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Regina Appiah-Opong

Regina Appiah-Opong
Noguchi Memorial Institute for Medical Research University of Ghana · Clinical Pathology

PhD

About

109
Publications
68,834
Reads
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1,282
Citations
Introduction
1. Drug discovery from natural sources (particularly anticancer and antimalarial agents), Cytochrome P450 mediated drug interactions studies 2. In vivo and in vitro assays including spectroscopic analysis 3. Currently working on anticancer and antimalarial agents and exploring their mechanisms of action.
Additional affiliations
April 2013 - present
Noguchi Memorial Institute for Medical Research, University of Ghana
Position
  • Head of Department
August 2011 - present
Noguchi Memorial Institute for Medical Research
Position
  • Ag. Head of Department
April 2004 - May 2009
Vrije Universiteit, Amsterdam
Position
  • PhD Student

Publications

Publications (109)
Article
Full-text available
Purpose Obesity and overweight are metabolic disorders associated with oxidative stress, and risk factors for many chronic diseases. We sought to investigate the effects of Metaswitch dietary supplement on weight gain and associated acute metabolic alterations in a high-fat diet-induced overweight rat model. Methods Female Sprague Dawley (SD) rats...
Article
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Croton membranaceus is a tropical plant that grows wildly in some West African countries. Though several parts of this plant are used for the treatment of diverse disease conditions, its root extracts are traditionally used mainly in Ghana for the management of prostate diseases. This present study aimed to investigate the in-vitro and in-vivo anti...
Article
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Introduction: Xylopic acid (XA), the major constituent of the fruit of Xylopia aethiopica, has shown several pharmacological properties. Traditionally, the plant is used to treat several diseases and is being used in the preparation of several local foods despite the lack of information about its safety, food-drug interaction, and other pharmacoki...
Article
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Cryptolepis sanguinolenta (Lindl.) Schlt., the main source of cryptolepine alkaloid, is intensively exploited in the wild to treat malaria and Lyme disease. In this study, the influence of four inorganic fertilizers (supplying N, P, K, or NPK) and four growth periods (3, 6, 9, and 12 months after transplanting) on the herb’s root biomass, cryptolep...
Article
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Malaria affects about half of the world's population. The sub-Saharan African region is the most affected. Plant natural products have been a major source of antimalarial drugs; the first (quinine) and present (artemisinin) antimalarials are of natural product origin. Some secondary metabolites demonstrate adjuvant antioxidant effects and selective...
Article
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Due to their rich bioactive secondary metabolites, herbal formulations sold in Nigeria are believed to have a curative effect on ailments including malaria. The study assessed the phytochemical properties, antioxidant properties, antiplasmodial, and cytotoxic effects of five commercially available herbal formulations. Preliminary phytochemical anal...
Article
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Objective: The main aim of this study was to investigate levels of total aflatoxin and aflatoxin M1 in bokina, a home-made non-alcoholic beverage prepared from dairy milk, millet and sugar. Methods: Bokina, dairy milk and millet were purchased monthly over a period of 7 months from bokina producers at Ashaiman and Nima, in Ghana. Total aflatoxin an...
Article
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Objective: The main aim of this study was to investigate levels of total aflatoxin and aflatoxin M1 in bokina, a home-made non-alcoholic beverage prepared from dairy milk, millet and sugar. Methods: Bokina, dairy milk and millet were purchased monthly over a period of 7 months from bokina producers at Ashaiman and Nima, in Ghana. Total aflatoxin an...
Article
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Background: Tieghemella heckelii stem bark is used in African traditional medicine to treat inflammatory pain conditions. However, these biological actions of the plant have not been proven. This study investigates the phytochemical composition and the mechanisms of analgesic and anti-inflammatory actions of the hydroethanolic stem bark extract of...
Article
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Most of the current cancer chemotherapeutics are associated with harsh and undesirable side effects, including toxicity and chemoresistance, driving the need for safer and more effective alternatives. In this study, the antiproliferative activities of the methanolic extract of Tetrapleura tetraptera fruits and nine different fractions (C1–C9) from...
Article
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Pericopsis laxiflora (Family Leguminosae) is a medicinal plant that is ubiquitous especially in the tropical and savannah regions. There are many reports on folkloric and traditional medicinal uses that include haemorrhoids, rheumatism, abdominal pain treatment, diarrhoea, and dysentery, fever, skin diseases and jaundice. Peer-reviewed articles wer...
Article
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Ethnopharmacological relevance Some local communities in Cote d'Ivoire use the mushroom Termitomyces schimperi combined with kaolin (TSK) to manage various cancers in patients. However, there is a paucity of data on toxicity, mutagenicity and trace metal constituent of TSK. Aim of the study We sought to investigate the acute and sub-chronic toxici...
Article
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The concentration of seven heavy metals in water samples was analyzed along the southwest coast of Ghana in the wet and dry seasons using atomic absorption spectrophotometer. Some physicochemical properties of the water samples were also determined to assess the quality of the water. The ranges of metal concentration in water in the wet season were...
Article
Anthonotha macrophylla leaf (AML) is employed in the management of pain, inflammation, and some diseases associated with oxidative stress in African traditional medicine. This study investigated the anti-inflammatory and analgesic activities, the acute toxicity and anti-oxidant properties of AML, characterised the phytochemical constituents, and qu...
Article
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Background: Nymphaea lotus L. (N. lotus) is an aquatic plant with anecdotal reports suggesting its use in the traditional management of cancer. However, there is a paucity of data on the antioxidant, anti-inflammatory and cytotoxic properties of N. lotus in relation to its phytochemical and elemental contents. This study aimed at determining the an...
Article
Background: Buruli ulcer (BU) is a neglected tropical disease caused by the Mycobacterium ulcerans. BU is an endemic disease in many communities in sub-Saharan Africa where population have long history of using medicinal plants for treatment. Indeed, several medicinal plants have been documented against BU and related conditions. The present study...
Article
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Background Ghana is endemic for some neglected tropical diseases (NTDs) including schistosomiasis, onchocerciasis and lymphatic filariasis. The major intervention for these diseases is mass drug administration of a few repeatedly recycled drugs which is a cause for major concern due to reduced efficacy of the drugs and the emergence of drug resista...
Presentation
Chemotherapy-induced oxidative stress (CIOS) plays a critical role in de novo cancer initiation and development. We hypothesised that preventing CIOS could reduce or prevent the advent of cancers induced by chemotherapeutic agents such as methotrexate (MET) and docetaxel (DOC). Riboceine (RIB) has been shown to replenish the decreased level of glut...
Article
Ethnopharmacological relevance: Malaria is caused by infection with some species of Plasmodium parasite which leads to adverse alterations in physical and hematological features of infected persons and ultimately results in death. Antrocaryon micraster is used to treat malaria in Ghanaian traditional medicine. However, there is no scientific valid...
Article
Ethnopharmacological relevance Malaria is a global public health burden due to large number of annual infections and casualties caused by its hematological complications. The bark of Annickia polycarpa is an effective anti-malaria agent in African traditional medicine. However, there is no standardization parameters for A. polycarpa. The anti-malar...
Article
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Objective: Synedrella nodiflora is traditionally used in the treatment of several ailments. Pharmacologically, this plant has anticonvulsant, sedative, anti-nociceptive and anti-proliferative effects. This study further investigated S. nodiflora for its antioxidant and in vitro inhibition of cancerous cell lines. Methods: Phytochemical assays, and...
Article
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Background and aim Most developing countries resort to medicinal plants for treating diseases, but few of these have scientific backing for their use. The aim of the study was to validate traditional use of Morinda lucida leaves in treating inflammation and determine the mechanism of action. Experimental procedure Effect of hydroethanolic leaf ext...
Article
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COVID-19 is a disease that is caused by a pathogen known as coronavirus 2 (SARS-CoV-2). Coronaviruses belong to a superfamily of viruses which infect both humans and animals. These viruses are positive-stranded RNA viruses that are enveloped and replicate in the cytoplasm. Their envelope fuses with the host cell membrane and then the nucleocapsid i...
Article
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Background: Globally, interest in herbal medicines is increasing. In Ghana, most herbalist use herbal medicines for treatment of various ailments including prostate cancer, although no empirical evidence on their efficacies exists. Objective: The aim of the presentstudy was to test for antioxidant and anti-prostate cancer activities of Moringa olei...
Article
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Background: There is considerable evidence that many patients concurrently administer dietary supplements with conventional drugs, creating a risk for potential drug-supplement interaction. The aim of this study was to determine the effect of Cellgevity® supplement on selected rat liver cytochrome P450 (CYP) enzymes. Also, based on our previous fi...
Article
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Introduction : Malaria remains a life-threatening disease, mainly in tropical and sub-tropical countries of the world. The problem caused by the disease is further compounded by the emergence and spread of multidrug resistant Plasmodium falciparum . Coupled with the poor distribution of modern health facilities, there is resurgence in the use of he...
Article
Aim: To investigate the antileishmanial potency of Sargassum vulgare C. Agardh-derived products and the in silico inhibition of trypanothione reductase by fucosterol. Materials & methods: Sargassum vulgare crude extract and its derived fractions, subfractions and fucosterol were screened against Leishmania major and Leishmania donovani using the MT...
Article
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Many developing countries depend on herbal mixtures as the first line and cost-effective therapy for malaria. These mixtures with such curative tendencies may also be a source of toxicity to host cells. On the other hand, these mixtures may have anticancer potential activity characterized by cytotoxicity to cancer cells. The aim of the study was to...
Article
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Phytochemical investigation of the ethanol extract of the stem bark of Hypericum lanceolatum Lam. (Guttifereae) afforded eight known compounds including 2,2ʹ,5,6ʹ-tetrahydroxybenzophenone (1), 5-hydroxy-3-methoxyxanthone (2), 3-hydroxy-5-methoxyxanthone (3), betulinic acid (4), hydroquinone (5) 6,7-dihydroxy-1,3-dimethoxyxanthone (6), calophyllumin...
Preprint
Full-text available
Background There is considerable evidence that many patients concurrently take dietary supplements with conventional drugs, with a risk of potential drug-supplement interaction. The aim of this study was to determine the effect of Cellgevity® supplement on selected rat liver cytochrome P450 (CYP) enzymes and on the pharmacokinetics of carbamazepine...
Preprint
Full-text available
Background: There is considerable evidence that many patients concurrently administer dietary supplements with conventional drugs, creating a risk for potential drug-supplement interaction. The aim of this study was to determine the effect of Cellgevity® supplement on selected rat liver cytochrome P450 (CYP) enzymes. Also, we sought to deternine th...
Article
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Background: Malaria is one of the most important life-threatening infectious diseases in the tropics. In spite of the effectiveness of artemisinin-based combination therapy, reports on reduced sensitivity of the parasite to artemisinin in Cambodia and Thailand warrants screening for new potential antimalarial drugs for future use. Ghanaian herbali...
Article
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Schistosomiasis is a neglected tropical disease that affects 200 million people and accounts for 100,000 deaths annually. In endemic geographical areas, schistosomiasis has been implicated as an etiological agent in the pathogenesis of bladder, colorectal, and renal carcinoma largely due to Schistosoma eggs in tissues that comes with chronic infect...
Article
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Drug interactions are key reasons for adverse drug reactions and attrition from market. Major infectious diseases causing morbidity/mortality in Ghana are malaria, tuberculosis, and HIV/AIDS. In this study, plant medicines commonly used to treat/manage these diseases in Ghana were investigated for their potential to modulate rat cytochrome P450 enz...
Article
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There are numerous herbal products on the Ghanaian market that are purported to cure various ailments, including cancer. However, scientific investigations on efficacy and toxicity of most of these products are not done. The aim of the study was to assess the anticancer potentials of herbal products on the Ghanaian market. Antiproliferative effects...
Article
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Background There is considerable evidence that many people take dietary supplements including those of herbal origin as an alternative therapy to improve their health. One such supplement, with an amalgam of constituents, is CellGevity®. However, the effect of this dietary supplement on drug-metabolizing enzymes is poorly understood, as it has not...
Article
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Trypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands of new cases recorded annually. Control of these infection(s) with existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence of resistant strains. Medicinal plants on the other ha...
Article
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Leishmaniasis is a vector-borne neglected tropical disease caused by protozoan parasites of the Leishmania genus and transmitted by the female Phlebotomus and Lutzomyia sand flies. The currently prescribed therapies still rely on pentavalent antimonials, pentamidine, paromomycin, liposomal amphotericin B, and miltefosine. However, their low efficac...
Article
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New therapies for leukemia are urgently needed due to adverse side effects, tumor resistance and lack of selectivity of many chemotherapeutic agents in clinical use. Ageratum conyzoides has been used in folklore medicine for managing leukemia and other cancers. Thus, this study aimed to investigate the effects of fractions, sub-fractions and purifi...
Article
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As part of our search for bioactive compounds from the Dichapetalaceae, repeated chromatographic purification of the roots of a hitherto unexamined species, Dichapetalum pallidum, led to the isolation of the newly occurring 7-hydroxydichapetalin P (1) and the known dichapetalins A (2) and X (3). Also isolated were the known compounds friedelin-2,3-...
Article
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Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schi...
Article
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There is an urgent need for new anti-malaria drugs with broad therapeutic potential and novel mode of action, for effective treatment and to overcome emerging drug resistance. Plant-derived anti-malarials remain a significant source of bioactive molecules in this regard. The multicomponent formulation forms the basis of phytotherapy. Mechanistic re...
Article
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The high cost of treatment of cancer coupled with the emergence of drug resistance makes it imperative for new drug interventions to curb its occurrence. Hence, the objective of this research was to determine the phytochemical, total phenolic content, antioxidant and antiproliferative effect of Codiaeum variegatum crude extracts and fractions. The...
Article
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Aim and objectives: Buruli ulcer (BU) is a neglected tropical disease caused by a mycobacteria, Mycobacterium ulcerans. The WHO recommended Rifampicin-Streptomycin combination side effects and poor compliance, leaves rural populations with no choice than to patronise indigenous remedies. This study is aimed at validating medicinal plants used in t...
Article
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Objective/background: Mycobacterial infections including tuberculosis, leprosy, and buruli ulcer are among the most prevalent, debilitating, and deadly tropical diseases, especially in Sub-Saharan Africa. The development of drug resistance to the currently available drugs and the poor compliance emphasize the need for new chemotherapeutic agents....
Article
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Leishmaniasis is an infectious disease transmitted by the sand fly. It is caused by over 20 different species of Leishmania and has affected over 14 million people worldwide. One of the main forms of control of leishmaniasis is chemotherapy, but this is limited by the high cost and/or toxicity of available drugs. We previously found three novel com...
Article
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Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a serious public health challenge towards which new hits are urgently needed. Medicinal plants remains a major source of new ligands against global infectious illnesses. In our laboratories, we are currently investigating locally used ethnobotanicals for novel compounds against zoonotic...
Article
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Buruli ulcer (BU) is the third most prevalent mycobacteriosis, after tuberculosis and leprosy. The currently recommended combination of rifampicin-streptomycin suffers from side effects and poor compliance, which leads to reliance on local herbal remedies. The objective of this study was to investigate the antimycobacterial properties and toxicity...
Article
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This article contains data on in vitro antimycobacterial activity and cytotoxicity of hydroethanolic crude extracts from five selected medicinal plant species traditionally used to treat tuberculosis in Ghanaian ethnomedicine, see “Medicinal plants used to treat TB in Ghana” [1]. The interpretation and discussion of these data and further extensive...
Article
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Trypanosoma brucei parasites are a group of kinetoplastid protozoa which devastate health and economic well-being of millions of people in Africa through the disease, Human African Trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects and drug resistance issues plaguing current drugs. Recently, there have been...
Article
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For centuries now, plants have served as a great source of compounds with pharmacological properties. Some plants like weeds, however, have not been fully exploited for their medicinal value. In this research, the phytochemical, total phenolic content, antioxidant and antiproliferative effect of Crotalaria retusa, a weed reported to have potential...