Rahma Ahmed Nemr- Master of Microbiology
- PhD Candidate at Cairo University
Rahma Ahmed Nemr
- Master of Microbiology
- PhD Candidate at Cairo University
About
15
Publications
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Introduction
Current institution
Publications
Publications (15)
Background
Wheat is a major global crop, and increasing its productivity is essential to meet the growing population demand. However limited water resources is the primary constraint. This study aimed to identify genetic factors associated with drought tolerance using a diverse panel of 287 wheat genotypes evaluated under well-watered and drought-s...
Salinity is a significant factor restricting plant growth and production. The effect of salinity stress on different growth parameters of 111 fenugreek genotypes was examined in an experiment with three salinity levels (0, 3000, 6000 mgL−1). A completely randomized block design with two replicated pots per treatment was used. Non-significant treatm...
Alliances of microbiota with plants are masked by the inability of in vitro cultivation of their bulk. Pure cultures piled in international centers originated from dissimilar environments/hosts. Reporting that plant root/leaf-based culture media support the organ-specific growth of microbiota, it was of interest to further investigate if a plant-ba...
One sentence summary: This study evaluates the ability of clay chips and beads as a practical and cheap methodology to capture the microbiota from barley roots and preserve pure isolates up to 11 months. Downloaded from https://academic.oup.com/femsec/advance-article/doi/10.1093/femsec/fiac064/6596281 by guest on 01 June 2022 Abstract Capturing the...
Capturing the diverse microbiota from healthy and/or stress resilient plants for further preservation and transfer to unproductive and pathogen overloaded soils, might be a tool to restore disturbed plant-microbe interactions. Here, we introduce Aswan Pink Clay as a low-cost technology for capturing and storing the living root microbiota. Clay chip...
Plant microbiota have co-evolved with their associated plants in the entire holobiont, and their assemblages support diversity and productivity on our planet. Of importance is in vitro cultivation and identification of their hub taxa for possible core microbiome modification. Recently, we introduced the in situ-similis culturing strategy, based on...
Plant microbiota support the diversity and productivity of plants. Thus, cultivation-dependent approaches are indispensable for in vitro manipulation of hub taxa. Despite recent advances in high-throughput methods, cultivability is lagging behind other environmental microbiomes, notably the human microbiome. As a plant-based culturing strategy, we...
High-throughput cultivation methods have recently been developed to accelerate the recovery of microorganisms reluctant to cultivation. They simulate in situ environmental conditions for the isolation of environmental microbiota through the exchange of growth substrates during cultivation. Here, we introduce leaf-based culture media adopting the co...
The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating...
Improving cultivability of a wider range of bacterial and archaeal community members, living natively in natural environments and within plants, is a prerequisite to better understanding plant-microbiota interactions and their functions in such very complex systems. Sequencing, assembling, and annotation of pure microbial strain genomes provide hig...
Unculturable populations represent diverse groups of microbes that account for more than 90% of a given ecosystem that are still under shadow. Different approaches were established to culture unculturable bacteria, one of which is diluting culture media to increase culturability. Culture media of 1/10 strength R2A and 1/100 nutrient broth successfu...
In order to improve the culturability and biomass production of rhizobacteria, we previously introduced plant-only-based culture media. We herein attempted to widen the scope of plant materials suitable for the preparation of plant-only-based culture media. We chemically analyzed the refuse of turfgrass, cactus, and clover. They were sufficiently r...
Our previous publications and the data presented here provide evidences on the ability of plant-based culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homog...
