Raúl de la Puente

Raúl de la Puente
Universidad de León | UNILEON · Department of Animal Health

Ph.D. in Molecular Biology

About

8
Publications
1,215
Reads
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53
Citations
Education
August 2006 - May 2012
Universidad de León
Field of study
  • Molecular Biology and Biotechnology -Genetics
May 2003 - April 2005
Universidad de León
Field of study
  • Genetics
September 1998 - September 2003
Universidad de León
Field of study
  • Biology

Publications

Publications (8)
Article
Full-text available
Objective: Peripheral blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because the presence of disease and prognostic information is reflected in the gene expression pattern. Quality RNA is used by a number of different downstream applications, so the selection of the most appropriate RNA stabilizati...
Article
Full-text available
A previous Lens genetic map was improved by adding 31 molecular genetic markers, reaching a total of 190 markers with undistorted segregation. Data were obtained from the segregational analysis of 113 F2 plants generated from a single hybrid of Lens culinaris ssp. culinaris × L. c. ssp. orientalis. The added markers are predominantly codominant (15...
Article
Full-text available
A previous Lens genetic map was improved by adding 31 molecular genetic markers, reaching a total of 190 markers with undistorted segregation. Data were obtained from the segregational analysis of 113 F2 plants generated from a single hybrid of Lens culinaris ssp. culinaris × L. c. ssp. orientalis. The added markers are predominantly codominant (15...
Article
Amplified fragment length polymorphism (AFLP) analysis of 24 in vitro regenerated rye plants was performed in order to evaluate the somaclonal variation rate in this species and to identify rye genomic regions where mutations are preferentially promoted by in vitro culture processes. Regenerated plants were obtained from cell lines derived from imm...
Article
Full-text available
The available methods to isolate specific amplified fragment length polymorphism (AFLP) markers can be used only if markers are detected by radioactive labeling, silver staining, or ethidium bromide staining; these methods are useless if modern and automated genetic analyzers are used to detect AFLP markers by fluorescent labeling. We have develope...

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