Raffaele Spanò

Raffaele Spanò
Istituto Italiano di Tecnologia | IIT · D3 PharmaChemistry

Ph.D., M.Sc.

About

26
Publications
4,084
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
446
Citations
Introduction
Raffaele Spanò currently works in the Nanotechnology for Precision Medicine group at the D3 PharmaChemistry, Istituto Italiano di Tecnologia. The most recent publication is 'Sub-Lethal Concentrations of Graphene Oxide Trigger Acute-Phase Response and Impairment of Phase-I Xenobiotic Metabolism in Upcyte® Hepatocytes'.
Additional affiliations
January 2012 - December 2015
Università degli Studi di Genova
Position
  • PhD Student

Publications

Publications (26)
Article
Full-text available
The impact of graphene oxide on hepatic functional cells represents a crucial evaluation step for its potential application in nanomedicine. Primary human hepatocytes are the gold standard for studying drug toxicity and metabolism; however, current technical limitations may slow down the large-scale diffusion of this cellular tool for in vitro inve...
Article
The storied history of controlled the release systems has evolved over time; from degradable drug-loaded sutures to monolithic zero-ordered release devices and nano-sized drug delivery formulations. Scientists have tuned the physico-chemical properties of these drug carriers to optimize their performance in biomedical/pharmaceutical applications. I...
Article
Full-text available
The poor transport of molecular and nanoscale agents through the blood–brain barrier together with tumour heterogeneity contribute to the dismal prognosis in patients with glioblastoma multiforme. Here, a biodegradable implant (μMESH) is engineered in the form of a micrometre-sized poly(lactic-co-glycolic acid) mesh laid over a water-soluble poly(v...
Preprint
Full-text available
Background Graphene Oxide (GO) is a promising candidate for nanomedicine applications. Due to the central role of liver in biotransformation of xenobiotics and drugs, the impact of GO on hepatic functional cells represents a crucial evaluation step for its potential implementation as drug. Primary human hepatocytes (PHH) are the election model for...
Article
Full-text available
Here we present a novel, compact 3D-printed diffusion cell as an in vitro tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 h incubation in the cell is d...
Article
After a skin injury, many complex metabolic events are triggered to ensure proper wound healing. Particularly for chronic, non-healing wounds or burns several risk factors such as persistent bacterial infections and fast dehydration can counteract the healing process. Intelligent wound dressings should help accelerate the healing process, while mai...
Conference Paper
Here we present a novel, compact 3D-printed diffusion cell as an in vitro tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 hours incubation in the cell...
Article
Full-text available
The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental autoimmune encephalomyelitis (EAE), the animal model for multip...
Article
Water insoluble monohydrochloride monohydrate free ciprofloxacin (Cipro) antibiotic was incorporated in polyvinylpyrrolidone (PVP) polymer matrix by using acetic acid co-solvent in water. The resultant solutions were cast into fully transparent antimicrobial films. Proper concentrations of acetic acid eliminated in situ crystallization of the antib...
Article
Abstract Water insoluble monohydrochloride monohydrate free ciprofloxacin (Cipro) antibiotic was incorporated in polyvinylpyrrolidone (PVP) polymer matrix by using acetic acid co-solvent in water. The resultant solutions were cast into fully transparent antimicrobial films. Proper concentrations of acetic acid eliminated in situ crystallization of...
Conference Paper
Full-text available
W ound dressings have been evolving steadily in close conjunction with recent trends and advances in bio-based polymer processing. Dressings grew from materials or tapes that simply covered and concealed the wound, to materials that can interact with wounds so that important wound healing factors such as moisture management, active ingredient deliv...
Article
Full-text available
The investigation of the neuronal network in mouse spinal cord models represents the basis for the research on neurodegenerative diseases. In this framework, the quantitative analysis of the single elements in different districts is a crucial task. However, conventional 3D imaging techniques do not have enough spatial resolution and contrast to all...
Article
Chronic skin ulcers, consequence of diabetes and other pathological conditions, heavily compromise the patients' life quality and represent a high and constantly growing cost for National Health Services. Autologous Platelet Rich Plasma (PRP), has been proposed to treat these lesions. The absence of guidelines for the PRP production and the need of...
Article
Full-text available
High resolution Synchrotron-based X-ray Phase Contrast Tomography (XPCT) allows the simultaneous detection of three dimensional neuronal and vascular networks without using contrast agents or invasive casting preparation. We show and discuss the different features observed in reconstructed XPCT volumes of the ex vivo mouse spinal cord in the lumbo-...
Article
Full-text available
In the biomedical sector the availability of engineered scaffolds and dressings that control and reduce inflammatory states is highly desired, particularly for the management of burn wounds. In this work, we demonstrate for the first time, to the best of our knowledge, that electrospun fibrous dressings of poly(octyl cyanoacrylate) (POCA) combined...
Article
Full-text available
The understanding of structure-function relationships in normal and pathologic mammalian tissues is at the basis of a tissue engineering (TE) approach for the development of biological substitutes to restore or improve tissue function. In this framework, it is interesting to investigate engineered bone tissue, formed when porous ceramic constructs...
Article
Faults in vascular (VN) and neuronal networks of spinal cord are responsible for serious neurodegenerative pathologies. Because of inadequate investigation tools, the lacking knowledge of the complete fine structure of VN and neuronal system represents a crucial problem. Conventional 2D imaging yields incomplete spatial coverage leading to possible...
Article
Lipocalin-2 (LCN2) is a protein largely expressed in many tissues, associated with different biological phenomena such as cellular differentiation, inflammation and cancer acting as a survival/apoptotic signal. We found that LCN2 was expressed during osteoblast differentiation and we generated transgenic (Tg) mice over-expressing LCN2 in bone. Tg m...
Article
Full-text available
Abstract Serum of animal origin and in particular fetal bovine serum is the most commonly utilized cell culture medium additive for in vitro cell growth and differentiation. However, several major concerns have been raised by the scientific community regarding the use of animal sera for human cell-based culture applications. Among the possible alte...

Network

Cited By