Rashed Alghafri

Rashed Alghafri
International Centre for Forensic Science · Research and Development

Doctor of Philosophy

About

41
Publications
6,094
Reads
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299
Citations
Introduction
Additional affiliations
September 2020 - present
Murdoch University
Position
  • Professor
September 2019 - present
Bond University
Position
  • Professor (Associate)
Description
  • Supervising Research Students
October 2015 - present
United Arab Emirates University
Position
  • Professor (Assistant)
Education
October 2011 - December 2014
University of Central Lancashire
Field of study
  • Forensic Genetics
January 2009 - December 2009
Bond University
Field of study
  • Forensic Genetics
February 2005 - December 2008
Flinders University
Field of study
  • Forensic and Analytical Chemistry

Publications

Publications (41)
Article
Full-text available
Background: As high touch wearable devices, the potential for microbial contamination of smart watches is high. In this study, microbial contamination of smart watches of healthcare workers (HCWs) was assessed and compared to the individual's mobile phone and hands. Methods: This study was part of a larger point prevalence survey of microbial co...
Article
Full-text available
This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in differentiating closely related males. The multiplex pe...
Article
Full-text available
Advancements in technology and communication have revolutionised the twenty-first century with the introduction of mobile phones and smartphones. These phones are known to be platforms harbouring microbes with recent research shedding light on the abundance and broad spectrum of organisms they harbour. Mobile phone use in the community and in profe...
Article
Full-text available
Background: Mobile phones of healthcare workers (HCWs) can act as fomites in the dissemination of microbes. This study was carried out to investigate microbial contamination of mobile phones of HCWs and environmental samples from the hospital unit using a combination of phenotypic and molecular methods. Methods: This point prevalence survey was ca...
Preprint
Full-text available
Introduction. Advancements in technology and communication have revolutionised the 21st century with the introduction of mobile phones and smartphones. These phones are known to be platforms harbouring microbes with recent research shedding light on the abundance and broad spectrum of organisms they harbour. Mobile phone use in the community and in...
Article
Full-text available
The Hazara population across Durand line has experienced extensive interaction with Central Asian and East Asian populations. Hazara individuals have typical Mongolian facial appearances and they called themselves descendants of Genghis Khan’s army. The people who speak the Balochi language are called Baloch. Previously, a worldwide analysis of Y-c...
Article
Full-text available
There is increasing attention focussed on the risks associated with mobile phones possibly serving as ‘Trojan Horse’ fomites for microbial transmission in healthcare settings. However, little is reported on the presence of microbes on community derived mobile phones which in 2021, numbered in the billions in circulation with majority being used on...
Article
Full-text available
An ever-increasing number of medical staff use mobile phones as a work aid, yet this may pose nosocomial diseases. To assess and report via a survey the handling practices and the use of phones by paediatric wards healthcare workers. 165 paediatric healthcare workers and staff filled in a questionnaire consisting of 14 questions (including categori...
Article
Full-text available
Introduction Mobile phones are used the world over, including in healthcare settings. This study aimed to investigate the viable microbial colonisation of mobile phones used by healthcare personnel. Methods Swabs collected on the same day from 30 mobile phones belonging to healthcare workers from three separate paediatric wards of an Australian ho...
Chapter
The application of the Y chromosome short tandem repeats (Y-STR) in forensic field and lineage studies has been dramatically growing in the last few decades. In forensic cases, when male–female mixtures cannot be resolved using autosomal STR multiplexes kits, the application of Y-STR profiling will add a beneficial value by producing the male Y-STR...
Preprint
Full-text available
Hazara population across Durand- line has experienced extensive interaction with Central Asian and East Asian populations. Hazara individuals have typical Mongolian facial appearances and they called themselves descendants of Genghis Khan’s army. The people who speak the Balochi language are called Baloch. Previously, a worldwide analysis of Y-chro...
Article
Severe Acute Respiratory Syndrome – Coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China and spread to more than 114 countries resulting in a pandemic, which was declared by the WHO in March 2020. Tracking the spread of the virus raised a main concern in every country. Many researches proved the presence of SARS-CoV-2 in stool samples of patients, wh...
Article
Full-text available
Get a proper chair, don't eat at your desk, and no phones in the loo-how to keep your home workspace safe and hygienic https://theconversation.com/get-a-proper-chair-dont-eat-at-your-desk-and-no-phones-in-the-loo-how-to-keep-your-home-workspace-safe-and-hygienic-143535
Article
Background Mobile phones have become an integral part of modern society. As possible breeding grounds for microbial organisms, these constitute a potential global public health risk for microbial transmission. Objective Scoping review of literature examining microbial's presence on mobile phones in both health care (HC) and community settings. Me...
Article
The recently introduced 6-dyes Yfiler® Plus multiplex kit which includes 27 Y-STR loci has been used to study 436 UAE Arab male individuals. In addition to the 17 Y-STRs traditionally used in Yfiler® multiplex kit, Yfiler® Plus has ten additional Y-STRs. It includes seven rapidly mutating Y-STRs (RM Y-STRs) namely DYS560, DYS576, DYS627, DYS518, DY...
Article
In the present study, forensic parameters were estimated for three populations residing in the United Arab Emirates (UAE) including UAE Arabs, Pakistanis and Indians based on the population data of 23 autosomal short tandem repeats (STRs). The UAE Arabs is a vital population to study due to high rates of consanguineous marriages. Therefore, it is e...
Article
Mitochondrial DNA analysis has earned its place in the field of genetics for providing a new window into understanding population ancestry. Its ability to produce results from minimal or decayed samples where nucleus DNA profiling proves are difficult is an additional benefit to forensic genetics. A total of 150 whole blood samples were collected o...
Article
Importance of rapidly mutating (RM) Y- STRs) is already established and is evident with the addition of seven RM Y-STR markers in new generation Yfiler Plus (YFP) multiplex system. Performance of the 7 RM Y-STRs included in YFP is evaluated in central Indian population. We also report here a pilot study on genetic poly-morphism using 13 Rapidly Mut...
Article
Y-chromosomal Short Tandem Repeats have been widely used in forensic investigations, identification of males for criminal justice purpose and population genetics. Commercially available Y-STRs kits allow the identification of male pedigrees and has a limited application in forensic genetics because of its limitation in differentiating closely relat...
Article
Autosomal short tandem repeats (STRs) have been widely used in forensic investigations. Prior to the application of any DNA based identification method, it is essential to estimate the allele frequencies and forensic statistical parameters of targeted STR loci in each population in order to provide a more precise reference database for forensic inv...
Article
Since the beginning of our work in 2003 our laboratory has focused exclusively on STR DNA from bone, a powerful tool in missing person cases. In cases such as mass disasters or missing persons, human remains are challenging to identify as they may be fragmented, burnt, recovered from water, degraded, and/or contain inhibitory substances. To address...
Article
The effectiveness of a rapidly mutating Y-STRs multiplex assay (RM Y-STRs) containing 13 RM Y-STR markers was compared with the AmpFℓSTR™ Yfiler™ PCR Amplification Kit (Yfiler) with conventional Y-STR markers for their efficiency in differentiating males within the same paternal lineage. Samples from 4 generations comprising 16 Dravidian males (15...
Article
An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the order of 200 rfu for major contributors) five participant...
Article
FTA® cards were developed for analysing reference samples such as blood or saliva and helping to store the sample for long term purposes as it kills the microorganisms that comes along with biological stains which can be potential inhibitors. The aim of this research is to optimize a reduced volume PCR amplification for STR analysis of reference bl...
Article
To assess our laboratory's success with skeletal remains and provide a benchmark for the forensic community involved in identification of these remains, we retrospectively examined our ability to develop DNA profiles from the remains analyzed in our laboratory in the last 7 years. Between January 2009 and December 2016, 70 DNA extractions were comp...
Article
Y chromosome short tandem repeats (Y-STR) has been widely used in forensic DNA profiling and lineages analysis. The aim of this study is to establish a database of an extensive Y-STR profiles of the United Arab Emirates (UAE) population and to investigate the distribution of 17 Y-STR loci. Samples of 345 Emirati males using 17 Y-STR loci included i...
Article
Recently, the interest of the forensic community has been focused on new Y-chromosomal short tandem repeats (Y-STRs), termed Rapidly Mutating Y-STRs (RM-YSTRs), which is able to differentiate between close males belonging to the same paternal lineage due to their high mutation rates. In this study, we have estimated a mutation rate for 13 RM-YSTR i...
Article
Recent research with Rapidly Mutating Y-STRs (RM Y-STRs) have shown that these loci provide substantially higher haplotype diversity and haplotype discrimination capacity in worldwide populations when compared with the Y-STRs commonly used in genetic forensics. The aim of this study was to develop an allelic frequency database for the population of...
Article
Full-text available
Rapidly mutating Y-chromosomal short tandem repeats (RM Y-STRs) were identified to improve differentiation of unrelated males and also to enable separating closely and distantly related males in human identity testing in forensic and other applications. RM-Yplex assay was developed as a single multiplex that is capable of simultaneously amplifying...
Article
Full-text available
A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum(®) Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multip...
Article
Multi-allelic Y chromosome short tandem repeats (Y-STRs) have been avoided in forensic applications due to high level of complexity involved in the interpretation of these markers. Because of such reason Y-STR multi-allelic markers have less representation in literature compared to Y-STR single-allelic markers. However these markers were proven to...
Article
Rapidly Mutating Y-STRs (RM Y-STR) were proven to have significantly higher resolution in worldwide populations when compared to commonly used Y-STR kits. In order to contribute to the research that has been conducted so far, RM Y-STRs were investigated in parallel with Yfiler® kit in 327 male individuals from United Arab Emirates population. Such...
Article
The high power of discrimination for SE33 locus makes it one of the most informative loci for forensic applications. Considering that locus finding its way to most of the new commercially available autosomal STR multiplexes assays, it is important to evaluate forensic parameters in UAE population before implementing kits including such locus. Here...
Article
The analysis of mini short tandem repeats (miniSTRs) is useful in various contexts of forensic DNA analysis. Main application is the identification of DNA profiles from degraded DNA samples. In the present study, previously identified autosomal miniSTR markers were incorporated in single multiplex assay in order to evaluate the use of such markers...

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Projects

Project (1)
Project
1: Study of Y - Chromosomes variations 2: Study of X - Chromosomes Variations 3: Mitotyping 4: EVCs etc