Skills and Expertise
Research Items (22)
- Sep 2016
The present study was conducted to analyze the phylogenetic status of the genus Onobrychis and to evaluate the monophyly of its subgenera and sections and relationship among them. We sequenced the nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) and three chloroplast regions trnL-F, rpl32/rpl32-trnL(UAG) and ndhF-rlp32 for phylogenetic reconstruction of 51 species of Onobrychis. In all of our analyses, Eversmannia subspinosa, Corethrodendron scoparium, Greuteria membranacea and G. argyrea were chosen as outgroups. Phylogenetic analyses were performed by maximum parsimony, maximum likelihood and Bayesian methods. Our molecular data indicate that Onobrychis is monophyletic and composed of two main clades, each corresponding to the redefined subgenus Onobrychis (including sections Onobrychis and Hemicyclobrychis) and subgenus Sisyrosema (including sections Afghanicae, Laxiflorae, Heliobrychis, Hymenobrychis, Insignes, Lipskyanae and Litvinovianae), respectively. Sections Lipskyanae and Litvinovianae are newly established and described, representing distinct lineages within the genus. Onobrychis splendida, a species hitherto without a sectional position, along with some members of sect. Anthyllium were retrieved representatives of section Lipskyanae. Sections Afghanicae, Insignes, Heliobrychis and Hymenobrychis (with the inclusion of two species of section Anthyllium) are monophyletic. Sections Dendrobrychis and Lophobrychis are reduced to synonymy of section Onobrychis and Anthyllium to synonymy of section Hymenobrychis. A taxonomic treatment for the genus is presented.
This study is concerned with the vegetation investigation of Golestanak prohibited area, an ecotone with nearly 1800 ha area located between the timberline and subalpine-alpine zone in the central part of the Alborz Mountain Range (Mazandaran province). The effects of altitudinal gradient and aspect on plant diversity were evaluated by using CCA method (Canonical correspondence analysis) and a system of sixty sample plots were randomly used along five transects. The results showed that there is a negative correlation between the altitude and biodiversity variants meaning an increase in altitude leads to a decrease in the Shannon and Simpson indexes of the plots. The northern slopes have the highest plant diversity than the eastern and southern slopes, and the western slopes are allocated to the lowest plant diversity.
available on: http://www.abc.botanic.hr/index.php/abc/article/viewFile/1623/345 Chromosome numbers were determined in ten accessions of Phlomis olivieri Benth. (Lamiaceae). The seeds were collected from natural habitats in the west of Iran. Chromosome numbers of all accessions were 2n=2x=20. The chromosomes of accessions were metacentric or submetacentric, ranging in length from 2.66 to 8.604 μm. According to the average values of ten accessions, the karyotype of this species consists of 10 pairs of metacentric chromosomes. An ideogram was depicted for the species. This is the first report on the chromosome number and karyotype analysis of P. olivieri from Iran.
Semnan province is a vast and very diverse territory surrounded by Golestan, Mazandaran, Tehran, Qom, Isfahan and Khorasan provinces. The public image of Semnan province in the northern part, completely mountainous and in the southern part, is a desert, which indicates the existence of a great diversity in the ecological characteristics of the region. The present study was conducted to identify plant specimens of Semnan province in herbarium of Damghan University (DU) and to introduce halophytic species for planting them in other regions of the country. A total of 311 species belonging to 237 genera and 68 families from vascular plants were identified, of which, 8 species are categorized in the list of halophytic species. Among them, the Chenapodiaceae family with 5 species is rich in halophytic species. Halophytic species including Anabasis aphylla, Atriplex flabellum, Camphorosma monspeliaca, Salsola gossypina , S. kerneri, Phragmites australis, Prosopis farcta and Typha domingensis. The mentioned species has potential for cultivation and is recommended in saline areas.
Available on: http://tbj.ui.ac.ir/article_22631_fb3a21106b0ea99edde13f4b18d3150a.pdf The study is concerned with the floristic investigation of Sarband prohibited area (Dibaj). The region with an area of approximately 4000 hectares is located in 12 km northeast of Dibaj (52 km north of Damghan, Semnan Province) ranging from 2000-3650 m. Sarband area with an average annual precipitation of 137.5 mm a dry climate based on Domarten’s climate classification. A total of 285 species belonging to 178 genera and 45 families were identified. The richest families species were Asteraceae (22 genara, 40 species), Brassicaceae (25 genera, 32 species) and Poaceae (16 genera, 26 species), respectively. Also, the genus Astragalus with 12 species, Allium with 7 species, Cousinia and Alyssum each with 6 species and Gagea with 5 species, are the largest genera in the region. Phytogeographical distribution of plant species showed that 60.52% of species belong to the Irano-Turanian region, followed by Irano-Turanian/Europe-Siberia: 20.67% and etc. Hemicryptophytes (54.76% of all species) were the most common life form in the region. 36 Iranian endemic species and 44 medicinal plant species were recognized in the region. Moreover, 33 plant species were listed as threatened species, of which, four species including Cousinia rahbdodes, Astragalus catacamptus, A. megalocystis and A. perdurans were categorized as vulnerable species based on the criteria of the International Union for Conservation of Nature.
Sarband prohibited area is located in 12 km of NE Dibaj (Damghan, Semnan province). Based on a floristic study was performed in the region between 2014 and 2016, 285 species has been identified of which 33 of them are threatened species according International Union for Conservation of Nature (IUCN). Based on the classification of IUCN and Jalili & Jamzad’s study (1999), the threatened species of the region is composed of 4 vulnerable species, 22 species in low risk and 7 species with deficiency data. The vulnerable species are Cousinia rhabdodes, Astragalus catacamptus, A. megalocystis and A. perdurans. Also Asteraceae with 8 species and Fabaceae with 6 species are the biggest families in term of the amount of threatened species, respectively. The rest of families each contain 1 or 2 endangered species. Because of increasing the number of endangered species in the world and Iran, high richness of the flora, presence of 34 endemic species, 45 medicinal species, 4 vulnerable species and a large number of species in low risk a long with richness of fauna (personal observations) in the studied area; all are evidence that the prohibited area could have higher priorities of conversation. Therefore in order to manage biodiversity of the region, elevating its conservation level from a prohibited area to a protected area is proposed.
available on http://tbj.ui.ac.ir/article_22329_84b2748ada8a75b5cd33ef3c94790101.pdf This study is concerned with the morphological cladistic analysis of the monotypic tribe Bromeae including the genus Bromus. To characterize taxonomic circumscription of the genus, its species and sectional relationships, 39 taxa belonging to 6 sections and 2 outgroups were evaluated. Based on the heuristic search for 26 morphologhical informative characters, Maximum Parsimony approach was used for phylogenetic analyses. The data matrix was analyzed by reweighting characters method, rescaled consistency index and retention index. The results obviously indicate the monophyly of the genus Bromus and taxonomic circumscription of its sections but phylognetic relationships of many species within each section remain unresolved. The monotypic sect. Nevskiella (lemmas short, 3.5- 5 mm long and awns 3-5 times the length of lemmas) is clearly separated from other sections of the genus Bromus. Old world taxa of the perennial sect. Pnigma are more closely related to sect. Ceratochloa. The annual sects. Genea (spikelets cuneiform, broader at top) and Bromus (spikelets ovate, elliptic or lanceolate tapering towards top, lemmas with 1-5(6) awns) are monophyletic. The present cladistic analysis confirms the incorporation of the genus Boissiera (lemmas with 7-9 awns) into the genus Bromus.
The novel technique of identifying biological specimens using short DNA sequences from either nuclear or organelle genomes is called DNA barcoding. DNA barcoding not only helps in the identification of species but can also define species boundaries, flagging of new species and species delimitation [1,2]. The Consortium for the Barcode of Life Plant Working Group (CBOL)  evaluated seven chloroplast genomic regions across the plant kingdom and proposed a combination of matK and rbcL as plant barcodes. In closely related species, the discriminating ability of these two markers is low [4,5]. Therefore, the China Plant BOL Group  proposed the addition of nuclear ITS (Internal Transcibed Spacer) to the matK + rbcL combination as plant barcode in order to achieve maximum identification rates even in closely related species. Bromus L. with more than 151 species in the world comprises annual to perennial species. Bromus taxonomically is a complex genus with difficult nomenclatural history and many species are hard to distinguish due to their high degree of morphological similarity . 17 species sequences beloning to Bromus sect. Bromus were aligned with Muscle program and adjusted manually. Phylogenetic reconstruction was perforemed using maximum parsimony method with heuristic search and TBR branch swapping as implemented in PAUP* and Bayesian inference with GTR + G evolutionary model by MrBayes. We also constructed neighbour-joining trees based on the DNA sequences. Results from analysis of data showed that all species were recognized with nrDNA sequences and the sequences are an appropriate species marker for this genus.
A new taxonomic concept for Bromus danthoniae including comments on Bromus sectt. Bromus and Triniusia (Poaceae) Abstract Based on morphological analyses of the awns a taxonomic approach to Bromus sect. Bromus is presented. Based on the original publication B. macrostachys var. triaristatus and B. pseudodanthoniae are stated as homotypic synonyms of B. danthoniae. B. turcomanicus and B. danthoniae subsp. rogersii are treated as synonyms of B. danthoniae. Bromus sect. Triniusia as a synonym of Bromus sect. Bromus and the reduction of the genus Boissiera as synonym of Bromus are confirmed. Zusammenfassung Nach einer morphologischen Analyse der Grannen wird eine taxonomische Gliederung von Bromus sect. Bromus präsentiert. Basierend auf den Originalpublikationen sind B. macrostachys var. triaristatus und B. pseudodanthoniae homotypische Synonyme von B. danthoniae. B. turcomanicus und B. danthoniae subsp. rogersii sind synonym zu B. danthoniae, Bromus sect. Triniusia ist synonym zu sect. Bromus. Der Einschluss der Gattung Boissiera in Bromus wird bekräftigt.
The few of 300000 plant species in the world can be identified based on traditional methods. Classification and identification of plant species remains as a significant challenge for taxonomists. The emergence of DNA barcoding has had a positive impact on biodiversity classification and identification. In this approach, the species of life is recognized with speed, accuracy and low cost by using of short DNA fragment from a special and standard genome. Because of the importance of Fabaceae in human life, 22 sequences of the nrDNA ITS in Fabaceae members obtained from GenBank and analyzed by PAUP* software. Based on the results all of the species recognized with this marker. Therefore the nrDNA ITS recognize and present as an appropriate species marker in Fabaceae.
- Apr 2015
available on http://www.sekj.org/PDF/anb52-free/anb52-233.pdf The present study is a comprehensive taxonomic revision of the genus Bromus (Poaceae) in Iran. Based on the examination of 1500 herbarium specimens from Iran, 285 from neighboring countries and 30 type specimens, some nomenclatural modifications and classification changes are made. The tribe Bromeae for Iran was previously recognized as including the genera Bromus (29 species) and Boissiera (one species). Here, the number Bromus species in Iran is increased to 45. The Iranian taxa of the tribe Bromeae comprise the genus Bromus with 6 sections, 39 species and 40 taxa (two subspecies), including the cultivated B. catharticus, B. inermis and B. pumilio (synonym Boissiera squarrosa). Bromus rechingeri, B. pseudojaponicus and Bromopsis gabrielianae are reduced to synonymy. An identification key and detailed information of the geographical distribution of all treated species is provided.
The anatomy, palynology and trichome types of Phlomis olivieri were studied in order to understand the usefulness of these characteristics for systematic purposes. Results showed that vascular bundles were next to each other in the stem, the mesophyll was composed of 1-layered palisade and 3-4 layered spongy parenchyma. There were two collateral vascular bundles in the centre and two small subsidary bundles in the corners of petiole and there were 4 small vascular bundles in the bracteole. Five main types of trichomes (peltate, capitate glandular, stellate, unicellular simple and dendroid trichomes) were observed. The capitate trichomes were subdivided into three groups: type 1 (short stalk), type 2 (medium or tri-cellular stalk) and type 3 (long or four-cellular stalk). Stellate trichomes were subdivided into two groups: group 1, sessile or short stalked trichomes and group 2, long stalked trichomes. The dendritic trichomes also were reported for the first time in this species. The pollen grains were tricolpate, relatively large, ovate and the exine ornamentation was reticulate and perforate.
- Oct 2014
During work on a new synopsis on the genus Bromus L. for the ‘Flora Iranica’ region, an Afghan perennial brome grass previously identified as the Caucasian B. biebersteinii Roem. & Schult., was found to be a new species. It is described here as B. salangensis sp. nov. This discovery rejected the record of B. biebersteinii for Afghanistan.
Chemical composition of the essential oil of the fruit of Ferula assa-foetida L. obtained by steam distillation solvent extraction methodhas been studied by GC/MS for the first time. Fifty-four components, comprising 96.9% of the total oil, were identified.epi-α-Cadinol (23.15 %), germacrene B (10.98 %), α-gurjunene (6.18 %), (Z)-1-propenyl sec-butyl disulfide (5.89 %), 5-epi-7-epi-α-eudesmol (4.89 %), -cadinene (4.78 %), -cadinene (3.36 %) and germacrene D (3.09 %) were found to be the major constituents of the oil. The oil of the fruit of F. assa-foetidaconsisted of ten monoterpene hydrocarbons (6.14%), twenty-six sesquiterpene hydrocarbons (43.48%), nine oxygenated sesquiterpenes (37.77%), one oxygenated hydrocarbon (0.35%), and nine volatile sulfides (11.18%).
- Jan 2012
available on http://www.bio.bas.bg/~phytolbalcan/PDF/18_1/18_1_09_Naderi_&_al.pdf This study deals with the floristic and vegetation investigations of Golestanak Protected Area, an ecotone with nearly 1800 ha area located between the timberline and subalpine-alpine zone in the central part of the Alborz Mountain Range (Mazandaran Province). A total of 172 taxa, belonging to 39 families and 126 genera were identified, including 138 dicotyledones (99 genera) and 32 monocotyledones (25 genera). Based on a system of sixty sample plots taken randomly along five transects and using Sørensen's distance measure (Index BC=Bray and Curtis) and Ward's group linkage methods in a cluster analysis, nine plant communities were identified.
This article is a taxonomic review after Flora Iranica concerning some species of Bromus sect. Bromus. Comparing the holotype, isotype and paratype of B. pseudobrachystachys with the lectotype of B. brachystachys and also reexamination of three Iranian and Iraqi herbarium sheets putatively determined under the latter's name showed all belong to the former. B. racemosus, B. arvensis and B. secalinus are confirmed to occur in Iran. Despite B. tigridis was identified from Turkey based on the sheet Sorger & Buchner 82-62-54, the Iranian specimen was identified as B. racemosus. Furthermore, a new record of this section i.e., B. macrocladus is reported here from mountains of Razi village (W Khoy, NW Iran).
As a result of our study on the genus Bromus, B. rigidus and B. sewerzowii are reported as new records for the flora of Iran. Bromus diandrus-rigidus complex comprises a group of species ranging from tetra- to octaploid levels,which have been treated in different ways. Based on a specimen from Golestan National Park, B. rigidus is reported along with its illustration and a comparison with its closely related taxa. Furthermore, B. sewerzowii is the second record that is based on a specimen from North Khorasan (Sarakhs). In addition, a taxonomic key to two the species and their allies has been presented.
three new records of the genera Achillea, Cardaria and Scorzonera for the flora of Iran. -Iran. J. Bot. 15 (2): 167-171. Tehran. Three new records, Achillea millefolium subsp. sudetica (Asteraceae), Cardaria draba subsp. draba (Brassicaceae) and Scorzonera kirpicznikovii (Asteraceae) are reported for the first time from Iran. The taxa have been collected from Golestanak Protected Area in central part of Elburz (Alborz) mountains. Each taxon is compared to related taxon and its illustration presented too.