Reza Panjeshahin

Shiraz University of Medical Sciences | SUMS · Department of Pharmacology

Background Based on the anti-inflammatory and anti-oxidant properties of kaempferol and apigenin, we hypothesized that co-injection of these phytochemicals would increase the effectiveness of cell therapy in knee osteoarthritic rats. Methods Anterior cruciate ligament transection (ACLT) was used to induce osteoarthritis (OA). Animals were treated...

Background Drug side‐effects impose a lot of costs on health and treatment systems. Although the Ceftriaxone biliary or renal stone and sludge, lacks any symptom and it could go away spontaneously, but some cases require surgical intervention. Aims Examine the biliary or renal stone and sludge due to ceftriaxone in patients with different variants...

Background: The treatment of choice for hydatidosis as an important zoonotic disease is surgery. Different agents are injected into the cyst to prevent secondary hydatidosis. To avoid the side effects of such protoscolicidal agents, considering the high protoscolicidal effects of the garlic extract, we conducted the present study on protoscolices i...

Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in infertile rat model by stereological method. Thirty-two male rats were divided into four groups. Infertility was indu...

Background Hawthorn species decreases blood pressure and relaxes precontracted vessels. This study aimed at examining the antihypertensive effect and related mechanisms of hydroalcoholic extract of Crataegus azarolus subspecies aronia fruit in rats with renovascular hypertension. Methods Six groups of male Sprague-Dawley rats, each containing 6 to...

Background Cyclosporine A (CsA) is an immunosuppressant with therapeutic indications in various immunological diseases; however, its use is associated with chronic nephropathy. Oxidative stress has a crucial role in CsA-induced nephrotoxicity. The present study evaluates the protective effect of edaravone on CsA-induced chronic nephropathy and inve...

Background The induction of brain-derived neurotrophic factor (BDNF) expression in the hippocampus has shown to play a role in the beneficial effects of resveratrol (RSV) on the learning and memory. The BDNF gene has a complicated structure with eight 5’ noncoding exons (I-IXa), each of which can splice to a common coding exon (IX) to form a functi...

Hydroalcoholic (70°) extract of leaves of Myrtus communis has been shown to have antidiabetic effect in streptozotocin induced diabetic rats in our previous study. In this study, we intended to determine the components of the mentioned extract and identify the mechanism for its action. The leaves of Myrtus communis were extracted using petroleum et...

We aimed to compare the effects of oral ethanol (Eth) alone or combined with the phytoestrogen resveratrol (Rsv) on the expression of various brain-derived neurotrophic factor (BDNF) transcripts and the encoded protein pro-BDNF in the hippocampus of pregnant and embryonic rats. A low (0.25 g/kg body weight (BW)/day) dose of Eth produced an increase...

Resveratrol has beneficial effects on cardiovascular system. This study aimed at examining antidiabetic and antihypertensive effects of resveratrol in rats with simultaneous type 2 diabetes and renal hypertension. Eight groups (8-10 each) of male Spargue-Dawley rats, including a control, a diabetic (induced by streptozotocin and nicotinamide), a re...

This study aimed at examining the cardioprotective effects of resveratrol in rats with simultaneous type 2 diabetes and renal hypertension. Eight groups (8-10 each) of male Sprague-Dawley rats, including a control, a diabetic, a renal hypertensive, a sham, a simultaneously hypertensive-diabetic receiving vehicle, and 3 simultaneously hypertensive-d...

BACKGROUND Burns are still considered one of the most devastating conditions in emergency medicine affecting both genders and all age groups in developed and developing countries, resulting into physical and psychological scars and cause chronic disabilities. This study was performed to determine the healing effect of curcumin on burn wounds in rat...

Hepatic encephalopathy (HE) is a potentially reversible spectral neuropsychiatric state that complicates liver disease and negatively affects the prognosis. This study evaluated the effect of Curcuma longa in acute HE in rat and determined the behavioral, biochemical, and histological changes. Forty male Sprague Dawley rats were randomly divided in...

Background: Molecular imprinting is a method for synthesizing polymers with structure-selective adsorption properties with applications such as, selectivity binding, drug delivery systems and anti-bodies. The present study aims at optimizing the preparation of molecularly imprinted polymer (MIP) against l-phenylalanine, in order to increase phenyla...

Neurodegeneration is the pathophysiological basis for permanent neurological disabilities in multiple sclerosis (MS); thus neuroprotection is emerging as a therapeutic approach in MS research. Modulation of excitotoxicity by inhibition of NMDARs has been suggested for neuroprotection, but selective antagonisation of the NR2B subtype of these recept...

Polymer technology plays an influential role in biomedical sciences. Molecular imprinting is a technique for preparation of polymers with structure-selective adsorptive properties. High selectivities of these materials have nowadays advanced to the point that they are being utilized for several biomedical applications such as drug delivery. Phenylk...

Objective(s): The dopamine level of the nucleus accumbens changes during some stereotyped behaviors. To study dopamine level of the nucleus accumbens in intra infralimbic apomorphine-induced climbing, microdialysis probes were implanted into the nucleus accumbens shell of male Sprague Dawley rats weighting 275–400 g. Materials and Methods: The rat...

Background: In traditional medicine zingiber officinale used to regulate female menstural cycle and treat male infertility. Recent studies have suggested the possible role of ginger extract in improving the testicular damage of busulfan. Objective: The aim of this study was to evaluate the effects of zingiber officinale on the sperm parameters, tes...

Syzygium aromaticum was considered as an aphrodisiac agent; however, it may cause some side effects. The aim of this study was to examine the effects of Syzygium aromaticum extract on male reproductive system. To do this, forty male mice were divided into 5 groups: negative control was fed with vehicle, positive control was fed with aphridite and t...

Walnut is a medicinal plant that its different parts such as leaf, seed, root and green husk was shown to reduce blood glucose. In Iranian traditional medicine, septum of walnut shell (SWS) was recommended to reduce blood glucose. But this effect should be determined with scientific researches. Therefore, the aim of this study is to evaluate the ef...

We determined the effect of Elaeagnus angustifolia extract on chondrogenesis and osteogenesis in mouse embryo limb buds in vitro and in vivo. Limb bud mesenchyme from day 12.5 embryos were used for high-density micromass cultures. Water/alcohol extract was added to culture media at 10, 100, 1000 and 10000 μg/L. Cytotoxicity was tested with neutral...

In order to investigate the hepatoprotective effects of whey protein isolate against acute liver toxicity induced by dimethylnitrosamine (DMN), a randomized experimental study was conducted. Forty-eight Sprague Dawley rats were randomly divided into three groups. Groups A and B consumed a diet containing casein, and group C received a diet containi...

Resveratrol is a plant polyphenolic compound. Evidence indicates that resveratrol has beneficial effects against aging and neurodegenerative diseases. The goal of our study was in vivo examination of the effects of resveratrol on the abundance of mRNA encoding Brain Derived Neurotrophic Factor (BDNF) in the hippocampus of rat brain. Rats were admin...

Induction of hypothyroidism by thioamide drugs will cause adrenal gland atrophy and decrease in its hormones. To prevent side effect on the adrenal gland, brewer's yeast, a natural product rich in vitamins and minerals was used. Serological techniques were applied to measure the volume of adrenal gland. For this purpose, 48 Sprague-Dawley rats were...

Amini, M., Kherad, M., Mehrabani, D., Azarpira, N., Panjehshahin, M.R. and Tanideh, N. 2010. Effect of Plantago major on burn wound healing in rat. J. Appl. Anim. Res., 37: 53–56.To determine the effect of Plantago major on healing of burn wounds, 100 male Sprague-Dawley 180–220 g rats were randomly divided into 4 groups. Groups of A, B and C each...

Caraway is one of the medicinal plants used for digestion problems, however, it has been reported that the long term use of this plant in large doses might cause hyperthyroidism symptoms such as leanness, hair loss, severe sweating and tremulousness. Therefore, the aim of this study was to evaluate the effect of hydroalcoholic extract of caraway on...

To determine the healing effect of Teucrium polium (T. polium) in indomethacin-induced gastric ulcer in rats. In the fall of 2007, 250 Sprague-Dawley rats provided by the Shiraz University Laboratory Animal Center were divided into 4 equal groups including control (70 rats), and 3 experimental groups (60 rats each), and each group received differen...

While suicide by burns is a relatively uncommon form of suicide in developed countries, it is one of the most common methods of suicide in the Middle East region including Iran. To describe epidemiologic characteristics of suicidal behaviors by burns in the province of Fars, Iran. A prospective population-based study of all suicidal behaviors by bu...

This study was conducted to assess the protoscolicidal effect of garlic (Allium sativum) extract on protoscoleces of hydatid cysts in vitro. Viability of protoscoleces was determined, as the percent of viable protoscoleces to the total protoscoleces in 1 ml of the hydatid fluid, using eosin stain method. Four types of garlic extracts including: aqu...

Kheradpezhouh, E., Miri, R., Noorafshan, A., Panjehshahin, M.R. and Mehrabani, D. 2008. A new method of brain staining with triphenyltetrazolium chloride to determine the infarct size in rats. J. Appl. Anim. Res., 33: 149–152.To show the accuracy of 2, 3, 5-Triphenyltetrazolium chloride (TTC) staining method, in a case-control study, 10 rats were d...

This study investigated the effect of a selective A(1)-adenosine receptor (A(1)-AR) antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), on the renal dysfunction and histological damage induced by ischaemia/reperfusion at an early stage. Pentobarbital anaesthetised rats were prepared for measuring renal functional variables. Ischaemia was induce...

Background: Metronidazole has been reported to reduce postoperative anaerobic infections following surgical procedures. Because of high cost and poor availability of intravenous metronidazole compared with that of oral preparation, we decided to measure the serum level of metronidazole after oral administration in patients during postoperative ileu...

Adrenomedullin (AM) and calcitonin gene-related peptide (CGRP) are structurally related, interact with each others receptors and show overlapping biological activities. Immunoreactivity (IR) and mRNAs along with binding sites for both CGRP and adrenomedullin have been shown in the rat spinal cord. CGRP mediates the transmission of nociceptive infor...

Vojdani, Z., Khozani, T.T., Dehghani, F., Panjehshahin, M.R., Habibi, A. and Mehrabani, D. 2007. Effect of hydro-alcoholic extract of soybean on embryonic growth and ossification indices in mouse. J. Appl. Anim. Res., 31: 117?120. To evaluate the effects of soybean hydro-alchoholic extraction on growth and ossification indices, fifty female mice we...

Hormonally active agents are widespread in the environment and exist in the human diet. There is insufficient knowledge regarding the influence of phytochemicals on the pathophysiology of the male reproductive system. Kiwi is a rich source of phytochemicals such as flavones (with estrogenic activity), serotonin and vitamin C. These components can i...

Background: There is a rapidly growing trend in the consumption of herbal remedies in the developing countries. The Phoenix dactylifera date palm pollen (DPP) is used in the traditional medicine for male infertility. The aim of this study was to determine the effects of orally administered DPP on the reproductive system of adult male rats. Methods:...

The partial least squares regression method has been applied for simultaneous spectrophotometric determination of harmine, harmane, harmalol and harmaline in Peganum harmala L. (Zygophyllaceae) seeds. The effect of pH was optimized employing multivariate definition of selectivity and sensitivity and best results were obtained in basic media (pH>9)....

There is an increasing tendency for traditional medicine in the world. Many people prefer to take herbal products instead of chemical medicines. However, over consumption of herbal medicines has led to many unpredictable side effects.One of these traditional medicines is Citrullus colocynthis, which is used by diabetic patients as an hypoglycemic a...

Dehghani, F., Panjehshahin, M.R., Mirzaee, Z. and Mehrabani, D. 2005. Effect of Foeniculum vulgare organic extract on blood sex hormones and reproductive tissues of male rats. J. Appl. Anim. Res., 27: 17–20.To determine the effect of F. vulgare on reproductive system in male rats, forty male Sprague-Drawly rats were randomly placed in 4 equal group...

Adrenomedullin (AM) immunoreactivity and mRNA, in addition to a large number of specific AM-binding sites, exist in the rat spinal cord. However, no phenotype has been reported for AM in the spinal cord. Here, expression of c-fos in response to intrathecal (i.t.) administration of AM, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin ge...

Background: Group A beta hemolytic streptococci (GABHS) is a potentially pathogenic bacteria which causes various infections among children and adults. For over 60 years, penicillin has been used as the drug of choice for treatment of infections caused by this bacterium. Yet, there is no published article on the resistance of this bacterium to peni...

Hyperlipidemia is an important modifiable risk factor of coronary heart diseases. So far, several studies, have indicated the beneficial effects of nuts on plasma lipid profile. Previously, in a pilot study the authors have shown that administration of 20 g/day of Persian walnut (Juglans regia L.) for 8 weeks could decrease plasma triglyceride (TG)...

Panjehshahin, M.R., Owji, A.A., Mehrabani, D., Mirzaee, M. and Vasei, M. 2003. Effect of curcumin on cholesterol gall-stone induction in rats. J. Appl. Anim. Res., 23: 75–80.To study the effects of curcumin on the formation of cholesterol gallstones, 30 female rats were divided into three equal groups. Diet of rats in lithogenic group was supplemen...

High concentrations of glucagon-like peptide-1 (7-36) amide (GLP-1) and its specific receptor (GLP-1R) have been found in the rat hypothalamus. In this study the actions of GLP-1 and its related peptides, exendin-4 (GLP-1R agonist), exendin (9-39) (GLP-1R antagonist) and GLP-1 (9-36) amide (the major GLP-1 metabolite) on levels of serotonin (5-HT),...

Background: The bioavailability of metronidazole after oral ingestion, in ordinary conditions, is quite similar to intravenous administration of the drug. However, the degree of its absorption is not known in the early periods after laparotomy. Objective: To determine plasma levels of metronidazole in pediatric patients following elective abdominal...

Childhood burn injuries are a major environmental agent responsible for significant morbidity and mortality in Iran. Thus, the objective of this study was to analyze the epidemiology and current causative factors of hospitalized burn injuries among the approximately 1.6 million children between the ages of birth through 15 years residing in the Far...

Background: Esophageal carcinoma is one of the most lethal gas- trointestinal (GI) malignant tumors worldwide. The disease is known to have a high incidence in northern Iran; however, its condi- tion in the South is not much clear.

Research report Effects of intracerebroventricular injection of glucagon like peptide-1 and its related peptides on serotonin metabolism and on levels of amino acids in the rat hypothalamus a , a a b b Abstract High concentrations of glucagon-like peptide-1 (7-36) amide (GLP-1) and its specific receptor (GLP-1R) have been found in the rat hypothala...

Research report Effects of intracerebroventricular injection of glucagon like peptide-1 and its related peptides on serotonin metabolism and on levels of amino acids in the rat hypothalamus a , a a b b Abstract High concentrations of glucagon-like peptide-1 (7-36) amide (GLP-1) and its specific receptor (GLP-1R) have been found in the rat hypothala...

Burn injuries still produce a significant morbidity and mortality in Iran. This study was carried out to analyze the epidemiology, mortality, and current etiological factors of 2043 burn patients who were admitted to the burn centers in the Fars province during 4 years (1994-1998). There were two burn centers in the Fars province serving 3817036 pe...

Old World cutaneous leishmaniasis has many different clinical presentations. A rare and unusual presentation of cutaneous leishmaniasis is the erysipeloid type. This clinical form is not only unusual in its clinical features but also in the specific category of patients it seems to afflict. In this report 5 Iranian patients, predominantly females,...

A double-blind randomized clinical trial was undertaken to compare the efficacy of meglumine antimoniate solution (Glucantime), administered intramuscularly, with a topical herbal extract consisting of a pure extract mixture of Althaea rosa, Althaea officinalis, and members of the families Leguminosae, Faliaceae, Malvaceae, and Lythraceae (named Z-...

Background: It has been shown that adenosine mediates hemodynamic changes in some forms of acute renal failure (ARF) in rats. Objective: To evaluate the degree of nephrotoxicity in uranyl nitrate (UN)-induced renal failure in rats with and without 1,3-dipropyl 8-cyclopentylxanthine (CPX). Methods: Acute renal failure was induced by injection of sin...

Nephrotoxicity of cyclosporin A(CsA) is the major side effect of this potent immunosuppressive drug. In order to evaluate the involvement of serotonin in CsA nephrotoxicity, rats were treated with different doses (1,10,30 and 100 mg/kg/day) of CsA on four consecutive days. Elevated serum urea and creatinine levels was accompanied by structural evid...

The effect of glycerol-induced acute renal failure (ARF) on serotonin (STN) was studied in rats. The levels of STN in plasma and platelets (PT) were measured by high performance liguid chromatography (HPLC). The concentration of STN in platelets (4483 ± 875 ng/109 PT) and plasma (83.50 ± 9.75 ng/ml plasma) were higher than that of healthy controls...

In the rat, treatment with the alkylxanthine 8-cyclopentyl-1,3-dipropylxanthine (CPX) at a dose of 0.1 mg kg-1 antagonizes adenosine-induced falls in renal blood flow and reduces the severity of glycerol-induced acute renal failure. Treatment of glycerol-injected rats with 0.03 mg kg-1 of CPX resulted in no significant improvements in a range of in...

The effect of the selective A1-adenosine receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (CPX, 0.1 mg kg-1 i.v.) administered twice daily to rats has been assessed on the development of renal dysfunction induced by four daily injections of cyclosporin (60 mg kg-1 i.p.). The series of cyclosporin injections resulted in a polyuria accompanied...

The sites to which valproic acid and its main unsaturated metabolites (2-en-2-propyl pentanoic acid and 4-en-2-propyl pentanoic acid) bind to on human albumin were investigated by (1) measuring their ability to displace the fluorescent probes warfarin and dansylsarcosine and (2) by assessing the extent to which they inhibited the hydrolysis of 4-ni...

Background: Quantitative changes of histopathological structures are important in the process of improvement or regression of diseases. The aim of this study was to stereologically assess kidney volume (cortex and medulla) in glycerol-induced acute renal failure (ARF). Materials and Methods: Twenty-four Sprague Dawley rats were selected and randoml...

Abstracts Objective(s) While the nucleus accumbens ,and the striatum have ,received ,much ,attention regarding their roles in stereotyped behaviors, the role of the medial prefrontal cortex (mPFC) has not beeninvestigated to the same degree. Few studies have ,reported the role of the mPFC ,in dopaminergic ,induction of locomotor hyperactivity. The...

The probability of cardiovascular diseases in diabetic patients increases due to hyperlipidemia. Raphanus sativus is a traditional plant which is used to lower plasma lipid. The aim of this study was to evaluate the effect of aqueous extract of Raphanus Sativus on plasma glucose, triglyceride, and cholesterol in diabetic rats. For this purpose, 30...