
Remedios Castro-MejíasUniversidad de Cádiz | UCA · Department of Analytical Chemistry
Remedios Castro-Mejías
Professor
About
94
Publications
24,110
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,527
Citations
Introduction
Additional affiliations
February 1995 - present
Publications
Publications (94)
This work aims to determine the effect on the aromatic profile and phenolic content of red wine vinegars produced by surface culture at two different temperatures (30 °C and 37 °C) and using different inocula of acetic acid bacteria (Acetobacter malorum, Gluconobacter oxydans and a mixture of bacteria isolated from a Sherry vinegar). Fifty-seven vo...
Due to global warming and the effects associated with it, the wine industry is facing important challenges during the winemaking process and the production of high-quality wines. In this study, mistelas and naturally sweet wines were produced with the ‘Pedro Ximénez’ grapevine cultivar, overripened by sun drying and fermented with and without the p...
BACKGROUND
In the present study, the ageing process of Sherry vinegar in used (seasoned) or new casks made of chestnut, American oak, Spanish oak or French oak wood has been investigated, considering that no research has investigated whether this seasoning has a definite influence on the final composition of the aged beverage. The polyphenolic and...
Prickly pear (Opuntia ficus-indica (L). Mill.) is a plant that belongs to the Cactaceae family and can grow in arid and semi-arid climates. This fruit is considered a good source of nutrients, antioxidant constituents and health-promoting substances, such as betalains and polyphenols. One of the ways in which the communities in the cultivation area...
The development of new non-dairy probiotic foods is interesting, given lactose intolerance, milk allergies, and the growing trend of vegetarianism. In this paper, beer has been used as a probiotic delivery matrix, using Saccharomyces boulardii as an alternative to conventional brewer’s yeast. The strain was able to grow in worts prepared with hops...
BACKGROUND
This study focused on the valorization of prickly pear (PP) fruit (Opuntia ficus‐indica) into vinegar by spontaneous surface fermentation on different starting matrices (with/without the addition of sucrose and with/without PP peel in the raw material). Different parameters were monitored during the fermentation process in terms of their...
The brewing industry has experienced a significant boom in recent years through the emergence of, on the one hand, craft breweries that produce beers with unique organoleptic characteristics, and, on the other hand, the brewing of a significant number of beers using hybridized or genetically modified microorganisms with the aim of improving both th...
Brandy is a unique alcoholic beverage obtained from wine distillates. Numerous studies
have been published on its physicochemical traits and the effect of certain elaboration variables on them, but not many studies have been carried out from a sensory point of view or that have followed standardized methods applicable to this discipline. This study...
In the past few years, there has been a growing demand by consumers for more complex beers with distinctive organoleptic profiles. The yeast, raw material (barley or other cereals), hops, and water used add to the major processing stages involved in the brewing process, including malting, mashing, boiling, fermentation, and aging, to significantly...
Stir bar sorptive extraction (SBSE) is a rapid, sensitive, precise, and environmentally friendly extraction technique that, coupled to gas chromatography–mass spectrometry detection (GC–MS), enables the simple determination of volatile organic compounds in liquid samples. The present protocol describes the procedure for the determination of volatil...
Solid-phase microextraction (SPME) is an easy, sensitive, and environmentally friendly technique that has been employed, coupled to gas chromatography or liquid chromatography, to determine a huge amount of analytes with different volatilities. The present work describes the procedure to follow in order to determine volatile compounds in vinegar by...
The production of vinegar on an industrial scale from different raw materials is subject to constraints, notably the low tolerance of acetic acid bacteria (AAB) to high temperatures and high ethanol concentrations. In this study, we used 25 samples of different fruits from seven Moroccan biotopes with arid and semi-arid environmental conditions as...
BACKGROUND
In several countries, the cactus plant (Opuntia ficus‐indica (L). Mill.) has received renewed attention because of its ecological, socio‐economic and environmental role. In this study, prickly pear vinegar was produced employing two types of acetification processes: surface and submerged culture. Both acetification processes were perform...
Beer is a biotechnologically produced beverage that results from the anaerobic fermentation of the sugars contained in cereals such as barley or wheat. Such fermentation is mainly carried out by Saccharomyces cerevisiae yeasts, but other microorganisms — other yeast species or bacteria — may be involved in the process. Depending on such microbiota...
Prickly pear (Opuntia ficus-indica (L). Mill.) vinegar has been produced by submerged culture using scale laboratory fermenters at three acetification temperatures (30 °C, 37 °C, and 40 °C). Pure thermotolerant Acetic Acid Bacteria (AAB) which had been previously submitted to molecular identification by PCR–RFLP of the 16S-23S rDNA regions and 16S-...
The accelerated ageing of a Tempranillo red wine has been studied at pilot scale through the combined application of ultrasound, micro-oxygenation and different oak wood type chips (American, French and Spanish). The phenolic and volatile content of the aged samples and their sensory profiles have been determined. The wine samples that had been age...
This work intends to determine the effect on the aroma profile, phenolic content and antioxidant activity of prickly pear vinegars produced by the surface culture at two different fermentation temperatures and using different acetic acid bacteria (AAB) inocula. Prickly pear wine was fermented at two temperature levels (30 and 37 ºC) by using bacter...
Food industry has been recently aiming among other things toward the production of functional foods. Probiotics fall into this category. Although dairy food matrices have traditionally been used for the elaboration of them, in recent years, a number of allergic or abnormal reactions to some of the ingredients that are normally found in dairy matric...
The influence of the hopping method (boiling stage hopping or dry hopping), the fermentation temperature (12 and 18 °C), and the yeast strain (five different yeasts) on the physical-chemical characteristics (bitterness, color, alcohol content), the phenolic content, the volatile compounds, as well as the sensory profile of beers, has been studied....
Both aroma and taste are crucial quality criteria for food products, having a great influence on our consumption behaviour [...]
Ageing in wood is one of the most important stages among oenological products elaboration procedures since it plays an important role with regard to the final product’s organoleptic characteristics and quality standards. Unfortunately, it is a slow process and, therefore, a rather costly one. New technologies are starting to be applied to this agei...
Aroma is one of the main responsible for the acceptance of oenological products such as wine, vinegar and derived products [...]
The production of fruit vinegars as a way of making use of fruit by-products is an option widely used by the food industry, since surplus or second quality fruit can be used without compromising the quality of the final product. The acetic nature of vinegars and its subsequent impact on the organoleptic properties of the final product allows almost...
This work proposes the elaboration of a product based on the maceration of Sherry Vinegar together with pineapple in order to extract certain volatile compounds that can be found in pineapples, giving a final product with new organoleptic properties and increased polyphenolic content. Maceration trials were carried out with the application of micro...
Jerez (Sherry) is a well-known wine-producing region located in southern Spain, where world-renowned oenological products such as wines, vinegars, and brandies are produced. There are several factors that provide characteristic physical, chemical, and sensory properties to the oe-nological products obtained in this Sherry region: the climate in the...
In the present work, we have studied the effect that some extraction techniques, such as ultrasound or microwaves, produce on the aroma of red wines, when applied to processes of extractive nature, such as pre-fermentative maceration or ageing with oak chips (Spanish oak – Quercus pyrenaica and French oak – Quercus robur). The volatile profile has...
In the present work, the polyphenolic profile of a complex matrix such as Amontillado
sherry has been processed by means of high-speed counter-current chromatography (HSCCC) and characterized by HPLC-DAD-MS. An Amberlite XAD-7 column was used to obtain the wine extract, and three different biphasic solvent systems were applied for HSCCC separation:...
BACKGROUND
In the present work we propose the use of accelerating energies (microwaves and ultrasounds) to the maceration process of sherry vinegar with citrus fruit peels (orange and lemon). For the application of microwaves, an experimental design has been developed in order to optimize the maceration conditions. To evaluate the effect of these e...
BACKGROUND
Various physicochemical parameters, the polyphenolic content, the volatile fraction as well as the sensory profile of beers were studied using five different yeast strains (three top‐fermentation and two bottom‐fermentation) and two different fermentation temperatures (12 and 18 °C) for each of them.
RESULTS
The results indicated that a...
BACKGROUND
The effect of different cooking methods (boiling, vacuum and steamed cooking) on the volatile compound content and sensory properties of sea lettuce (Ulva rigida) seaweed was assessed. Sea lettuce was cooked at three different temperatures (50, 70 and 100 °C) for three different lengths of time (5, 10 and 15 min). Various statistical tec...
Thirty one samples from different macroalgae species have been studied to determine the influence of several parameters such as the harvesting season, the geographical origin, the species or a pretreatment procedure on their volatile composition. A Multiple Head Space Sorptive Extraction methodology coupled to Gas Chromatography with Mass Spectrome...
In the present work, the accelerated ageing process of sherry vinegar has been studied at pilot scale by means of the joint application of ultrasound, micro-oxygenation and wood chips (American oak, French oak and Spanish oak). The CIELab parameters have been studied as well as the polyphenolic and volatile content of the aged vinegar samples. Vine...
In this study, a climate chamber, as an alternative method, has been used to dry raisins and the sensory profiles of the sweet sherry wines obtained have been evaluated. Other important factors, namely grape variety, vintage, vinification conditions, as well as the ageing method and its length of time, have also been considered. When heavy rainfall...
Accelerated maceration experiments of orange peels in Sherry vinegar employing ultrasounds (US) have been carried out. Their effect on the vinegaŕs volatile composition as well as on its olfactometric and sensory characteristics have been evaluated. The optimal conditions for the ultrasounds were determined as follows:; sonication power 550 W/L; pu...
A headspace sorptive extraction method coupled with gas chromatography–mass spectrometry (HSSE–GC–MS) was developed for the determination of 37 volatile compounds in beer. After optimization of the extraction conditions, the best conditions for the analysis were stirring at 1000 rpm for 180 min, using an 8-mL sample with 25% NaCl. The analytical me...
BACKGROUND
The present study investigated the volatile composition of a Pedro Ximénez sweet wine that had been aged in barrels made of different types of wood (Spanish oak, French oak, American oak and chestnut) and subjected to different degrees of toasting (medium toasting and intense toasting). The analyses were carried out using stir bar sorpti...
The present study has consisted on the monitoring of Sherry vinegar during a whole year ageing in American oak, French oak, Spanish oak and chestnut barrels in order to determine the suitability of alternative wood types other than American oak for the ageing of this type of vinegar. Thirty-two volatile compounds were quantified, CIELab parameters...
Thirty one Jerez vinegar samples and 33 Huelva vinegar samples were analyzed for polyphenolic and volatile compound content in order to characterize them and attempt to differentiate them. Sixteen polyphenolic compounds were quantified by means of ultraperformance liquid chromatography method with diode array detection (UPLC–DAD), and 37 volatile c...
BACKGROUND
Edible seaweeds are one of the most important resources that currently exist for their multiple uses and applications. Like other food types, during their storage there are significant changes in sensory characteristics associated with loss of freshness and with production and/or degradation of their volatile compounds. In this study, mu...
A research on the production of lemon vinegar directly from lemon juice was conducted. For the alcoholic fermentation, two different strains of Saccharomyces cerevisiae, as well as two types of cultures (submerged culture and yeasts immobilized in alginate beads) were used. A microbiological control was also implemented during this stage. Acetic fe...
Odour-active compounds in three traditional balsamic vinegars from Modena (TB) and seven balsamic vinegars from Modena (PGI) were determined by gas chromatography-olfactometry (GC-O) using frequency of detection methodology (modified frequency, MF, %). The main odour compounds (mean MF > 60%) were 2,3-butanedione (75%), acetic acid (70%), furan-2-c...
A novel extraction technique is proposed in which the Multiple Headspace Extraction (MHE) approach is used in conjunction with Headspace Sorptive Extraction (HSSE) and Gas Chromatography-Mass Spectrometry (GC-MS) detection. The extraction method was developed to determine volatile compounds in macroalgae. Optimization of the extraction parameters w...
Background:
There is growing interest in the beneficial health effects of certain fruits, such as pomegranate, and their by-products, like vinegar. Vinegars contain antioxidant compounds such as polyphenols, which can scavenge free radicals in the body. In this study, the antioxidant properties (ABTS and DPPH scavenging capacities) and global poly...
Background:
The two main acetification methodologies generally employed in the production of vinegar (surface and submerged cultures) were studied and compared for the production of orange vinegar. Polyphenols (UPLC/DAD) and volatiles compounds (SBSE-GC/MS) were considered as the main variables in the comparative study. Sensory characteristics of...
Fresh tomato and tomato products are widely consumed products. Their beneficial properties are not only present in fresh products but also in fermented ones. Therefore, the production of fresh tomato derivatives could be a good alternative to get a higher diversification and to make a good use of possible overproductions. Tomato vinegar from tomato...
Several experiments were conducted to developed orange based vinegar by surface culture. The addition of sugar (sucrose and concentrated must) and the presence/absence of peel in the raw material (squeezed juice, peeled orange, non-peeled orange plus squeezed juice) have been studied during the development of the final product. Polyphenolic and vol...
A stir bar sorptive extraction method for the determination of volatile compounds in orange juices was developed. The extraction variables were optimized using a reduced two-level factorial screening design (25-1), and the most suitable analytical conditions for the extraction of the studied compounds were: sample volume 10 mL, extraction time 60 m...
Sweet Sherry wines from Pedro Ximénez and Muscat sun and chamber-dried grapes during vintages 2009 and 2010 were aged in American oak wood for 12 months. Their volatile content was periodically analyzed using SBSE-GC-MS. Cluster analysis and principal component analysis demonstrated that the volatile compounds considered can be used to detect grape...
Head space sorptive extraction (HSSE) has been evaluated for the analysis of volatile compounds from oak wood in wines. The extraction conditions have been optimized using a two-level factorial design. After optimization, the final extraction conditions were: PDMS stir bars (20 mm length × 0.5 mm film thickness), sample volume of 5 mL, extraction t...
Background:
Stir bar sorptive extraction (SBSE) has been employed for the analysis of aroma compounds directly from Spanish and Italian grape extracts. The extraction conditions have been optimised using a statistical approach, obtaining results that improve the current state of the art.
Results:
The proposed analytical conditions were: sample v...
Dietary fiber derived from citrus fruits was added to vinegar. Different sources and quantities of fiber and storage conditions have been scrutinized. Formulated vinegars were evaluated on the basis of their phenolic profile, volatile composition and sensory analysis. The addition of citrus fiber enhanced the phenolic and volatile profile of the re...
The current tendency in the extraction procedures is to develop accurate, easy-to-automate, and sensitive methodologies which can reduce sample handling. Stir bar sorpive extraction (SBSE) is a recently developed technique in which a stir bar coated of polydimethylsiloxane (PDMS) is employed to extract analytes directly from minimally treated matri...
Background and AimsEthyl carbamate (EC) is a known carcinogen that has been found in wines. The aims of this study were: (i) to study the effect of fermentation under different enological conditions on the EC concentration in sweet Sherry wines made from the two grape cultivars, Muscat and Pedro Ximénez, during three consecutive vintages (2008–2010...
A fast, simple and reliable analytical method for the determination of medium-level volatile thiols in wines is presented. Stir bar sorptive extraction using ethylene glycol-silicone coated stir bars has been used in combination with thermal desorption gas chromatography with mass spectrometry for the analysis of 4-mercapto-4-methylpentan-2-one, 2-...
Several experiments of aging in oak wood and stainless steel of sweet Sherry wines obtained from grapes cv. Muscat fermented under different enological conditions have been carried out. These wines were periodically sampled and their volatile content was analyzed using SBSE–GC–MS. A sensory study was also carried out. Data were submitted to differe...
Background:
Nineteen commercially available aromatised vinegars, which were representative of this type of product, were tested to ascertain their phenolic and volatile composition and antioxidant activity. The aromatised vinegars came from different raw materials such as fruits, spices, herbs and vegetables. The antioxidant activity was determine...
Background:
Despite the long history of sherry wine vinegar, new alternatives of consumption are being developed, with the aim of diversifying its market. Several new acetic-based fruit juices have been developed by optimising the amount of sherry wine vinegar added to different fruit juices: apple, peach, orange and pineapple. Once the concentrat...
Several experiments of maceration of a Sherry wine vinegar with different fruits (orange, lemon, strawberry, grapefruit, and lime) have been carried out. After a preliminary study (only peel, no heating and 7 days as maximum time of maceration), parameters such as volatile compounds and sensory characteristics were determined in Sherry wine vinegar...
A stir bar sorptive extraction gas chromatography–mass spectrometry (SBSE–GC–MS) method has been used for the characterization of the volatile composition of 26 high quality vinegars of three different protected geographical indications (traditional balsamic vinegar of Modena, balsamic vinegar of Modena, and Sherry vinegar). SBSE technique provided...
Aroma compounds of 48 Sherry brandies have been identified and quantified by the stir bar sorptive extraction method coupled to gas chromatography-mass spectrometry (SBSE-GC/MS). Analysis of variance and multivariate analysis techniques have been used to classify these brandy samples according to the commercial category (Solera brandy, Solera Reser...
Several experiments of maceration of a sherry wine vinegar with different fruits (orange, lemon, strawberry, grapefruit, and lime) have been carried out. After optimization (only peel, no heating and seven days as maximum time of maceration), parameters such as polyphenolic content, superoxide anion scavenging ability (related to antioxidant activi...
Sherry brandy (Jerez, SW, Spain) is a high quality distilled beverage derived from wine. Its perceived quality depends, inter alia, on hundreds of flavour compounds. A Stir Bar Sorptive Extraction (SBSE) method coupled to gas chromatography-mass spectrometry has been developed for the analysis of volatile compounds in Sherry brandy. The optimizatio...
FT-IR calibrations have been made to estimate the aromatic compounds present in vinegars during the acetic fermentation process. A change in the concentration of the major species takes place in this process; ethanol drops from values of around 12% to <1%, while acetic acid increases from values of <0.02% to >10%. To perform these FT-IR calibration...
Due to the diversity of vinegars on the market and the increase in demand, it is considered necessary to investigate and establish criteria for classifying them in order to obtain more information concerning their real origin. New spectroscopic techniques such us mid-infrared spectroscopy with Fourier transform (FT-IR) are capable of providing info...
A study of accelerated aging on an industrial scale, employing both microoxygenation and oak chips, of a Sherry wine vinegar
has been carried out. The effect of these two factors, microoxygenation and oak chips, on polyphenolic and volatile compounds,
and sensorial parameters has been evaluated. The dose of oxygen applied to the vinegar and the use...
In the present work, the monitoring of the evolution of the different phytosanitary products employed in the production of a Sherry wine vinegar has been carried out. The study covers the complete process, from the grape ripening to the vinegar fermentation. For the liquid sample analysis, a method based on SBSE (stir bar sorptive extraction) coupl...
Aroma compounds are most closely associated with the volatile fraction of foods. Common analytical separation procedures employed
to analyse volatile compounds need, even today, that prior to GC analysis of an aroma or a fragrance, these compounds be concentrated
or/and isolated from the non-volatile matrix. This step constitutes a problem that has...
The characterization of the volatile composition that could have relevance on the organoleptic profile of Andalusian sweet
wines obtained from Muscat and Pedro Ximenez grapes has been carried out. Twenty commercial samples, ten from Pedro Ximenez
grapes and ten for Muscat grapes, have been studied. Various different chemometric tools have been appl...
Stir bar sorptive extraction was evaluated for analysing volatiles in vinegar. The procedure developed shows detection and quantitation limits, and linear ranges adequate for analysing this type of compounds. The accuracy obtained was close to 100%, with repeatability values lower than 13%. The extraction efficiency is inversely affected by the ace...
Stir bar sorptive extraction (SBSE) has been evaluated for analysing pesticides in vinegar. The extraction analytical conditions have been optimised using a two-level factorial design expanded further to a central composite design. After optimization, the proposed analytical conditions are: sample volume 40 mL, sampling time 150 min, and stirring s...