Rajendra Singh

• MVSc, PhD
• Retd Head, Emeritus Professor at Indian Veterinary Research Institute

Aim of study: The aim of this study was to evaluate the effectiveness of a native isolate of Metarhizium anisopliae (Metsch.) Sorokin as a biocontrol agent against fourth instar larvae of the cabbage webworm, Hellula undalis (Fabricius, 1781). Area of study: The research focused on entomopathogenic fungi and particularly examined the impact of diff...

Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for...

Fresh bovine pericardium from the local abattoir was cut into 2 cm x 2 cm size pieces and crosslinked with 1% solution of glutaraldehyde (GA), formaldehyde (FA), polyethylene glycol (PEG), hexamethylene di-isocyanate (HMDI) and 1-ethly-3-(3-dimethylaminoprophl)-carbodiimide (EDC). Other pieces were made acellular and then crosslinked with above che...

Green lacewing, Chrysoperla zastrowi (Esben-Petersons) (Neuroptera: Chrysopidae) is the most effective polyphagous predator of different species of aphids and is commonly known as “aphid lion.” During the study, the green lacewing adults were collected from the flowers of different crop plants in Meerut region. The experiment on feeding potential o...

A field experiment was conducted for two years (2021-22 and 2022-23) at Crop Research Center, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, to ascertain the influence of various meteorological parameters on population fluctuations of Helicoverpa armigera on tomato. The peak period of occurrence o...

Objective The purpose of the study was to compare the healing potential of bubaline small intestinal matrix (bSIM) and fish swim bladder matrix (FSBM) on full-thickness skin wounds in rabbits. Method Four full-thickness skin wounds (each 20×20mm) were created on the dorsum of 18 rabbits that were divided into three groups based on treatment: untre...

Chrysoperla zastrowi (Esben-Petersons) is known as golden eyes and aphid lions, is a cosmopolitan polyphagous and efficient predator commonly found in a wide range of agricultural habitat. It is an example of one of these species that is not predacious in the adult stage. Adults feed only on pollen, nectar and aphid honeydew. Larval stage of green...

Aims We aimed to investigate the prevalence, pathology, and characterization of S. dysgalactiae subsp. equisimilis (SDSE) in slaughtered pigs of India. Methods and results We collected 1254 morbid tissues (lungs-627, spleen-627) and 627 heart-blood from 627 slaughtered pigs. The bacterial isolation, antibiogram, virulence gene profiling, and mouse...

Helicoverpa armigera (Hubner), stands out as a serious agricultural menace, inflicting substantial damage to a wide range of crops, including cotton, corn, tomato, and soybean. One promising approach involves the use of entomopathogenic fungi, which are naturally occurring pathogens that infect and kill insects. Aim of this study, high-throughput s...

Fibrinous pericarditis, predominantly of bacterial origin, is frequently documented in slaughtered pigs causing great economic loss to the pig production. The present report describes the rare cases of Mycoplasma hyopneumoniae induced fibrinous pericarditis in post-weaned crossbred piglets (n=7). The piglets showed the lesions of fibrinous pericard...

Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV lo...

Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV lo...

The chickpea pod borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a destructive pest of chickpeas that is difficult to control using conventional methods. We isolated and evaluated the virulence of five isolates of Metarhizium anisopliae against larvae of H. armigera. All isolates of M. anisopliae, SVPUAT 1, SVPUAT 2, SVPUAT 3, SVPUA...

Pasteurella multocida is widely distributed in all pig-rearing countries, affecting the economic viability and profitability of pig production. The present research highlights the molecular characterization and pathology of untypeable capsular serotypes of P. multocida in slaughtered pigs from prominent pig-rearing states of India. The prevalence o...

The Foot-and-Mouth disease is highly contagious acute viral disease of livestock inflicting huge economic loss to the farmers. The limited knowledge regarding the pathological lesions vis-a-vis distribution of the FMDV in lesser explored endocrine glands and important vital organs other than the target organs of infected calves prompted us to take...

Porcine Respiratory Disease Complex (PRDC) is an unequivocally leading cause of economic losses to the pig industry. To investigate the pathogens associated with PRDC, a total of 900 lungs with gross lesions and 125 lungs with no appreciable gross lesions were collected from the abattoirs and subjected to pathological investigation for distribution...

Purpose Mesenchymal stem cell (MSC) transplantation has been touted as a possible treatment for spinal cord injury (SCI) patients’ locomotor and neurological rehabilitation. The intralesional injection of MSC is a typical delivery route for SCI, although this can result in further injury. We conducted the study with the aim of characterizing canine...

Aflatoxins, particularly AFB1, are the most common feed contaminants worldwide, causing significant economic losses to the livestock sector. The current paper describes an outbreak of aflatoxicosis in a herd of 160 male young goat kids (3–4 months), of which 68 young kids succumbed over a period of 25 days after showing neurological signs of abnorm...

Muscle development is an important priority of pig breeding programs. There is a considerable variation in muscularity between the breeds, but the regulation mechanisms of genes underlying myogenesis are still unclear. Transcriptome data from two breeds of pigs with divergent muscularity (Mali and Hampshire) were integrated with histology, immunofl...

Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for...

Aflatoxins, particularly Aflatoxin B1 (AFB1), are the most common feed contaminants worldwide, causing significant economic losses to the livestock sector. The current paper describes an outbreak of aflatoxicosis in a herd of 160 male young goat kids (3–4 months), of which 68 young kids succumbed over a period of 25 days after showing neurological...

As a biological control agent, entomopathogenic fungus (EPF) can give an alternative to high-risk pesticides. Metarhizium anisopliae is one of the most promising pest controls EPF in the worldwide. On fungi-specific selective media, the EPF isolate SVPUAT was isolated from Western Uttar Pradesh, India. The isolate was identified as M. anisopliae (G...

In the present study, we reported the distribution of diff erent serotypes of Streptococcus suis from the diseased slaughtered pigs of India. Of 664 morbid tissues of slaughtered pigs, 41 were confi rmed to be S. suis by PCR targeting the gdh gene. Majority of the isolates belonged to serotype 7 while serotype 2 was observed in few isolates. The ma...

During the biological studies of Coccinella septempunctata (Linnaeus) under laboratory conditions on mustard aphid, Lipaphis erysimi (Kalt.). It was revealed that the egg incubation period averaged 3.33 ±0.33 days. The first, second, third and fourth grubs instars respective duration were averaged 2.33±0.33, 2.33±0.33, 3.67±0.33 and 4.67±0.33 days....

He has PDF and achieved 06 research awards in the outstanding contribution of relevant discipline, published 39 research papers, 8 books, 13 book chapters, 28 popular articles, 6 success stories, 45 delivered lecture in different seminar/symposia/conference/ workshop and 5 radio talks. Dr. Hasan organized 244 (9896 trainee) Trainings for Practicing...

The effectiveness of entomopathogenic fungi M. anisopliae with the concentrations of 2 × 10 3 , 2 × 10 4 , 2 × 10 5 , 2 × 10 6 , 2 × 10 7 , 2 × 10 8 , 2 × 10 9 and 2 × 10 10 conidia ml-1 with 0.5 percent Jaggary were evaluated against Helicoverpa armigera (Hub) infestation on chickpea under field condition during 2020-21 and 2021-2022. The larval r...

To understand the pathology of natural cases of E. coli pathotypes infection in bovine calves, 45 cases of bovine calves, below one month of age, died due to enteritis were studied. Total seventeen cases (37.77%) turned positive for different pathotypes of E. coli by RT-PCR. Out of seventeen positive samples for E. coli, six cases (35.29%) were pos...

The present study was carried out for the optimization of protocols of pig urinary bladder acellular matrix using different biological detergents and enzymes. The urinary bladder treated with trypsin enzyme and biological detergents at different concentrations showed more dissolution, and only a few remnants of tissue were left and were not appropr...

Porcine circovirus type 2 (PCV-2) is of great economic significance to porcine industry worldwide. PCV-2 variants and genotypes, alternating world over, are the etiological agent of several clinical syndromes such as porcine dermatitis and nephropathy syndrome (PDNS), post-weaning multi-systemic wasting syndrome (PMWS) and others in pigs. This stud...

Caprine intestinal diseases associated with clostridia are generally caused by Cpa and Etx encoded alpha (α) and epsilon (ε) toxinotypes of Clostridium perfringens type D respectively. A recent study on goat enterocolitis, demonstrated that the incidence of Clostridium perfringens type-D toxinotype and beta 2 toxins is high, suggesting its role in...

Foot-and-mouth disease (FMD) is an extremely contagious and economically devastating viral disease of cloven-hoofed domestic and wildlife animals. The disease is endemic in India and other developing countries of the world. The disease is mainly characterized by the presence of vesicular lesions and “tigroid heart” in calves. The current report des...

The present experiment was carried out during Rabi season, 2020-21 and 2021-22 at Crop Research Centre (CRC), of Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P). Larval population of pod borer was recorded on 10 randomly selected plants of inner rows per plot. The early incidence of larval population was observed at...

Streptococcus suis is an emerging bacterial pathogen of huge economic impact to the swine industry worldwide. The information regarding the carrier status of S. suis in the slaughtered pigs along with its genetic characterization is not available in Indian pig population, which needs to be addressed for the therapeutic and preventive measures. In t...

The book contains lead papers and abstract of the papers presented during 5th International Conference on Advances in Smart Agriculture and Biodiversity Conservation for Sustainable Development organized by Agricultural Technology Development Society (ATDS), Ghaziabad, India and co-partners at Jaipur National University, Jaipur during 4-6 March, 20...

Maedi is a lentiviral disease characterized by progressive interstitial pneumonia with humoural as well as cell mediated immune response. The present investigation was designed to detect the presence of MVV in different biological samples and to evaluate the immune response in naturally MVV infected sheep and goats. Total of 701 biological samples...

The effectiveness of entomopathogenic fungi M. anisopliae with the concentrations of 2 × 10 3 , 2 × 10 4 , 2 × 10 5 , 2 × 10 6 , 2 × 10 7 , 2 × 10 8 , 2 × 10 9 and 2 × 10 10 conidia ml-1 with 0.5 percent Jaggary were evaluated against Helicoverpa armigera (Hub) infestation on chickpea under field condition during 2020-21 and 2021-2022. The larval r...

The present investigation entitled "Age specific life-table of Bactrocera dorsalis under room temperature". The experiments were undertaken at during years 2019. It was the insect required 71 days for the complete its generation at room temperature. The table clearly revealed that a drop in the survivorship (lx) for the first 3 rd days. It again st...

We provide a novel single restriction enzyme (RE) (BsaHI) digestion approach for detecting distinct pathotypes of the Newcastle disease virus (NDV). After scanning 4000 F gene nucleotide sequences in the NCBI database, a single RE (BsaHI) digesting site was discovered in the cleavage site. APMV-I “F gene” Class II specific primer-based reverse tran...

The present experiment was conducted to study the role of cytokine, chemokine and TLRs responses of H9N2-PB2 reassortant H5N1 viruses as compared to non-reassortant H5N1 viruses isolated from crows in BALB/c mice. Two groups (12 mice each) of 6-8 weeks old BALB/c mice were intranasally inoculated with 106 EID50/ml of viruses A/crow/India/03CA04/201...

Background: Spotted pod borer, Maruca vitrata, cause greater damage by attacking flowers, pods in greengram plants. This study investigates the efficacy and economics of certain novel insecticides when sprayed against spotted pod borer in green gram. Methods: The experiment was conducted at Crop Research Center, Sardar Vallabhbhai Patel University...

We provide a novel single restriction enzyme (RE) ( BsaHI ) digestion approach for detecting distinct pathotypes of the Newcastle disease virus (NDV). After scanning 4000 F gene nucleotide sequences in the NCBI database, a single RE ( BsaHI ) digesting site was discovered in the cleavage site. APMV-I “F gene” Class II specific primer-based reverse...

The experiments on population fluctuation of mango fruit fly species were undertaken at the Department of Agricultural Entomology, College of Agriculture, SVPUA & T, Meerut at geographical location 29.0748° N, 77.7082° E, Uttar Pradesh during 2019 and 2020. The population of Bactrocera dorsalis showed its peak in June during 24th meteorological sta...

The present investigation was carried out at Bio-control laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, (U.P.) during the Rabi 2016-17 and 2017-18. The experiment was conducted in Crop Research Centre (CRC), Chirori Farm, Sardar Vallabhbhai Patel University of Agriculture & Technology,...

The pathogenesis of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still not fully unraveled. Though preventive vaccines and treatment methods are out on the market, a specific cure for the disease has not been discovered. Recent investigations and research studies primarily focus on...

The present investigation entitled “Mass production of entomopathogenic fungi Beauveria bassiana (Metsch.) on liquid and solid media” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2018-19. In all there were eight treatments including control repl...

In the present study, fibroblasts seeded matrices prepared from bovine pericardium was evaluated in the repair of full-thickness skin defects in rats. The pericardium tissue from buffalo was treated with 1% sodium dodecyl sulphate (SDS) for 48 hours showed complete acellularity with normal thickness and arrangements of collagen fibres. In-vitro pri...

Mastitis (intramammary inflammation) caused by infectious pathogens is still considered a devastating condition of dairy animals affecting animal welfare as well as economically incurring huge losses to the dairy industry by means of decreased production performance and increased culling rates. Bovine mastitis is the inflammation of the mammary gla...

Indigenous breeds of young chickens in India are believed to be resistant to the classical strain of infectious bursal disease virus (IBDV). However, the mechanism underlying this resistance is obscure. Innate immunity is a key factor in defining the clinical course and pathology of microbial infections. The present study is aimed to compare the pa...

Hamartoma is a developmental disorder characterized by the presence of well-differentiated tissue, of multiple types, in excess at its normal location. The present study describes a fibrous tissue pulmonary hamartoma in two buffaloes. Grossly, there were large pale white masses present on the left diaphragmatic lobe in both cases. Microscopic exami...

Efficacy of different treatments viz., imidacloprid 17.8 SL, buprofezin 25 SC, fipronil 5 SC, spinosad 45 SC, emamectin benzoate 5 SG, Bacillus thuringiensis, and neem oil 1500 ppm was carried out to study the relative efficacy of some newer insecticides and biopesticides against whitefly (Bemisia tabaci Gennadius) in brinjal ecosystem. All the tre...

The coronavirus disease 2019 (COVID-19) pandemic wreaked havoc worldwide, with more than 20 million confirmed cases and nearly 0. 75 million deaths as of 10th August 2020. Various factors determine the severity and symptoms of this infection. Older age and underlying diseases are the challenges being faced in controlling and treating COVID-19. In 2...

Entomopathogenic fungi (EPF) Metarhizium anisopliae are fungal species that is the frequently occurring and destructive pest management to the pathogenic soil and insects. The effect of different substrates for the mass production of Metarhizium anisopliae spore/ml was significantly higher recorded The results revealed that all the treatments were...

Coronavirus Disease 2019 (COVID-19), emerged in early December 2019 in China and became a pandemic situation worldwide by its rapid spread to nearly 190 countries. Bats are considered as the reservoir host, and the search of a probable intermediate host is still going on. The severe form of the infection is associated with death is mainly reported...

Aflatoxins cause significant economic losses in livestock sector and pretense international trade implications. Aflatoxin B1 (AFBI), in particular is the most prevalent form than other aflatoxins. Among livestock,goats are considered to be relatively resistant to aflatoxin. The present report describes a unique case of nervous form of Aflatoxicosis...

This study evaluated the therapeutic action of hydroethanolic extract of Trianthema portulacastrum L. (TPE) on N-nitroso-N-methylurea (NMU)-induced mammary tumors in Wistar rats. A hydroethanolic was prepared and subjected to qualitative and quantitative phytochemical screening. After acclimatization, Wistar rats were divided into 4 groups of 6 rat...

Coronavirus Disease 2019 (COVID-19), caused by a novel coronavirus named Severe Acute Respiratory Syndrome - Coronavirus-2 (SARS-CoV-2), emerged in early December 2019 in China and attained a pandemic situation worldwide by its rapid spread to nearly 167 countries with 287.239 confirmed cases and 11.921 human deaths with a case fatality rate (CFR)...

Pneumonia in bovines is a multifactorial disease manifestation leading to heavy economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPI-3) is are one ofamong the important contributing factors for the development of pneumonia in young animals. These viral agents either primarily cause pneumonia...

In our study we assessed the pathogenicity of two H5N1 viruses isolated from crows in mice. Eighteen 6-8 weeks BALB/c mice each were intranasally inoculated with 106 EID50/ml of H5N1 viruses A/crow/India/03CA04/2015 (H9N2-PB2 reassortant H5N1) and A/crow/India/02CA01/2012 (Non-reassortant H5N1). The infected mice showed dullness, weight loss and ru...

Pasteurella multocida is an economically important respiratory pathogen of pigs confronting swine industry worldwide. Despite extensive research over the decades, its pathogenesis is still poorly understood. Recent reports have demonstrated the nervous system affection as a newer aspect of pathogenesis by Pasteurella multocida type B:2 in Haemorrha...

Peste des petits ruminant (PPR), a highly contagious viral disease of small ruminants, is characterized by erosive stomatitis and pneumo-enteritis. However, its neurovirulence potential as observed with other morbilliviruses has not been fully investigated. The present study describes the neuropathological alterations induced by PPR virus through a...

An epidemiological study was conducted for investigation of bovine coronavirus by antigen Enzyme-linked immunosorbent assay kit in dairy calves from Central India (Madhya Pradesh and Chhattisgarh states) and North India (Uttar Pradesh state). Different epidemiological parameters like age, parity, colour and consistency of faecal materials and seaso...

Studies on pathogenicity of M. anisopliae against Ist - VIth instar H. armigera larvae revealed that larval mortality. The effect of Metarhizium anisopliae spore/ml against Helicoverpa armigera larval instars was significantly higher recorded showed that in the results revealed that all the treatments were significantly larval mortality. The result...

The present investigation was carried out to study the “Eco friendly management of yellow tem borer, Scirpophaga incertulas (Walker) on paddy in western plain zone of Uttar Pradesh” in randomized block design with three replications and seven treatments at Crop Research Center, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meer...

Bovine rotavirus (BRoV) and bovine coronavirus (BCoV) are major enteric viral pathogens responsible for calve diarrhoea. They are widespread both in dairy and beef cattle throughout the world and causing huge economic losses. The diagnosis of these agents is very difficult due to non-specific nature of lesions and the involvement of some intrinsic...

Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is characterized by severe immunosuppression in young chicks of 3 to 6 week age group. Although vaccines are available to prevent IBD, outbreaks of disease are still noticed in the field among vaccinated flocks. Further, the birds surviving IBD become susceptible to...

Mastitis, an inflammation of the udder, is a challenging problem in dairy animals accounting for high economic losses. Disease complexity, degree of economic losses and increasing importance of the dairy industries along with public health concerns envisages devising appropriate diagnostics of mastitis, which can offer rapid, accurate and confirmat...

The present investigation entitled “Mass Production of entomopathogenic fungai Beauveria bassiana (Metsch.) on liquid and solid media” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2018-19. In all there were eight treatments including control rep...

An epidemiological study was conducted for detection of rotavirus by antigen ELISA kit in dairy calves from Central India (Madhya Pradesh and Chhattisgarh states) and North India (Uttar Pradesh state). Different epidemiological parameters like age, sex, parity, colour and consistency of faecal materials and seasonal changes in a year were recorded....

Faecal samples were collected from a total of 148 cattle calves, up to 3 months of age, having clinical diarrhoea during different seasons of the study period from November 2017 to October 2018 from organized farms of Uttar Pradesh. All the samples were screened for the presence of cryptosporidium oocysts by staining the faecal smear by a modified...

Ovine pulmonary adenocarcinoma (OPA) is a retroviral disease of sheep and occurs usually in two forms viz. classical and atypical, which differ in their histological lesions, cellular infiltrates, behaviour and prognosis. Present investigation was focussed to evaluate the differential expression of various tumour biomarkers in both OPA forms. Among...

Nipah (Nee-pa) viral disease is a zoonotic infection caused by Nipah virus (NiV), a paramyxovirus belonging to the genus Henipavirus of the family Paramyxoviridae. It is a biosafety level-4 pathogen, which is transmitted by specific types of fruit bats, mainly Pteropus spp. which are natural reservoir host. The disease was reported for the first ti...

The present investigations were undertaken in laboratory on “Study on factitious host Corcyra cephalonica (Stainton) against storage of egg parasitoid Trichogramma chilonis (Ishii) cards under laboratory conditions” during the year 2018 in the bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Sardar Vallabhbhai...