimulatory effects on angiogenesis and cellular proliferation, differentiation, and matrix biosynthesis. This pioneering clinical study aimed to evaluate the effectiveness of exosomes specifically designed for periodon...
Preprint
Full-text available
Objectives: Exosomes are essential mediators/communicators of tissue repair via well-established mechanisms of action that include stimulatory effects on angiogenesis and cellular proliferation, differentiation, and matrix biosynthesis. This pioneering clinical study aimed to evaluate the effectiveness of exosomes specifically designed for periodon...
Article
Introduction Platelet‐rich fibrin (PRF) is being increasingly utilized in surgical procedures due to various improvements in clinical outcomes. More recently, a heating process to denature albumin in the platelet poor plasma (PPP) layer has been shown to extend the resorption time of PRF from a typical 2‐week period to 4–6 months. Because of its >...
Article
Full-text available
To provide an overview of the use of autogenous platelet concentrates (APCs) in periodontal regeneration and to conduct a systematic review (SR) of the treatment outcomes of periodontal intrabony defects by using platelet‐rich fibrin (PRF) compared with other commonly utilized modalities. The eligibility criteria comprised randomized controlled tri...
Article
Full-text available
The objective of the study was to compare the treatment outcomes of periodontal furcation defects by using platelet‐rich fibrin (PRF) with other commonly utilized modalities. The eligibility criteria comprised randomized controlled trials (RCTs) comparing the clinical outcomes of PRF with those of other modalities for the treatment of furcation def...
Article
Objectives To reveal the force profiles recorded by haptic autonomous robotic force feedback during the transcrestal sinus floor elevation (TSFE) process, providing a reference for the surgery strategy during TSFE. Materials and methods A total of 42 maxillary sinus models with different angles of the sinus floor (30°, 40°, 50°, 60°, 70°, 80°, and...
Article
Full-text available
Objectives To assess the accuracy and effectiveness among operators with different levels of experience in a robot-assisted immediate implant surgery. Materials and methods The study included four participants who had received dental training at the same institution but have varying levels of clinical experience in implant dentistry, denoted as un...
Article
Full-text available
This narrative review summarizes current knowledge on the use of autologous platelet concentrates (APCs) in esthetic medicine, with the goal of providing clinicians with reliable information for clinical practice. APCs contain platelets that release various growth factors with potential applications in facial and dermatologic treatments. This revie...
Article
Full-text available
Currently, autologous platelet concentrates (APCs) are frequently used for soft‐ and hard‐tissue regeneration, not only within the oral cavity, but also extra‐orally including chronic wounds, burns, joints, dermatological conditions, among others. The benefits of APCs are largely influenced by the treatment strategy but also their preparation. This...
Article
Full-text available
Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with the ability to communicate with other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due to their ability to be utilized as therapeutic options for a wide array of diseases/conditio...
Article
Full-text available
Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with the ability to communicate with other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due to their ability to be utilized as therapeutic options for a wide array of various diseases....
Article
Full-text available
Horizontal platelet-rich fibrin (H-PRF) contains a variety of bioactive growth factors and cytokines that play a key role in the process of tissue healing and regeneration. The blood collection tubes used to produce Solid-PRF (plasmatrix (PM) tubes) have previously been shown to have a great impact on the morphology, strength and composition of the...
Article
Full-text available
Bone grafts are typically categorized into four categories: autografts, allografts, xenografts, and synthetic alloplasts. While it was originally thought that all bone grafts should be slowly resorbed and replaced with native bone over time, accumulating evidence has in fact suggested that the use of nonresorbable xenografts is favored for certain...
Article
Full-text available
Osteoporosis is a chronic multifactorial condition that affects the skeletal system, leading to the deterioration of bone microstructure and an increased risk of bone fracture. Platelet-derived biomaterials (PDBs), so-called platelet concentrates, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF), have shown potential for improving...
Article
Full-text available
The use of platelet‐rich fibrin (PRF) has seen widespread advantages over platelet‐rich plasma (PRP) in many fields of medicine. However, until 2014, PRF remained clinically available only in its solid clotted form. Modifications to centrifugation protocols and tube technology have led to the development of a liquid injectable version of PRF (i‐PRF...
Article
Full-text available
Platelet‐rich fibrin (PRF) has been characterized as a regenerative biomaterial that is fully resorbed within a typical 2–3 week period. Very recently, however, a novel heating process was shown to extend the working properties of PRP/PRF from a standard 2–3 week period toward a duration of 4–6 months. Numerous clinicians have now utilized this ext...
Article
Full-text available
Implant dentistry has evolved to the point that standard implant osseointegration is predictable. This is attributed in part to the advancements in material sciences that have led toward improvements in implant surface technology and characteristics. Nonetheless, there remain several cases where implant therapy fails (specifically at early time poi...
Chapter
Over 40 years have now passed since bone grafting materials were utilized for the regeneration of missing bone around teeth and dental implants. Today an ever-increasing number of bone substitute materials are available, each with various properties and characteristics. While bone grafts are typically classified into autografts, allografts, xenogra...
Article
Full-text available
The use of biomaterials in regenerative medicine has expanded to treat various disorders caused by trauma or disease in orthopedics and dentistry. However, the treatment of large and complex bone defects presents a challenge, leading to a pressing need for optimized biomaterials for bone repair. Recent advances in chemical sciences have enabled the...
Article
Full-text available
Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with a diameter in the range of 30–150 nm. Their use has gained great momentum recently due to their ability to be utilized as diagnostic tools with a vast array of therapeutic applications. Over 5000 publications are currently being published yearly on this...
Article
Full-text available
This systematic review (SR) aimed to evaluate the antimicrobial potential of different types of platelet‐rich fibrin (PRF) often used in regenerative treatments. An electronic search was performed in four databases and in Gray literature for articles published until January, 2023. The eligibility criteria comprised in vitro studies that evaluated t...
Article
Full-text available
Peri‐implantitis is a pathogenic inflammatory condition characterized by progressive bone loss and clinical inflammation that may compromise the stability of dental implants. Therapeutic modalities have been advocated to arrest the disorder and to establish peri‐implant health. Reconstructive therapy is indicated for bone defects exhibiting contain...
Article
Full-text available
Contemporary oral tissue engineering strategies involve recombinant human growth factor approaches to stimulate diverse cellular processes including cell differentiation, migration, recruitment, and proliferation at grafted areas. Recombinant human growth factor applications in oral hard and soft tissue regeneration have been progressively research...
Article
Full-text available
The use of platelet‐rich fibrin (PRF) has gained tremendous popularity in recent years owing to its ability to speed wound healing postsurgery. However, to date, many clinicians are unaware of methods designed to optimize the technology. This overview article will discuss the advancements and improvements made over the years aimed at maximizing cel...
Article
Full-text available
Implantable biomaterials are widely used in bone tissue engineering, but little is still known about how they initiate early immune recognition and the initial dynamics. Herein, the early immune recognition and subsequent osteoinduction of biphasic calcium phosphate (BCP) after implantation to the protein adsorption behavior is attributed. By liqui...
Article
Tissue engineering focuses on wound healing and tissue regeneration. Platelet-rich fibrin (PRF) is a fibrin matrix contain- ing cytokines, growth factors and cells that are gradually released into the wound over time. This study aimed to evaluate the effect of PRF membranes on wound repair and microbial control in infected wounds. Skin wounds were...
Article
Full-text available
Plain Language Summary After tooth loss, the alveolar bone (which supports the teeth) undergoes a natural process called bone remodeling, which can lead to significant decreases in bone height and thickness over time. Faced with the need to replace missing teeth, especially when it comes to dental implants, the lack of supporting tissues can compro...
Article
Full-text available
Partial extraction therapy (PET) is a set of surgical techniques that preserve a portion of the patient's own root structure to maintain blood supply derived from the periodontal ligament complex in order to maintain the periodontium and peri-implant tissues during restorative and implant therapy. PET includes the socket shield technique (SST), pro...
Article
Full-text available
Partial extraction therapy (PET) is a group of surgical techniques that preserves the periodontium and peri-implant tissues during restorative and implant therapy by conserving a portion of the patient's own root structure to maintain blood supply derived from the periodontal ligament complex. PET includes the socket shield technique (SST), proxima...
Article
Full-text available
Objectives The aim of the present study was to compare the barrier function during bacterial invasion and wound healing properties of 3 commonly used membranes including horizontal platelet-rich fibrin (H-PRF) against two commercially available resorbable collagen membranes. Materials and methods H-PRF membranes were prepared by collecting venous...
Article
Full-text available
Zirconium implants have gained popularity among clinicians due to their superior mechanical properties. However, zirconium implants usually perform less well in early osseointegration than titanium implants. And the degree of severity of the acute inflammation resulting from macrophage activation after implantation determines the result of the impl...
Preprint
Full-text available
Objectives: The effects of horizontal platelet-rich fibrin (H-PRF) bone block on the healing and immune response during sinus augmentation have not been fully investigated histologically at early time points. Materials and Methods: Eighteenth male New Zealand white rabbits underwent bilateral sinus augmentation and were divided into two groups: dep...
Article
Background: While suggested to be effective in tissue regeneration, the effects of horizontal platelet-rich fibrin (H-PRF) bone block in sinus augmentation have not been verified in an animal model. Methods: Twelve male New Zealand white rabbits that underwent sinus augmentation were divided into two groups: deproteinized bovine bone mineral (DB...
Article
Full-text available
Platelet-rich fibrin (PRF) has a potent anti-inflammatory activity but the components mediating this effect remain unknown. Blood lipids have anti-inflammatory properties. The question arises whether this is also true for the lipid fraction of PRF. To answer this question, lipid fractions of solid and liquid PRF were tested for their potential to l...
Article
Implants are widely used in medical applications and yet macrophage-mediated foreign body reactions caused by implants severely impact their therapeutic effects. Although the extensive use of multiple surface modifications has been introduced to provide some mitigation of fibrosis, little is known about how macrophages recognize the stiffness of th...
Article
Full-text available
Sepsis is a dysregulation of the immune response to pathogens and has high morbidity and mortality worldwide. However, the unclear mapping and course of dysregulated immune cells currently hinders the development of advanced therapeutic strategies to treat sepsis. Here, evidence is provided using single‐cell RNA sequencing from peripheral blood mon...
Article
Full-text available
Most available antiseptic solutions have strong antibacterial effects, but many also possess major cytotoxic effects on gingival fibroblasts, osteoblasts, osteoprogenitor cells, and/or epithelial cells. A novel VEGA Oral Care Recovery Kit (StellaLife) consisting of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of the Un...
Article
Full-text available
Dental implant failure cannot always be explained by clinical risk factors. Recent literature suggests that immune cells are pivotal players in the integration of biomaterials and have a co-relationship within a set of osteal macrophages known as "OsteoMacs." These cells have been known to polarize quickly between a M1 pro-infl ammatory and a M2 wo...
Article
Full-text available
Objectives Platelet-rich fibrin (PRF) in liquid form has shown advantages in tissue engineering including acting as injectable fillers and drug carriers. However, few studies have investigated the best relative centrifugal force (RCF) for preparing liquid PRF. The aim of the present study was to find out optimal centrifugation force for preparing l...
Article
Full-text available
Tissue engineering focuses on wound healing and tissue regeneration. Platelet-rich fibrin (PRF) is a fibrin matrix containing cytokines, growth factors and cells that are gradually released into the wound over time. This study aimed to evaluate the effect of PRF membranes on wound repair and microbial control in infected wounds. Skin wounds were pe...
Article
Full-text available
Conventional approaches to full-arch implant dentistry require a verified master model created by luting together impression jigs. This process involves numerous steps and is sometimes prone to errors that require subsequent correction. A novel approach involving an extraoral scanning technique using an Imetric 4D Imaging system demonstrates an alt...
Article
Full-text available
Lasers are increasingly utilized in the non-surgical treatment of periodontal disease. The aim of the present systematic review with meta-analysis was to investigate the combination use of Nd:YAG and Er:YAG laser therapy in the treatment of severe periodontitis. A systematic review of the literature including searches in PubMed/Medline, Cochrane Li...
Article
Full-text available
Objective To evaluate the effect of resting and compression time after centrifugation on the physical properties of platelet rich fibrin (PRF) membranes, and to provide optimal guidance regarding the clinical preparation of PRF. Materials and methods A total of 12 volunteers enrolled in this study divided into 2 groups equally. For each volunteer,...
Article
Full-text available
Purpose: The aim of the present systematic review with meta-analysis was to investigate the clinical effectiveness of EMD (enamel matrix derivative) using a minimally invasive surgical technique (MIST) or flapless approach for the treatment of severe periodontal probing depths. Materials and methods: A systematic review of the literature includi...
Article
Full-text available
The preparation of platelet-rich fibrin (PRF) requires blood centrifugation to separate the yellow plasma from the red erythrocyte fraction. PRF membranes prepared from coagulated yellow plasma are then transferred to the defect sites to support tissue regeneration. During natural wound healing, however, it is the unfractionated blood clot (UBC) th...
Article
The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet concentrates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were distributed into three groups: Control, L-PRF,...
Article
Full-text available
With the rapid development of nanotechnology, nanoparticles (NPs) are widely used in all fields of life. Nowadays, NPs have shown extraordinary antimicrobial activities and become one of the most popular strategies to combat antibiotic resistance. Whether they are equally effective in combating bacterial persistence, another important reason leadin...
Article
Full-text available
Liquid platelet-rich fibrin (PRF) is produced by fractionation of blood without additives that initiate coagulation. Even though liquid PRF is frequently utilized as a natural source of fibrinogen to prepare sticky bone, the concentration of fibrinogen and the overall amount of “clottable PRF” components have not been evaluated. To this aim, we pre...
Article
Stem and progenitor cells play important roles in the development and maintenance of teeth and bone. Surface markers expressed in bone marrow–derived mesenchymal stem cells are also expressed in dental tissue–derived stem cells. Mouse skeletal stem cells (mSSCs, CD45 ⁻ Ter119 ⁻ Tie2 ⁻ CD51 ⁺ Thy ⁻ 6C3 ⁻ CD105 ⁻ CD200 ⁺ ) and human skeletal stem cel...
Article
Full-text available
Objectives We previously showed that accelerated degradation of collagen membranes (CMs) in diabetic rats is associated with increased infiltration of macrophages and blood vessels. Since pre-implantation immersion of CMs in cross-linked high molecular weight hyaluronic acid (CLHA) delays membrane degradation, we evaluated here its effect on the nu...
Article
Full-text available
Objectives Platelet-rich fibrin (PRF) has been utilized in regenerative medicine as a concentration of autologous platelets and growth factors that stimulates tissue regeneration. More recently, liquid-PRF (also called injectable-PRF; i-PRF) has been brought to market utilizing PET plastic tubes. Due to new advances made in tube technology, the fir...
Article
Full-text available
Objectives: To investigate physicochemical properties, dentin bonding, cytotoxicity, and in vivo pulp response of experimental self-adhesive composites tailored to direct pulp capping. Materials and methods: Experimental composites were prepared with beta-tricalcium phosphate and hydroxyapatite nanoparticles adsorbed with simvastatin and glutath...
Article
Platelet-rich fibrin (PRF) has been utilized as a substitute for resorbable membranes during guided bone regeneration (GBR) therapy as it is a more bioactive biomaterial with living cells and growth factors than resorbable membranes. Nevertheless, PRF poses obvious disadvantages in its mechanical strength since its rapid degradability has been show...
Article
Full-text available
Purpose: To histologically compare the effects of platelet-rich fibrin (PRF) produced using different protocols on periodontal wound healing/regeneration in periodontal defects in dogs. Materials and methods: Dehiscence-type gingival recession and two-wall intrabony defects were created bilaterally in the maxillary canines and mandibular premola...
Article
Full-text available
Chronic inflammation is a pathological process where cells of the mesenchymal lineage become a major source of inflammatory mediators. Platelet-rich fibrin (PRF) has been shown to possess potent anti-inflammatory activity in macrophages, but its impact on mesenchymal cells has not been investigated. The aim of this study was, therefore, to expose m...
Article
Full-text available
Objectives The aim of this study was to evaluate the in vitro effect of enamel matrix derivative (EMD) and hyaluronic acid (HA) and their synergistic combination on lipopolysaccharides (LPS)-induced inflammation in human keratinocytes and osteoblasts.Material and methodsCells were challenged with LPS (1 μg/ml) and cultured in the following treatmen...
Article
Purpose: To investigate the effect of platelet-rich fibrin on bone formation by investigating its use in guided bone regeneration, sinus elevation and implant therapy. Materials and methods: This systematic review and meta-analysis were conducted and reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analys...
Article
Alveolar bone defects caused by tooth loss often lead to challenges in implant dentistry, with a need for development of optimal bone biomaterials to predictably rebuild these tissues. To address this problem, we fabricated a novel bone block using platelet-rich fibrin (PRF) and Deproteinized Bovine Bone Mineral (DBBM), and characterized their mech...
Article
Full-text available
Solid platelet-rich fibrin (PRF), consisting of coagulated plasma from fractionated blood, has been proposed to be a suitable carrier for recombinant bone morphogenetic protein 2 (BMP2) to target mesenchymal cells during bone regeneration. However, whether solid PRF can increase the expression of BMPs in mesenchymal cells remains unknown. Proteomic...
Article
Full-text available
Biomineralization is a chemical reaction that occurs in organisms in which collagen initiates and guides the growth and crystallization of matched apatite minerals. However, there is little known about the demand pattern for calcium salts and collagen needed by biomineralization. In this study, natural bone biomineralization is analyzed, and a nove...
Article
Purpose: To investigate the use of platelet-rich fibrin for alveolar ridge preservation compared to natural healing, bone graft material and platelet-rich fibrin in combination with bone graft material. Materials and methods: The present systematic review was conducted and reported according to the Preferred Reporting Items for Systematic Review...
Article
Full-text available
Objectives This study aims to compare the treatment outcomes of periodontal intrabony defects by using platelet-rich fibrin (PRF) with other commonly utilized modalities. Materials and methods The eligibility criteria comprised randomized controlled trials (RCTs) comparing the clinical outcomes of PRF with that of other modalities. Studies were cl...
Preprint
Full-text available
Accelerated degradation of collagen membranes (CMs) in diabetic rats is associated with increased infiltration of macrophages and blood vessels. Since pre-implantation immersion of CMs in cross-linked high molecular weight hyaluronic acid (CLHA) delays membrane degradation, we evaluated its effect on the number of macrophages and endothelial cells...
Article
Full-text available
Macrophage activation and osteoclastogenesis are hallmarks of inflammatory osteolysis and may be targeted by the local application of liquid platelet-rich fibrin (PRF). Liquid PRF is produced by a hard spin of blood in the absence of clot activators and anticoagulants, thereby generating an upper platelet-poor plasma (PPP) layer, a cell-rich buffy...
Article
Objective To investigate the efficacy of platelet-rich fibrin (PRF) for the socket healing after removal of mandibular third molars when compared to natural wound healing. Methods This systematic review and meta-analysis were conducted according to PRISMA guidelines. The eligibility criteria comprised randomized controlled trials (RCTs) comparing...
Article
Full-text available
T cell immunotherapy holds significant challenges in solid tumors, mainly due to the T cells’ low activation and the decreased synthesis–release of therapeutic proteins, including perforin and granzyme B, which are present in lysosomes. In this study, a lysosome‐targeting nanoparticle (LYS‐NP) is developed by way of a mineralized metal–organic fram...
Article
Full-text available
Objectives: To describe the step-by-step procedure of a novel surgical technique consisting of a combination of the laterally closed tunnel (LCT) and the modified coronally advanced tunnel (MCAT) (ie, LCT/MCAT), designed to treat multiple mandibular adjacent gingival recessions (MAGR) and to present the clinical outcomes obtained in 11 consecutive...
Article
Full-text available
Background Platelet-rich fibrin (PRF) has been widely utilized in modern medicine and dentistry owing to its ability to rapidly stimulate neoangiogenesis, leading to faster tissue regeneration. While improvements over traditional platelet rich plasma therapies (which use chemical additives such as bovine thrombin and calcium chloride) have been obs...
Chapter
Full-text available
Platelet concentrates were derived more than 20 years ago follow-ing the discovery that platelets themselves act as key regulators during the wound healing process. Initial attempts were first made to concentrate these cells using anticoagulants and a centrifugation device; the resulting biomaterial was called platelet-rich plasma (PRP). Shortly th...
Chapter
Full-text available
Much can be discussed with respect to the biology of PRF and its ability to impact tissue regeneration. During the natural wound healing process, vascularization of tissues plays a pivotal role, facilitating the invasion of incoming cells, growth factors (GFs), cytokines, and other regenerative factors. The main aim of platelet concentrates discove...
Chapter
Full-text available
The first two chapters of this textbook discussed the history of platelet concentrates and their commercial and biologic evolution. The aim of this chapter is to discuss centrifugation parameters that may best optimize the production of PRF. A great deal of work has been conducted at both the preclinical and clinical level to maxi-mize the regenera...
Chapter
Full-text available
This chapter is designed with the sole purpose of providing clinicians a better means to understand centrifugation parameters that will permit the optimization of their centrifugation device irrespective of the centrifugation system and trade names associated. A very clear understanding of the calculations needed to convert revolutions per minute (...
Chapter
Full-text available
This chapter is a relatively short one designed to simplify proto-cols for the production of PRF. In general, only three protocols are necessary for PRF therapy. The first is the standard PRF membrane in which ideally a higher platelet and leukocyte yield is harvested with an even distribution of cells within the upper 4- to 5-mL PRF layers. This i...
Chapter
Full-text available
Due to the need to rapidly begin centrifugation following blood draw, phlebotomy becomes an essential component for the production of PRF. Because no anticoagulants are utilized, centrifugation typically must be carried out within 60 to 90 seconds following blood draw, as reviewed in chapter 2. Therefore, office staff must be well equipped and prep...
Chapter
Full-text available
It is hard to imagine that the effect of centrifugation tubes may in fact be more relevant for the production of PRF than the actual centrifugation device. Tubes, which are generally purchased and range in price from $0.85 to $1.55 each, have a massive implication in the final outcomes of both solid-PRF and liquid-PRF. Simply, more hydrophilic tube...
Chapter
Full-text available
This chapter is dedicated to providing an overview of future clinical chapters on PRF with an accompanying video highlighting the widespread uses of PRF in regenerative dentistry. Over two decades have now passed since PRF was first utilized in clinical practice, and its use has exponentially increased over the past 5 years, with over 250 articles...
Chapter
Full-text available
This first clinical chapter focuses on the use of PRF for root cover-age procedures in comparison to other treatment modalities. In total, 17 RCTs are presented comparing the use of coronally advanced flap (CAF) with and without PRF versus connective tissue graft (CTG), enamel matrix derivative (EMD; Emdogain), and amniotic membrane. Reports on the...
Chapter
Full-text available
One of the main reported drawbacks of PRF use is its rather fast resorption properties characterized within a 2-to 3-week time frame. However, recent studies have demonstrated that by heating a liquid platelet-poor plasma (PPP) layer, the resorption properties of heated albumin (albumin gel) can be significantly extended from 2 weeks to more than 4...
Chapter
Full-text available
This chapter provides an overview of the armamentarium required to adequately introduce PRF into clinical practice. A variety of specialized instruments and equipment is needed in order to perform phlebotomy and be able to process PRF after centrifuga-tion. Instruments required include a PRF centrifugation system, a PRF box, a variety of hand instr...
Chapter
Full-text available
This chapter highlights the fabrication of various PRF modalities, including PRF membranes, PRF plugs, “sticky bone,” e-PRF membranes, liquid i-PRF, and liquid C-PRF, as well as the production of newer protocols utilized to make Bio-Filler, Bio-Graft, and Bio-Bone in custom trays. A step-by-step protocol is provided for each harvest-ing technique,...
Chapter
Full-text available
PRF has been successfully utilized for the management of soft/periodontal tissues as reviewed in previous chapters. This chapter highlights its use for bone regeneration in extraction site manage-ment. Specific to socket grafting, PRF has been investigated in over 20 clinical trials when utilized either alone or in combination with a bone grafting...

Questions

Question (1)
Question
I’m aware that a higher risk is observed in patients receiving bisphosphonates by IV (Gen Dent. 2010 Nov-Dec;58(6):484-92), but am curious to know specifically if recent studies have demonstrated a relationship between oral bisphosphonates, implants and osteonecrosis of the jaw. Is there a risk/relationship? And should implant patients taking oral BPs be informed of this possible additional risk?

Network

Cited By