Reggie Gaudino

Front Range Biosciences · R&D

We are working toward a complete functional and genomic characterization of the cannabinoid synthase family in Cannabis (itself part of the larger Berberine Bridge Enzyme family). This clade, which is unique to Cannabis, includes four main subclades that appear to have arisen by a series of gene duplications. We have functionally characterized thre...

Background Cannabis sativa is a primarily dioecious angiosperm that exhibits sexual developmental plasticity. Developmental genes for staminate male flowers have yet to be elucidated; however, there are regions of male-associated DNA from Cannabis (MADC) that correlate with the formation of pollen producing staminate flowers. MADC2 is an example of...

This technology relates in part to methods of identifying plant cultivars based on the terpene synthase genes that are identified and/ or quantified (e.g., copy number, ploidy) in the cultivars. The methods provided herein permit plant cultivars with desired characteristics/ phenotypes, e.g., a desired terpene production profile, to be selected for...

Terpene oil profiling and Clade classification for Cannabis based on shared terpene profiles. The Methodology allows for identification/selection of cannabis for specific applications (Agricultural, Medicinal) based on the terpene profile

The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in cannabis plants. In some asp...

The widespread legalization of Cannabis has opened the industry to using contemporary analytical techniques for chemotype analysis. Chemotypic data has been collected on a large variety of oil profiles inherent to the cultivars that are commercially available. The unknown gene regulation and pharmacokinetics of dozens of cannabinoids offer opportun...

The accelerating legalization of Cannabis has opened the industry to using contemporary analytical techniques. The gene regulation and pharmacokinetics of dozens of cannabinoids remain poorly understood. Because retailers in many medical and recreational jurisdictions are required to report chemical concentrations of cannabinoids, commercial labora...

Gene copy number (CN) variation is known to be important in nearly every species where it has been examined. Alterations in gene CN may provide a fast way of acquiring diversity, allowing rapid adaptation under strong selective pressures, and may also be a key component of standing genetic variation within species. Cannabis sativa plants produce a...

Terpenes are responsible for most or all of the odor and flavor properties of Cannabis sativa, and may also impact effects users experience either directly or indirectly. We report the diversity of terpene profiles across samples bound for the Washington dispensary market. The remarkable degree of variation in terpene profiles ultimately results fr...

Gene copy number variation is known to be important in nearly every species where it has been examined. Alterations in gene copy number may provide a fast way of acquiring diversity, allowing rapid adaptation under strong selective pressures, and may also be a key component of standing genetic variation within species. Cannabis sativa plants produc...

This poster describes our team's work into analyzing whole genome shotgun data towards various breeding applications.

Describes Steep Hill Genetics Team's work into cloning the cannabinoid synthase family and functionally identifying the product profiles of each variant we studied.

Compositions and methods for providing desired cannabinoid content in Cannabis plants. More particularly, the invention relates to compositions and methods for using cannabinoid synthase paralogs as guidance for breeding Cannabis plants with a desired cannabinoid content, including but not limited to cultivars, varieties, lines, and methods of bree...

As the most widely used illicit drug worldwide, and as a source of numerous under-studied pharmacologically-active compounds, a precise understanding of variability in psychological and physiological effects of Cannabis varieties is essential. The National Institute on Drug Abuse (NIDA) is designated as the sole legal producer of Cannabis for use i...

As the most widely used illicit drug, the basis of the fastest growing major industry in the US, and as a source of numerous under-studied psychoactive compounds, understanding the psychological and physiological effects of Cannabis is essential. The National Institute on Drug Abuse (NIDA) is designated as the sole legal producer of Cannabis for us...

RNA polymerase I (pol I) transcribes the repeated genes that encode the precursor of 17-18, 5.8, and 25-28 S ribosomal RNA (rRNA). Pol I transcription is up-regulated in growing cells and down-regulated in quiescent cells, presumably reflecting the demand for ribosomes and protein synthesis. However, the signal transduction pathways responsible for...

In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement of functional elements, such a...

Termination of transcription by the RNA polymerase of E. coli is generally assumed to be quite limited in the absence of deliberate transcription termination signals. However, the inherent termination rate of RNA polymerase in the absence of deliberate termination signals (what we will refer to as the processivity of RNA polymerase) has not been ca...

Thesis (Ph. D.)--State University of New York at Buffalo, 1993. Includes bibliographical references (leaves 233-250). Photocopy of typescript.