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Publications (29)
4526
Background: The American Cancer Society classifies Renal Cell Carcinoma (RCC) to be among the ten most common cancers. However, in spite of this need, effective biomarkers for early-stage detection of RCC are not yet available. The purpose of this study was to assess the diagnostic characteristics of a carcinoma-specific transmembrane N-glycop...
3038
Background: A double-blind clinical study was conducted on 304 clinically verified blood serum samples, including 141 non-small cell lung cancer (NSCLC), 133 healthy volunteers and 30 patients with chronic obstructive pulmonary disease (COPD). The objectives of the study were: The assessment of various cancer markers and their possible combina...
The main objective of this study was the characterization of preclinical tumor models based on their expression of alpha-fetoprotein receptor (RECAF) for targeting cancer cells with a new non-covalent complex (AIMPILA) containing alpha-fetoprotein as the carrier and Atractyloside as an apoptosis-inducing agent. For that purpose, we measured the amo...
ook Description:
Alpha-fetoprotein (AFP) has many applications across various disciplines. This oncofetal protein is routinely used by clinicians for antenatal testing as well for the screening and treatment monitoring of certain malignances. Innovative research using AFP has also been very promising. Recent developments include the use of AFP or...
Alpha-fetoprotein (AFP) was the first oncofetal antigen of clinical significance. Over 20000 papers have been published on this protein, covering from its physiology to its applications for cancer diagnosis and management. AFP has also been used as a tool to study cell differentiation and malignant transformation. During the course of those studies...
Ovarian cancer can be cured in up to 90% of cases if diagnosed early. CA125, the most studied ovarian cancer biomarker, exhibits poor sensitivity for detecting early disease stages and low specificity to malignancy. RECAF, the alpha-fetoprotein receptor, is a wide-spectrum oncofetal antigen with clinical potential for cancer diagnosis, screening, a...
Accumulating evidence based on alpha-fetoprotein (AFP) cell binding and uptake has shown the presence of a receptor for AFP on the surface of fetal and neoplastic cells. In order to further study this receptor, monoclonal antibodies (MAbs) made against pooled human mammary tumor membrane extracts were screened for their ability to inhibit the bindi...
A program has been developed for the modelling of modifications in DNA ends, for the construction of ligated junctions, and
for the analysis in these junctions of new restriction enzyme recognition sequences. This program allows the analysis of restriction
enzyme specifities in ligated junctions of cohesive or blunt DNA ends. Cohesive ends are cons...
Several serum proteins were immunohistochemically studied in benign and malignant breast lesions in 13 patients. The majority of infiltrating duct and lobular carcinomas showed albumin and IgG abundant immune reactive cells. Fewer cells contained immune reactive alpha-1-antitrypsin and transferrin. Staining was diffuse and limited to the cell cytop...
Several reports have shown a high percentage of homology between the primary structures of alpha-fetoprotein (AFP) and serum albumin (Alb). With the aid of a program which searches for all identical sequences, we compared the aminoacid sequence of AFP and Alb from several species. Two short pieces of very high homology were always present in all th...
The in vivo uptake by neuroepithelial cells of rat serum proteins injected into the mesencephalic cavity of developing chicken was demonstrated by immunohistochemical methods. It was found that 48 h after inoculation, rat alphafetoprotein (AFP) and serum albumin (SA) localize in the same places as do chicken AFP and SA. No labelling was present for...
The ability to internalize alpha-fetoprotein (AFP) and serum albumin, which is characteristic of embryonic and fetal elements undergoing differentiation, may reappear in some cultured neoplastic cells (i.e., the MCF-7 human breast cancer cell line). The in vivo uptake of AFP by spontaneous carcinomas of the CH3/Bi mouse was investigated. Nineteen m...
Evidence is presented for the existence of specific receptors for alpha-fetoprotein on the surface of MCF-7 human breast cancer cells. At 4 degrees C, the binding of alpha-fetoprotein to these cells displayed a biphasic saturation curve. Scatchard analysis revealed the presence of at least two binding sites with dissociation constants of 4.5 X 10(-...
Proteins from cerebrospinal fluid of chick embryos at 4-19 days of development were qualitative and quantitatively analyzed. The total protein concentration and the relative concentration of each protein fraction were calculated for each day. The main proteins found along development were immunoglobulin G, transferrin, alpha-fetoprotein, serum albu...
Alpha-fetoprotein (AFP) is taken up by developing fetal cells but not by normal adult cells. This finding together with the resemblance between fetal cells and cancer cells suggests that malignant tissue could incorporate AFP in opposition to normal tissue. In the present work, we injected mice bearing spontaneous mammary adenocarcinomas with 131I-...
The presence of α-fetoprotein (AFP) and serum albumin (SA) has been described within developing brain cells of mammals and chicken. In order to avoid the influence of the choroid plexuses in the transport, via cerebrospinal fluid, of these proteins to brain cells, we studied the presence of AFP and SA within chicken sensory ganglion cells during on...
Radiolabelled alphafetoprotein (AFP) was selectively concentrated by spontaneous mammary carcinomas of the Mouse. External photoscans of Mice injected with 131I-AFP confirmed the significant accumulation of the protein in the tumors. These results prove the usefulness of AFP as a radiotracer for mammary carcinomas and represent a novel approach for...
Interest in research on α-fetoprotein (AFP) began in 1963, when Abelev and his coworkers discovered the reappearance of this fetal globulin in the sera of mice bearing primary hepatomas. Since these data appeared, a great number of studies have been done on the association of AFP, as well as of other oncofetal antigens, with neoplasia. With regard...
The intracellular presence of alpha-fetoprotein (AFP) and serum albumin (SA) has been recently demonstrated in the developing brain of primates, rats and mice. We report here the morphologic localization by immunocytochemical methods of AFP, SA, ovalbumin (OA) and immunoglobulin G (IgG) in the nervous system of chicken embryos. AFP was detected ins...
Questions
Question (1)
I wrote a chapter for a book published by Nova. My article, which is Open Access (the book is not) appears in ResearchGate as: Alpha-Fetoprotein: Functions and Clinical Applications. www.novapublishers.com/catalog/product_info.php?products_id=57894 , but Nova changed their website and now the book and the chapter have a different link address than the former one in ResearchGate. I need to edit and change the current link (above) to the new one (https://novapublishers.com/wp-content/uploads/2018/10/978-1-63484-875-6_ch4.pdf). How can I do that? Regards, Ricardo
