Rajesh ChaudharyFeinberg School of Medicine Northwestern · Department of Pathology
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February 2007 - June 2008
School of Science, Kathmandu University
- Phyochemical analysis of Cornus capitata plant the bark of which is used in Chinese traditional medicine. We checked its anticancerous property along with other biological properties which can be beneficial for medicinal purpose.
August 2004 - September 2008
Department of Biotechnology, School of Science, Kathmandu University
Field of study
My target protein is 17 kDa, 34 kDa, 54 kDa and I am using the following conditions: Electrophoresis @ 150 V for 55 minutes Transfer @ 100 V for 1 hour in cool condition I am using PVDF membrane 0.2 um pore size. Methanol in transfer buffer is 20%. I use all the products from BioRad excepts my primary and secondary. Primary dilution is 1:1000 in total 5 ml volume and incubated overnight @ 4 degree and 1:10,000 dilution for secondary antibody and incubated at room temperature for 1 hour. My washing steps are after primary incubation is 3 times for 5 minutes each and half an hour for fourth time. And, after 1 hour of incubation with secondary at room temperature, washing was done with 1X TBST for 3 times for 5 minutes each and half an hour for the 4th. time. I have attached herein my blottings and Ponceau staining pictures. Please let me know where I am doing wrong. Thanks in advance. With best regards, Rajesh
How do we interpret higher vs lower stability values in NormFinder? I know that NormFinder returns us with the best combination of genes but with certain stability values. I need to know what does a lower stability value vs higher values indicate.
Any suggestion will be highly appreciated.
Thanks in advance.
I have been looking for a protocol to extract DNA from the leftover of protein extraction. I want to use the leftover of protein extraction (usually, the one left after we transfer supernatant-containing the proteins) -- usually, in the pellet kind of form.
Any suggestions? Looking forward to it. Thanks in advance.
Cheers ! Rajesh
Do any of you have some paper that you can recommend, or maybe some book that has clear and detailed explanations for beta-catenin as well as it role in Wnt-signaling pathways?
Thank you in advance for your kind answers and suggestions.
There is a statement which says something like this: "Although the centrosome has a key role in efficient mitosis in animal cells, it is not essential."
How can something which has got a key role in a process, its absence does't really affect the process?
We know that urea has tubular absorption while creatinine is not. Can we say that its molecular weight might have some kind of affect on whether or not one is reabsorbed while another is not?
If that is true, can you please provide me some evidence in form of paper or some other source?
I would like to know how does coffee affect our whole system - to be more specific, our central nervous system. I mean, how does coffee stimulate our cells and enhance our performance through making us attentive.
I would like to know its mechanisms that might involve the digestive system, HCL secretion and then cell activation.
I am not sure whether it has a direct effect on activation or does it get absorbed into our body, enter into our blood and then have an impact on stimulating cells.
I would appreciate if somebody has some review papers or some materials that can elucidate this mechanism of our whole system - particularly involving the digestive system, central nervous system and the rest of the system accordingly.
Looking forward to your kind suggestion.
I am using Hi-Media Molecular Biology grade water for my work -- particularly, to reconstitute proteinase K which is in powder form. I have stored it for more than a year I guess, at 4 degree centigrade after I opened it. So, do you think I need to re autoclave it before using it? I think, since I have stored it in its already optimal storing condition, I don't need to re autoclave it.
BTW, it has been filtered using 0.2mm filter. So, I fear that if I will autoclave it, the bottle might contain some contaminant or some DNAse or something and it might contain minute particles and I fear it might interfere in the process.
What is your suggestion?