Ramses Belda Perez

• PhD Student at University of Teramo

MicroRNAs (miRNAs) have acquired an increased recognition to unravel the complex molecular mechanisms underlying Diminished Ovarian Reserve (DOR), one of the main responsible for infertility. To investigate the impact of miRNA profiles in granulosa cells and follicular fluid, crucial players in follicle development, this study employed a computatio...

Despite significant advances, assisted reproductive techniques (ARTs) remain suboptimal due to challenges linked to embryo development and quality, often attributed to the epigenetic reprogramming process and environmental factors. The aim of this review is to summarize the function of the oviduct, the organ where fertilization takes place. This or...

The rising incidence of metabolic diseases is linked to elevated blood glucose levels, contributing to conditions such as diabetes and promoting the accumulation of advanced glycation end products (AGEs). AGEs, formed by non-enzymatic reactions between sugars and proteins, build up in tissues and are implicated in various diseases. This article exp...

The oviduct plays a crucial role in the reproductive process, serving as the stage for fertilization and the early stages of embryonic development. When the environment of this organ has been mimicked, it has been shown to enhance in vitro embryo epigenetic reprogramming and to improve the yield of the system. This study explores the anatomical int...

Nowadays there is an increasing demand for assisted reproductive technologies due to the growth of infertility problems. Naturally, fertilization occurs in the oviduct, where the oviductal epithelial cells (OECs) secrete many molecules that affect the embryo’s metabolism and protect it from oxidative stress. When the OECs are grown in 3D culture sy...

Nowadays, the adoption of In Vitro Fertilization (IVF) techniques is undergoing an impressive increase. In light of this, one of the most promising strategies is the novel use of non-physiological materials and naturally derived compounds for advanced sperm preparation methods. Here, sperm cells were exposed during capacitation to MoS2/Catechin nan...

Simple Summary Genetically modified pigs are very useful thanks to their applications in basic research, biomedicine, and meat production. There are different methods for producing them, including cloning and the microinjection or electroporation of oocytes and zygotes. Easier techniques are being developed, such as lipofection, which involves the...

The latest IETS report estimated that 1,156,422 in vitro-produced bovine embryos were generated in 2020.These embryos, either from ovum pickup or abattoir-derived oocytes, are the result of the in vitro maturation (IVM), fertilisation, and embryo culture processes. These processes require research and commercial laboratories to either prepare their...

The development of 3D printers and the advances in materials science and tissue engineering in the last years have allowed the implementation of this technology in several research areas. So, it has been used as a tool to create a 3D-oviduct-on-a-chip with poly(dimethylsiloxane) that mimics de physiological environment, which has proved to be usefu...

Study question Are there any potential effects derived from the exposure of spermatozoa to MoS2/catechins nanoflakes during the process of capacitation? Summary answer The spermatozoa exposure to MoS2/catechin nanoflakes do not induce toxicity on spermatozoa and modulate sperm capacitation in a swine in vitro model. What is known already Molybden...

The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on the offspring phenotype are still unknown. We aimed to describe such effects in pigs. Blood samples and growth parameters were collected from piglets derived from in vitro-produced embryos (IVP) with or without...