Ravishankar Chauhan

• Doctor of Philosophy
• Professor (Assistant) at Pandit Ravishankar Tripathi Government College Bhaiyathan, Surajpur 497231, INDIA

A simple, in vitro multiplication and mid-term conservation system was developed for Chlorophytum borivilianum Sant. et Fernand, which is documented as a critically endangered herb on the International Union for Conservation of Nature and Natural Resources red list. In vitro shoot multiplication was achieved by culture on Murashige and Skoog medium...

Genus Chlorophytum Ker Gawl secures its position chiefly as commercial plants with a wide range of applications, right from pharmaceutical to ornamental, and with a promising economical return also. Few species of this genus are now enlisted under “threatened plant category” due to rash harvesting from its wild habitat, as it is utilized extensivel...

Diosgenin, one of the steroidal sapogenin, is a chief bioactive compound, commercially known for its pharmaceutical application to treat sexual dysfunction. The biosynthesis of plant secondary metabolites in vitro cultures is usually manipulated by different elicitors that lead to higher production than non-elicited cultures. In the present investi...

In vitro approaches are valuable for the conservation of plant biodiversity that includes the preservation of genetic resources of vegetatively propagated species, threatened plant species, taxa with recalcitrant seed, elite genotypes, and genetically modified/engineered material. The mid-term conservation is usually achieved by reducing the growth...

Here, we report an ultrasonic-assisted extraction (UAE) of phytochemicals from bark, leaves, sepals, fruits, and seeds of Dillenia pentagyna (Roxb) using different organic solvents such as chloroform, ethanol, and n-hexane. The preliminary phytochemical screening results showed that the ethanolic extract is enriched with phenolics, flavonoids, tann...

Medicinal applications of Plumbago zeylanica and its metabolites on various diseases and low viability and inconsistent germination of its seeds are the reasons behind the loss of its genetic diversity. Hence, an efficient protocol for the short-term storage of P. zeylanica synthetic seeds, which is an overexploited medicinally valuable plant, was...

Determination of heavy metals in soil, water, food, pharmaceuticals and environment samples has received great attention due to their serious threat to the ecology and human health. Indeed, technological advancements led to the rapid and real time detection and quantification of heavy metals in various samples. In this chapter, we focused on instru...

Here, we summarize phytochemicals, their health benefits, pharmacokinetics and pharmacodynamic characteristics, toxicity, and their application in the development of lead compounds and related drug analogs. Natural products and their structural analogues have significant contributions to pharmacotherapy, especially for the treatment of cancer, mala...

A fast-growing, leguminous tree species- Pithecellobium dulce (Roxb.) Benth., was screened in vitro against Pb-stress for the first time. In the current study, Pb toxicity affected the seedlings growth, lipid peroxidation, hydrogen peroxide production, antioxidant enzymes activity, proline content, and genomic template stability in a dose-dependent...

Pleurotus ostreatus is generally known as oyster mushroom that comprised in the category of edible mushroom. Various grain spawns were chiefly utilized for its commercial production. However, these spawns have several limitations such as high production cost and more contamination rate. Henceforth, the current study dealt with the development of st...

In developing countries, rapid urbanization and industrialization cause heavy metal contamination, including lead (Pb). India is one of the most developing countries where anthropogenic sources are the chief generators of Pb contaminants. Mining, smelting Pb containing paints, papers, gasoline, and municipal sewage sludge enriched with Pb come in c...

Ammonium nitrate (AN) is one of the major nitrogen sources of Murashige and Skoog (MS) medium. It is prohibited in various countries, including India because it is used in explosive manufacturing. Since MS is the most successful medium used for in vitro culture of many plant species, an attempt was made to achieve the composition of MS medium using...

Phoma is an exceptionally polyphyletic genus of rapidly growing soil fungi under the phylum of Ascomycota. The genus of Phoma includes more than 2000 species observed as phytopathogen or endophytes. The endophytic Phoma spp. have been isolated from a range of tropical/subtropical plants, arid climate of mangrove, mountain, desert, and forest. Wide...

This paper describes the sensing application of citrate functionalized gold nanoparticles (AuNPs) employing for the determination of L-cysteine in food and water samples. It is established with diffuse reflectance Fourier transform infrared (DRS-FTIR) spectroscopic analysis. The disappearance of the thiol (-SH) band in the FTIR spectra and the shif...

Golden yellow flowering Butea monosperma was recorded for the first time in the district Rajnandgaon near Ambagarh Chowki. The plant is identified as Butea monosperma (Lam) Taub. Syn B. Frondosa Koenig Ex. Roxb.var. lutea (Witt) Maheshwari. The location of the plant is 20° 49' 19.1"N; 80° 44' 45.8"E. On-site observation and morphological characteri...

This article reports the cryopreservation of apical meristems of Chlorophytum borivilianum, a tropical and IUCN critically endangered species. Initially, in vitro cultured shoots were pre-adapted on 12% (w/v) sucrose for 2-months and were found appropriate stock material for further experimentations. Furthermore, the preculture of meristems excised...

Four (4) prominently growing tropical tree species were screened for their tolerance to heavy metals and evaluated for their suitability for remediation, at a contaminated site. The partitioning of Iron (Fe) and Lead (Pb) between roots and above-ground aerial parts of the trees, bioconcentration factor (BCF) and translocation factor (TF) were used...

In vitro germination of hybrid seeds of papaya (Carica papaya Linn.) was attempted in the present investigation. Hybrid papaya seeds were subjected to three kinds of treatments. First on semisolid medium Murashige and Skoog (1962), secondly on only inorganic salt solution of the same medium but without organic nutrients and with various combination...