Ravi Rajwanshi

Ravi Rajwanshi
Indira Gandhi National Open University (IGNOU) | IGNOU · Discipline of Life Sciences

M.Sc., Ph.D.
Associate Professor, Discipline of Life Sciences, School of Sciences, IGNOU, New Delhi, India

About

64
Publications
12,668
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,109
Citations
Introduction
Dr. Ravi Rajwanshi currently works at the Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University (IGNOU), New Delhi, India. Ravi does research in Plant Molecular Biology, Biochemistry and Biotechnology, Life Sciences.
Additional affiliations
August 2021 - present
Indira Gandhi National Open University (IGNOU)
Position
  • Professor (Associate)
Description
  • Presently I am working as Associate Professor in the Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University (IGNOU), Maidan Garhi, New Delhi-110068, India
March 2010 - August 2021
Assam University (A Central University)
Position
  • Professor (Assistant)
Description
  • Biotechnology, Plant Molecular Biology, Biochemistry, Agricultural Biotechnology, Life Sciences.
March 2009 - March 2010
Indian Council of Agricultural Research
Position
  • Research Associate
Description
  • DBT Project
Education
August 2002 - December 2008
August 2000 - July 2001
Jamia Millia Islamia
Field of study
July 1997 - August 1999
Jamia Millia Islamia
Field of study

Publications

Publications (64)
Article
Full-text available
Key message Small RNAs that are highly conserved across many plant species are involved in stress responses. Abstract Plants are exposed to many types of unfavorable conditions during their life cycle that result in some degree of stress. Recent studies on microRNAs (miRNAs) have highlighted their great potential as regulators of stress toleranc...
Thesis
The amount of land available for crop production is decreasing steadily due to urban growth and land degradation. This decrease in the amount of arable land available for crop production and increase in human population will have major implications for food security over the next 2-3 decades. A vast tract of agricultural land in India is presently...
Article
Full-text available
Tocopherols (vitamin E) are lipid soluble antioxidants synthesized by plants and some cyanobacteria. We have earlier reported that overexpression of the γ-tocopherol methyl transferase (γ-TMT) gene from Arabidopsis thaliana in transgenic Brassica juncea plants resulted in an over six-fold increase in the level of α-tocopherol, the most active form...
Article
Full-text available
Background The MYB gene family comprises one of the richest groups of transcription factors in plants. Plant MYB proteins are characterized by a highly conserved MYB DNA-binding domain. MYB proteins are classified into four major groups namely, 1R-MYB, 2R-MYB, 3R-MYB and 4R-MYB based on the number and position of MYB repeats. MYB transcription fact...
Article
Full-text available
The use of antibiotic and herbicide resistance based negative selection in plant transformation experiments remains a major impediment in the acceptance of transgenic crops. To overcome this, Positech selection system involving the use of phosphomannose isomerase (pmi) gene from Escherichia coli and mannose as selection agent was exploited for the...
Article
Plants are subjected to various environmental stresses that influence their growth, metabolism, productivity and survival. Nevertheless, plants respond to these stresses by inducing certain morpho-physiological, biochemical, molecular and genetic changes. Some of these changes conferring stress tolerance include the formation of cork/abscission lay...
Article
We previously generated Brassica juncea lines overexpressing either glyoxalase I (gly I) or γ-tocopherol methyltransferase (γ-TMT) involved in the glyoxalase system and tocopherol biosynthesis, respectively. These transgenic plants showed tolerance to multiple abiotic stresses. As tolerance is a complex trait that can be improved by pyramiding of s...
Chapter
In the present era, human population-driven activities are posing a severe threat to biota leading to loss of biodiversity at an unprecedented rate. If the loss of biodiversity continues at the current pace, then the present world would enter mass extinction. Various ex-situ and in-situ conservation strategies have been undertaken but have not succ...
Chapter
In recent years, the development of transgenic Brassica juncea with enhanced tolerance to biotic and abiotic stress conditions has been reported by several research groups worldwide. Herein, we present an exhaustive compilation of the studies published on this subject, with a special focus on long noncoding RNAs and development of regulatory genes...
Chapter
Natural flavoring industry has undergone an immense surge in production that has done more than enough to compete and even supersede the chemically synthesized flavoring industry, due to a rapid increase in demand from the consumer. The natural flavoring industry utilizes techniques like plant tissue culture, de novo synthesis, and biotransformatio...
Article
Climate change negatively impacts the global hydrological resources leading to detrimental flood events. Submergence impedes the cellular membrane integrity, consequently affecting the membrane fluidity. Different abiotic stresses influence membrane lipid composition. Therefore, the remodeling of membrane lipids plays a major role in stress adaptat...
Article
Full-text available
Twenty three collections of Mucuna pruriens were evaluated for their genetic variability in ten morphological traits. Analysis of variance for all the 10 characters indicated statistically significant variation among the collections. Collection WBNMP-06 produced highest seed yield (2.47 t/ha) followed by UPMP-06 (2.22 t/ha) and WBNMP-03 (2.00 t/ha)...
Article
Nitric oxide (NO) plays a significant role in plants under different abiotic stress. However, the influence of NO and other reactive nitrogen species (RNS) in employing nitrosative stress in rice seedlings under submergence remains unexplored. Submergence also stimulates the development of lysigenous aerenchyma, facilitating oxygen supply to roots...
Chapter
Full-text available
Brassicaceae family includes a large number of commercially important plants, which are oilseed crops, vegetables, forage crops and are used in the production of condiments. Among the Brassica crops, oilseeds have the highest economic value and thus have garnered much importance for their improvement by biotechnological methods. This chapter explor...
Chapter
Full-text available
MicroRNAs are a class of small RNAs that play a pivotal role in post-transcriptional gene regulation. The role of miRNA in fine-tuning gene expression is essential for growth and development of the plant as well as to cope up with various abiotic and biotic stress conditions. Plant immunity is a well-regulated and complex system. Plants initiate a...
Article
Full-text available
MicroRNAs (miRNAs) are a class of small non-coding RNA of approximately 22 nucleotides in length derived from one arm of hairpin stem-loop secondary precursors, that regulates gene expression at the transcriptional or post-transcriptional level either through cleavage or translation inhibition of the target mRNA. In the present study, an attempt wa...
Article
Full-text available
Rice being a staple cereal is extremely susceptible towards abiotic stresses. Drought and salinity are two vital factors limiting rice cultivation in Eastern Indo-Gangetic Plains (EIGP). Present study has intended to evaluate the consequences of salinity stress on selected drought tolerant rice genotypes at the most susceptible seedling stage with...
Chapter
Full-text available
Litchi (Litchi chinensis) is an important woody mycorrhizal fruit tree originated in China. In India, the agroclimatic conditions of foothills of the Himalayas in northern states like Bihar, West Bengal, Uttarakhand, Jharkhand, Punjab, and northeastern states such as Assam and Tripura provide immense scope for litchi cultivation. Various abiotic an...
Conference Paper
Full-text available
Red rice (Oryza longistaminata) is a perennial wild rice species with significant traits such as disease resistance and drought tolerance. Resistance genes could be transferred from O. longistamata to rice cultivars thereby contributing greatly to the improvement of rice crops. Despite these features, little is known about the molecular mechanisms...
Conference Paper
Full-text available
Rice is an important food crop consumed by majority of the world’s population but rice cultivation is severely impeded by submergence stress. However, submergence tolerance in rice varies with the occurrence or absence of the Sub1 gene. The present study envisioned to evaluate the consequences of submergence stress on near isogenic lines (NILs) of...
Conference Paper
Full-text available
The North East India can broadly be classified into four agro-climatic zones viz. humid continental severe winter, moist in all seasons and short summer (DFH); sub-tropical monsoon, mild and dry winter, warm and humid summer (CWB ); sub-tropical monsoon, mild and dry winter (CWA); and sub-tropical monsoon with very heavy rain (CWM). A PCR based stu...
Conference Paper
Full-text available
Mucuna pruriens commonly known as “Velvet bean” is an underutilized legume which is an important tropical, self-pollinating legume with various medicinal values. Determination of genetic diversity of this medicinal legume is important since the information will be beneficial to further breeding programmes. In the present study, 24 genotypes of Mucu...
Conference Paper
Full-text available
MicroRNAs (miRNAs) are small regulatory RNAs that regulate gene expression at the transcriptional or post-transcriptional level either through cleavage or translation inhibition of the target mRNA. Although a good number of miRNAs have been identified in various plants including Oryza sativa, no miRNA has been reported for Oryza minuta so far. O. m...
Conference Paper
Full-text available
Kiwifruit is one of the few new fruit crops that have become a high-value export crop for a number of countries. In North East Region (NER) of India, kiwifruit is mainly grown in Arunachal Pradesh which accounts for 97% production of total kiwifruit from NER of India. Kiwifruit is rich in Vitamin C, polyphenols as well as has strong anti-oxidant ef...
Conference Paper
Full-text available
microRNAs (miRNAs) are a class of small non-coding RNAs that play crucial role in regulating gene expression. The medicinal use of coffee based on its diuretic and stimulant property conferred by the effect of caffeine have been known since ancient time. The identification of miRNAs involved in regulating the biosynthesis of secondary metabolites i...
Article
Full-text available
The glyoxalase system catalyzes the conversion of cytotoxic methylglyoxal to d-lactate via the intermediate S-d-lactoylglutathione. It comprises two enzymes, Glyoxalase I (Gly I) and Glyoxalase II (Gly II), and reduced glutathione which acts as a cofactor by anchoring the substrates in the active sites of the two enzymes. The overexpression of both...
Article
Full-text available
MicroRNAs (miRNAs) are conserved, small non-coding endogenous RNAs,which are typically 21–24 nucleotides in length and play a pivotal role in post transcriptional gene regulation either by translational repression or cleavage. The regulation of target genes by miRNA has a central role in plant growth and development as well as in stress condition;...
Article
Full-text available
Introduction: MicroRNAs are small non protein coding entities that play crucial regulatory role in plants as well as animals by altering their gene expression either by wrecking or blocking the process of translation of the homologous mRNAs. It is an accepted fact that the plant miRNAs are conserved in nature and hence comparative genomics approach...
Conference Paper
Full-text available
Global issues are leading to concerns over food security. These include climate change, urbanization, increase in population subsequently leading to greater energy and water demand. Futuristic approach for crop improvement involves gene pyramiding for agronomic traits that empower the plants to withstand multiple stresses. In an earlier study from...
Conference Paper
Full-text available
The protein encoded by GmRLK18-1 (Glyma_18_02680 on chromosome 18) and GmRLK11-1 were nearly identical, syntenic, receptor like kinases (RLK) encoded within and outside the soybean (Glycine max L. Merr.) Rhg1/Rfs2 locus respectively. The locus underlies resistance to the soybean cyst nematode (SCN) Heterodera glycines (I.) and causal agent of sudde...
Conference Paper
Full-text available
MicroRNAs (miRNAs) are conserved, small non-coding endogenous RNAs, which are typically 21-24 nucleotide in length and plays a pivotal role in post transcriptional gene regulation either by translational repression or cleavage. In the present study, we report the computational identification of miRNAs and their targets from Expressed Sequence Tags...
Conference Paper
Full-text available
Soybean (Glycine max (L. Merr.)) resistance to any population of Heterodera glycines (I.), or Fusarium virguliforme (Akoi, O’Donnell, Homma & Lattanzi) required a functional allele at Rhg1/Rfs2. H. glycines, the soybean cyst nematode (SCN) was an ancient, endemic, pest of soybean whereas F. virguliforme causal agent of sudden death syndrome (SDS),...
Article
Full-text available
Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the β-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotyp...
Article
Full-text available
Carotenoid metabolism is regulated by several genes encoding carotenoid biosynthetic pathway enzymes. In the present study, a fruit transcriptome in tomato (Solanum lycopersicum) was compared between high lycopene accumulating genotype EC-521086 and low lycopene accumulating genotype VRT-32-1 at three different stages (green, breaker and red) of fr...
Data
Full-text available
Background The MYB gene family comprises one of the richest groups of transcription factors in plants. Plant MYB proteins are characterized by a highly conserved MYB DNA-binding domain. MYB proteins are classified into four major groups namely, 1R-MYB, 2R-MYB, 3R-MYB and 4R-MYB based on the number and position of MYB repeats. MYB transcription fact...
Data
Table S1. Nomenclature and classification of MYB TF family genes. Genome wide classification of MYB family genes including their characters such as GRAVY, PI, molecular weight and subcellular localization in rice and Arabidopsis.
Data
Table S6. Expression of MYB genes. Availability of full-length complementary DNA (FL-cDNA) / expressed sequence tag (EST) consequent to MYB genes.
Data
Table S7.MYB regulation under abiotic stress. Expression analysis of MYB genes under abiotic stress conditions in rice and Arabidopsis by using publically available microarray data.
Data
Table S9. Gene specific primers. List of gene specific primers used for QRT-PCR expression analysis of MYB genes in rice and Arabidopsis.
Data
Table S2. Functional assignment and subcellular localization of MYB TF family proteins. Molecular functional annotation of MYB TF family by gene ontology enrichment analysis including their subcellular localization in rice and Arabidopsis.
Data
Table S3. Sequence alignment of intronless MYB genes. Sequence comparison between rice and Arabidopsis intronless genes to predict conserveness.
Data
Figure S1.MYB gene expression under drought stress in rice. Analysis of MYB gene expression under drought stress in rice. We obtained MYB expression from our previously published microarray gene expression experiments [64].
Data
Figure S2.MYB gene expression under abiotic stresses in Arabidopsis. MYB gene expression under cold (a), drought (b) and salt (c) stresses in Arabidopsis. GENEVESTIGATOR database was used to analyze the MYB gene expression levels.
Data
Figure S5. Phylogenetic analysis of MYB proteins. Phylogenetic analysis of MYB proteins in both rice and Arabidopsis. The tree was constructed by using the multiple sequence alignment of bonafide MYB proteins.
Data
Table S4. Density of Introns. Distribution of introns in the MYB domain and other region of MYB genes in rice and Arabidopsis.
Data
Table S5. Diurnal/circadian expression. MYB expression under diurnal/circadian conditions in rice and Arabidopsis.
Data
Figure S3.MYB expression profiling using heatmap in Arabidopsis. Expression profile of MYB gene using heatmap for cold, drought, and salt stress, fetched by GENEVESTIGATOR database.
Data
Table S8. Tissues specific MYB expression. Tissue-specific expression profiling of MYB genes in rice and Arabidopsis.
Data
Figure S4.MYB expression profiles of different tissues in rice. Tissue specific expression profile of MYB gene in rice examine by MSU database.
Conference Paper
Full-text available
The amount of land available for crop production is decreasing steadily due to urban growth and land degradation. This decrease in the amount of arable land available for crop production and increase in human population will have major implications for food security over the next 2-3 decades. According to survey done by FAO in 2010, more than one b...
Conference Paper
Full-text available
The UID (Unique Identity) project is a scheme undertaken by the Government of India with the agenda of implementing the envisioned Unique Identification card (UID Card) to most of the population of India. Here, we propose the use of the mitochondrial DNA (mtDNA) D-loop hypervariable regions as an individual identification marker as DNA barcode in h...
Conference Paper
Full-text available
Tomato (Solanum lycopersicum) is an appropriate model system for studying the fruit ripening process because of the drastic color changes that occurs during different stages of fruit development. Carotenoids play an important role in biosynthesis of vitamin A, abscisic acid (ABA), and their ability to act as an excellent source of natural antioxida...
Conference Paper
Full-text available
Use of selectable marker genes that confer resistance to transgenic plants on selection media is an extra burden on plant genome and also makes transgenic plants unacceptable to the consumer. The present study was taken to construct a novel vector for introducing, the glyoxalase I (gly I) gene under an inducible promoter to develop marker free salt...

Network

Cited By

Projects

Project (1)
Project
Identification, characterization and expression profiling of drought stress responsive microRNAs in Coffea arabica L.