Ragaa HamoudaUniversity of Sadat City GEBRI · Microbial biotechnolgy
Ragaa Hamouda
Professor
About
160
Publications
53,374
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Introduction
Additional affiliations
November 2014 - January 2016
GEBRI University of Sadat City
Position
- Associate Prof University of Sadat City
August 2005 - March 2020
University of Sadat city Egypt
Position
- Professor
Publications
Publications (160)
The exploitation of drought is a critical worldwide challenge that influences wheat growth and productivity. This study aimed to investigate a synergistic amendment strategy for drought using the single and combined application of plant growth-promoting microorganisms (PGPM) (Trichoderma harzianum) and biogenic silica nanoparticles (SiO2NPs) from r...
Chitosan nanoparticles (CNPs) are promising biopolymeric nanoparticles with excellent physicochemical, antimicrobial, and biological properties. In this investigation, CNPs were produced using Ulva fasciata biomass extract as a reducing agent. The SEM micrograph revealed that the biosynthesized CNPs appeared to be spheres with a mean size of 32.49...
Cancer is a threatening disease that needs strong therapy with fewer side effects. A lot of different types of chemotherapy or chemo-drugs are used in cancer treatments but have many side effects. The increasing number of antibiotic-resistant microorganisms requires more study of new antimicrobial compounds and delivery and targeting strategies. Th...
Nanoemulsions (NEMs) are more stable and permeable than regular emulsions because of their increased surface area and smaller particle sizes, which are stabilized by emulsifiers and consist of nanometer-sized droplets. Utilization of an olive oil nanoemulsion (NEM-olive oil) loaded with silver nanoparticles (Ag-NPs) derived from marine alga Turbina...
Background
The climatic changes crossing the world menace the green life through limitation of water availability. The goal of this study was to determine whether Moringa oleifera Lam. trees cultivated under Tunisian arid climate, retain their tolerance ability to tolerate accentuated environmental stress factors such as drought and salinity. For t...
Background and Aim
Natural antioxidants are crucial for preserving and enhancing the health, survival, reproduction, and reproductive function of poultry. Phycocyanin (PC) is a natural blue food colorant with various health benefits. The aim of this study was to extract Arthrospira platensis phycocyanin (ApPC) from A. platensis using simple and eco...
In an attempt to reduce such decay induced by pathogenic causes, several studies investigated the effectiveness of nanoparticles (NPs) that play a vital role in saving food products, especially fruits. Current research delves into biogenic silver nanoparticles (using marine alga Turbinaria turbinata (Tt/Ag-NPs) and their characterization using FT-I...
BACKGROUND
Most microorganisms that cause food decay and the lower the shelf life of foods are fungi. Nanotechnologies can combat various diseases and deal with the application of nanomaterial to target cells or tissues. In this study selenium nanoparticles (Se‐NPs) were synthesized using ascorbic acid and characterized by ultraviolet–visible spect...
The present investigation aims to improve the efficiency of fungal mono- and mixed cultures in removing organic pollutants and metals from sewage water (SW) for further maize plant response assessments. The reduction in the organic load from the SW was harnessed using a co-culture consortium consisting of Aspergillus niger (KB5), Sordariomycetes sp...
The present investigation aims to improve the efficiency of fungal mono-and mixed cultures in removing organic pollutants and metals from sewage water (SW) for further maize plant response assessments. The reduction in the organic load from the SW was harnessed using a co-culture consortium consisting of Aspergillus niger (KB5), Sordariomycetes sp....
Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs were investigated as antioxidant, anticancer, and...
Herein, we used the aqueous extract of Uncaria tomentosa L. barks (Cat’s claw bark [CCb]) for the biofabrication of silver nanoparticles (CCb-Ag-NPs). The effects of different parameters (Uncaria tomentosa L. aqueous extract, silver nitrate [AgNO3] ratio, temperature, and pH) on the formation of the nanoparticles were investigated using UV scan as...
Background and Aim
Global efforts are continuing to develop preparations against cryptosporidiosis. This study aimed to investigate the efficacy of biosynthesized Ulva fasciata loading Cinnamomum camphora oil extract on new zinc oxide nanoparticles (ZnONPs shorten to ZnNPs) and silver nanoparticles (AgNPs) as alternative treatments for Cryptosporid...
Probiotics have appeared a beneficial effects on human wellbeing, the immunomodulatory activities is the most critical mechanism of probiotics, researchers commenced assessing the impact of some immunobiotics, on pathogenic microorganisms as well as on viruses.
Gold nanoparticles (Au-NPs) have several uses for nanobiotechnologists because of their beneficial biomedical properties. Alginates have various biomedical and industrial applications. The aim of this study is to extract alginate from Azotobacter chroococcum, synthesize chemical Au-NPs (Ch/Au-NPs), and load the NPs with the extracted alginate to fo...
Fungal diseases have always been a major problem for cantaloupe crops; however, synthetic fungicides are hazardous to humans and the environment. Consequently, a feasible alternative to fungicides without side effects could be by using bio agents and naturally occurring plants with antibacterial potential. This study has achieved a novel procedure...
Dental caries is an infectious oral disease caused by the presence of different bacteria in biofilms. Multidrug resistance (MDR) is a major challenge of dental caries treatment. Swabs were taken from 65 patients with dental caries in Makkah, Saudi Arabia. Swabs were cultivated on mitis salivarius agar and de Man, Rogosa, and Sharpe (MRS) agar. VITE...
ABSTRACT
Nanoparticles (NPs) are a new, emerging class of nanoscaled materials that are making appreciable revolutions in various fields, intensely in the health sector. As a result of their distinctive physicochemical characteristics, synthesized NPs have gained great attention in various biotechnological sectors, such as the environmental, medica...
A huge amount of textile dyes are released as industrial waste into the environment each year, which alters the water's natural appearance and causes toxicity and carcinogenicity in the human body. Peanut husk is considered an agro-waste and contains many valuable compounds, such as cellulose. Different concentrations of cellulose were extracted fr...
Expression of Concern for ‘Statistical optimization of photo-induced biofabrication of silver nanoparticles using the cell extract of Oscillatoria limnetica: insight on characterization and antioxidant potentiality’ by Rasha A. Abo-Elmagd et al., RSC Adv., 2020, 10, 44232–44246, DOI: https://doi.org/10.1039/D0RA08206F.
Plant-based foods may enhance the prevention of cancer. The present investigation aimed to assess the antigenotoxic effects of chitosan nanoparticles (CNPs) when loaded with the ethanol extract of C. cartilaginea (CNPs/Cc). Synthesis of CNPs and CNPs/Cc and their characterization were carried out using TEM, EDS, DSC, and Zeta potential. For in vivo...
Botanical oils are considered ecofriendly compounds with insecticidal characteristics. In this
study, the effects of basil (Ocimum basilicum), camphor (Cinnamomum camphora), cinnamon
(Cinnamomum verum), lemon (Citrus limon), clove (Syzygium aromaticum), ginger (Zingiber
officinale), black pepper (Piper nigrum), and orange (Citrus sinensis var ba...
Bacterial pathogens cause pain and death, add significantly to the expense of healthcare globally, and pose a serious concern in many aspects of daily life. Additionally, they raise significant issues in other industries, including pharmaceuticals, clothing, and food packaging. Due to their unique properties, a great deal of attention has been give...
ABSTRACT
There are two major problems in the world, fuel deficiency and environmental pollution by fossil
fuels. Microalgae are regarded as one of the most feasible feedstocks for the manufacturing of
biofuels and are used in the degradation of fossil fuel spills. The present study was possessed to
investigate the ability of green alga Chlorell...
Citation: Hamouda, R.A.; Alharbi, A.A.; Al-Tuwaijri, M.M.; Makharita, R.R. The Antibacterial Activities and Characterizations of Biosynthesized Zinc Oxide Nanoparticles, and Their Coated with Alginate Derived from Fucus vesiculosus. Polymers 2023, 15, 2335. https://doi. Abstract: Zinc oxide nanoparticles have many advantages for nano-biotechnologis...
Citation: Gheda, S.; Hamouda, R.A.; Naby, M.A.; Mohamed, T.M.; Al-Shaikh, T.M.; Khamis, A. Potent Effect of Phlorotannins Derived from Sargassum linifolium as Antioxidant and Antidiabetic in a Streptozotocin-Induced Diabetic Rats Model. Appl. Sci. 2023, 13, 4711. https://doi. Abstract: Phlorotannins are phenolic compounds existing in large amounts...
One of the most prevalent chronic infectious disorders is tooth decay. Acids produced when plaque bacteria break down sugar in the mouth cause tooth decay. Streptococcus mutans and Lactobacillus acidophilus are the most prominent species related to dental caries. Innovative biocidal agents that integrate with a biomaterial to prevent bacterial colo...
Several types of green photosynthetic microalgae can grow through the process of heterotrophic growth in the dark with the help of a carbon source instead of the usual light energy. Heterotrophic growth overcomes important limitations in the production of valuable products from microalgae, such as the reliance on light, which complicates the proces...
Over the last decade, an extensive range of consumer products containing manufactured silver nanoparticles (AgNPs) have been progressively used. The unfitting usage and discharge of these materials can enable passage of AgNPs into the aquatic ecosystem causing prospective toxicological consequence. The present study shed new lights on the phycotoxi...
The scaling up and increment of the algal cultures cultivation process is a complex task that requires
experienced staff. Some parameters such as biomass yield, biomass productivity, and specific growth
should be calculated using the findings of laboratory scale that might be relevant for large-scale production
as it provides a baseline to visualiz...
Dhiba port has a strategic location near the Neom project. Various anthropogenic activities contributed to the discharge of metals, metalloids and oil spills in the aquatic system and caused environmental pollution. Microalgae are the best microorganisms in aquatic conditions known to be capable of eliminating contaminants. In this work the Chlorel...
In biological engineering, cell immobilization is a modern technique for immobilizing free cells in a small space. Disintegration and elimination of azo dyes [Reactive Orange 122 (orange 2RL) and Reactive Red 194 (Reactive Red M-2BF)] were investigated by using Chlorococcum sp. and Chlorococcum sp. mixed with Scenedesmus obliquus, respectively. Aft...
Salt stress is one of the most serious factors that influence the plant growth and yield. The extensive use of chemical fertilizers caused an increase in land contamination and hence effects on human health. The use of biofertilizers can solve these problems. In the present work, different concentrations of Spirulina platensis liquid extracts (SLEs...
The enormous industrial usage of nickel during its manufacture and recycling has led to widespread environmental pollution. This study was designed to examine the ability of Gelidium amansii biomass to biosorb Ni ²⁺ ions from an aqueous solution. Six independent variables, including contact time (1.0 and 3.0 h), pH (4 and 7), Ni ²⁺ concentration (2...
Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirming its...
Hyperlipidemia is a significant risk factor for atherosclerosis and coronary heart disease (CHD). The aim of this study was to investigate hypolipidemic effects of Lactobacillus, Spirulina and their combination on Swiss albino mice fed a regular or high-cholesterol diet. Rosuvastatin was used as a reference drug The highest body weight, total chole...
Microalgae are a diverse group of microorganisms, the majority of which are photosynthetic in nature. Microalgae have different applications, the most important of which is the biological treatment of wastewater. Microalgae grow in various types of wastewater, such as wastewater polluted by Azo dyes, due to microalgae using wastewater as a culture...
Spirulina platensis (SP) has a high impact on multidiscipline usage worldwide. Vitamins are considered as growth promoters due to their metabolic bio-regulating roles. This study was conducted to investigate the effect of vitamins: riboflavin (B2), thiamine (B1), and ascorbic acid (C) on SP growth and contents of pigments, phenols, and phytochemica...
Poultry meat is a great source of protein and provides lots of nutrients such as iodine, iron, zinc, vitamins, and essential fatty acids that humans require. The positive applications of metal nanoparticles (NPs) in the diets of various poultry species were studied, in relation to their metabolic, antibacterial effects on digestion and regulation o...
Cellulose nanofibers have a highest promise of nanocomposites in many different fields. Nanocomposites are systems in, which at least one component in the nanorange needs to be present. In different types of composites, including the most recent nanocomposites, cellulose is used. Cellulose is a renewable, higher plant-based, algae-based, and bacter...
There is a very vital antioxidant extracted from microgreen alga. Chlorella vulgaris has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), and Ascorbic acid (c) a...
Seaweeds can play a vital role in plant growth promotion. Two concentrations (5 and 10 mg/mL) of soluble polysaccharides extracted from the green macroalgae Ulva fasciata and Ulva lactuca were tested on Zea mays L. The carbohydrate and protein contents, and antioxidant activities (phenols, ascorbic, peroxidase, and catalase) were measured, as well...
Algae are considered as one of the plant growth promoters and have the potential to mitigate stress due to pesticide, salt and climate change. Two field trials of cultivation of Jerusalem artichoke were carried out in spring seasons 2018 and 2019 to select the convenient growing season for possible higher production of inulin content of Jerusalem a...
The main components of petroleum oil are petroleum hydrocarbons, increasing population and modernization, which cause increasing environmental pollution by petroleum hydrocarbon. This chapter is intended to demonstrate the bioremediation using live microbes and their derived as an ecofriendly technique to detoxify or degrade crude oil and petroleum...
Bagasse and wheat bran are agrowaste and cause different environmental problems. These compounds contain highly valuable compounds that can be recycled by microorganisms. This work was carried out to evaluate the effect of fermentation by Bacillus subtilis on biological activity and chemical components of bagasse and wheat bran. The results demonst...
Spirulina platensis is a biomass related to cyanobacteria, cultivated in worldwide due to high major advantage to human, such as dietary supplement or whole food, feed additive in the aquaculture, aquarium, and poultry, and it has immunomodulation, antioxidant, anti-microbial properties. So the world searches to enhancement the S. platensis biomass...
Mitomycin C (MMC) is an alkylating chemotherapy drug that could induced DNA damage, and genetic alteration. It is used as a model mutagen for in vivo and in vitro studies. The current study aimed to evaluates the protective role of ZnO-Algin/NCMs against MMC-induced genotoxicity in mice. Animals were treated as follow: the control group, the groups...
Mitomycin C (MMC) is an alkylating chemotherapy drug that could induced DNA damage, and genetic alteration. It is used as a model mutagen for in vivo and in vitro studies. The current study aimed to evaluates the protective role of ZnO-Algin/NCMs against MMC-induced genotoxicity in mice. Animals were treated as follow: the control group, the groups...
The enrichment of plant rhizosphere microorganisms as Fluorescent Pseudomonas is a strategy that enhances the vegetables production and control pre and post - harvest diseases. Three Pseudomonas spp., (P. fluorescence, P. putida, P. aeruginosa) and their combination are used as biofertilizer to increase the production of Cantaloupe and as biocontro...
Remazol brilliant blue (RBB) is an anthraquinone anionic dye that has several commercial uses, especially in the textile industries and is well-known for its detrimental impacts on marine life and the surrounding ecosystem. Mercury (Hg²⁺) is also one of the most severe hazardous environmental contaminants due to its bioaccumulation through the food...
Therapeutic selectivity is a critical issue in cancer therapy. As a result of its adjustable physicochemical characteristics, the Au/cellulose nanocomposite currently holds a lot of potential for solving this challenge. This work was designed to prepare a Au/cellulose nanocomposite with enhanced anticancer activity through the regulation of the mit...
Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirming its...
We appraised the use of zinc oxide nanoparticles, (ZnO-NPs) and zinc oxide bulk (ZnO-bulk) or zinc acetate, as a natural nematocide, alone or in combination with oxamyl in vitro and in vivo trials in order to improve systems for root-knot nematode (RKNs) control in banana plants. Especially, ZnO-NPs were biosynthesized from the alga, Ulva fasciata....
Because of the increased amount of cobalt and Congo red dye effluents attributable to the industrial operations, the capacity of Enteromorpha intestinalis biomass as a sustainable source to achieve significant biosorption percent for both pollutants from dual solution was assessed. A fifty batch FCCCD experiments for biosorption of cobalt ions and...
Nosocomial bacteria's ability to produce biofilms helps them survive in a variety of environments, such as hospitals, wounds, and medical devices. Cellulases can destroy cellulose, one of the major structural components of biofilms, representing an important part of the bacterial biofilm matrix. Bacterial strains were obtained from diabetic foot ho...
Endoglucanase producing bacteria were isolated from Egyptian soils and the most active bacterial strain was identified as Bacillus subtilis strain Fatma/1. Plackett–Burman statistical design was carried out to assess the effect of seven process variables on endoglucanase production. Carboxymethyl cellulose (CMC), yeast extract and peptone were the...
Cephalosporium maydis is a soil borne and wide spread fungus in Egypt causing Late Wilt disease in Maize (Zea mays). This study tested methanol extracts of some marine algae such as Jania rubens, Corallina elongata, Laurencia obtusa, red algae as Gelidium crinale, green algae as Enteromorpha compressa, Ulva fasciata and cyanobacreria as Anabaena or...
Cyanobacteria are microscopic microorganisms, unicellular or multicellular oxygenic photoautotrophs, and Gram-negative prokaryotes, widespread in terrestrial or aquatic environment and mostly occur in fresh water and marine environment. They have greatest diversity and morphology among all prokaryotes. They are the principal producers of biomass in...
Paracetamol is extensively consumed as an analgesic and antipyretic drug, but at a high dose level, it leads to deleterious side effects, such as hepatic and nephrotoxicity. This research aimed to estimate the prophylactic efficacy of Chlorella vulgaris and/or thiamine against paracetamol (P) induced hepatorenal and cardiac toxicity. Forty-eight fe...