Rein Aasland

Rein Aasland
University of Oslo · Department of Biosciences

Dr. scient.

About

99
Publications
6,367
Reads
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8,577
Citations
Citations since 2016
12 Research Items
2024 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
Additional affiliations
May 2010 - January 2011
National Institute for Medical Research, London
Position
  • Visiting professor (sabbatical)
January 2002 - December 2011
Uni Research (Bergen)
Position
  • Senior Researcher
Description
  • part time affiliation
June 2001 - June 2002
Ludwig-Maximilians-University of Munich
Position
  • Visiting professor (sabbatical)
Education
January 1985 - December 1989
University of Bergen
Field of study
  • Molecular Cancer Research

Publications

Publications (99)
Article
Full-text available
The CW domain binds to histone tail modifications found in different protein families involved in epigenetic regulation and chromatin remodeling. CW domains recognize the methylation state of the fourth lysine on histone 3 and could, therefore, be viewed as a reader of epigenetic information. The specificity toward different methylation states such...
Preprint
Full-text available
The CW domain binds to histone-tail modifications found in different protein families involved in epigenetic regulation and chromatin remodelling. CW domains recognize the methylation state of the fourth lysine on histone 3, and as such could be viewed as a reader of epigentic information. The specificity towards different methylation states such a...
Article
Chromatin post-translational modifications are thought to be important for epigenetic effects on gene expression. Methylation of histone N-terminal tail lysine residues constitutes one of many such modifications, executed by families of Histone Lysine Methyltransferase (HKMTase). One such protein is ASHH2 from the flowering plant Arabidopsis thalia...
Preprint
Full-text available
H3K27ac is associated with regulatory active enhancers, but its exact role in enhancer function remains elusive. Using mass spectrometry-based interaction proteomics, we identified the Super Elongation Complex (SEC) and GBAF, a non-canonical GLTSCR1L- and BRD9-containing SWI/SNF chromatin remodeling complex, to be major interactors of H3K27ac. We s...
Article
Full-text available
The ASHH2 CW domain is responsible for recognizing the methylation state at lysine 4 of histone 3 N-terminal tails and implicated in the recruitment of the ASHH2 methyltransferase enzyme correctly to the histones. The ASHH2 CW domain binds H3 lysine motifs that can be either mono-, di-, or tri-methylated [ARTK(meX)QTAR, where X denotes the number o...
Article
Full-text available
An approach to assay proteolytic activity in vivo by altering the subcellular localization of a labelled substrate was demonstrated. The assay included a protein shuttling between different cellular compartments and a site-specific recombinant protease. The shuttle protein used was the human immunodeficiency virus type 1 (HIV-1) Rev protein tandeml...
Article
Enterotoxigenic Escherichia coli (ETEC) are an important cause of diarrheal disease and death in children under five years of age. ETEC that express the heat-stable toxin (ST), with or without the heat-labile toxin, are among the four most important diarrhea-causing pathogens. This makes the ST toxin an attractive target for an ETEC vaccine. An ST...
Article
Full-text available
Enterotoxigenic Escherichia coli (ETEC) expressing the heat-stable toxin (ST; variants STh and STp) is among the five most important enteric pathogens in young children living in low-and middle income countries. ST mediates diarrheal disease through activation of the guanylate cyclase C (GC-C) receptor and is an attractive vaccine target with the p...
Article
Sirtuin-2 (SIRT2), the cytoplasmic member of the sirtuin family, has been implicated in the deacetylation of nuclear proteins. Although the enzyme has been reported to be located to the nucleus during G2/M phase, its spectrum of targets suggests functions in the nucleus throughout the cell cycle. While a nucleocytoplasmic shuttling mechanism has be...
Article
Full-text available
Post-translational modifications of the N-terminal histone tails, including lysine methylation, have key roles in regulation of chromatin and gene expression. A number of protein modules have been identified that recognize differentially modified histone tails and provide their proteins with the capacity to sense such modifications. Here, we identi...
Article
Full-text available
The heterotrimeric AMP-activated protein kinase (AMPK) has a key role in regulating cellular energy metabolism; in response to a fall in intracellular ATP levels it activates energy-producing pathways and inhibits energy-consuming processes. AMPK has been implicated in a number of diseases related to energy metabolism including type 2 diabetes, obe...
Article
Full-text available
Genomes of animals as different as sponges and humans show conservation of global architecture. Here we show that multiple genomic features including transposon diversity, developmental gene repertoire, physical gene order, and intron-exon organization are shattered in the tunicate Oikopleura, belonging to the sister group of vertebrates and retain...
Article
The phenotypes of different cell types are governed by their differential gene expression programmes, which are prominently influenced by epigenetic gene regulation featuring heritable chromatin states. Different epigenetic states are associated with distinctive patterns of post-translational modifications of the histone tails, which in turn influe...
Article
Enterotoxigenic Escherichia coli (ETEC) is responsible for 280 million to 400 million episodes of diarrhea and about 380,000 deaths annually. Epidemiological data suggest that ETEC strains which secrete heat-stable toxin (ST), alone or in combination with heat-labile toxin (LT), induce the most severe disease among children in developing countries....
Data
Further analyses of euchromatic H2A.Z ChIP. (A) To validate the ChIP-chip, samples were amplified by semi-quantitative PCR using primers to IGR and ORF regions according to the scheme for the gene SPAC12G12.01c, which is also shown in Figure 2B. The “beads” control demonstrates low background binding in an IP carried out without antibody. The “IN”...
Data
The inner centromeres of Chromosomes 2 and 3 also acquire H2A.Z and increased H3 levels in the absence of Swr1 or Msc1. (A) Chromosome 2 H2A.Z-myc ChIP. (B) The corresponding H3 ChIP. (C) Chromosome 3 H2A.Z-myc ChIP. (D) The corresponding H3 ChIP. The structural features are labeled below the panels. (4.97 MB EPS)
Data
H2A.Z ChIP on the sub-telomeres of all three chromosomes. ChIP-chip binding profiles for H2A.Z-myc at 180kb regions at both ends of chromosome 1 (A, B), 2 (C, D), and 3 (E, F) in WT, msc1Δ, and swr1Δ. Open reading frames are represented by black boxes and LTR retrotransposon elements by orange boxes. The dotted lines demonstrate the approximate tra...
Data
H2A.Z is found in two isoforms, distinguished by two N-terminal methionines, and can be acetylated on all four lysines of the N-terminal tail. (A) Listed are H2A.Z peptides as detected by LC-MS/MS analysis. The observed and predicted masses are presented, and the difference between these values (delta) given in parts per million (ppm), as are the n...
Data
Acetylation of H2A.Z-associated H2B is not strongly affected by the loss of either Swr1 or Msc1. Relative quantification of H2A.Z-associated H2B 1–18 acetylation from WT, msc1Δ, and swr1Δ, plus global H2B 1–18 acetylation levels in WT. H2B can be acetylated on three lysine residues of the N-terminal tail (K5, K10 and K15) and the N-terminus, and is...
Data
Full-text available
Msc1 is a conserved JmjC-domain containing Jarid family member. Multiple amino acid alignment of H. sapiens Jarid1A, Jarid1C, S. pombe Lid2 and Msc1 using the colour coding of Gibson et al, TiBS, 19, 349–53 1994. Conserved protein domains are indicated. (3.77 MB PDF)
Data
Purification of H2A.Z-TAP and H2A.Z-associated H2B for MS. (A) Coomassie stained SDS-PAGE gel and dot blot of fractions from the C4 RP-HPLC separation of H2A.Z-TAP in WT. The dot blot was probed with an antibody directed against the TAP-tag. Histones are indicated. (B) Chromatogram of RPHPLC separation (absorbance 214nm) in WT, msc1Δ, and swr1Δ. (5...
Article
Full-text available
Eukaryotic genomes are repetitively packaged into chromatin by nucleosomes, however they are regulated by the differences between nucleosomes, which establish various chromatin states. Local chromatin cues direct the inheritance and propagation of chromatin status via self-reinforcing epigenetic mechanisms. Replication-independent histone exchange...
Article
Full-text available
Linear motifs are short segments of multidomain proteins that provide regulatory functions independently of protein tertiary structure. Much of intracellular signalling passes through protein modifications at linear motifs. Many thousands of linear motif instances, most notably phosphorylation sites, have now been reported. Although clearly very ab...
Article
Full-text available
The Arabidopsis thaliana genome contains more than 30 genes encoding SET-domain proteins that are thought to be epigenetic regulators of gene expression and chromatin structure. SET-domain proteins can be divided into subgroups, and members of the Polycomb group (PcG) and trithorax group (trxG) have been shown to be important regulators of developm...
Article
A novel alignment-free method for computing functional similarity of membrane proteins based on features of hydropathy distribution is presented. The features of hydropathy distribution are used to represent protein families as hydropathy profiles. The profiles statistically summarize the hydropathy distribution of member proteins. The summation is...
Article
Full-text available
Epigenesis is the process whereby the daughters of a dividing cell retain a chromatin state determined before cell division. The best-studied cases involve the inheritance of heterochromatic chromosomal domains, and little is known about specific gene regulation by epigenetic mechanisms. Recent evidence shows that epigenesis pivots on methylation o...
Article
Full-text available
Ubiquitination serves as a key sorting signal in the lysosomal degradation of endocytosed receptors through the ability of ubiquitinated membrane proteins to be recognized and sorted by ubiquitin-binding proteins along the endocytic route. The ESCRT-II complex in yeast contains one such protein, Vps36, which harbors a ubiquitin-binding NZF domain a...
Chapter
IntroductionProtein ModulesFunctional Sites and Their Recognition ModulesRepresentation of Motifs and Functional SitesApplication of the Seefeld Convention to a Complex ExampleNew DirectionsReferences
Article
Structural similarity among proteins is reflected in the distribution of hydropathicity along the amino acids in the protein sequence. Similarities in the hydropathy distributions are obvious for homologous proteins within a protein family. They also were observed for proteins with related structures, even when sequence similarities were undetectab...
Article
The retinoblastoma tumor suppressor protein (pRb) regulates gene transcription by binding E2F transcription factors. pRb can recruit several repressor complexes to E2F bound promoters; however, native pRb repressor complexes have not been isolated. We have purified E2F/RBF repressor complexes from Drosophila embryo extracts and characterized their...
Article
Full-text available
The Saccharomyces cerevisiae WD-40 repeat protein Swd2p associates with two functionally distinct multiprotein complexes: the cleavage and polyadenylation factor (CPF) that is involved in pre-mRNA and snoRNA 3' end formation and the SET1 complex (SET1C) that methylates histone 3 lysine 4. Based on bioinformatic analysis we predict a seven-bladed be...
Article
The PHD finger and the bromodomain are small protein domains that occur in many proteins associated with phenomena related to chromatin. The bromodomain has been shown to bind acetylated lysine residues on histone tails. Lysine acetylation is one of several histone modifications that have been proposed to form the basis for a mechanism for recordin...
Article
Local differences in chromatin organisation may profoundly affect the activity of eukaryotic genomes. Regulation at the level of DNA packaging requires the targeting of structural proteins and histone-modifying enzymes to specific sites and their stable or dynamic interaction with the nucleosomal fiber. The "chromodomain", a domain shared by many r...
Article
Full-text available
Multidomain proteins predominate in eukaryotic proteomes. Individual functions assigned to different sequence segments combine to create a complex function for the whole protein. While on-line resources are available for revealing globular domains in sequences, there has hitherto been no comprehensive collection of small functional sites/motifs com...
Article
Full-text available
Histone 3 lysine 4 (H3 Lys4) methylation in Saccharomyces cerevisiae is mediated by the Set1 complex (Set1C) and is dependent upon ubiquitinylation of H2B by Rad6. Mutually exclusive methylation of H3 at Lys4 or Lys9 is central to chromatin regulation; however, S. cerevisiae lacks Lys9 methylation. Furthermore, a different H3 Lys4 methylase, Set 7/...
Article
Full-text available
The SET domain proteins, SUV39 and G9a have recently been shown to be histone methyltransferases specific for lysines 9 and 27 (G9a only) of histone 3 (H3). The SET domains of the Saccharomyces cerevisiae Set1 and Drosophila trithorax proteins are closely related to each other but distinct from SUV39 and G9a. We characterized the complex associated...
Article
The FYVE zinc finger domain is conserved from yeast (five proteins) to man (27 proteins). It functions in the membrane recruitment of cytosolic proteins by binding to phosphatidylinositol 3-phosphate (PI3P), which is found mainly on endosomes. Here we review recent work that sheds light on the targeting of FYVE finger proteins to PI3P-containing me...
Article
We propose a normalization of symbols and terms used to describe, accurately and succinctly, the detailed interactions between amino acid residues of pairs of interacting proteins at protein:protein (or protein:peptide) interfaces. Our aim is to unify several diverse descriptions currently in use in order to facilitate communication in the rapidly...
Article
Full-text available
Motivation: Gene expression is dependent on two main types of signals; one involving transcription factors which initiates gene transcription, and another which regulates the translation of a nascent mRNA. These post-transcriptional events play an important yet incompletely understood role in regulating gene expression and cellular behavior. Many...
Article
Full-text available
Set3 is one of two proteins in the yeast Saccharomyces cerevisiae that, like Drosophila Trithorax, contains both SET and PHD domains. We found that Set3 forms a single complex, Set3C, with Snt1, YIL112w, Sif2, Cpr1, and two putative histone deacetylases, Hos2 and NAD-dependent Hst1. Set3C includes NAD-dependent and independent deacetylase activitie...
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics & development.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.Contents (chosen by)525Cytoskeleton (Desai and Holleran)526Cell regulation (Roche, Servant and Weiner)528Nucleus and gene expression (Aasland and Weinzierl)529Membranes and sorting...
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.
Article
Full-text available
The binding of cytosolic proteins to specific intracellular membranes containing phosphorylated derivatives of phosphatidylinositol (PtdIns) is a common theme in vital cellular processes, such as cytoskeletal function, receptor signalling and membrane trafficking. Recently, several potential effectors of the phosphoinositide 3-kinase product PtdIns...
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in genetics and development.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in cell biology.
Article
Treatment of nuclei with limited amounts of DNaseI can be used to reveal sites in chromatin that are hypersensitive (HS) to the nuclease (1,2). DNaseI HS sites are thought to correspond to sites where the regular nucleosome structure is perturbed, e.g., by binding of proteins to chromatin such that DNA becomes more accessible to the nuclease. Some...
Article
The membrane lipid phosphatidylinositol-3-phosphate (PtdIns(3)P) is constitutively produced by yeast and higher eukaryotes through the phosphorylation of phosphatidylinositol by phosphatidylinositol-3-OH kinase (PI(3)K). PtdIns(3)P is important for vesicular transport, but little is known about how it acts, and proteins that specifically recognize...