Rashmi Kulkarni

Rashmi Kulkarni
Indian Institute of Science Education and Research, Pune | IISER · Department of Biology

Pursuing PhD

About

3
Publications
30,329
Reads
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20
Citations
Citations since 2017
0 Research Items
12 Citations
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201720182019202020212022202301234
Additional affiliations
August 2010 - present
Indian Institute of Science Education and Research, Pune
Position
  • PhD Student
Description
  • My PhD work is related to computational biology of type 2 diabetes; specifically centered on defining personalized glucose targets using oxidative stress molecule- glutathione.

Publications

Publications (3)
Article
Full-text available
Cellular and animal studies suggest that oxidative stress could be the central defect underlying both beta-cell dysfunction and insulin resistance in type 2 diabetes mellitus. A reduction of glycemic stress in diabetic patients on therapy alleviates systemic oxidative stress and improves insulin resistance and beta-cell secretion. Monitoring oxidat...
Conference Paper
Oxidative stress (OS) is a central causal locus through which hyperglycemia leads to post-diabetic complications. Using a mechanistically derived minimal mathematical model, we showed that steady state OS and glycemic state (GS) in newly diagnosed type 2 diabetics can be fitted well using a Goldbeter-Koshland function (GK) [Kulkarni R et al., PLoS...
Article
Full-text available
Excess glucose – hyperglycemia – has long been associated with type 2 diabetes. Ancient literature from Egypt and India describe the disease; it was easily identifiable because “a patient’s urine attracted ants” (1). More than two millennia later, monitoring glycemic status continues to be central to clinical management. It is currently the only va...

Questions

Questions (3)
Question
I am growing myotubes in 96 well plate for treatment with free fatty acid. However, they come off after washing or even treatment with some dye for 15-20 minutes in a buffer. This is with myotubes without treatment as well. It was not happening earlier. I observed that cell crowding at the edge of the well might be the issue, so instead of 4th day I started using 3rd day old myotubes, but still there is no change in the behavior. I am wondering how I can control this? Do you have any help?
Question
I am treating C2C1 myotubes with free fatty acid and would like to measure mitochondrial specific super-oxide production. I will be using MitoTEMPO for specificity. I have couple of questions regarding MitoTEMPO treatment. How long MitoTEMPO treatment should be given (I didn't get much information about this, information about the concentration is mentioned)? Secondly, I found that in some references MitoTEMPO is added along with the stress inducer and MitoSox at the final hour of the stress inducer treatment NOT after completion of the treatment. What is right way to do it ?? Any suggestions/ideas/references on this ? Thanks !

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