Ricardo de Oliveira Orsi

São Paulo State University | Unesp · Department of Animal Production

A própolis é um produto apícola cuja produção pode ser uma importante fonte de renda para apicultores, estando seu sucesso atrelado a um conjunto de fatores. O objetivo deste trabalho foi avaliar o desempenho de diferentes tipos de coletores na produção e qualidade da própolis produzida por abelhas Apis mellifera L. africanizadas. Foram utilizadas...

The increase in the world population results in a greater demand for food, intensifying the dependence on pesticides use. Glyphosate, a pesticide widely used in crops, can contaminate resources collected by bees. In this context, this study aimed to analyze the transcriptome of the head of Africanized Apis mellifera bees during the foraging phase t...

Taking into consideration that bees can be contaminated by pesticides through the ingestion of contaminated floral resources, we can utilize genetic techniques to assess effects that are scarcely observed in behavioral studies. This study aimed to investigate the genetic effects of ingesting lethal and sublethal doses of the insecticide fipronil in...

Taking into consideration that bees can be contaminated by pesticides through the ingestion of contaminated floral resources, we can utilize genetic techniques to assess effects that are scarcely observed in behavioral studies. This study aimed to investigate the genetic effects of ingesting lethal and sublethal doses of the insecticide fipronil in...

Bees are important pollinators for ecosystems and agriculture, but their populations have suffered a decline that may be associated with several factors, including habitat loss, climate change, increased vulnerability to diseases and parasites, and use of pesticides. Neonicotinoids are nicotinic cholinergic receptor agonists and lethal and subletha...

Bees are important pollinators for ecosystems and agriculture; however, populations have suffered a decline that may be associated with several factors, including habitat loss, climate change, increased vulnerability to diseases and parasites and use of pesticides. The extensive use of neonicotinoids, including imidacloprid, as agricultural pestici...

Africanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the last 5 years with a total of 303 deaths. To treat such massive attacks, Brazilian researchers deve...

The identification of biogenic amines and some precursor amino acids and the adulteration through stable isotopes was carried out in 114 honey from different geographic regions in Brazil (states of São Paulo (SP) and Santa Catarina (SC)) as support for evaluating quality control and food safety. Serotonin was detected in all samples, while melatoni...

We analysed whether the source of zinc (inorganic and organic) influences the morphology of the hypopharyngeal gland (HPG) and the relative expression of major royal jelly protein (MRJP) genes in nursing bees at 6 days of age. For this, 15 colonies of Apis mellifera bees were subjected to the following treatments: Zn0 (control), zinc methionine (50...

Migratory beekeeping can harm the bee colonies if not executed properly. Here, colonies of Apis mellifera were transported (for one or two hours) or not, following proper technical standards. To analyze gene expression (defensin-1, abaecin, and HSP70), forager bees were collected immediately, 24, and 72 hours after transportation. Bee mortality and...

We analyzed the changes in the gene expression of forage honey bees (Apis mellifera) exposed to fipronil field-relevant dose (2.5 ppb/bee) by 1 or 4 h. A reduction in the expression of nine genes was observed after 4 h of exposure, five of which are related to the digestive system: endoglucanase E-4, MSS11 transcription activator, inositol monophos...

The objective of the study was to evaluate the effect of propolis on the digestibility of minerals in the diet of blue-fronted amazon ( Amazona aestiva ). For this, 10 adult birds (5 males and 5 females) were separated into two groups (Control and Propolis 0.5% m/m). The apparent digestibility coefficient (ADC) of the following minerals was analyze...

Honey is a natural product with beneficial properties to health and has different characteristics depending on the region of production and collection, flowering, and climate. The presence of precursor amino acids of- and biogenic amines can be important in metabolomic studies of differentiation and quality of honey. We analyzed 65 honeys from 11 d...

Background Bees are the most important group of pollinators worldwide and their populations are declining. In natural conditions, Apis mellifera depends exclusively on food from the field to meet its physiological demands. In the period of scarcity, available resources are insufficient and artificial supplementation becomes essential for maintainin...

For honey production, beekeepers add one or more supers to the hives to allow honeybees to store their products. However, the increase in hive space can affect the social and health organization in the colony, promoting stress. This study assessed the management of honey production, physicochemical honey properties, population development, and fora...

We investigated the effects of organic and inorganic zinc (Zn) supplementation on the morphology of mandibular glands in Apis mellifera L.. We randomly assigned 28 beehives to seven treatment groups (four beehives in each group) as follows: control (no Zn) and Zn organic or inorganic supplementation (25, 50, and 75 ppm organic or inorganic Zn, resp...

This study aimed to evaluate the quality of royal jelly produced by honeybees Apis mellifera supplemented with different concentrations of inorganic zinc (zinc sulfate monohydrate—0, 25, 50, and 75 ppm). Two-dimensional electrophoresis for the fractionation of royal jelly proteins was performed, and the zinc level was quantified by the flame atomic...

Inadequate quantity and quality of proteins in honey bee diet can cause weakening of their colonies and damage their resistance to agrochemical contamination, such as fipronil, which is highly toxic to bees. Thus, we tested the hypothesis if protein supplementation would improve longevity and locomotion of honeybees exposed to fipronil pesticide. C...

In this study, we analyzed Apis mellifera royal jelly proteome alterations induced by supplementation with different concentrations of dietary organic zinc (0, 25, 50, and 75 ppm). Proteins extracted from royal jelly were separated by electrophoresis and isoelectric focusing, and zinc-containing protein spots were identified by tandem mass spectrom...

Fish were fed diets containing dehydrated ginger powder (GgP) to determine its effects on growth, immune response, antioxidant capacity and resistance to Aeromonas hydrophila infection. 540 Nile tilapia were distributed in 36 250 L aquaria and fed six isonitrogenous and isoenergic diets supplemented with six graded levels of GpP: 0, 2.5, 5, 7.5, 10...

This study assessed the effects of different protein levels on the development of the mandibular gland (MG) in six-day-old worker bees (Apis mellifera) during the dearth period. Twenty colonies were randomly assigned to one of four experimental diets containing 0%, 23%, 25%, or 27% crude protein (CP), with five replicates each. The experimental die...

In this study the effects of inorganic iron (Fe) supplementation on the morphology of mandibular glands in Apis mellifera were investigated. We randomly assigned 12 beehives to four treatment groups (with three hives in each group), as follows: Fe0 (no Fe supplementation), and Fe25, Fe50, and Fe100 (supplemented with 25, 50, and 100 ppm Fe, respect...

Background: The nutritional requirements of honeybees (Apis mellifera) for their complete development need to be supplied through food sources available in the environment, since honeybees are insects that depend directly on blossoming food sources. However, at certain times a food-supply reduction can promote nutritional stress, thus necessitating...

Honey bees (Apis mellifera L.) have great global socioeconomic and environmental importance. However, the greater wax moth (Galleria mellonella L.) is a pest that causes serious worldwide damage to honey bee colonies. Good beekeeping practices and physical, chemical, or natural methods can be used to control wax moths. The use of natural products i...

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyraclostrobin and the insecticide fipronil) at field...

The antibacterial activities of apitoxin, a venom produced by Apis mellifera bee, and melittin, an antimicrobial peptide from apitoxin, were tested against planktonic and biofilm states of Staphylococcus aureus methicillin-resistant (MRSA), including clinical, and enterotoxin-producing isolates. Also, the synergism of apitoxin and melittin in combi...

Bee bread is the main source of proteins necessary for the development of hypopharyngeal glands in nurse bees. However, the seasonal chemical composition and its effects on honey bee physiology are poorly understood. Thus, this study evaluated how the season and botanical origin of pollen influence the content of crude protein, ash, and total flavo...

ABSTRACT This study evaluated the in vitro toxicity and motor activity changes in African-derived adult honey bees (Apis mellifera L.) exposed to lethal or sublethal doses of the insecticides fipronil and imidacloprid. Mortality of bees was assessed to determine the ingestion and contact lethal dose for 24 h using probit analysis. Motor activities...

We aimed to evaluate the frequency of bee pollen production, its botanical origin and chemical composition when collected in different seasons. Our results indicate that higher proteins (22.80 ± 3.09%) and flavonoids (2789.87 ± 1396.00 μg 100g-1) levels were obtained in the winter season, which also showed greater pollen production (134.50 ± 35.70...

The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0...

Apitoxin harvesting is a stressful practice for honey bees Apis mellifera L. due to the release of alarm pheromones that alter communication and behaviour and may also affect their physiology. Thus, the goal of this research was to verify the effects of apitoxin harvesting on the development of the hypopharyngeal glands (HGs), evaluating the number...

Honey bees are important pollinators and are essential in beekeeping. Honey bees get exposed to systemic pesticides while foraging in contaminated fields, and it is important to know the toxicity (LD50) and evaluate the impacts of bees’ exposure to these molecules. Fipronil and imidacloprid are systemic pesticides widely used in Brazil and other co...

Background: Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods: Africanized honeybee venom from Apis mellifera was fractionated by RP...

This research evaluated beeswax production by Africanized honeybees, using essential oils: citronella, eucalyptus, lemongrass and fennel. The sheets of beeswax received 0.5 ml of each oil, on both sides, and placed in the hive. The measurement of constructed area was analysed weekly and the treatment that had a significant expand was evaluated the...

Propolis is a substance produced by bees from plant sources located in the vicinity of the bee hives and it has a wide spectrum of biological properties. Brazilian propolis stands out due to its unique characteristics. In the 1990s, some Brazilian beekeepers started specializing in propolis production due to growing commercial demand for this produ...

The biological properties of apitoxin have prompted its production for use in human and animal health applications. However, the apitoxin harvest triggers a defense reaction in honeybee colonies, which includes the release of alarm pheromones (isopentyl acetate and 2-heptanone), which cause stress and could cause behavioral changes that influence t...

The effects of seasonal variations and the methods of collection of propolis produced by Africanized honey bees Apis mellifera Linnaeus, 1758, on the composition of constituent minerals such as magnesium (Mg), zinc (Zn), iron (Fe), sodium (Na), calcium (Ca), copper (Cu), and potassium (K) were evaluated. Propolis was harvested from 25 beehives by s...

In this study, samples of coffee honey produced in Espírito Santo State, Brazil, were characterized based on their melissopalynology, physicochemical and nutritional properties, and mineral and caffeine contents. The caffeine content in the nectar from coffee flowers was measured by high performance liquid chromatography (HPLC). Blends of honey wer...

To determine the dietary β-glucan and vitamin C levels that would enable the fish to cope with different stress, 252 tilapia (16.86 ± 0.24 g) were distributed in thirty six 250-L aquaria and fed experimental diets for 60 d. Diets were supplemented with β-glucan (0.1, 0.2, 0.4, and 0.8%) and vitamin C (400 and 600 mg/kg diet) and distributed in a fa...

The aims of this study were to determine the period effects (morning and afternoon) and harvest time (30 and 60 minutes) in the apitoxin production as well as the management effects in the expression of a stress related gene. Five Apis mellifera L. beehives were used. The harvest of apitoxin and honeybees occurred three times a week (morning and af...

The effect of different types of energy feeding (sugar syrup, inverted sugar and juice of sugar-cane) on beeswax production and its economic feasibility are evaluated. Twenty beehives of Africanized Apis mellifera were selected, and five were used for each type of feeding. The treatments were T1 (sugar-cane juice), T2 (sugar syrup) and T3 (inverted...

Bees play a crucial role in pollination and generate honey and other hive products; therefore, their worldwide decline is cause for concern. New broad-spectrum systemic insecticides like fipronil can harm bees, and their use has been discussed as a potential threat to bees' survival. In the present study, the authors evaluated the in vitro toxicity...

Vitamin A (vitA) is an essential nutrient that acts as an endocrine regulator of several metabolic pathways, modulating normal growth and health status of animals. Although the importance of vitA for normal haematology and immune response is well documented for higher vertebrates, there is limited information on the physiological effects of vitA on...

This study aimed to generate a database related to the spatial distribution of hives and productivity of regional producers, through the Geographic Information System (GIS) using the Global Positioning System. To build the database of geographical information (GIS) software for processing of geo-referenced information was used. The microregion (4 m...

The aim of our study was to investigate how the collection period affects and influences the production, chemical composition, and size of bee pollen loads (0.5, 1.0, 2.0, greater than 2.0 mm). The results showed there was a predominance of pollen loads with a diameter greater than 2.0 mm in all the production seasons. For all the seasons, there we...

Introduction: This study aimed to investigate the occurrence of Africanized honeybees in Botucatu, São Paulo, Brazil, and to implement a program to remove such swarms. Methods: The occurrences of Africanized honeybee swarms between 2010 and 2012 were studied and strategies to prevent accidents were developed. Results: We noted 1,164 cases of A...

This study aimed to generate a database related to the spatial distribution of hives and productivity of regional producers, through the Geographic Information System (GIS) using the Global Positioning System. To build the database of Geographical Information (GIS) software for processing of geo-referenced information was used. The microregion (4 m...

Objetivou-se gerar uma base de dados relacionada à distribuição espacial, de colmeias e produtividade dos apicultores regionais, por meio do Sistema de Informações Geográficas (SIG) utilizando-se o Sistema de Posicionamento Global. Para a construção do banco de informações foi utilizado o software de processamento de informação georreferenciada. A...

The present study aimed to evaluate the effect of propolis use on hematological and serum biochemical parameters in Blue-fronted Amazons (Amazona aestiva). For this, 12 adult birds were distributed randomly into individual cages, divided into treatments with different propolis levels (A = 0.0%; B = 0.5%; and C = 1.0%), in 3 distinct phases (I, II,...

The antibacterial activity of propolis produced throughout the year by different methods of collection ('intelligent' collector of propolis - ICP; plastic screen - PC; conventional scraping - CS) on Staphylococcos aureus and Escherichia coli is investigated. Fifteen beehives (five per collector) of Africanized Apis mellifera were used. Monthly prod...

Agrochemicals on crop cultivated areas is a source of contamination for bees and may cause physiological and behavioral disorders and mortality. The LD50 of the pesticides fipronil and imidacloprid was determined and their effect on the learning behavior of Apis mellifera L. honeybee evaluated. LD50 was determined by the ingestion of contaminated f...

The pollen spectrum of propolis is a good indicative of the phytogeographical origin of the bee product and the local flora available for bee visiting. The present study aimed to perform pollen analysis of propolis produced by Apis mellifera L., in the São Paulo State, Brazil. Propolis samples were directly obtained from beekeepers and slides were...

This research evaluated a commercial dietary nucleotide supplement for Nile tilapia diet. Eight groups of seven fish (4.74 ± 0.03g) were fed nucleotide mixture diets (0.0; 0.5; 1.0; 2.0 and 4.0 g kg-1 diet) for 60 days. Growth performance, haematological and immunological parameters of seven fish/treatment were analyzed. Then, 60 fish were submitte...

O presente estudo teve por objetivos contribuir com a caracterização da produção apícola no Estado de Pernambuco e avaliar a qualidade físico-química de méis produzidos na região. Para isto, aplicou-se um formulário dirigido ao órgão representativo da classe apícola sobre aspectos produtivos e técnicos dos apicultores. Foram obtidas 14 amostras de...

The present study had as objective contribute to the characterization of beekeeping in the Pernambuco State and evaluate the physical-chemical quality of the honeys produced in the region. For this, was applied a directed formulary to the representative organ of beekeeping class about the productives and technicals aspects of beekeepers. Was obtain...

The increasing demand for propolis has caused a raise in its production. However, an increasingly pesticide-dependent agriculture is a great concern with regard to bees, their produce and environmental contamination. Current analysis evaluates the presence of pesticides (organochlorines, organophosphates, pyrethroids, carbamates, herbicides, fungic...

The use of conventional anthelmintic has been a growing concern among consumers who have become more and stricter toward drug residues in products of animal origin. Thus, this research aimed to evaluate the anthelmintic effect of alcoholic extract of propolis, at 30% of concentration, in naturally infected calves. Twelve calves weighting 60 kg and...

Bovine mastitis is considered the main disease causing great economic losses in dairy herds. It is usually treated by antimicrobial chemicals that promote drug resistance, residues in food and environmental contamination. However, consumers in different countries requires more natural foods and with higher quality. Thus, the objective was to check...

Objetivou-se neste trabalho avaliar a aplicação de própolis e cera vegetal na qualidade pós-colheita do abacate ‘Hass’. Os frutos selecionados foram submetidos aos tratamentos (1) sem cera e sem própolis, (2) com cera vegetal, (3) extrato alcoólico de própolis 100%, (4) cera e etanol 70%, (5) extrato alcoólico de própolis 2% e cera, (6) extrato alc...

The objective of this work was to evaluate the effects of oxalic acid and essential oils of plants on the control of Varroa destructor infestation in Africanized Apis mellifera colonies. The experiment was carried out in a completely randomized design, in 30 colonies, with six treatments and five replicates. Beehives were treated with essential oil...

Cited By :1, Export Date: 21 July 2015, Correspondence Address: Peixoto, E.C.T.M.; UENP - BandeirantesBrazil; email: emellopeixoto@uenp.edu.br, References: Andrade, U.V.C., (2010) Potencial antibacteriano do extrato hidrossolúvel de própolis obtido por hidrólise alcalina para a inibição de cultivos de Staphylococcus aureus e higienização de pré e p...

The use of conventional anthelmintic has been a growing concern among consumers who have become more and stricter toward drug residues in products of animal origin. Thus, this research aimed to evaluate the anthelmintic effect of alcoholic extract of propolis, at 30% of concentration, in naturally infected calves. Twelve calves weighting 60 kg and...

Propolis is a honeybee product that has been used since ancient times because of its therapeutic effects. It can be used in the development of alternative therapies for the treatment of many diseases, and because propolis shows antibacterial action, this work was carried out in order to investigate a possible synergism between propolis and antibiot...

Salmonella enterica serovar Typhi is the causative agent of typhoid fever in humans, and the use of antibiotics is essential for controlling this infection; however, the excessive use of antibiotics may select resistant strains. Propolis is a honeybee product and its antimicrobial activity has been intensively investigated. Thus, the objective of t...

The objective of this work was to typify, through physicochemical parameters, honey from Campos do Jordão’s microrregion, and verify how samples are grouped in accordance with the climatic production seasonality (summer and winter). It were assessed 30 samples of honey from beekeepers located in the cities of Monteiro Lobato, Campos do Jordão, Sant...

O objetivo do presente trabalho foi avaliar o efeito da sazonalidade sobre algumas características físico-químicas (extrato seco, flavonóides totais, pH e atividade de oxidação) dos extratos alcoólicos (EAP) da própolis obtida mensalmente, durante um ano, por diferentes técnicas (coletor de própolis inteligente, tela plástica e raspagem) em quinze...

The aim of this work was to investigate monthly, for one year the seasonality effect on the physicochemical properties (dry weight, flavonoids contents, pH and antioxidant activity) of ethanolic extract (EAP) of propolis produced by three different techniques (intelligent propolis collector, plastic screen or scraping) fifteen colonies of africaniz...

Os objetivos do estudo foram investigar a influência de produtos naturais como capim-limão (Cymbopogon citratus), folhas de abacateiro (Persea americana), casca de café (Coffea arabica) e sementes de mamona (Ricinus communis) na defensividade de Apis mellifera, e avaliar o efeito destes produtos no desenvolvimento populacional da colmeia. O comport...

Apis mellifera venom is comprised basically of melittin, phospholipase A(2), histamine, hyaluronidase, catecholamine and serotonin. Some of these components have been associated with allergic reactions, amongst several other symptoms. On the other hand, bee mass stinging, caused by Africanized honey bee (AHB), is increasingly becoming a serious pub...

Apis mellifera, the European honey bee, is perhaps the most studied insect in the Apidae family. Its venom is comprised basically of melittin, phospholipase A(2), histamine, hyaluronidase, cathecolamines and serotonin. Some of these components have been associated to allergic reactions, among several other symptoms. On the other hand, bee mass-stin...

Os objetivos do estudo foram investigar a influência de produtos naturais como capim-limão (Cymbopogon citratus), folhas de abacateiro (Persea americana), casca de café (Coffea arabica) e sementes de mamona (Ricinus communis) na defensividade de Apis mellifera, e avaliar o efeito destes produtos no desenvolvimento populacional da colmeia. O comport...

Equine antivenom is considered the only treatment for animal-generated envenomations, but it is costly. The study aimed to produce Apis mellifera (Africanized honeybee) and Crotalus durissus terrificus (C.d.t.) antivenoms using nanostructured silica (SBA-15) as adjuvant and cobalt-60 ((60)Co)-detoxified venoms utilizing young sheep. Natural and (60...