Raphaela Fritsche

Raphaela Fritsche
Charité Universitätsmedizin Berlin | Charité · Berlin Institute of Health - Metabolomics Platform

Dr.

About

51
Publications
5,747
Reads
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643
Citations
Introduction
Experienced experimental scientist in the field of metabolomics, proteomics and molecular biology. Extensive record in the field of systems biology and translational medicine. Highly experienced in standard laboratory skills and molecular analysis techniques. Familiar with experimental design, data processing, statistical analysis and writing of scientific reports.
Additional affiliations
January 2019 - present
Max-Delbrück-Centrum für Molekulare Medizin and Berlin Institute of Health
Position
  • PhD Student
Description
  • The BIH Metabolomics Platform pursues mass spectrometry metabolomics research with clear translational medicine potential. Own research topic: Metabolic alterations in osteosarcoma.
January 2018 - December 2018
Max Delbrück Center (BIH Metabolomics Platform + MDC Molecular Cardiovascular Research, Willnow Lab)
Position
  • PhD Student
Description
  • Main project: Alzheimer`s disease is the most common form of dementia and one of the greatest challenges of the 21st century. To unravel the complex pathology of Alzheimer`s disease we will use MS-based metabolomics techniques.
July 2016 - December 2017
Max-Delbrück-Centrum für Molekulare Medizin and Berlin Institute of Health
Position
  • PhD Student
Description
  • Main project: To gain new insights into neuroblastoma (NB) pathogenesis we will analyze NB cell lines and tissue samples using MS-based -omics techniques. While NB tumors show dependency on glycolysis this may be a promising therapeutic strategy.
Education
September 2003 - February 2008
Charité Universitätsmedizin Berlin, Pathology
Field of study
  • Molecular and cell biology
April 1999 - April 2003
Freie Universität Berlin
Field of study
  • Human Biology/Zoology

Publications

Publications (51)
Article
The activation of oncogenes via genomic amplification is a hallmark of the cancer genomes, yet such amplifications frequently also encompass neighboring genes. Such passenger genes are involved in essential cellular activities. We hypothesized that co-amplification of passenger genes create cancer-specific collateral therapeutic vulnerabilities. To...
Article
Full-text available
The identification of endogenous metabolites has great potential for understanding the underlying tissue processes occurring in either a homeostatic or a diseased state. The application of gas chromatography-mass spectrometry (GC-MS)-based metabolomics on musculoskeletal tissue samples has gained traction. However, limited comparison studies exist...
Article
Full-text available
The AbsoluteIDQ p400 HR kit is a commercial product for targeted metabolomics. While the kit has been validated for human plasma and serum, adherent cell lysates have not yet been evaluated. We have optimized the detection of polar and lipid metabolites in cell lysates using the kit to enable robust and repeatable analysis of the detected metabolit...
Article
Recently, there has been growing interest in short-chain fatty acids (SCFA) and ketone bodies (KB) due to their potential use as biomarkers of health and disease. For instance, these diet-related metabolites can be used to monitor and reduce the risk of immune response, diabetes, or cardiovascular diseases. Given the interest in these metabolites,...
Article
Full-text available
Using manual derivatization in gas chromatography-mass spectrometry samples have varying equilibration times before analysis which increases technical variability and limits the number of potential samples analyzed. By contrast, automated derivatization methods can derivatize and inject each sample in an identical manner. We present a fully automat...
Preprint
Full-text available
Aims Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization o...
Article
Full-text available
Purpose: Nesfatin-1 is produced in various tissues of the body including the hypothalamus. Neuroprotective properties of the neuropeptide hormone Nesfatin-1 were recently described. The aim of the study was to analyze the molecule Nesfatin-1 as a possible biomarker in POAG with neuroprotective properties pointing out the retinal-hypothalamic axis...
Article
Full-text available
Osteosarcoma (OS) is a primary malignant bone tumor and OS metastases are mostly found in the lung. The limited understanding of the biology of metastatic processes in OS limits the ability for effective treatment. Alterations to the metabolome and its transformation during metastasis aids the understanding of the mechanism and provides information...
Conference Paper
Full-text available
High visceral adiposity (VA) and low skeletal muscle area (SMA) are poor prognostic factors in many cancers. In this study we aimed to characterise the perioperative human metabolome (combined end-product of cellular metabolism) in differential body compositions and correlate preferential metabolic pathways to five-year cancer outcomes.
Article
Full-text available
A gas chromatography mass spectrometry (GC-MS) metabolomics protocol was modified for quenching, harvesting, and extraction of metabolites from adherent cells grown under high (20%) fetal calf serum conditions. The reproducibility of using either 50% or 80% methanol for quenching of cells was compared for sample harvest. To investigate the efficien...
Article
Background and aims: Gonadal hormones are mainly thought to account for sex and gender differences in the incidence, clinical manifestation and therapy of many cardiovascular diseases. However, intrinsic sex differences at the cellular level are mostly overlooked. Here, we assessed sex-specific metabolic and functional differences between male and...
Article
Background Personalised Medicine is one of the hallmarks of future medicine. Sex and gender differences exist in the incidence, clinical manifestation and outcome of cardiovascular diseases. Gonadal hormones are thought to account for most of these sex differences. However, besides hormones, sexual dimorphisms at the cellular level may also contrib...
Data
Table S3. Results of the Mass Spectrometry Analysis of Cell Surface Proteomes of WT and S2KO Neurons, Related to Figure 4 Excel file containing the complete results of the quantitative mass spectrometry analysis of protein composition of cell surface fractions derived from WT and S2KO primary neurons.
Article
VPS10P domain receptors emerge as central regulators of intracellular protein sorting in neurons with relevance for various brain pathologies. Here, we identified a role for the family member SorCS2 in protection of neurons from oxidative stress and epilepsy-induced cell death. We show that SorCS2 acts as sorting receptor that sustains cell surface...
Article
Full-text available
Metabolic reprogramming is as a hallmark of cancer, and several studies have reported that BRAF and KRAS tumors may be accompanied by a deregulation of cellular metabolism. We investigated how BRAFV600E and KRASG12V affect cell metabolism, stress resistance and signaling in colorectal carcinoma cells driven by these mutations. KRASG12V expressing c...
Article
Full-text available
The RAF-MEK-ERK signalling pathway controls fundamental, often opposing cellular processes such as proliferation and apoptosis. Signal duration has been identified to play a decisive role in these cell fate decisions. However, it remains unclear how the different early and late responding gene expression modules can discriminate short and long sign...
Article
Full-text available
The RAF‐MEK‐ERK signalling pathway controls fundamental, often opposing cellular processes such as proliferation and apoptosis. Signal duration has been identified to play a decisive role in these cell fate decisions. However, it remains unclear how the different early and late responding gene expression modules can discriminate short and long sign...
Article
Full-text available
Targeted therapies within the RAS/RAF/MEK/ERK signalling axis become increasingly popular, yet cross-talk and feedbacks in the signalling network lead to unexpected effects. Here we look systematically into how inhibiting RAF and MEK with clinically relevant inhibitors result in changes in PI3K/AKT activation. We measure the signalling response usi...
Article
Full-text available
Impedance-based technologies are advancing methods for measuring proliferation of adherent cell cultures non-invasively and in real time. The analysis of the resulting data has so far been hampered by inappropriate computational methods and the lack of systematic data to evaluate the characteristics of the assay. We used a commercially available sy...
Article
Full-text available
The epidermal growth factor receptor (EGFR) signaling network is activated in most solid tumors, and small-molecule drugs targeting this network are increasingly available. However, often only specific combinations of inhibitors are effective. Therefore, the prediction of potent combinatorial treatments is a major challenge in targeted cancer thera...
Article
Full-text available
Protein levels within signal transduction pathways vary strongly from cell to cell. For example, it has been reported that concentrations of the last kinase within the MAPK signalling module, Erk, varies about four-fold between clonal cells under the same conditions. In the present study, we analysed how signalling pathways can still process inform...
Article
Full-text available
In osteosarcoma patients the development of metastases, often to the lungs, is the most frequent cause of death. The aim of this study was to elucidate the molecular mechanisms governing osteosarcoma development and dissemination and, thereby, to identify possible novel drug targets for improved treatment. Osteosarcoma samples were characterized us...
Article
Full-text available
CD 52 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that is expressed abundantly on all lymphocytes, monocytes, macrophages, eosinophils and in the male genital tract. To date, the physiological role of CD52 on lymphocytes has not been elucidated. However, an antibody directed to CD52 called CAMPATH-1H has been shown to be capable o...
Article
Full-text available
Due to morphological similarities of high-grade synovitis in rheumatoid Arthritis (RA) and mesenchymal, semimalignant tumors and the hypothesis that RA progression is not only inflammation-related, but also determined by tumor-like mechanisms, a comparison was made between expression profiles of RA, giant cell tumor of bone (GCT) and normal synoviu...
Thesis
Knochentumore stellen mit nur 0,2% aller menschlichen Tumore sehr seltene primäre Neoplasien des skelettalen Systems dar. Diese Dissertation beschreibt die genomweite Microarray Analyse von Osteosarkomen und Riesenzelltumoren. Mit einer Microarray Analyse von konventionellen und metastatischen Osteosarkom-Geweben verglichen zu einer Osteoblasten-Pr...
Article
Full-text available
Inflammatory rheumatic diseases with their unclear aetiology are a challenge for the routine clinical practice. The dominating inflammatory processes with many facets of autoimmune phenomena have been extensively studied during the last decades. Modern high throughput technologies provide for the first time the opportunity to obtain an insight into...
Article
Full-text available
Inflammatory rheumatic diseases with their unclear aetiology are a challenge for the routine clinical practice. The dominating inflammatory processes with many facets of autoimmune phenomena have been extensively studied during the last decades. Modern high throughput technologies provide for the first time the opportunity to obtain an insight into...
Article
H-REV107-1, a known member of the class II tumor suppressor gene family, is involved in the regulation of differentiation and survival. We analyzed H-REV107-1 in non-small cell lung carcinomas, in normal lung, and in immortalized and tumor-derived cell lines. Sixty-eight percent of lung tumors revealed positive H-REV107-1-specific staining. Further...
Article
Full-text available
Almost no data exist concerning the role of antibody-mediated mechanisms in human acute cellular liver allograft rejection (ACR). Therefore, the aim of this study was to determine whether ACR is associated with depositions of complement split products and increased infiltration by B-lymphocytes, plasma cells and macrophages. A total of 35 liver bio...
Article
Full-text available
Giant cell tumors (GCTs) of the bone are osteolytic neoplasms with variable degrees of aggressiveness. The aim of this study was the molecular characterization of GCT tissue. We established gene expression profiles and discovered a number of genes that have not been described in GCTs before. RNA was prepared from 7 cryopreserved GCTs (primary tumor...
Article
Full-text available
The number of endoprosthesis revision operations is increasing. In a previous analysis using cDNA microarrays, differentially expressed genes were detected in the wear-particle-induced membrane and in the infectious periprosthetic membrane. This study aims at validation of these gene expression profiles in order to find genes that are potentially r...
Article
Full-text available
A previous cDNA-microarray analysis described constantly differentially expressed genes in wear particle induced and infectious SLIM (synovial-like interface membrane). This study aims to validate the cDNA microarray data in order to approve differences of the gene expression profiles of RNA and proteins. Tissue from 16 wear particle induced and 20...
Article
Full-text available
Background. A previous cDNA-microarray analysis described constantly differentially expressed genes in wear particle induced and infectious SLIM (synovial-like interface membrane). This study aims to validate the cDNA microarray data in order to approve differences of the gene expression profiles of RNA and proteins. Methods. Tissue from 16 wear pa...

Questions

Questions (2)
Question
The target should not be activated upon Erk pathway, so only via PI3K/Akt pathway.
Question
I would like to test an inhibitor but I don`t have these cells and I will not buy them for only one test. So is anyone able to send me a cryo stock or a cultured flask?

Projects

Projects (4)
Project
Ostesoarcoma (OS) is a primary malignant bone tumor that usually develops in children and young adults during periods of rapid growth.OS is the most common radiation-induced sarcoma. OS metastases are mostly found in the lung (85-90%) and are still the primary cause of death in OS patients. Nearly all patients develop lung metastases which are chemo-resistant. Current treatment options for OS involves surgery combined with chemotherapy, but unfortunately, the treatment of OS patients has remained unchanged since the 1970s. The current study intent to identify key metabolic signatures to elucidate how such metabolic changes are advantageous to the survival of malignant OS cells which may help to explain therapy resistance.
Project
Alzheimer`s disease is the most common form of dementia and one of the greatest challenges of the 21st century. It is a chronic neurodegenerative disease that normally starts slowly and become worsens over time. Despite intensive research, current treatment options cannot stop Alzheimer's from progressing; they only can temporarily slow the worsening of dementia symptoms and improve quality of life. Therefore, an attempt is necessary to find better ways for delay its onset, treatment and prevent patients from developing of Alzheimer`s disease. Over the last years, metabolomics was used for several entities to monitor molecular alterations associated with disease development and progression. Metabolomics is a powerful tool to discover novel biomarkers, discover new therapeutic targets and identify molecules for metabolic perturbation. To unravel the complex pathology of Alzheimer`s disease we will analyze transgenic mouse strains using MS-based metabolomics techniques.