Ramazan Arıcı

İstanbul University-Cerrahpaşa · Faculty of Veterinary Medicine

BACKGROUND-AIM Transgenesis in livestock allows improvement of desired traits besides being used as animal models for biomedical research. Intracytoplasmic microinjection (ICI) appears to be a favorable alternative for gene delivery to embryos. In this study, effects of different pEGFP-N1 concentrations on fluorescing embryo rate and embryonic deve...

Usage of oocytes obtained from ovaries after long-term cold storage for in vitro embryo production is a promising tool for the protection of wildlife and endangered animal species. Mammalian oocytes are susceptible to oxidative stress with regard to the high lipid content of plasma membranes. Melatonin is known as a powerful antioxidant and anti-ap...

The primary aim of the study was to investigate whether iodixanol and trehalose would have a synergic effect on the cryosurvival of electroejaculated ram semen. Tris‐based diluter was used to prepare 9 different extenders by the addition of iodixanol or trehalose alone or varying combinations of these substances. Diluters were prepared as follows:...

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for...

The potential relationships between Anti-Müllerian Hormone (AMH) concentrations and fertility were investigated by examining pregnancy rates and early pregnancy loss in different age groups of cows. Holstein heifers (17.35 ± 1.35 months, n = 20), young cows (3.25 ± 1.02 years, n = 20), and old cows (6.7 ± 0.80 years, n = 20) were synchronised and t...

Percoll gradient and swim-up are the most common methods used to obtain high quality intact sperm to be used for the in vitro fertilization procedures. In this study, we evaluated the effects of swim-up and percoll gradient methods on DNA integrity and other spermatological parameters. To determine DNA breakdown, toluidine blue staining was used. M...

The motility, sperm membrane and acrosome integrity during post-thawing incubation is an indication of the usability of the semen. The maintenance of higher motility of sperm during post-thawing incubation simultaneously with the maintenance of intact cell membrane and acrosome integrity reflects a greater likelihood of their survival in the female...

This study was conducted to evaluate the protective effects of cholesterol pre-incubation on ovine oocytes exposed to cold stress and vitrification. In the first experiment, cumulus oocyte complexes harvested from ovaries of slaughtered ewes were exposed to 2 mg/ml cholesterol loaded methyl-ßcyclodextrin (CLC) in maturation medium for 2, 4 or 24 h....

Oxidative stress during in vitro culture leads to defects in development of gametes and embryos. Several antioxidants such as cysteamine, L-ascorbic acid, beta mercaptoethanol, cysteine, glutathione, proteins, vitamins have been used to supplement culture media to counter the oxidative stress. This study was conducted to detect the effect of adding...