Ricardo Machado Kuster

• Dr
• Professor (Associate) at Federal University of Espírito Santo

In order to enhance the chemical and biological understanding of the genus Erythrina, this study evaluated the chemical composition, phytotoxicity, and antioxidant potential of the hexane (Hex), dichloromethane (DCM), and ethyl acetate (EtOAc) phases from the methanolic extract of E. speciosaleaves. The DCM and EtOAc phases exhibited significant an...

Products derived from the latex of Euphorbia tirucalli were obtained through hydrolysis and column chromatography, resulting in products rich in triterpenes, ingenol 3-esters (I3E), and other derivatives from hydrolysed latex. These products underwent evaluation for their cytotoxic activity against gastric adenocarcinoma cells (AGS). Triterpene der...

Epicuticular wax from the leaves of Tillandsia stricta Sol. Sims and Tillandsia usneoides (L.) from Grumari, Marica and Jurubatiba, Rio de Janeiro sandbanks was extracted into chloroform and n-hexane for analyses. The extracts were subjected to gas chromatography (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS) analyses. In th...

No Brasil, por diversos motivos, doenças como Dengue, Chikungunia e Zika causadas por vetores, mosquitos contaminados com arbovirus, estão fora de controle. Devido às modificações edafoclimáticas, Aedes aegypti vem sendo encontrado em outros países da América Latina, onde ocorreram os incrementos da taxa de notificação de Dengue. Ademais, também de...

This article represents the historical record of the stages of the third management of the intercultural didactic garden (medicinal and sensory) in a courtyard used by the Homeopathy Service, located in the 7th Infirmary of the Hospital Geral Santa Casa da Misericórdia in Rio de Janeiro/Brazil, whose elaboration was inter - transdisciplinary and in...

The present article describes historic evolution conception process of an intercultural medicinal plant garden as a tool to construct social ecoperception about environmental health and cultural diversity, to improve environmental ethics. Through case study methodology it reports the process along five years. Hybrid Learning of Homeopathy, Phytothe...

Eugenia uniflora leaves are a source of flavonoids and ellagitannins, and the Brazilian population uses them to treat various diseases, including gastrointestinal disorders. This study aimed to determine if the ethanol extract and other derivatives are effective cytotoxic agents against gastric adenocarcinoma (AGS cells) and anti-H. pylori agent, i...

Ethanol extract from the aerial parts of Euphorbia tirucalli L. as well as the latex of the plant suspended in water are used by the Brazilian population for the treatment of various diseases, including cancer. The purposes of this study were to determine if the ethanol extract is effective as cytotoxic agent against gastric adenocarcinoma cells (A...

This study evaluated the effect of hydroalcoholic extract from avocado (Persea americana Mill.) seeds (SCE), and its fractions, ethyl acetate (SEAP) and hexane (SHP), against Helicobacter pylori infection, its pathological process, and adenocarcinoma cells. Treatment with SEAP and SHP in H. pylori strains showed MIC and MBC ranging from 128 to 256...

Opioid receptors mediate antinociceptive effects. Methanolic fractions from sugarcane varieties (MFSCf) were evaluated in classic nociception models. Interactions between bioactive compounds and the µ-opioid receptor (µOR) through docking analysis were also studied. Five methanolic fractions of sugarcane juice were obtained and analysed by LC-ESI-M...

The latex of Euphorbia tirucalli L. (Aveloz) is popularly used to treat cancers from diverse sources such as: breast, prostate, lung and kidney. Furthermore, high dilutions of this latex (latex-HDs) have been successfully used in the treatment of tumors, although the mechanisms involved in this antitumoral activity are not yet known. The present st...

The latex extracted from Euphorbia tirucalli, a plant popularly known as Aveloz, is used in complementary medicine to induce tumor regression. However, as this latex has toxic effects when administered orally in ponderal doses, the present study was designed to assess the effects of high dilutions in healthy mice over a period of 18 weeks. The Avel...

Background The arthropod-borne Mayaro virus (MAYV) causes “Mayaro fever,” a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitte...

Brazilian traditional medicine has explored the antiviral properties of many plant extracts, including those from the Brazilian pepper tree, Schinus terebinthifolius. In the present study, we investigated the chemical composition and anti-mayaro virus (MAYV) activity of S. terebinthifolius fruit. Extensive virucidal activity (more than 95%) was det...

Revista Fitos, Rio de Janeiro. 2021; Ahead of Print | e-ISSN: 2446-4775 | www.revistafitos.far.fiocruz.br | CC-BY 4.0 ARTIGO DE PESQUISA In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract Abstract This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic...

Flowers from Dendranthema x grandiflorum (Ramat.) Kitam. are grown in Brazil for floristic trade, and residues from different cultivars are generated, containing flowers, leaves and stems. These residues can be used to produce larvicidal products. To confirm this hypothesis, 27 dichloromethane extracts were produced from Dendranthema x grandiflorum...

Background: The arthropod-borne Mayaro virus (MAYV) causes ‘Mayaro fever’, a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. Currently, there are no licensed drugs against most mosquito-transmit...

Residues of the chrysanthemum agroindustry from different cultivars of Dendranthema x grandiflorum are sold in the Vitória (Espírito Santo State, Brazil) marketplaces. Because of the large of varieties of this hybrid species, this study aimed to confirm the chemical similarity of the residual material collected in one year at the commercial places...

As plantas Plantago sp, Schinus terebinthifolius, Mikania glomerata e Mentha sp são listadas pela RENISUS com interesse de inseri-las como recursos terapêuticos para a população. Assim, o objetivo do presente estudo foi realizar análises químicas que identifiquem os principais metabólitos secundários e avaliar a atividade antioxidante de tinturas e...

Dengue is the most important infectious disease in the world and is a severe public health problem. The chikungunya is an arbovirus, in many cases, increased, which is transmitted by the same transmitter dengue vector, Aedes aegypti. The symptoms of both diseases are similar, and infections can be lethal. Although there is no preventive vaccine aga...

Neste estudo as partições diclorometano (DCM) e acetato de etila (AcOEt) do extrato metanólico bruto das folhas de P. heptaphyllum foram avaliadas quanto à composição em flavonoides, ao teor de fenóis totais (TFT) e ao potencial de redução in vitro do radical DPPH.

Saccharum officinarum L., Poaceae (sugarcane), occurs in tropical and subtropical areas of the planet, and Brazil stands out as the largest producer of sugar and ethanol from sugarcane. In addition, it is rich in phenolic substances, which have antioxidant potential, generating interest in its use in the cosmetic area. The objective of this work wa...

Espécies do gênero Erythrina representam um papel importante na medicina popular de países diversos, onde são utilizadas tradicionalmente no tratamento das mais variadas doenças, incluindo infecções, malária, inflamações, asma, bronquite, depressão, dentre outras (CUI et al., 2008). Erythrina speciosa Andrews é espécie nativa e endêmica do Brasil,...

Vigna unguiculata (L) WALP conhecida como feijão Caupi, também é conhecida na região nordeste e norte do Brasil como Feijão de praia, por ser cultivado em várzeas, devido à sua tolerância a ambientes salinos, favorável ao seu desenvolvimento vegetal. Contudo, em função do seu difícil cultivo, associado ao alto teor nutricional, investigações para i...

The deregulation of the Wnt/β-catenin signaling pathway is a central event in colorectal cancer progression, thus a promising target for drug development. Many natural compounds, such as flavonoids, have been described as Wnt/β-catenin inhibitors and consequently modulate important biological processes like inflammation, redox balance, cancer promo...

Background The current research aimed to explore secondary metabolites and antibacterial activity against bacterial agents associated with bovine mastitis from extracts prepared from the peel of fruits of Punica granatum L.. Mastitis is an inflammation in the mammary gland mainly due to infection of bacteria and can be presented clinically or subcl...

Helicobacter pylori is a bacterium that reaches half of the world population and it’s recognized as the main cause of chronic gastritis and peptic ulcer. In this study, we evaluated the anti-H. pylori, antioxidant and immunomodulatory activities of the methanolic (MeOH) extract of Eugenia uniflora leaves and chemical profile. Anti-H. pylori activit...

This study aimed to use different types of ion source gases [synthetic air, nitrogen (N2), and helium (He)] to compare the ionization efficiency of linear, branched, and cyclic hydrocarbon (HC) standards (i.e., hexatriacontane, squalene, and 5-α-cholestane, respectively), condensed aromatics (CA) standards (i.e., coronene, benz[a]anthracene, and n,...

Brazilian oil production of pre-salt layer consists of oils with high content of saturated compounds. The identification of paraffinic and naphthenic compounds in these crude oils is key to understanding their rheological behavior. In the present study, comprehensive two-dimensional gas chromatography was performed to assess the greater separation...

Many studies have reported on the use of Euphorbia species against cancer. Tinctures of Euphorbia tirucalli L., popularly known as aveloz, have been widely used in Brazilian traditional medicine to treat cancer. However, the influence of seasonal variations on the cytotoxic activity of E. tirucalli ethanolic extracts (tinctures) has not been invest...

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversedphase HPLC techniques the rutin and kaem...

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversed-phase HPLC techniques the rutin and kae...

The Cannabis sativa L. plant has a complex chemical composition, containing various chemical compounds such as terpenes, sugars, hydrocarbons, steroids, flavonoids, and amino acids. Few works have attempted to identify the constitutional isomers of cannabinoids that are found in the plant. The present study reported the analysis of seven cannabinoi...

Ethanol extracts obtained from Schinus terebinthifolius Raddi fruits and leaves were active against Escherichia coli with MIC of 78 μg mL⁻¹ for both extracts. Phytochemical analyses revealed a major presence of phenolic acids, tannins, fatty acids and acid triterpenes in the leaves and phenolic acids, fatty acids, acid triterpenes and biflavonoids...

Purpose: To investigate Euphorbia tirucalli extract for probable geographic variations in its antiproliferative activity. Methods: The aerial parts of E. tirucalli were collected in the Brazilian states of Mato Grosso, Rio de Janeiro, Pará, Minas Gerais and Santa Catarina. The 70 % ethanol extract was obtained according to the procedure described i...

Poly (butylene succinate) (PBS) is a biodegradable polyester, which can be obtained from renewable sources. The chain extension technique was used, aiming to promote an increase in the molecular weight of PBS, besides improving its thermal, mechanical and rheological properties. To the best of our knowledge, the use of rutin as a natural chain exte...

The first natural occurrence in optically active form of the dimeric flavonoid agathisflavone and definition of its axial chirality using chiroptical spectroscopic methods are described. The experimental electronic circular dichroism, electronic dissymmetry factor, optical rotatory dispersion, vibrational circular dichroism (VCD), and vibrational d...

Marijuana is one of the most marketed illicit drugs in the world. Originated from Cannabis sativa L, Δ⁹-tetrahydrocannabinol (or Δ⁹-THC) is proscribed in Brazil by the law number 11.343 from August 23rd of 2006 as well as its isomers, salts, ethers and esters. However, Δ⁹-THC is the main responsible for the hallucinogenic effects of the plant. The...

The synthetic cannabinoids (SCs) represent the most recent advent of the new psychotropic substances (NPS) and has become popularly known to mitigate the effects of the Δ(9)-THC. The SCs are dissolved in organic solvents and sprayed in a dry herbal blend. However, little information is reported on active ingredients of SCs as well as the excipients...

In hybrid cultivated form, Dendranthema grandiflorum (Ramat.) Kitam., Asteraceae, flowers (Chrysanthemum morifolium Ramat.) were utilized in the production of extracts, which were analyzed for larvicidal activity against Aedes aegypti third instar larvae. Methanol and dichloromethane extracts showed LC50 values of 5.02 and 5.93ppm, respectively. Us...

In models of metabolic disorders, cinnamon improves glucose and lipid metabolism. This study explores the effect of chronic supplementation with aqueous cinnamon extract (CE) on the lipid metabolism of rats. Male adult Wistar rats were separated into a control group (CTR) receiving water and a CE Group receiving aqueous cinnamon extract (400 mg of...

This paper describes the correlation between the phenolic composition and the molecular phylogenetic reconstruction of five Spondias species (Anacardiaceae). Two of these species (S. venulosa and Spondias sp.) occur in rainforest areas and the other three are widely distributed in Brazil (S. dulcis, S.mombin, and S. purpurea). The flavonoid enriche...

Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as wel...

Euphorbia tirucalli aerial parts are popularly used in Brazil for cancer treatment. The elution of the aqueous extract of the plant on silica gel C-18 cartridge furnished a water-soluble fraction, which was analyzed directly into the electrospray ionization (ESI) source combined with Fourier transform ion cyclotron resonance mass spectrometry (FT-I...

Overactivation of the Wnt/β-catenin pathway in adult tissues has been implicated in many diseases, such as colorectal cancer. Finding chemical substances that can prevent this phenomenon is an emerging problem. Recently, several natural compounds have been described as Wnt/β-catenin inhibitors and might be promising agents for the control of carcin...

One of the Brazilian arnicas, Solidago chilensis Meyen, is a species of the Asteraceae family. This plant is known by this common name because it shares remarkably similar organoleptic properties with the genus Arnica L., also within the family Asteraceae. We examined the effectiveness of the S. chilensis fluid extract used externally for treating...

Phyllanthus tenellus Roxb. is a widely distributed tropical medicinal plant and highly valued for its therapeutic properties. Since variable light conditions can significantly alter phenolic compounds that are the main therapeutic constituents of P. tenellus, including tannins and flavonoids, the development of this plant and its chemical metabolis...

Background The arthropod-borne Mayaro virus (MAYV) causes `Mayaro fever¿, a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Studies showed that the virus could also be transmitted by the mosquito Aedes aegypti. Recently, MAYV has attracted attention due to its like...

Extracts of Echinodorus grandiflorus obtained from dried leaves by three different techniques were evaluated by bacterial lysogenic induction assay (Inductest) in relation to their genotoxic properties. Before being added to test cultures, extracts were sterilized either by steam sterilization or ultraviolet light. Only the extracts prepared by inf...

Ethnopharmacological relevance: Prostate cancer is one of the most diagnosed forms of cancer among men in western regions. Many traditional applications or phytotherapeutic concepts propose to inhibit the proliferation of prostate cancer cells. In order to detect influences of plant or fungal extracts and derived fractions on androgen receptor sig...

The arthropod-borne Mayaro virus (MAYV) causes 'Mayaro fever', a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. Currently, there are no licensed drugs against most mosquito-transmitted viruses....

The present study aimed to investigate the in vitro effects of isoschaftoside isolated from Syngonium podophyllum on pig kidney Na+, K+-ATPase. The Na+, K+-ATPase activity was determined by colorimetric measurement of inorganic phosphate (Pi), resulting from ATP hydrolysis. Isoschaftoside significantly decreased the renal Na+, K+-ATPase activity at...

Cancer chemotherapy is an important strategy to treat this leading cause of death worldwide and plants may constitute a source of new antineoplastic agents. This work fractionated the ethanolic extract of Jacaranda puberula leaves and studied the in vitro antitumoral action and some toxicological effects of the most bioactive fraction. Cell lines r...

Chemical composition of Bonnetia stricta and chemosystematic significance. ► First report of anthraquinones in Bonnetia genus. ► First report of the C-glucosylxanthone (mangiferin) in Bonnetiaceae family.

In a search for potential acetylcholinesterase inhibitors (IAChE), the dichloromethane ex-tract from Sideroxylon obtusifolium leaves demonstrated a marked enzyme inhibition. A TLC bioauto-graphic assay and Ellman's reaction were used as bioassays to guide the isolation of IAChE. Three com-pounds were purified sufficiently to allow structural analys...

Bumelia sartorum (Sapotaceae) is used ethnomedicinally for treatment of several diseases, including diabetes mellitus. The aim of this work was to investigate the hypoglycemic effect of B. sartorum extracts, rich in polyphenolic compounds, and the possible mechanisms of action. Assessment of B. sartorum hypoglycemic activity was performed from the...

Maternal Wnt/β-Catenin signaling is essential to establish dorsal-specific gene expression required for axial patterning in Xenopus. Deregulation of this pathway causes axis phenotypes in frog embryos. In adult life, mutations in the Wnt pathway components are associated with many diseases, such as polyposis coli; osteoporosis-pseudoglioma syndrome...

Ethanol extract obtained from dried leaves of Acmella oleracea afforded after a liquid/liquid partition procedure a larvicidal hexane fraction (LC50 = 145.6 ppm) and a non larvicidal dichloromethane one. From the inactive fraction, three amides were identified, two new structures, named deca-6,9- dihydroxy-(2E,7E)-dienoic acid isobutylamide (1), de...

An aqueous extract and fraction from the marine sponge Petromica citrina have antibacterial activity. We performed a chemical and biological characterization of the antibiotic substance from P. citrina and investigated its mode of action on Staphylococcus aureus cells. The inhibitory activity of the aqueous extract of P. citrina was determined agai...

The allelopathic potential of Terminalia catappa L. Combretaceae fruits and leaves on Lactuca sativa L. (lettuce), Euphorbia heterophylla L. and Commelina benghalensis L. was studied. Bioassays indicated the highest activity for dichloromethane and ethyl-acetate fractions of ethanolic extracts from fruits, and the mean effective concentration (EC50...

Sideroxylon obtusifolium (Roem. & Schult.) T. D. Penn. (family Sapotaceae) is a tree native to Central and South America. Infusions of the bark and the leaves are used in Brazilian folk medicine as an anti-inflammatory remedy. However, information on the constituents of S. obtusifolium remains scarce, and only common pentacyclic triterpenoids have...

This work describes the phytochemical study of the methanol extract obtained from leaves of Guarea macrophylla, leading to the isolation and identification of three flavonoid glycosides (quercetin 3-O-β-D-glucopyranoside, quercetin 3-O-b-D-galactopyranoside, kaempferol 7-O-β-D-glucopyranoside) and a neolignan glucoside, dehydrodiconiferyl alcohol-4...

Alpinia zerumbet (Zingiberaceae) presents a high medicinal value, in part by its production of volatile compounds. Organogenic in vitro cultures of A. zerumbet were treated with the elicitor methyl jasmonate (MeJA), a natural volatile signaling molecule in biotic and abiotic stress, and the effect was evaluated on the in vitro production of volatil...

The ethanol extract of the vegetal species Pentaclethra macroloba (Willd.) Kuntze, Fabaceae, was fractioned and the antibacterial activity was determined. The active ethyl acetate (ea) fraction showed activity against Gram-positive (Staphylococcus spp. and Enterococcus spp.) and Gram-negative (Pseudomonas aeruginosa, Acinetobacter spp. and Klebsiel...

Highlights ► We showed the presence the flavonoid glycosides isolated from Myrsine rubra. ► Flavonols and flavones glycosides in the M. rubra is a tendency of evolution. ► Myrsinaceae genera have been described only flavonol glycosides. ► This is the first report of flavone and a flavonol glycoside in M. rubra.

Phyllanthus tenellus is widely used for its antiviral, analgesic and hepatoprotective properties. Although the production of several chemical classes of secondary metabolites is influenced by UV radiation, particularly phenolic compounds, we also know that UV radiation can result in anatomical and developmental damage. However, the morphological, a...

In order to validate the Bumelia sartorum Mart., Sapotaceae, traditional use for infection diseases, this study evaluates the antibacterial activity of the stem bark fractions against methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus strains by using the agar dilution method and reported as MIC (minimal inhibitory...

Volatiles produced by plantlets of Alpinia zerumbet were obtained by means of simultaneous distillation-extraction (SDE). The effects of indole-3-acetic acid, kinetin, thidiazuron and 6-benzylaminopurine on leaf and root volatile composition obtained by tissue cultures were investigated. A higher content of b-pinene and a lower content of sabinene...

Alpinia zerumbet plantlets were cultured in vitro in MS medium supplemented with growth regulators, including IAA, TDZ and BAP. Using high performance liquid chromatography (HPLC), the production of rutin, kaempferol-3-O-glucuronide, and kaempferol-3-O-rutinoside was evaluated, based on leaf hydroalcoholic extracts of three-month-old plantlets. The...

Background: although Hahnemann described the fifty-milesimal (LM) method in the 6th edition of the Organon of the Medical Art, very little research has been carried out on the physical chemical properties of these homeopathic preparations. Furthermore, there is still no evidence allowing for the correlation between the alleged physical chemical pro...

Echinodorus macrophyllus, commonly known as chapéu-de-couro, is a medicinal plant used in folk medicine to treat inflammation and rheumatic diseases. In this work, we used short-term bacterial assays based on the induction of SOS functions to examine the genotoxicity and mutagenicity of an aqueous extract of E. macrophyllus leaves. Whole extract an...

The antioxidant activity of leaf extracts from Jacaranda puberula Cham., Bignoniaceae, was assayed by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging method. Three phytomedicines (F1, F2, and F3) used as blood depurative, were tested by the same method. The free radical scavenger potential was measured by the discoloration of the...

The cell viability of Lepechinia speciosa (St. Hill) Epling fractions was measured by cell membrane integrity (lactate dehydrogenase assay) on rat basophilic leukemia cells (RBL-2H3). All fractions and extract tested (100 μg/ml) increased the release of lactate dehydrogenase (LDH), being the ethyl acetate and dichloromethane fractions with LDH rele...

Chemical composition of leaf volatiles of Rosmarinus officinalis and Baccharis dracunculifolia cultured in Southeast of Brazil has been characterized by GC/MS after simultaneous distillation-extraction. The main components in volatiles of these species showed in common α-pinene, myrcene, 1,8 cineole and camphor. Camphor was the major component amon...

The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromato...

Erva cidreira (Melissa officinalis) is a plant with sedative properties and in Brazil it has been used for insomnia and anxiety. It is also employed to stimulate liver functions and normalize menstruation and for intestinal constipation. The aim of this work was to evaluate the genotoxic and mutagenic effects of erva cidreira. Results indicate the...

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversed-phase HPLC techniques the rutin and kae...

Couroupitaguianensis Aubl. (Lecythidaceae) is popularly known in Brazil as "abricó-de-macaco". Infusions or teas obtained from its leaves, flowers, and barks are used in South America for the treatment of several disorders such as pain and inflammatory processes. Evaluate antinociceptive effects of crude ethanol extract (CEE) and its fractions in t...

Nowadays, the high blood pressure is one of the main causes of death and cardiovascular diseases. Vasodilator drugs are frequently used to treat arterial hypertension. Experiments were undertaken to determine whether hydroalcoholic extracts obtained from leaves of field-grown Alpinia purpurata and A. zerumbet cultured in vitro under different plant...

"Antiviral activity of Musa acuminata Colla, Musaceae." This study evaluates the antiviral activity of extracts and, fractions of Musa acuminata Colla collected in two regions of Rio de Janeiro State (Petropolis and Santo Antonio, de Padua). The inflorescences of M acuminata showed excellent activity for the two virus evaluated: simple human herpes...

The present paper describes the phytochemical and anti-staphylococcal activity investigation of the dichloromethane extract of the Brazilian plant Zizyphus joazeiro Mart. The purification steps were guided by bioassays against 17 bacterial strains of clinical sources, including methicillin-resistant (MRSA) and -sensitive (MSSA) Staphylococcus aureu...

. The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata through HPLC analysis. Cultures were maintained in liquid MS medium in the following treatments: MS0 (control), TDZ, BAP and IAA + TDZ. Kaempferol-3-O-glucuronide content was higher than rutin for all in vitro treatments, except by control medium in which pl...

The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as...

Three phloroglucinols were obtained from Hypericum brasiliense: japonicine A (1), isouliginosin B (2) and uliginosin B (3). Bioautography and disk diffusion methods were used to determine antibacterial activity of the hexanic extract. Strains of the Coagulase Negative Staphylococcus and American Methicillin Resistant Staphylococcus aureus clones sh...