Raúl Domínguez-Perles

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• Doctor of Veterinary Medicine; Doctor of Science and Technology of Foods
• Researcher at Spanish National Research Council

The Douro Demarcated Region's winery industry produces million tonnes of by-products annually, making their reuse essential to minimize environmental impact. Grape stems, rich in bioactive compounds and nutrients, have demonstrated potential health benefits. This study analysed four red grape stems from the Douro region to assess their nutritional...

Background New circular economy policies for producing high‐value ingredients and co‐products, rich in bioactive phytochemicals, have boosted valorising agro‐food residues, as sources of powerful functional compounds. In this context, (poly)phenols of winemaking by‐products have been associated with anti‐inflammatory and oxidative stress (OS) preve...

Grape (Vitis vinifera L.) stems, a by-product of winemaking, possess significant potential value due to their rich polyphenolic composition, which allows their exploitation for cosmetic and pharmaceutical applications. This presents a promising opportunity for valorisation aimed at developing innovative products with potential health-promoting effe...

Lipophenols, phenolic compounds esterified with fatty alcohols or fatty acids, provide greater health benefits upon dietary ingestion of plant-based foods than unesterified (poly)phenols. Based on this premise, the present study aimed to demonstrate the role of gastrointestinal enzymes (pepsin, pancreatin, and pancreatic lipase) in releasing alkyl...

Resumen En el presente trabajo se compararon los efectos, sobre los compuestos fitoquímicos bioactivos y el perfil microbiológico, de las altas presiones hidrostáticas (HHP), como procesado no térmico y la pasteurización clásica (TP), como procesado térmico, en bebidas saludables de cítricos y maqui. Para ello se compararon los tratamientos de HHP...

Inflammatory diseases are strongly associated with global morbidity and mortality. Several mediators are involved in this process, including proinflammatory interleukins and cytokines produced by damaged tissues that, somehow, act as initiators of the autoreactive immune response. Bioactive compounds present in plant-based foods and byproducts have...

This study uncovered the impacts of microwave (MW) treatments compared to conventional pasteurization (TP) on the quality of functional citrus–maqui beverages, with added sucrose or stevia. The influence of these thermal treatments on the microbiological burden and phytochemical composition was determined by processing under two MW power levels (60...

The objective of this work is the development of a new fermented beverage (‘kombucha’-like), enriched with broccoli by-products as an ingredient, a source of organosulfur compounds, which could be biotransformed into more bioaccessible, bioavailable, and bioactive metabolites. The new beverages have shown variations in the physicochemical (pH, 3.6–...

Wine-making activity entails the production of solid and semisolid byproducts (grape stems and pomace and wine lees) that negatively impact the environment and industrial sustainability. Their features as sources of bioactive compounds support valorization procedures for functional and healthy ingredients. This work uncovers the quantitative alkyl...

The residues generated in the wine industry (pomace, stems, seeds, wine lees, and grapevine shoots) are a potential source of bioactive compounds that can be used in other industries despite being sometimes underestimated. Different extraction methods using various solvents and extraction conditions are currently being investigated. Due to its natu...

https://www.mdpi.com/journal/foods/special_issues/Phytochemicals_Inflammation_Oxidative

Prunus lusitanica L. is a shrub belonging to the genus Prunus L. (Rosaceae family) that produces small fruits with none known application. Thus, the aim of this study was to determine the phenolic profile and some health-promoting activities of hydroethanolic (HE) extracts obtained from P. lusitanica fruits, harvested from three different locations...

Los subproductos generados en la elaboración del vino constituyen un problema medioambiental y de gestión para las empresas. Este trabajo busca conseguir las condiciones idóneas, para conservar los compuestos bioactivos de dichos subproductos, con el fin de convertirlos en ingredientes funcionales. Así, se ha conseguido preservar el perfil fitoquím...

This study aims to take advantage of the wine industry by-products and extract bioactive compounds from grape pomace by applying methodologies susceptible to be integrated easily into industrial workflows because of the association with standard instrumentation and facilities, while the main factors affecting the efficiency of the process have been...

The amino acid tryptophan and its derived molecules serotonin and melatonin are involved in a wide range of physiological functions that contribute significantly to human health, namely antioxidant, immune-active, and neurological properties. Grapes and wine are a source of these compounds, but their presence in wine by-products remains underexplor...

In the last decade, most of the evidence on the clinical benefits of including cruciferous foods in the diet has been focused on the content of glucosinolates (GSL) and their corresponding isothiocyanates (ITC), and mercapturic acid pathway metabolites, based on their capacity to modulate clinical, biochemical, and molecular parameters. The present...

In recent times, less-known fruit species have increasingly attracted worldwide attention and their health benefits are at the forefront. The fruits of plants from the genus Prunus are good sources of nutrients due to their economic, agronomic, and healthy values. However, Prunus lusitanica L., commonly known as Portuguese laurel cherry is consider...

The present study aimed to discover the effects of exogenously foliar-applied salicylic acid (SA) in concentrations of 0, 1 and 15 mM (applied 7 and 21 days after heading) on oxidative stress. The effects were monitored through the concentrations of phytoprostanes (PhytoPs) and phytofurans (PhytoFs) in immature and mature grains of three genotypes...

The by-products of grapes (Vitis vinifera L.) in the winemaking process present a diverse phytochemical profile of (poly)phenols, essentially represented by phenolic acids, flavonoids, and stilbenes, which have health benefits. In winemaking, solid (grape stems and pomace) and semisolid (wine lees) by-products are generated, negatively impacting th...

Rocket salad is an important vegetable for the ready-to-eat sector, normally cultivated under greenhouse conditions, either in soil or soilless systems. In the latter case, as well as in the nurseries, its cultivation is usually carried out by using peat as a growing medium—a non-renewable substrate—for which it is urgent to find a replacement. Sim...

Broccoli is a highly consumed vegetable due to its content of bioactive nutrients and non-nutrients, including glucosinolates and isothiocyanates. Also, the consumption of craft beer has been boosted by the seek for enhanced sensory and functional properties. In this frame, broccoli have been proven as an ingredient to develop healthy beverages. Th...

Prunus lusitanica L., also known as Portuguese laurel or locally known as ‘azereiro’, is a rare species with ornamental and ecological value. Only two studies regarding the bioactivity and chemical composition of its leaves were reported to date. Thus, the present study aims to qualitatively and quantitatively evaluate the phenolic profile, through...

Recently, new formulations of beverages with low sugar and high bioactive compound contents are being demanded because of their association with metabolic health. However, the sweeteners’ influences on the bioactive compounds remain underexplored. In this sense, this work aims to evaluate the interactions between different bioactive compounds such...

The present study aims to discover the effect of exogenously applied of salicylic acid (SA) in the concentration 0, 1 and 15 mM, on the oxidative stress monitored through the concentration of PhytoPs and PhytoFs in immature and mature grains of three genotypes of rice ('R52', 'R45' and 'Yerua') and their influence on grains per panicle and chalkine...

Broccoli (Brassica oleracea var. italica) stalks account for up to 35% of the broccoli harvest remains with the concomitant generation of unused waste that needs recovery to contribute to the sustainability of the system. However, due to its phytochemical composition, rich in bioactive (poly)phenols and glucosinolates, as well as other nutrients, t...

Vegetables and fruits contain a variety of bioactive nutrients and non-nutrients that are associated with health promotion. Consumers currently demand foods with high contents of healthy compounds, as well as preserved natural taste and flavour, minimally processed without using artificial additives. Processing alternatives to be applied on plant-b...

The health benefits associated with (poly)phenols need to be supported by robust and insightful information on their biological effects. The use of in vitro, ex vivo, and in vivo models is crucial to demonstrate functionalities in specific targets. In this regard, bioaccessibility, bioavailability, and tissue/organ distribution need to be fully und...

Dear Colleagues, Research on bioactive compounds of plant origin is essential to understand the extent and mechanisms of action by which these phytochemicals provide desirable chemical and functional properties to plant-based foods. There is an increased awareness of the prophylactic effect of varied and balanced diets including a high proportion...

Cruciferous sprouts are rising in popularity as a hallmark of healthy diets, partially because of their phytochemical composition, characterized by the presence of flavonols and cinnamates. However, to shed light on their biological activity, the ability to assimilate (poly)phenols from sprouts (bioaccessible fraction) during gastrointestinal diges...

Fava bean (Vicia faba L.) yields are featured by high variability, influenced by the agro-environmental conditions during the growing seasons. These legume crops are sensitive to hydric and heat stresses. The adaptation depends on the efficiency of specific cultivars to use the available resources to produce biomass. This capacity is determined by...

Cruciferous vegetables are gaining importance as nutritious and sustainable foods, rich in phytochemical compounds such as glucosinolates (GSLs). However, the breakdown products of these sulfur-based compounds, mainly represented by isothiocyanates (ITC) and indoles, can contribute to human health. In the human digestive system, the formation of th...

The comparison of non-caloric sweeteners (stevia and sucralose) and sucrose, on the plasma concentration and cumulative effects of phenolic compounds, was achieved. A long-term intervention, consisting of the daily intake of 330 mL of healthy citrus-maqui soft drinks, for 60 days, by 138 healthy overweight adults, was followed. A total of 24 bioava...

Presently, the attention given to natural substances to counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such as extra virgin olive oil, as being powerful bioa...

Glucagon-like peptide (GLP)-1 and the glucose-dependent insulinotropic peptide (GIP) are incretin hormones that regulate the nutrient-stimulated insulin secretion from pancreatic β-cells. Their low plasma concentrations and rapid clearance pose certain methodological challenges for their detection and quantification. The currently available immunom...

The effect of coffee and cocoa on oxidative damage to macromolecules has been investigated in several studies, often with controversial results. This study aimed to investigate the effect of one-month consumption of different doses of coffee or cocoa-based products containing coffee on markers of DNA damage and lipid peroxidation in young healthy v...

BACKGROUND Juices are currently a fast‐growing segment in the fruit and vegetable industry sector. However, there are still no reports on the diversity of the phytochemical profile and health‐promoting properties of commercial sea buckthorn (Hippophaë rhamnoides) juices. This study aimed to identify and quantify phytoprostanes, phytofurans by ultra...

Cocoa and coffee are natural sources of phenolic compounds, which are degraded during beans processing of both. For this reason, there is an interest in obtaining extracts of these bio compounds. The aim of this study was uncovering the radical scavenging activity (AC) of mixtures of cocoa and coffee extracts, and the bioavailability of their pheno...

Nowadays, the health benefits associated with the consumption of plant-based food constitute a hot topic. To further demonstrate such benefits, related to antioxidant, anti-microbial, and anti-inflammatory activities, as well as the reduction of the risk of several pathophysiological conditions, the study of bioaccessibility and bioavailability of...

PurposeCoffee is an important source of bioactive compounds, including caffeine, trigonelline, and phenolic compounds. Several studies have highlighted the preventive effects of coffee consumption on major cardiometabolic (CM) diseases, but the impact of different coffee dosages on markers of CM risk in a real-life setting has not been fully unders...

Inflammation is a fundamental pathophysiological process which occurs in the course of several diseases. The present work describes the capacity of phytoprostanes (PhytoPs) and phytofurans (PhytoFs) (plant oxylipins), present in plant-based foods, to modulate inflammatory processes mediated by prostaglandins (PGs, human oxylipins) in lipopolysaccha...

PurposeSeveral studies have demonstrated the properties of hydroxytyrosol, a phenolic compound present in olive oils and olives with a well-characterized impact on human health. Nevertheless, some knowledge gaps remain on its bioavailability and metabolism; overall concerning to the real rate per cent of absorption and biovailability of dietary hyd...

Beer is a popular fermented beverage made, basically, by water, malted cereals, yeasts, and hops. However, the actual market demands innovative beers with new nutritional and organoleptic properties, achieved, for instance, by the addition of functional ingredients. In this hallmark, the objective of this work was to elaborate a new beer enriched w...

The intake of sugar-sweetened beverages has been associated with an augmented prevalence of metabolic diseases, namely, obesity, type II diabetes, and metabolic syndrome. On the other hand, nowadays, it is broadly accepted that foods and beverages rich in (poly)phenols could contribute to reducing the incidence of these pathologies. In this sense,...

Background and aim: The overconsumption of sucrose is closely related to sugar-sweetened beverages and one of the main factors associated with the increase of metabolic diseases, such as type 2 diabetes, obesity, and insulin resistance. So, the addition of alternative sweeteners to new fruit-based drinks could contribute to minimizing the incidenc...

Water scarcity is a threat for food production because, water, is more and more limited and force farmers to use new deficit irrigation (DI) strategies without affecting fruit yield and quality. No information exists on almond polyphenols and proanthocyanidins (PAs) produced under DI. The present work studied the effect of 2 regulated DI (RDI) and...

Even though traditionally date-fruit has been featured by a marginal use, mainly restricted to its dietary intake, in recent years, it has raised the range of applications for this agro-food production.. These new uses have entailed an enlarged production of date fruits and, simultaneously, of date palm by-products. Encouraged by the traditional me...

Pistachio (Pistacia vera L.) is a drought-tolerant specie grown under the semiarid conditions of the Mediterranean basin. For this reason, it is essential to make an exhaustive quantification of yield and quality benefits of the kernels because the regulated deficit irrigation will allow significant water savings with a minimum impact on yield, whi...

Lipophenols have been stressed as an emerging class of functional compounds. However, little is known about their diversity. Thus, this study is aimed at developing a new method for the extraction, clean-up, and UHPLC-ESI-QqQ-MS/MS-based analysis of the lipophenols derived from hydroxytyrosol (HT): α-linolenic (HT-ALA), linoleic acid (HT-LA), and o...

Moringa oleifera L. was grown under cadmium and lead stress conditions and the variations in its mineral content, polyphenolics, and antioxidant activities were studied and how these heavy metals affect plant growth and development. In this study, the metal translocation factor was found <1 which indicates more metal accumulation in moringa roots t...

Coffee is an important source of bioactive compounds, including caffeine, trigonelline, and phenolic compounds. Several studies have highlighted the preventive effects of coffee consumption on major cardiometabolic diseases, but the impact of coffee dosage on markers of cardiometabolic risk is not well understood. Moreover, the pool of coffee-deriv...

Almonds have gained consumers attention due to their health benefits (they are rich in bioactive compounds) and sensory properties. Nevertheless, information about phytoprostanes (PhytoPs) and phytofurans (PhytoFs) (new plant markers of oxidative stress and compounds with biological properties for human health) in almonds under deficit irrigation i...

Background: An array of bioactive compounds with health-promoting effects has been described in several species of macroalgae. Among them, phytoprostanes (PhytoPs) and phytofurans (PhytoFs), both autoxidation products of α-linolenic acid, have been seen to exert immunomodulatory and antiinflammatory activities in vitro. The purpose of this study w...

Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are plant oxylipins (isoprostane-prostaglandin-like compounds) formed by the free radical-catalyzed oxidation of α-linolenic acid (ALA, C18:3, n-3). These oxylipins have been previously described in an array of plant-based foods with similar concentrations as phenolics in some fruits and vegetables...

Nowadays, there is a gap between the theoretical bioactivity of (poly)phenols and their real influence in health, once ingested. Due to this, new studies, including in vitro and in vivo models that allow for exploring bioaccessibility, bioavailability, and bioactivity, need to be developed to understand the actual importance of consuming functional...

Sweet chestnut (Castanea sativa Miller) is a tree species whose edible fruit was a staple food for many centuries until the emergence of potato and cereal crops. Once of great importance for the survival of rural populations, at present there is a renewed interest in this fruit as a nutritious food, partly due being gluten-free. Portugal is one of...

During the last two decades we have witnessed a change in the concept of health. Nowadays, being healthy is much more than just not being ill. In this sense, a new trend has been installed in relation to wellbeing and health, which focuses mainly on food, nutrition, and physical exercise (sport). All this has led to the development of new foods (ma...

Centaurium erythraea Rafn is a food flavouring used in both food and beverage industries. Despite the existence of some works on this species, only few focus their attention on its common form of consumption (infusion). Thus, we intended to explore the chemical composition and the biological properties of infusions from C. erythraea aerial parts. P...

Given the growing interest in phytoprostanes (PhytoPs) and phytofurans (PhytoFs) in the field of plant physiology, biotechnology, and biological functions, the present study aimed to optimize a method of enzymatic hydrolysis which utilizes bacterial and yeast esterases that allow the appropriate quantification of PhytoPs and PhytoFs. To obtain the...

The consumption of sugar-sweetened beverages has been related with the risk of cardiovascular diseases and other pathophysiological situations, such as obesity or diabetes mellitus. Given the increasing awareness on this fact, food industries are developing new products to reduce the amount of added sugar in development of food products development...

Sugar intake abuse is directly related with the increase of metabolic diseases such as type 2 diabetes, obesity, and insulin resistance. Along this line, the development of new beverages using alternative sweeteners could help with combatting the pathophysiological disorders associated to the consumption of sugar. To provide evidence on this issue,...

Dietary sugar has been largely related to the onset of metabolic diseases such as type 2 diabetes and obesity, among others. The growing awareness on the close relationship between the dietary habits and this health disturbance has encouraged the development of new beverages using alternative sweeteners that could contribute to combat the above ref...

Response surface methodology (RSM) was chosen to optimize the influence of solvent pH and relative proportion, and time of extraction, regarding polyphenols and radical scavenging capacity of almond (Prunus dulcis (Mill.) D.A. Webb) by-products (hulls, shells, and skins) from an almond orchard located in the North of Portugal (Lousa, Torre de Monco...

Bioactive phytoprostanes and phytofurans are synthesized in higher plants by non‐enzymatic oxidation of α‐linolenic acid (C18:3 n‐3), triggered by high concentrations of reactive oxygen species. In the current scenario of changing dietary patterns, additional information is needed on the concentrations of oxylipins in legumes and on the effect of s...

Almond (Prunus dulcis (Mill.) D. A. Webb) production keeps an increasing trend worldwide, leading to augment in generation of harmful by-products that should be valorized as a source of bioactive phytochemicals with application in the development of new added-value products. The assessment of almond hulls and skins on their (poly)phenolic compositi...

Roasted cotyledons of the Chilean hazelnut (Gevuina avellana) are appreciated as snacks. The aim of our work was to assess the fatty acid, oxylipin and phenolic composition using gas chromatography (GC) coupled to mass spectrometry (MS), ultra- high performance liquid chromatography (UHPLC) coupled to MS and HPLC coupled to diode array detector (HP...

Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) occur in higher plants as a result of the non-enzymatic oxidation of α-linolenic acid (ALA, C18:3 n-3), triggered by increased levels of Reactive Oxygen Species in response to biotic and abiotic stress that can constitute relevant biomarkers of oxidative stress. Currently, in the scenario of growin...

A new method using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) methodology was developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) contents in liquid and solid vegetable samples. The advantages of this method are speed, high sensitivity and practic...

Recent years have witnessed tremendous advances in food science, including improvements in industrial process technology and control systems, improvements in farming systems for growing and harvesting food, genetic improvements to organisms used in the food supply, and improvements in techniques to monitor food safety and nutritional quality. It is...

Rice plays a key role in the food security of more than half of the world's population. At least 60% of cultivated soils have abiotic stress limitations caused by mineral deficiencies, temperatures, extremes, drought or acidity. Specific technological practices can help to reduce the damage caused. Phytoprostanes and phytofurans (PhytoPs and PhytoF...

The scientific background on the perinatal pathology, regarding both mother and offspring, from the perspective of lipidomics has noticed the chance of identifying new promising clinical markers of oxidative stress and inflammation, closely related with the normal development of unborn and new-born child that gives rise to new and promising perspec...

Dear Colleagues, The functional compounds in foods and by-products have attracted the attention of the scientific community because of the requirement of finding and controlling the factors that contribute to lessening the prevalence and severity of certain diseases and aging. In this connection, the bioactive constituents of foods and, especiall...

Wild animals may be considered important reservoirs for bacterial pathogens and, consequently, possible sources of infection for humans. In this study, selected multidrug-resistant bacteria (Acinetobacter spp., Aeromonas salmonicida, Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from wild animals were characte...

This Special Issue is intended to cover the existing gapbetween classical studies and the occurrence of new typesof (poly)phenol derivatives (e.g., lipophenols) that havebeen scarcely explored to-date. We are also interested inthe influence of the digestive process on thebioaccessibility and bioavailability of these compoundsand the advantages of n...

Edible sprouts with germinating seeds of a few days of age are naturally rich in nutrients and other bioactive compounds. Among them, the cruciferous (Brassicaceae) sprouts stand out due to their high contents of glucosinolates (GLSs) and phenolic compounds. In order to obtain sprouts enriched in these phytochemicals, elicitation is being increasin...

HPLC-PDA-ESI-MS n analysis of different parts such as stem bark, shoot, flower, fruit and root of Capparis spinosa (C. spinosa) and Capparis decidua (C. decidua), collected in rainy and dry seasons from the Cholistan desert of Pakistan, depicted the occurrence of a wide array of phenolics with quercetin, apigenin and kaempferol derivatives along wi...

In rice crops, fertilization is a naturalized practice, although inefficient, that could be improved by applying foliar fertilization. Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are products of α-linolenic acid peroxidation, useful as biomarkers of oxidative degradation in higher plants. The objective was to determine the effect of the foli...

Centaurium erythraea Rafin. is a food flavouring used in both food and beverage industries. Despite the existence of some works on this species, only few of them focus their attention on its common form of consumption (infusion). Thus, we intended to explore the chemical composition and the biological properties of infusions from C. erythraea aeria...

Structure-activity relationship (SAR) constitutes a crucial topic to discover new bioactive molecules. This approach initiates with the comparison of a target candidate with a molecule or a collection of molecules and their attributed biological functions to shed some light in the details of one or more SARs and subsequently using that information...

Structure-activity relationship (SAR) constitutes a crucial topic to discover new bioactive molecules. This approach initiates with the comparison of a target candidate with a molecule or a collection of molecules and their attributed biological functions to shed some light in the details of one or more SARs and subsequently using that information...