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Raquel Iglesias-Fernández

Raquel Iglesias-Fernández
Centro de Biotecnología y Genómica de Plantas (CBGP)-Universidad Politécnica de Madrid · Plant Biology-Biotechnology

PhD
I am interested in molecular and genetic mechanisms behind seed maturation and germination in model species and crops.

About

35
Publications
7,032
Reads
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850
Citations
Introduction
My main research interest is transcriptional control of gene expression, mainly in seed maturation, dormancy, and germination in model species (Arabidospis and Brachypodium distachyon) and crops (Barley and Chenopodium quinoa). I am also interested in studying transcription factors affecting metabolic adjustment to carbon/nitrogen ratio.
Additional affiliations
August 2021 - present
Escuela Técnica Superior de Ingeniería Agronómica Alimentaria y de Biosistemas (ETSIAAB)- Universidad Politécnica de Madrid (UPM)
Position
  • Professor (Assistant)
Education
May 2007 - December 2007
Wageningen University & Research
Field of study
  • Seed molecular biology
September 2004 - July 2009
University of Santiago de Compostela
Field of study
  • Seed Physiology
September 1999 - July 2004
University of Santiago de Compostela
Field of study
  • Biology (Molecular Biology orientation)

Publications

Publications (35)
Article
Full-text available
The seed is the propagule of higher plants and allows its dissemination and the survival of the species. Seed dormancy prevents premature germination under favourable conditions. Dormant seeds are only able to germinate in a narrow range of conditions. During after-ripening (AR), a mechanism of dormancy release, seeds gradually lose dormancy throug...
Article
Full-text available
The barley endo-β-mannanase (MAN) gene family (HvMAN1-6) has been identified and the expression of its members analyzed throughout different plant organs, and upon grain development and germination. The HvMAN1 gene has been found to be highly expressed in developing and germinating grains. The MAN (EC 3.2.1.78) enzymatic activity gets a maximum in...
Article
Full-text available
Main conclusion: Mannans but not endo-β-mannanases are mainly found in the mucilage layer of two Brassicaceae seeds. Nonetheless, mannanase mobilization from inner to outer seed layers cannot be ruled out. The contribution of endo-β-mannanase (MAN) genes to the germination of the wild-type Sisymbrium officinale and cultivated Brassica rapa (Brassi...
Article
Full-text available
Mitochondrial thioredoxin-o (AtTrxo1) was characterized and its expression examined in different organs of Arabidopsis thaliana. AtTrxo1 transcript levels were particularly high in dry seeds and cotyledons where they reached a maximum 36 h after imbibition with water, coinciding with 50% germination. Expression was lower in seeds germinating in 100...
Article
Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusivel...
Article
Seed development follows zygotic embryogenesis; during the maturation phase reserves accumulate and desiccation tolerance is acquired. This is tightly regulated at the transcriptional level and the AFL (ABI3/FUS3/LEC2) subfamily of B3 transcription factors (TFs) play a central role. They alter hormone biosynthesis, mainly in regards to abscisic aci...
Chapter
Full-text available
Flooding and submergence are two stresses leading to anoxic conditions in the root system. Under this stress both anoxia and hypoxia are defined by O2 shortage. Flooding negatively prevents O2 influx into the root system, thus reducing oxidative phosphorylation and ATP levels. Flood tolerant higher plant species have adequate plasticity to adjust t...
Article
Accumulation of storage compounds in the starchy endosperm of developing cereal seeds is highly regulated at the transcriptional level. These compounds, mainly starch and proteins, are hydrolyzed upon germination to allow seedling growth. In barley seed development, the transcription factor HvGAMYB is a master activator both in the maturation and g...
Article
Full-text available
Immunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysaccharides in the root embryo and in the coleorhiza in the early stages of germination (12h), decreasing thereafter to the point of being hardly detected at 27h. Concurrently, the activity of endo-β-mannanases (MANs; EC 3.2.1.78) that catalyse t...
Article
Full-text available
Despite evolutionary conserved mechanisms to silence transposable element activity, there are drastic differences in the abundance of transposable elements even among closely related plant species. We conducted a de novo assembly for the 375 Mb genome of the perennial model plant, Arabis alpina. Analysing this genome revealed long-lasting and recen...
Article
Full-text available
Gibberellins (GAs) are plant hormones that affect plant growth and regulate gene expression differentially across tissues. To study the molecular mechanisms underlying GA signaling in Arabidopsis thaliana, we focused on a GDSL lipase gene (LIP1) induced by GA and repressed by DELLA proteins. LIP1 contains an L1 box promoter sequence, conserved in t...
Article
Main conclusion BdDOF24 interacting with BdGAMYB regulates the BdCathB gene upon germination. During barley seed germination, hydrolytic enzymes (α-amylases, proteases, etc.) synthesized in the aleurone layer in response to gibberellins (GA), catalyse the mobilization of storage reserves accumulated in the endosperm during seed maturation. In Bra...
Article
Full-text available
Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA ins...
Article
Endo-β-mannanases (MAN; EC. 3.2.1.78) catalyze the cleavage of β1→4 bonds in mannan-polymers and have been associated with the weakening process of the embryo surrounding tissues during seed germination. In Arabidopsis thaliana germinating seeds, the most highly expressed MAN gene is AtMAN7 and its transcripts are restricted to the micropylar endos...
Article
We have here demonstrated for the first time that nitrate not only accelerates testa rupture of non-AR seeds but also modifies expression pattern of the cell-wall remodeling proteins (mannanases; SoMAN6 and SoMAN7) and key genes belonging to metabolism and signaling of ABA (SoNCED6, SoNCED9, SoCYP707A2 and SoABI5) and GAs (SoGA3ox, SoGA20ox, SoGA2o...
Article
Full-text available
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the predominant hemicellulosic CW polymers in many genera of green algae. The e...
Article
Full-text available
The transition from the dormant to the non-dormant state of a viable and mature seed can take place at low hydration by exposure to air-dry storage conditions (dry afterripening; AR). The events occurring during this loss of dormancy are of considerable physiological, ecological and agricultural interest. AR may be attributable to increased sensiti...
Article
Full-text available
The endo-β-mannanase (MAN) family is represented in the Arabidopsis genome by eight members, all with canonical signal peptides and only half of them being expressed in germinating seeds. The transcripts of these genes were localized in the radicle and micropylar endosperm (ME) before radicle protrusion and this expression disappears as soon as the...
Article
Full-text available
Mannans are hemicellulosic polysaccharides in the plant primary cell wall (CW). Mature seeds, specially their endosperm cells, have CWs rich in mannan-based polymers that confer a strong mechanical resistance for the radicle protrusion upon germination. The rupture of the seed coat and endosperm are two sequential events during the germination of A...
Article
Full-text available
The rupture of the seed coat and that of the endosperm were found to be two sequential events in the germination of Sisymbrium officinale L. seeds, and radicle protrusion did not occur exactly in the micropylar area but in the neighboring zone. The germination patterns were similar both in the presence of gibberellins (GA(4+7)) and in presence of e...
Article
Full-text available
After-ripening (AR) in Sisymbrium officinale seeds altered SoACS7, SoACO2, SoGA20ox2, SoGA3ox2, and SoGA2ox6 gene expression. Except for SoGA20ox2 expression, which sharply diminished, the expression of the other genes rose during development, particularly that of SoACS7. In contrast, only the SoACO2 and SoGA2ox6 transcripts increased with seed des...
Article
Full-text available
Seed dormancy is a primary component of the different life-history strategies of annual plants. The evolution of the seed habit has permitted higher plants to colonize a large number of ecologicla niches due to the excellence of ortodox seeds as dispersal units relatively impervious to harsh environmental conditions. Seed dormancy, defined as the i...
Article
In this work, a complete cDNA encoding a polygalacturonase (Pd-PG1) has been identified by screening a cDNA library constructed from the ripe mesocarp of Prunus domestica L. subsp. insititia (damson plum). According to similarity in the amino-acid sequence and the absence of pro-sequence, Pd-PG1 belongs to a group of PGs that are expressed in fruit...
Article
In this work, four cDNA clones (Pd-ACS1,AJ890088; Pd-ETR1 and Pd-ERS1, AJ890092, AJ890091; and Pd-CTR1, AJ890089) encoding an ACC-synthase, two putative ethylene (ET) receptors, and a putative MAPKKK, respectively, were isolated and phylogenetically characterized in Prunus domestica L. subsp. insititia. Their expression was studied by real-time PCR...
Article
Full-text available
The seeds of Sisymbrium officinale are largely heterogeneous in mass and colour within the ripe fruit. In order to initiate the charac-terization of the germinative process from these heterogeneous seeds, we have used two different seed lots (i.e. brown and light-brown), and studied several physical and physiological properties and their possible i...

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