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Introduction
Current institution
Current position
- Senior Researcher
Additional affiliations
September 1993 - present
Education
September 1996 - May 2001
Publications
Publications (111)
Ticks, hematophagous ectoparasites, pose significant health risks to wildlife and humans as vectors for various pathogens. This study aims to assess the efficacy of Ailanthus altissima plant extract against Rhipicephalus (Boophilus) microplus and Hyalomma anatolicum ticks using both in vitro and in silico approaches. According to gas chromatography...
Ticks are haematophagous ectoparasite of mammals, birds and reptiles, distributed worldwide and have significant medical and veterinary importance due to the direct damage they cause to their hosts and as vectors of human and animal pathogens. They are among the most competent and versatile vectors of pathogens and are second only to mosquitoes in...
Infections by the Rhipicephalus microplus tick represent a serious health problem in the global livestock industry, mainly due to direct damage and the vector’s capacity to transmit various pathogens. Currently, tick infections have been increasing due to the emergence of populations resistant to ixodicides. Therefore, the search for new targets th...
The tick Rhipicephalus microplus and the diseases it causes, such as anaplasmosis and babesiosis, are critical factors that severely impact cattle production and related industries globally. The prevalence of tick infestations in livestock has increased due to the increasing resistance of ticks to pesticides. Evidence shows that specific genes and...
Rhipicephalus microplus is a blood-sucking parasite that causes heavy infestations on cattle and is a vector for severe tick-borne diseases, such as anaplasmosis and babesiosis, and poses a significant threat to the cattle industry. Cattle ticks show increasing acaricide resistance, which creates an additional problem concerning the inefficient che...
Background
Hormone receptors exert their function through binding with their ligands, which results in cellular signaling activation mediated by genomic or non-genomic mechanisms. The intrinsic molecular communication of tick Rhipicephalus microplus and its host Bos taurus comprises an endocrine regulation involving hormones. In the present study,...
To conduct differential gene expression analysis, ovaries from the cattle tick Rhipicephalus microplus were dissected at three distinct developmental stages (preingurgitated, immature ingurgitated, and mature ingurgitated). Additionally, undissected intact mature males and complete ingurgitated female ticks without ovaries (carcasses) were also col...
Soft ticks pose significant health risks as vectors of various pathogens. This study explored the spatio-temporal distribution and genetic relationships of the soft tick species Argas persicus infesting domestic hens (Gallus gallus domesticus) across different districts in Pakistan. An examination of 778 hens revealed a notable tick infestation pre...
Background: Theileria spp. are responsible for ovine and caprine theileriosis, leading to significant morbidity and mortality in small ruminants. The present study aims to investigate Theileria spp. infections in small ruminants from Southern Punjab in Pakistan, and genetic characterize revealed Theileria spp. isolates.
Methods: A total of 93 sheep...
Ticks and tick-borne diseases have gained increasing attention in recent years due to their impact on public health and significant losses in livestock production. The use of synthetic compounds for tick control is becoming problematic, mainly due to the resistance to commercially available products as well as their toxicity. Therefore, new alterna...
Ticks and tick-borne diseases have gained increasing attention in recent years due to their impact on public health and significant losses in livestock production. The use of synthetic compounds for tick control is becoming problematic, mainly due to the resistance to commercially available products as well as their toxicity. Therefore, new alterna...
Rhipicephalus microplus is a persistent ectoparasite of cattle that causes bovine anaplasmosis and babesiosis, causing economic losses worldwide. Chemical treatment is the primary method for tick control, but the emergence of pesticide-resistant ticks is a major challenge. Alternative biocontrol strategies utilizing entomopathogenic microorganisms...
Ticks pose significant health risks to both wildlife and humans due to their role as vectors for various pathogens. In this study, we investigated tick infestation patterns, tick-associated pathogens, and genetic relationships within the tick species Amblyomma gervaisi, focusing on its prevalence in monitor lizards (Varanus bengalensis) across diff...
In this study, we aimed to develop a comprehensive methodology for identifying amino acid polymorphisms in acetylcholinesterase transcript 2 (AChE2) in acaricide-resistant Rhipicephalus microplus ticks. This included assessing AChE2 expression levels through qPCR and conducting 3D modeling to evaluate the interaction between acaricides and AChE2 us...
The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we prop...
The expression of the Fasciola hepatica carboxylesterase type B (CestB) gene is known to be induced upon exposure to the anthelmintic triclabendazole (TCBZ), leading to a substantial rise in enzyme-specific activity. Furthermore, the nucleotide sequence of the CestB gene displays variations that can potentially result in radical amino acid substitu...
Sarcoptes scabiei is an ectoparasite of humans and animals that causes scabies. The Rhipicephalus (Boophilus) microplus is a blood-sucking ectoparasite that transmits various pathogens. These two parasites have caused great losses to a country’s dairy and agriculture sectors. The aim of this study was to determine the in vitro and in silico efficac...
Anaplasma (A.) ovis is the most important cause of anaplasmosis in small ruminants. The current study was planned to estimate the molecular prevalence, risk factors, and phylogenetic analysis of A. ovis infection in sheep and goats from different agro-climatic regions of Central and Southern Punjab, Pakistan. A total of 400 jugular blood samples we...
The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we prop...
The expression of the Fasciola hepatica Carboxylesterase type B (CestB) gene is induced in response to the anthelmintic triclabendazole (TCBZ), resulting in a significant increase in enzymatic specific activity. Furthermore, the amino acid sequence of CestB exhibits variations that could lead to substantial amino acid substitutions at the ligand-bi...
Aspergillus flavus has been found to be an effective entomopathogenic fungus for various arthropods, including ticks. In particular, natural fungal infections in cattle ticks show promise for biocontrol of the Rhipicephalus (Boophilus) microplus tick, which is a major ectoparasite affecting cattle worldwide. Our study aimed to elucidate the specifi...
Simple Summary
Rhipicephalus (Boophilus) microplus, commonly known as the cattle tick, is an ectoparasite that affects all terrestrial vertebrates, including livestock. As animal husbandry is the backbone of Pakistan’s economy, tick infestation results in significant economic losses for farmers annually. Given the reported resistance of various tic...
As the most important bovine ectoparasite, the southern cattle tick Rhipicephalus microplus transmits lethal cattle diseases such as babesiosis and anaplasmosis, costing the global livestock industry billions of dollars annually. To control cattle ticks, preventive treatment of cattle with pesticides is a common practice; however, after decades of...
The present study was conducted for epidemiological examination of seasonal tick infesting on livestock animals (cattle and buffalo). The investigation was carried out from March 2019 to February 2020 period at different districts of western regions of Uttar
Pradesh. The western regions comprise of Amroha (AMR), Moradabad (MBD), Rampur (RMP), Bare...
Rhipicephalus microplus is a major bovine ectoparasite that negatively impacts the cattle industry. The acaricidal activity of Datura innoxia ethanolic plant extract against R. microplus, compared with trichlorfon, was examined using the adult immersion test (AIT), and larval packet test (LPT). In vitro acaricidal activity of the selected plant ext...
Introducción: Las moscas sierra (Hym. Diprionidae) afectan las coníferas y otras especies forestales en el hemisferio norte, reduciendo la productividad del bosque y causando la muerte de los rodales, lo que justifica acciones de control.Objetivo: Obtener cepas baculovíricas de poblaciones de moscas sierra mexicanas y explorar su potencial para ela...
Fasciola hepatica anthelmintic resistance may be associated with the catalytic activity of xenobiotic metabolizing enzymes. The gene expression of one of these enzymes, identified as carboxylesterase B (CestB), was previously described as inducible in adult parasites under anthelmintic treatment and exhibited a single nucleotide polymorphism at pos...
Las garrapatas se encuentran en Mxico y son un vector biolgico de enfermedades hemoparasitarias del ganado bovino. Se recolectaron garrapatas Dermacentor albipictus (Packard, 1869) en equinos, de Tapalpa, Jalisco, una zona de clima templado con presencia de Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida, Ixodidae) y reportes de ana...
Phytochemicals are directly involved in therapeutic treatment or precursors to synthesize useful drugs. The current study was aimed to evaluate the phytocompounds and their biopotentials using methanolic and n-hexane extracts of various parts of Oxalis pes-caprae. For the phytochemical analysis, standard procedures were used, whereas Aluminum Chlor...
Livestock industry is an essential part of Pakistan’s economy, and a variety of ruminants (including sheep and goats) are reared for the increasing demand of milk, meat and hide products. Haemoparasitic illnesses such as theileriosis, anaplasmosis, and babesiosis are a significant health risk for small ruminants in our country. Information regardin...
Despite advancement in modern medicines, plant derived medicines have still wide range utilities as they have less side effects and are cheap and biocompitable. Sassurea lappa is an extensively used plant in traditional medicinal formulations. Plant roots are used to cure various diseases including cancer, rheumatic pain, abdominal and nervous diso...
En México, los bosques de pino son afectados por plagas insectiles, dentro de las cuales destacan las avispas de la familia Diprionidae, conocidas como moscas sierra ―insectos causantes de defoliaciones en grandes extensiones de pinos y juniperus a lo largo del territorio nacional―, por lo que existe la necesidad de implementar medidas de control....
Ticks are hematophagous ectoparasites that infest a diverse number of vertebrate hosts. The tick immunobiology plays a significant role in establishing and transmitting many pathogens to their hosts. To control tick infestations, the acaricide application is a commonly used method with severe environmental consequences and the selection of tick-res...
Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B en...
We describe the selection, culture, maintenance and rearing of the cattle tick Rhipicephalus (Boophilus) microplus at CENAPA-SENASICA-SADER, Mexico, where reference susceptible and acaricide-resistant tick strains are reared and toxicologically tested. The purpose of maintaining these tick strains is to evaluate the efficacy of diverse acaricides p...
Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe liver disease clinically known as fasciolasis in a large number of wild animals, several livestock species as well as humans, prevention and control of fasciolasis is made by massive use of anthelmintic compounds on livestock and inevitably this practic...
This study was conducted in four districts (Malakand, Swat, Bajaur and Shangla) of Northern Pakistan to investigate the prevalence, associated risk factors and phylogenetic analyses of Theileria and Anaplasma species in small ruminants. A total of 800 blood samples, 200 from each district, were collected from apparently healthy animals. PCR assays...
Ascariasis is a common public health problem of preschool and primary school children in developing countries like Pakistan. The aim of the present study was to determine the prevalence and pattern of Ascaris lumbericoides (A. lumbricoides) infection among children residing in urban areas of Lahore, to provide information on ascariasis to promote a...
The ability of ethyl-4-bromophenylcarbamate (LQM 919) and ethyl-4-chlorophenylcarbamate (LQM 996) to induce in vivo apoptosis of Rhipicephalus microplus ovarian cells and in vitro apoptosis of tick and mammalian cell culture was evaluated. The ovaries of engorged females treated with 1 mg mL−1 LQM 919 or LQM 996 presented more (p < 0.001) peroxidas...
Background:
Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. The physiological effects and phenotypes of the mutations that cause acaricide resistance are not always well understood or characterized. Single nucleotide polymorphisms (SNPs) that confer cypermethrin knockdown resistance (kdr) have...
Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. SNPs in the para-sodium channel gene that confer c...
Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus . Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. SNPs in the para-sodium channel gene that confer...
Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. Single Nucleotide Polymorphisms (SNPs) in the para...
The purpose of this work was to contribute to the understanding of the mechanism of action of two new ixodicides. The histological and ultrastructural alterations of Rhipicephalus microplus oocytes (San Alfonso strain) treated with two new ethyl-carbamates (ethyl-4-bromophenyl carbamate and ethyl-4-chlorophenyl carbamate) by the adult immersion tes...
RESUMEN. El artrópodo Rhipicephalus microplus, es un ectoparásito hematófago que representa un serio problema en la industria ganadera mundial, por su gran capacidad expoliativa y vectorial para transmitir organismos patógenos al ganado bovino, como Babesia spp. y Anaplasma spp., En este sentido, R. microplus, posee una amplia variedad de mecanismo...
La garrapata R. microplus, es la especie de mayor incidencia económica y sanitaria debido a su capacidad expoliativa y vectorial de transmisión de agentes infecciosos como Babesia bigemina, Babesia bovis y Anaplasma marginale. En este sentido, R. microplus, posee una amplia variedad de mecanismos inmunológicos de protección, incluyendo la producció...
Anaplasma marginale is an intraerythrocytic bacterium transmitted by ticks that causes bovine anaplasmosis. One of the limitations in the study of this microorganism has been the difficulty to cultivate it in vitro. To date, the cultivation of Mexican strains of A. marginale has not been reported. In this work we show the first approach to establis...
Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcu...
Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcu...
Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcu...
Publicaly available genomes used for this study
The 265 publicaly available genomes, from 46 species, were included, 40 of them are type strains (marked in bold). Genome statics were taken from NCBI. The third column gives the strain code used for figures 1, 2 and 4.The red bold row shows Staphylococcus saprophyticus UBA1057, which was removed from...
Functional enrichment analysis
GO enrichment analysis via Blast2GO PRO.
Newly sequenced genomes for this study
Genome assembly statistics of thenewly sequenced Staphylococci strains.
List of the 177 SGF without signals of recombination
Complete list of the 177 SGF without signals of recombination, which are our orthologous groups. Protein accession numbers were taken from the S. aureus reference genome TW20, the corresponding gene name was checked in Uniprot.
Robinson-Foulds distances between all the SGF trees
Histogram of the Robinson-Foulds distances estimated between all the SGF trees. There were no two identical topologies and on average topologies are 47% similar to each other.
Intra-species genetic diversity for S. aureus and S. epidermidis
Histograms of the intra-species nucleotide diversity for S. aureus and S. epidermidis.
Neighbour Nets and recombination test for rpoB and tuf
Phylogenetic networks built with SplitsTree, for two (rpoB and tuf) of the usual taxonomic markers. These markers are part of the 208 single copy genes but failed the recombination test. Strain labels are not shown for sake of clarity.
Type IV secretion system is composed by a group of proteins associated with substrate transfer between bacteria and eukaryotic organisms. VirB11 is a protein of this system and in A. marginale it induces partial protection in cattle immunized with external membrane fragments of the rickettsia. The objectives of this study were: to determine the con...
The goal of the present study was to assess the gene expression of xenobiotic metabolizing enzymes (XMEs) Cytochrome P-450 (CYP) and carboxylesterase (CE) related to detoxification of synthetic pyrethroids, plus acetylcholinesterase (AChE), in field isolates of acaricide-resistant Rhipicephalus microplus. The XMEs expression levels were assessed by...
The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resi...
Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 c...
A zymogram assay for Glutathion S Transferase (GST) was assessed, as an alternative diagnostics procedure for acaricide resistance detection in the cattle tick Rhipicephalus microplus using tick extracts from different developmental stages. GST is involved in the metabolic neutralization of toxins in a broad range of arthropods and associated with...
RESUMEN. Se colectaron garrapatas de Rhipicephalus (Boophilus) microplus durante los años 2010 a 2015 de bovinos infestados experimentalmente. Estas garrapatas mostraron signos de infección natural, como la presencia de exudado en el hipostoma y poro genital, cambio de coloración de la cutícula y bloqueo en la oviposición de hembras ingurgitadas qu...
RESUMEN. Como un procedimiento alternativo al diagnóstico de la resistencia de Rhipicephalus microplus a los acaricidas, este trabajo hace la evaluación de un ensayo de zimogramas de la enzima glutatión S transferasa (GST) a partir de extractos obtenidos de diferentes estadios de desarrollo de la garrapata del ganado. Para este ensayo, se determina...
The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previous works demonstrated that the pharmacological perturbation of this system inhibits oviposition in the cattle tick Rhipicephalus microplus. In this work, we describe a physiologically active whole-mount preparation of the contractile tick ovary that al...
Paenibacillus larvae strain MEX14 is a facultative anaerobic endospore-forming bacterium that infects Apis mellifera larvae. Strain MEX14 was isolated from domestic bee larvae collected in a backyard in Mexico City. The estimated genome size
was determined to be 4.18 Mb, and it harbors 4,806 protein coding genes (CDSs).
Rhipicephalus (Boophilus) microplus females do not deposit eggs during symptoms of genital infection. In experimental conditions 5% of engorged female ticks showed symptoms of bacterial infection. The symptoms are evident females secreted a distinctive yellow exudate through the genital orifice and eventually died. We obtained 62 bacterial isolates...
The objective of this work was to study and characterize the Rhipicephalus (Boophilus) microplus embryogenesis from freshly laid eggs to hatching larvae. Eggs were fixated every 24 hr. and treated eggs were placed on a microscope glass slide and stained with 4',6-diamidino-2-phenylindole (DAPI).Specimens were imaged on an epifluorescence microscope...
The molecular target of the acaricide amitraz is considered to be the octopamine receptor (OAR). Changes in the OAR sequence gene could be responsible of the cattle ticks becoming resistant to amitraz. A putative octopamine receptor was cloned and sequenced from a susceptible and an amitraz resistant Mexican reference strains. Two alleles were foun...
Steroid hormone-like molecules exert control on developmental changes of helminthes into fully reproductive adults. All invertebrates exhibit a very delicate balance of circulating steroids and the ubiquitous enzymes carboxylesterases are necessary for hydrolyzing excess hormone levels or completely depleting it for the final transition into adulth...
The cattle tick Rhipicephalus microplus, is one of the most damaging livestock ectoparasites. Tropical tick infestation limits the introduction of high-yield bovine varieties because they do not have immunity to the diseases transmitted by these ectoparasites. This tick is usually controlled with chemical acaricides but their indiscriminate use has...
In the present work, we evaluate in vivo the activity of carboxylesterase of Fasciola hepatica exposed to triclabendazole. We observed a statistically significant increase in enzyme activity at 24 and 48h post treatment (P<0.01 and P<0.001, respectively). The zymogram of cytosolic fractions identified a protein of 170kDa containing the carboxyleste...
The cattle tick Rhipicephalus microplus, is one of the most damaging livestock ectoparasites. Tropical tick infestation limits the introduction of high-yield bovine varieties because they do not have immunity to the diseases transmitted by these ectoparasites. This tick is usually controlled with chemical acaricides but their indiscriminate use has...
The purpose of this study was to describe an unreported entomopathogenic fungus that naturally infects the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Engorged female ticks, showed symptoms of fungal infection after controlled tick infestation of cattle. Infected ticks developed a distinctive dark colour, a pale mould grew ov...
The purpose of this book is to convey the available knowledge on cell cultures based on the expertise of the authors themselves, to those Spanish speaking persons that are interested in this subject. This book contains information on basic data, metrical units, proper nomenclature and appropriate definitions to simplify its understanding. Similarly...
Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida: Ixodidae) were infested by small Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) flies. Larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle throug...
During experimental Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) infestation on cattle, approximately 5% of engorged female ticks showed symptoms of bacterial infection. The affected ticks were unable to oviposit, secreted a distinctive yellow exudate through the genital orifice and eventually died. Microscopic analysis of tick exudate sho...
Abstract: An acaricide-resistant and an acaricide-susceptible strain of Rhipicephalus microplus ticks, were evaluated during a 40 day larval development period for carboxylesterase expression on SDS-PAGE, with the objective of establishing the optimal larval age for carboxylesterase specific activity measurement. Experimental results demonstrated t...
The present study reports the cloning and expression of a Caenorhabditis elegans Cathepsin B-like Protease (CBP), with the objective of obtaining a recombinant enzyme bearing biochemical properties similar to natural CBP reported in the literature for C. elegans and parasitic nematodes. The gene was isolated by PCR from C. elegans cDNA, resulting a...
Primary cell cultures from different tick organs are a valuable tool for host parasite research in the study of the protozoan Babesia sp., which infects different organs of the tick. In this work we describe the generation of midgut, salivary gland, and ovary primary cell cultures from dissections of Boophilus microplus. Midguts, salivary glands, a...
A Haemonchus contortus recombinant Cysteine Protease (CP) was expressed in the baculovirus system. The CP gene was isolated by PCR from H. contortus cDNA, the PCR amplicon was cloned downstream to the polihedrin promoter within a bacterial expression vector, Sf9 insect cells were used for simultaneous co-transfection with the CP-vector and baculovi...
A Haemonchus contortus recombinant Cysteine Protease (CP) was expressed in the baculovirus system. The CP gene was isolated by PCR from H. contortus cDNA, the PCR amplicon was cloned downstream to the polihedrin promoter within a bacterial expression vector, Sf9 insect cells were used for simultaneous co-transfection with the CP-vector and baculovi...
Abstract: This study was done with the objective of establishing a relationship between cytochrome P-450 gene expression and acaricide resistance levels in Mexican isolates of R. microplus, for that purpose the cytochrome P-450 expression levels were measured by real time PCR quantification on acaricide sensitive and resistant strains of ticks, as...
This study was made to assess SDS-PAGE monooxygenases zymograms in the presence of synthetic monooxygenase enzyme substrates, in order to establish a reliable methodology to measure monooxygenase activity levels on acaricide resistant ticks and finding a probable association between monooxygenase enzymatic levels and B. microplus acaricide resistan...
Anthelmintic resistance detection of ruminant gastroenteric parasitic nematode Heamonchus contortus, can be achieved by molecular probes and DNA amplification assays, this advanced methodologies are faster and cheaper to apply than conventional bioassays, however for better accuracy of a DNA-based test, it is necessary first to clarify the details...
Rhipicephalus microplus in vitro cell culture have been useful in the study of acaricide resistance in ticks, a methodology for heterologous gene transference into these cultures may help to study several aspect of the cell biology and gene expression associated with acaricide resistance. In this study we assessed a pantropic retroviral gene transf...
We report a molecular cloning and expression in the bacu- lovirus system of an Haemonchus contortus recombinant cystein protease (CP) gene, with the objective of obtaining a recombinant CP with biochemical and immunochemical properties similar to the natural one obtained from the parasite, with applications to the diagnosis prevention and control o...
La identificación de resistencia a los antihelmínticos del nematodo parásito Haemonchus contortus, se hace mediante un complicado bioensayo que requiere larvas de nematodos de referencia, así como de campo, cultivadas en laboratorio y expuestas a diferentes productos. Con el fin de evaluar una posible alternativa diagnóstica, se hicieron ensayos en...
Phospholipid-hydroperoxide glutathione peroxidase (PHGPx) enzymes are associated with cellular protection by the role they play in reducing hydroperoxides of phospholipids, thereby preventing membrane lipoperoxidation. As part of their toxic effect, some pesticides stimulate peroxidation of cellular membranes. We isolated and sequenced a PHGPx gene...
