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Introduction
Publications
Publications (15)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi’s sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle of KSHV. KSHV infection promotes HIF-1α activity, and several KSHV g...
Protein levels of metabolic genes unchanged by HIF-1α knockdown.
Protein levels of HK-2, Lipin 1, and FasN were measured in shScr and shHIF-1 cells after 48 hours in normoxia (N) or hypoxia (H). β-actin is shown as a loading control.
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Effect of hypoxia on the levels of KSHV miRNAs in BCBL-1 cells.
Levels of mature KSHV miRNAs were measured using taqman assays. The miRNA levels were normalized to that of RNU43 internal miRNA control and the results are expressed as fold change over normoxia.
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Effects of HIF-1α inhibitor PX-478 on VERO and VERO-K cells.
VERO and VERO-K cells were plated at 5x104 cells per well of a 6-well plate. Indicated amounts of PX-478 were added after 24 hours, and proliferation rates and numbers of live cells were measured 72 hours post-treatment. (A) Proliferation rates of VERO or VERO-K cells measured using the M...
List of antibodies used in Western blot analysis.
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PEL cells are sensitive to inhibition of glycolysis.
Cells were treated with various concentrations of glycolysis inhibitor 2-DG for 24 hours and rate of proliferation (A) and cell death (B) were measured using MTS assay and trypan blue exclusion method, respectively. Results are expressed as fold changes compared to no 2-DG control. Error bars rep...
Levels of cMyc is unchanged by HIF-1α knockdown.
(A) mRNA levels of cMyc in BCBL-1 cells after 48 hours in normoxia (N) or hypoxia (H), normalized to 18S internal control and expressed as fold changes compared to shScr cells in N. (B) Protein levels of cMyc in the nuclear lysates of BCBL-1 and BC-3 cells after 48 hours in N or H. β-actin is shown a...
List of PrimePCR SYBR Green q-PCR Assays from Bio-Rad.
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List of TaqMan MicroRNA Assays used for RT-qPCR of mature miRNAs.
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List of primers used for RT-qPCR of mRNAs.
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LANA protein levels.
Western blot of LANA in the nuclear extracts of BCBL-1 cells cultured for 48 hours. β-actin is shown as a loading control.
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Importance:
Kaposi sarcoma herpesvirus (KSHV)-associated multicentric Castleman disease (KSHV-MCD) is characterized by severe inflammatory symptoms caused by an excess of cytokines, particularly KSHV-encoded viral interleukin-6 (vIL-6) produced by lymph node plasmablasts. vIL-6 is usually a lytic gene. We show that a number of KSHV-MCD lymph node...
Hypoxia activates KSHV lytic replication in primary effusion lymphoma (PEL) cells. In the current study, we show that LANA mRNA levels were up regulated in BC-3 PEL cells in hypoxia. Further, the total levels of LANA protein were elevated in BC-3 PEL cells after 24 h hypoxia and increased through 72 h. Also, infection of BC3 cells with a retroviral...
Hypoxia and hypoxia-inducible factors (HIFs) play an important role in the Kaposi's sarcoma-associated herpesvirus (KSHV) life cycle. In particular, hypoxia can activate lytic replication of KSHV and specific lytic genes, including the replication and transcription activator (RTA), while KSHV infection in turn can increase the levels and activity o...
Hypoxia inducible factors (HIFs) are the principal means by which cells upregulate genes in response to hypoxia and certain other stresses. There are two major HIFs, HIF-1 and HIF-2. We previously found that certain genes are preferentially activated by HIF-2. One was protein tyrosine phosphatase, receptor-type, Z polypeptide 1 (PTPRZ1). PTPRZ1 is...
