Reda Salem

• Doctor of Philosophy
• Researcher at Agricultural Genetic Engineering Research Institute

Psorosis is a damaging disease of citrus that is widespread in many parts of the world. Citrus psorosis virus (CPsV), the type species of the genus Ophiovirus, is the putative causal agent of psorosis. Detection of CPsV by laboratory methods, serology in particular is a primary requirement for large-scale surveys but their production has been impai...

Serological assays for SARS-CoV-2 are being utilized at an exponential rate for surveillance programs. This enterprise was designed to develop and validate a qualitative immunochromatographic test, via the Lateral Flow Assay (LFA), for detection of immunoglobulins M and G (IgM and IgG) against both nucleocapsid (N) and the receptor-binding domain (...

One of the most important factor that affects the efficient using of baculoviruses as a biopesticide is their sensitivity to UV irradiation. In this study, a photolyase gene ( phr ) of 1.4 kbp DNA fragment was cloned and characterized from Spodoptera littoralis granulovirus, an Egyptian isolate (SpliGV-EG1). A sequence of 466 amino acid were deduce...

As the second wave of COVID-19 launched, various variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) have emerged with a dramatic global spread amongst millions of people causing unprecedented case fatalities and economic shut-downs. That initiated a necessity for developing specific diagnostics and therapeutics along with vacc...

During an ongoing outbreak of Foot-and-Mouth Disease Virus (FMDV), it is crucial to distinguish naturally infected from vaccinated seropositive animals. This would support clinical assessment and punctual vigilance. Assays based on 3ABC non-structural protein as an antigen are reliable for this intention. However, the insolubility and degradation o...

Abstract Two different Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) nucleopolyhedrovirus (SpliNPV) isolates were obtained from natural infected S. littoralis larvae in Egypt. The phylogenetic analysis of the partial nucleotide sequence of the polyhedrin gene (polh) showed that both isolates, Spli-6 and Spli-7, were closed and had a commo...

The search for effective and bioactive antimicrobial molecules to encounter the medical need for new antibiotics is an encouraging area of research. Plant defensins are small cationic, cysteine-rich peptides with a stabilized tertiary structure by disulfide-bridges and characterized by a wide range of biological functions. The heterologous expressi...

Foot-and-mouth disease virus (FMDV) is one of the most devastating animal viruses that affect livestock worldwide. The 1B capsid of FMDV has been widely used to detect and confirm the infection. In the present study, the sequence coding for 1B subunit of FMDV capsid was expressed in insect cells using the baculovirus expression system under the pol...

This study aimed to test and evaluate antibacterial and antifungal activities of secondary metabolites obtained from endophytic fungi isolated from the leaves of endemic plant Zygophyllum mandavillei. The fungus Cladosporium cladosporioides was the predominant isolated fungus with colonization and dominance frequencies percentage of 12.50 and 39.32...

Keywords: Infectious bursal disease virus (IBDV) Chimeric protein IgY fragment crystallizable (Fc) VP2 projection domain (PD) Transgenic maize Immature embryos Biolistic bombardment A B S T R A C T Control of Infectious bursal disease virus (IBDV) in endemic countries has been based on early immunization of chicks using conventional live or inactiv...

Throughout the vegetative life of Bacillus thuringiensis, vegetative insecticidal proteins (Vip) are produced and secreted. In the present study, the vip3 gene isolated from Bacillus thuringiensis, an Egyptian isolate, was successfully amplified (2.4 kbp) and expressed using bacterial expression system. The molecular mass of the expressed protein w...

Foot-and-mouth disease virus (FMDV) is one of the most devastating viral pathogens of cloven-hoofed animals. The detection of antibodies (Ab) against FMDV structural proteins (SP) using virus neutralization test (VNT) and liquid-phase blocking ELISA (LPBE) is the standard procedure in use for monitoring seroconversion in animals post vaccination, t...

Foot-and-mouth disease virus (FMDV) exhibits a high degree of antigenic diversity among its serotypes, requiring several anti-FMDV antibodies for its laboratory diagnosis, which complicated the used techniques. To conquer this cumbersome, we developed a new panel of different single-chain fragment variable (scFv) for serotype-independent detection...

DNA encoding the coat protein (CP) of an Egyptian isolate of tomato yellow leaf curl virus (TYLCV) was inserted into the genome of Autographa californica nucleopolyhedrovirus (AcNPV) under the control of polyhedrin promoter. The generated recombinant baculovirus construct harboring the coat protein gene was characterized using PCR analysis. The rec...

Hairy root (HR) cultures derived from Agrobacterium rhizogenes (A. rhizogenes) transformation of plant tissues are an advantageous biotechnological manufacturing platform due to the accumulation of recombinant proteins in an otherwise largely protein free culture medium. In this context, HRs descending from transgenic Nicotiana tabacum (N. tabacum)...

Background Wheat is considered the most important cereal crop all over the world. The wheat weevil Sitophilus granarius is a serious insect pests in much of the wheat growing area worldwide and is responsible for significant loss of yield. Avidin proteins has been proposed to function as plant defense agents against insect pests. Results A synthet...

Sensitive diagnostic tools are ur-gently needed for rapid and specific virus detection. Pepper Leaf Curl Virus (PeLCV) particles were purified from the injected pepper plants after symptoms manifestation. PeLCV particles were characterized by injection into healthy pepper plants which exhibited typical in-fection symptoms including curling and down...

Apollon, also called Baculoviral IAP Repeat-Containing Protein 6 (BIRC6) or Baculoviral IAP Repeat-containing Ubiquitin Conjugating Enzyme (BRUCE), is an anti-apoptotic protein belonging to the IAP family, which consists of eight members. The genes of this family render cancer cells insensitive to apoptotic stimulation. The aim of the present study...

This study was designed to use the recombinant expressed CMV/coat protein (CMV/CP) as an antigen for producing specific polyclonal antibodies (PAbs) in high concentration and free from plant contaminants. The coat protein gene was subcloned into a bacterial expression vector (pQE- 32) and expressed as a fusion protein with an N-terminal hexa-histid...

Cucumber mosaic virus was de-tected in banana plants showing typical symptoms of CMV infection using DAS-ELISA. Coat protein cDNA was ampli-fied, by RT-PCR using specific primers, and cloned into pGEM®-T Easy Vector, then transformed into E. coli Top 10 cells. The complete sequence of coat protein gene from CMV-Egy strain has been de-termined and c...

Citrus psorosis ophiovirus (CPsV) was detected from naturally citrus cv. Navel orange trees exhibited virus-like symptoms (bark scaling and gum) at Qanater, Qaluobia governorate, Egypt. CPsV isolate was detected by serological assay using monoclonal antibodies (Mabs) specific to CPsV by double antibody sandwich- enzyme linked immunosorbent assay (D...

Citrus tristeza virus (CTV) is one of the most destructive and economically important diseases of commercial citrus worldwide. In this study, the coat protein (cp) gene of CTV was subcloned into PQE-30 vector at Bam HI and Hind-III restriction sites. The constructed plasmid that called pRMAF2 was transformed into Escherichia coli M15 strain for its...