Raffaele Testolin

Raffaele Testolin
University of Udine | UNIUD · Department of Agricultural and Environmental Sciences

MD Plant Science

About

223
Publications
58,363
Reads
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7,973
Citations
Citations since 2017
42 Research Items
2940 Citations
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Introduction
Raffaele Testolin currently works at the Department of Agricultural and Environmental Sciences, University of Udine. Main interests: (a) isolation of molecular markers in fruit crops (kiwifruit, peach, almond, apricot, olive, grape, strawberry) and their use in population genetics and germplasm management; (b) fruit crop genetics and breeding (kiwifruit, grape, apple, apricot, olive) including mapping and cloning genes of interest for breeding.
Additional affiliations
September 2015 - January 2016
University of Udine
Position
  • Professor (Full)
Description
  • Fruit Science and Genetic Resources in Agriculture
June 1981 - present
University of Udine
Position
  • Professor (Full)
Description
  • Professor of Fruit Science and co-founder of the Institute of Applied Genomics (IGA). Expertise: molecular markers and their use in population genetics, and germplasm; kiwifruit, grape, and apple genetics and breeding.

Publications

Publications (223)
Article
The DNA fingerprinting of fruit crops, based on DNA microsatellite markers which are considered to be the key markers for the molecular analysis of germplasm collections, is reviewed. Single sequence repeats (SSRs) remain the markers of choice for fingerprinting in humans, animals, plants, and other living organisms. This review, that considers 44...
Article
Full-text available
Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistances against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinif...
Article
Full-text available
Dioecism and an extended juvenile phase of 3–7 years in kiwifruit hinder the progress in breeding new cultivars. The identification of fruit-bearing females at an early stage of growth is crucial for breeders. Consequently, molecular markers have become a key tool for identifying female and male plants at an early stage of development. Several effo...
Article
Full-text available
Apple breeding is active worldwide and yet the apple crop is in a precarious state as it relies on few dominant cultivars and only the Rvi6 (formerly Vf) gene, that confers resistance to scab, has been extensively exploited in the cultivars entered the market in recent years. However, there are some 20 disease resistance genes described in apple an...
Preprint
Full-text available
Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistance against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinife...
Article
Full-text available
Kiwifruit belong to the genus Actinidia with 54 species apparently all functionally dioecious. The sex-determinants of the type XX/XY, with male heterogametic, operate independently of the ploidy level. Recently, the SyGI protein has been described as the suppressor of female development. In the present study, we exploited the CRISPR/Cas9 technolog...
Article
Full-text available
Nutritional unbalances, such as calcium deficiency at the fruit level, are generally the causative agent of post-harvest disorders in apples. Foliar application of Ca as calcium chloride is the current solution to increase Ca concentration in apples, even though the effectiveness of this approach is often not satisfactory. In this research, we test...
Article
Full-text available
Ancient germplasm represents a reservoir of traits and genes that might maintain large the genetic diversity of a species, like pear, and help breeders to cope with climatic changes and the evolving demand of the market. This paper reports the analysis of 170 pear accessions profiled at 12 simple sequence repeat (SSR) markers. The collection was re...
Article
Full-text available
Sharka, a common disease among most stone fruit crops, is caused by the Plum Pox Virus (PPV). Resistant genotypes have been found in apricot (Prunus armeniaca L.), one of which—the cultivar ‘Lito’ heterozygous for the resistance—has been used to map a major quantitative trait locus (QTL) on linkage group 1, following a pseudo-test-cross mating desi...
Article
A wild grape haplotype (Rpv3‐1) confers resistance to Plasmopara viticola. We mapped the causal factor for resistance to an interval containing a TIR‐NB‐LRR (TNL) gene pair that originated 1.6‐2.6 million years ago by a tandem segmental duplication. Transient coexpression of the TNL pair in Vitis vinifera leaves activated pathogen‐induced necrosis...
Conference Paper
Full-text available
Friuli Venezia Giulia is a rich reservoir of walnut (Juglans regia L.) germplasm, representing a corridor that links the Balkans to the Italian peninsula, the two germplasm sources that met during re-colonization after the last glaciation. The analysis of genetic diversity of 215 wild accessions collected in the region and genotyped with 20 microsa...
Article
Full-text available
Key message The developmental morphology of male and female kiwifruit flowers is tracked to delimit a framework of events to aid the study of divergence in floral gene expression. Abstract The transition from hermaphrodite to unisexual development of kiwifruit (Actinidia chinensis Planch) flowers has been reported previously, but differences in ge...
Article
This paper reports the genetic diversity of apple germplasm collected in the Friuli Venezia Giulia region (north-eastern Italy). The collection, maintained in three different locations, was represented by local cultivars which probably originated as chance seedlings together with cultivars introduced from neighbouring countries, the name and origin...
Conference Paper
Full-text available
Since 1998 at University of Udine started a breeding program with the purpose of introgressing resistance genes into elite wine cultivars. The first ten varieties were released in collaboration with the Institute of Applied Genomics and introduced into the market by Vivai Cooperativi Rauscedo, one of the leading grape nurseries. Here we present the...
Article
Full-text available
Background: Most published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft genome has 164 Mb...
Article
Nuts collected from wild accessions of Persian walnut (Juglans regia L.) in the Friuli Venezia Giulia region (North Eastern Italian Alps) were evaluated during 2013–2015. The analyses carried out were mainly on fruit traits, the only morphological traits that could be observed and statistically analysed, being less dependent on the area of sampling...
Article
Background: walnut oil use is currently limited by its poor oxidative stability due to the high percentage of polyunsaturated fatty acids. Modifying the oil composition may be a goal in walnut breeding to increase interest in this crop. Exploring natural variability and identifying the main environmental factors affecting oil quality are necessary...
Article
Full-text available
Juglans regia L. is distributed primarily across temperate and subtropical regions of the Northern Hemisphere. During the last glaciation, the species survived in refugial areas that in Europe included the Balkans and the Italian peninsula, two areas joined by a corridor represented by the Friuli Venezia Giulia region, where two germplasm reservoir...
Chapter
In 2013, kiwifruit joined the list of sequenced plant genomes. Its sequence is now a valuable tool for scientists working in different fields, from taxonomy to plant breeding and physiology. As kiwifruit is dioecious and therefore highly heterozygous, scientists encountered severe difficulties in correctly assembling the contigs and, as time goes b...
Chapter
The development of molecular markers in kiwifruit followed the advances in molecular biology techniques. After occasional use of RFLP (restriction fragment length polymorphism) markers in the 1990s, most markers developed in kiwifruit were based on PCR (polymerase chain reaction). RAPD (random amplified polymorphic DNA) were followed by AFLPs (ampl...
Book
This book describes the basic botanical features of kiwifruit and its wild relatives, reports on the steps that led to its genome sequencing, and discusses the results obtained with the assembly and annotation. The core chapters provide essential insights into the main gene families that characterize this species as a crop, including the genes cont...
Chapter
Grapevine breeding has been actively conducted in Italy since the end of the nineteenth century, producing approximately 800 varieties (rootstocks, wine and table grapes). The major Italian rootstock, wine and table grape breeders are listed, including the main varieties they obtained, such as Pirovano (cv. Italia), Paulsen (1103P rootstock), Dalma...
Article
Full-text available
Kiwifruit breeding still largely relies on phenotypic observation of cross progeny grown in the field to fruiting maturity, without any selection prior to the juvenility being overcome. Developing markers for the selection of traits of interest would greatly help breeders to rapidly screen breeding populations. With the aim of mapping several trait...
Article
Full-text available
Russeting is a disorder developed by apple fruits that consists of cuticle cracking followed by the replacement of the epidermis by a corky layer that protects the fruit surface from water loss and pathogens. Although influenced by many environmental conditions and orchard management practices, russeting is under genetic control. The difficulty in...
Article
Full-text available
In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generatio...
Conference Paper
Full-text available
Autochthonous cultivars are a resource for viticulture linked to territory. However they are prone to diseases like all V. vinifera cultivars. A breeding program has been set up in 1998 at the University of Udine, region Friuli, north-eastern Italy to obtain new grapevine resistant selections with high quality of grapes and wine. Up to date more th...
Chapter
Grapevine is a major horticulture crop grown on ~7.6 million hectares that secure a yearly production of ~70 million tons of grapes. A significant part of the crop (65 %) annually fuels a worldwide wine industry of ~27 billion liters. In 2007, 2.8 billion liters in wine sales in the United States alone were worth US$ 30 billion. Grapevines and wine...
Article
Full-text available
For thousands of years, olive trees (Olea europaea L.) have been a significant presence and a symbol in the Garden of Gethsemane, a place located at the foot of the Mount of Olives, Jerusalem, remembered for the agony of Jesus Christ before his arrest. This investigation comprises the first morphological and genetic characterization of eight olive...
Article
Grapevine (. Vitis vinifera L.) encompasses a staggering complexity of biochemical variation in the fruit of varieties that individually differ by nuances of colour, taste and aroma. A deep understanding of the underlying DNA variation unlocks the evolutionary relationships between varieties, their somatic mutants and their living wild relatives, a...
Article
Full-text available
The Amur grape (Vitis amurensis Rupr.) thrives naturally in cool climates of Northeast Asia. Resistance against the introduced pathogen Plasmopara viticola is common among wild ecotypes that were propagated from Manchuria into Chinese vineyards or collected by Soviet botanists in Siberia, and used for the introgression of resistance into wine grape...
Data
Microsatellite and SNP markers on chr14. (JPG)
Data
Full-text available
Validation of the Rpv12 genetic interval in progeny derived from the cross ‘Kunbaràt’ x ‘Sarfeher’. (PDF)
Data
Description of the Rpv12 region in the reference genome, in the Rpv12+ resistant haplotype, and diversity among grapevines: (panel A) recombination in custom-made populations; (panel B) variable sites between Rpv12+ and V. vinifera haplotypes in six single-copy genes at symmetrical positions with respect to Rpv12 (the analysis was extended to addit...
Data
Introgression of the Rpv12+ region in grapevine varieties and the surrounding chromosomal landscape. Genetic (panel A) and physical (panels D,H) distance of mapped markers; density of exons, class I and II TEs, unclassified high-copy sequences, and low-copy DNA (panel C); density of NB-LRR genes (panels E,I) and their phylogenetic relationships (pa...
Data
DNA fingerprinting and kinship of six accessions claimed to belong to the first filial generation of V. amurensis x V. vinifera crosses. (JPG)
Data
Pedigrees of custom-made populations for genetic mapping, and varieties containing the resistant haplotype of Rpv12. (JPG)
Data
Genetic maps and QTL plots for downy mildew foliar resistance (OIV452 parameter) in an Rpv12+/Rpv12− Rpv3−/Rpv3− resistant parent (panels A and C) and in an Rpv12+/Rpv12− Rpv3+/Rpv3− resistant parent (panels B and D). For the sake of simplicity, cosegregating markers are shown only once on the x-axis of the QTL plots. (JPG)
Data
List of 200 grapevine accessions and their genotypes at the Rpv12 locus. DNA typing was performed by Sanger sequencing of the gene fragments GSVIVT00036207001 and GSVIVT00023546001 and by scoring nucleotide variants at the chromosomal positions chr14:9036110 and chr14: 9882587, flanking the Rpv12 NB-LRR cluster. (JPG)
Data
DNA fingerprinting and kinship of 48 accessions of V. amurensis, descendents of V. amurensis x V. vinifera crosses with unknown pedigree, and introgression lines in the first two generations of descent with known pedigree. (JPG)
Article
Full-text available
Rosaceae is the most important fruit-producing clade, and its key commercially relevant genera (Fragaria, Rosa, Rubus and Prunus) show broadly diverse growth habits, fruit types and compact diploid genomes. Peach, a diploid Prunus species, is one of the best genetically characterized deciduous trees. Here we describe the high-quality genome sequenc...
Conference Paper
Full-text available
Genome analysis based on next generation sequencing (NGS) technologies provides a novel approach for surveying molecular diversity among individuals, which in turn can generate tools for linkage and association mapping, gene cloning, molecular breeding, population genetics, germplasm management, and crop systematics and evolution. 'De novo' assembl...
Article
Full-text available
The sustainability of agricultural production systems is a recurrent topic in the public debate, tightly linked to resource use efficiency and energy saving principles. Higher yield with lower input is the paradigm that will guide us in the years to come. This will unavoidably call for a joint effort of different players from research, production a...
Article
Evaluation of parental genotypes for their general and specific combining ability (GCA and SCA) allows selection of good parents in mating designs that can help breeding programs succeed and produce valuable progeny. We developed 84 controlled crosses arranged in three North Carolina Model 2 (NCM2) mating designs, in which tetraploid A. chinensis f...
Article
Full-text available
Kiwifruit breeding has been supported in the past by the genetic analysis of breeding value of parents based on dissection of the components of variance. Data were collected from biparental mating designs such as the North Carolina Model 2 (NCM2) that is well suited to dioecious species such as kiwifruit. In the last decade we assisted the first at...
Article
Full-text available
The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A selective sweep at this locus was revealed by the DNA genotyping of 580 grapevines, which include a highly diverse set of 265 European varieties that predated the spread of North American mildews, 82 accessions of wild species, and 233 registered breeding...
Article
Full-text available
The Rpv3 locus determines the ability to operate an isolate-specific hypersensitive response (HR) against Plasmopara viticola in grapevines that carry a resistant Rpv3 (+) haplotype. Artificial infection was performed on leaf discs of Rpv3 (+) and Rpv3 (-) grapevines with two distinct isolates of the pathogen (avrRpv3 (+) and avrRpv3 (-)). The plan...